Conformation-dependent translocation of a star polymer through a nanochannel

The translocation process of star polymers through a nanochannel is investigated by dissipative particle dynamics simulations. The translocation process is strongly influenced by the star arm arrangement as the polymer enters the channel, and a scaling relation between the translocation time τ and the total number of beads N_tot is obtained. Qualitative agreements are found with predictions of the nucleation and growth model for linear block co-polymer translocation. In the intermediate stage where the center of the star polymer is at the channel entrance, the translocation time is found to have power law-dependence on the number of arms outside the channel and very weakly dependent on the number of arms in the channel. Increasing the total number of star arms also increases the star translocation time.     

Modeling the relaxation time of DNA confined in a nanochannel

Using a mapping between a Rouse dumbbell model and fine-grained Monte Carlo simulations, we have computed the relaxation time of λ-DNA in a high ionic strength buffer confined in a nanochannel. The relaxation time thus obtained agrees quantitatively with experimental data [Reisner et al., Phys. Rev. Lett. 94, 196101 (2005)] using only a single O(1) fitting parameter to account for the uncertainty in model parameters. In addition to validating our mapping, this agreement supports our previous estimates of the friction coefficient of DNA confined in a nanochannel [Tree et al., Phys. Rev. Lett. 108, 228105 (2012)], which have been difficult to validate due to the lack of direct experimental data. Furthermore, the model calculation shows that as the channel size passes below approximately 100 nm (or roughly the Kuhn length of DNA) there is a dramatic drop in the relaxation time. Inasmuch as the chain friction rises with decreasing channel size, the reduction in the relaxation time can be solely attributed to the sharp decline in the fluctuations of the chain extension. Practically, the low variance in the observed DNA extension in such small channels has important implications for genome mapping.     

Tandem array of nanoelectronic readers embedded coplanar to a fluidic nanochannel for correlated single biopolymer analysis

We have developed a two-step electron-beam lithography process to fabricate a tandem array of three pairs of tip-like gold nanoelectronic detectors with electrode gap size as small as 9 nm, embedded in a coplanar fashion to 60 nm deep, 100 nm wide, and up to 150 μm long nanochannels coupled to a world-micro-nanofluidic interface for easy sample introduction. Experimental tests with a sealed device using DNA-protein complexes demonstrate the coplanarity of the nanoelectrodes to the nanochannel surface. Further, this device could improve transverse current detection by correlated time-of-flight measurements of translocating samples, and serve as an autocalibrated velocimeter and nanoscale tandem Coulter counters for single molecule analysis of heterogeneous samples.     

Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil

BackgroundSuper-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.ResultsIn this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEX-SPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FU-SPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and −45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.ConclusionsThe findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.How to cite: Mahdia SA, Kadhimb AA, Albukhaty S, et al. Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.001     

潜心“催化”演绎“精彩”

本文基于具体案例探讨了在复习课教学中教师应该如何添加一些“催化元素”,以激发学生参与学生的兴趣,从而演绎出复习课中的精彩。     

Propagation through a slab of irregularities in a magneto-ionic medium

A theory is developed to describe the propagation of characteristics waves through an infinite slab of irregularities in a magneto-ionic medium. The theory makes use of the asymptotic Green's dyadic. The results show that mode conversion is possible through scattering from irregularities in an anisotropic background. Some special cases are discussed and an example is given.     

一题多变 突破一类圆锥曲线定值问题

圆锥曲线中的定值问题一直是高考的热点和难点。本文通过一道例题对此类问题做深度解析,探索其规律,希望以此增强学生思维的深刻性,培养学生的创新精神。     

Pressure-driven transport of particles through a converging-diverging microchannel

Pressure-driven transport of particles through a symmetric converging-diverging microchannel is studied by solving a coupled nonlinear system, which is composed of the Navier–Stokes and continuity equations using the arbitrary Lagrangian–Eulerian finite-element technique. The predicted particle translation is in good agreement with existing experimental observations. The effects of pressure gradient, particle size, channel geometry, and a particle’s initial location on the particle transport are investigated. The pressure gradient has no effect on the ratio of the translational velocity of particles through a converging-diverging channel to that in the upstream straight channel. Particles are generally accelerated in the converging region and then decelerated in the diverging region, with the maximum translational velocity at the throat. For particles with diameters close to the width of the channel throat, the usual acceleration process is divided into three stages: Acceleration, deceleration, and reacceleration instead of a monotonic acceleration. Moreover, the maximum translational velocity occurs at the end of the first acceleration stage rather than at the throat. Along the centerline of the microchannel, particles do not rotate, and the closer a particle is located near the channel wall, the higher is its rotational velocity. Analysis of the transport of two particles demonstrates the feasibility of using a converging-diverging microchannel for passive (biological and synthetic) particle separation and ordering.     

