紫乌头复合体nrDNA的ITS序列与系统发育分析

对乌头属 Aconitum L. 27个类群的nrDNA ITS序列进行了简约法与邻接法分析，两种方法得到的系统发育树基本一致。乌头亚属subgen. Aconitum 的蔓乌头系 ser. Volubilia 不是一个单系类群，显柱乌头系 ser. Stylosa 与兴安乌头系ser. Ambigua 各自作为单系类群也没有得到支持。特产于云南西北部横断山区的一些种之间存在非常近的系统发育关系，说明这些种可能是近期物种形成的产物。紫乌头 A. delavayi 复合体的不同类群在系统发育树上位     

乌头类藏药的分类与毒性分析

本文详述乌头类藏药与乌头类中药的分类区别，并对目前主流现代藏药本草记载的藏药乌头类植物进行了阐述，同时对乌头类藏药的毒性进行了简单分析。     

卧龙乌头的名实问题

发现卧龙乌头Aconitum wolongense W．T. Wang的模式实际上包含两种植物，即毛果甘青乌头A． tanguticum (Maxim．)Stapf．var．trichocarpum Hand.-Mazz．和展毛大渡乌头A．franchetii Fin.et Gagnep．var．villosum W．T. Wang，故将该种予以归并。     

江孜乌头化学成份

从江孜乌头植物的块根中分离得到了三个化合物。经质谱、红外光谱、紫外光谱、核磁共振氢谱、核磁共振磁谱解极及物理化学方法,证明它们分别为：“海帕乌头碱,雪乌碱和伪乌碱,其结构式分别为Ⅰ、Ⅱ、Ⅲ所示。     

国产十种乌头的染色体研究

本文描述了我国产毛茛科乌头属的10个种的染色体数目和形态。  根据该属染色体基 数为8(x＝8)，可将这些种归为二倍体、四倍体、六倍体和八倍体种。  10种乌头的染色体在数目、大小、结构上表现出相关性。其中具根状茎而多年生的种多为二倍体，染色体形态较大，第3-7号多为近端着丝点(st)染色体, 具块茎而二年生的种多为多倍体，染色体形态较小，第3-7号多为近中着丝点(sm)染色体。染色体演化方向可能是二倍体→多倍体、大→ 小、st→sm。这进一步证明根状茎种较块茎种原始。根据染色体数目和形态，又可将这些种的染色体分为两类。这进一步支持了目前分类学上的牛扁亚属和乌头亚属的划分。本文还讨论了这些种的有关分类问题。     

卧龙乌头后选模式的指定

指定了卧龙乌头Aconitum wolongense W．T．Wang的后选模式。     

中国特有的露蕊乌头亚属及铁破锣的染色体研究

The paper reports chromosomal number and chromosomal morphologies of annual Aconitum gymnandrum endemic to China and Beesia calthifolia for the first time.  Of the two spcies, chromosome number is same (X=8, 2n=16) and chromosome average lengths are 6.17μ , 10.73μ respectively.  The longest chromosome 1, the short chromosomes 3-5, 7 and the shortest chromosome 8 are metacentrical (m), the chro- mosomes 2, 6 are submetacentrical (sm), and the pairs 4, 5, 8 have satellites in the karyotype of A. gymnandrum.  In B. calthifolia, all of the chromosome 1-5 are the long m, the chromosomes 6, 8 are the short sm and the 7 is telocentrical (t). The pairs 3, 4, 6 have satellites.      According