磁纳米颗粒材料研究进展

随着纳米技术的发展及各种纳米材料的广泛应用,磁纳米材料因其多功能性和特殊性,在各个学科领域都引起了人们的广泛兴趣。文章将从磁纳米颗粒的发展历程、研究进展、以及展望三方面进行了阐述。     

Heat transfer in a dipolar flow through a porous channel

The theory of constitutive equations for dipolar fluids, obtained by Bleustein and Green, is applied to investigate the Couette and Poiseuille flows between parallel plates maintained at constant but different temperatures in addition to being subjected to uniform injection and suction. Explicit expressions for the velocity and the temperature fields are obtained. It is found that different sets of conditions imposed on the flow parameters lead to different expressions for the velocity distribution which are valid only for restricted ranges of the cross-flow Reynolds number R. A table showing the various conditions imposed on the parameters, the corresponding solutions and the ranges of R for which the solution exist is presented. Velocity and temperature profiles for the dipolar and the Newtonian flows are drawn and compared to bring out the important differences resulting from the variations in R and B, the Brinkman number. For the dipolar Couette flow it is found that the value of B at which a transition from cooling to heating of the suction wall occurs always exceeds its corresponding value for Newtonian flow. Tables comparing the rates of heat transfer at the walls are provided for several values of R and B.     

Flow characterization of electroconvective micromixer with a nanoporous polymer membrane in-situ fabricated using a laser polymerization technique

Electroconvection is known to cause strong convective mixing in a microchannel near a nanoporous membrane or a nanochannel in contact with an electrolyte solution due to the external electric field. This study addresses micromixer behavior subject to electroconvection occurring near a nanoporous membrane in-situ fabricated by a laser polymerization technique on a microfluidic chip. We found that the micromixer behavior can be categorized into three regimes. Briefly, the weak electroconvection regime is characterized by weak mixing performance at a low applied voltage and KCl concentration, whereas the strong electroconvection regime has a high mixing performance when the applied voltage and KCl concentration are moderately high. Finally, the incomplete electroconvection regime has an incomplete electric double-layer overlap in the nanopores of the membrane when the electrolyte concentration is very high. The mixing index reached 0.92 in the strong electroconvection regime. The detailed fabrication methods for the micromixer and characterization results are discussed in this paper.     

Synthesis of silver nanoparticles using a biosurfactant produced in low-cost medium as stabilizing agent

BackgroundA biosurfactant produced by Pseudomonas aeruginosa cultivated in a low-cost medium formulated with 2.5% vegetable oil refinery residue and 2.5% corn steep liquor and distilled water was employed to stabilize silver nanoparticles in the liquid phase. The particles were initially synthesized using NaBH₄ as reducing agent in biosurfactant reverse micelles and were extracted from the micellar solution to disperse in heptane.ResultsA silver particle size in the range of 1.13 nm was observed. The UV–vis absorption spectra proposed that silver nanoparticles could be formed in the reverse micelles and relatively stabilized for at least 3 months without passivator addition. The Transmission Electron Microscope (TEM) shows that the silver nanoparticles are of spherical form and relatively uniform.ConclusionsThis process provided a simpler route for nanoparticle synthesis compared to existing systems using whole organisms or partially purified biological extracts, showing that the low-cost biosurfactant can be used for nanoparticle synthesis as a non-toxic and biodegradable stabilizing agent.     

Effect of wall permittivity on electroviscous flow through a contraction

The electroviscous flow at low Reynolds number through a two-dimensional slit contraction with electric double-layer overlap is investigated numerically for cases where the permittivity of the wall material is significant in comparison with the permittivity of the liquid. The liquid-solid interface is assumed to have uniform surface-charge density. It is demonstrated that a finite wall permittivity has a marked effect on the distribution of ions in and around the contraction, with a significant build-up of counter-ions observed at the back-step. The development length of the flow increases substantially as the wall permittivity becomes significant, meaning that the electric double-layers require a longer distance to develop within the contraction. Consequently, there is a corresponding decrease in the hydrodynamic and electro-potential resistance caused by the contraction. The effect of wall-region width on the flow characteristics is also quantified, demonstrating that the development length increases with increasing wall-region width for widths up to 5 channel widths.     

关于中蒙两国合作开拓跨国出海大通道的战略推进构想

中蒙两国共同开拓跨国出海大通道,是中国图们江区域合作开发这一国家战略的重大工程.为推动中蒙大通道工程取得进展,本文通过透析时代背景认为必须尽快开工建设"两山铁路",认证珲春作为中蒙大通道出海口的区域价值,并做出对策创意:强化政府间的战略共识,营造良好投资环境,促进东北亚区域合作,改善沿线生态环境.     

用投影法实现二维线段矩形窗口的裁剪

针对二维线段矩形窗口的裁剪提出一种新的方法—投影法。该方法思路简单,运行效率高。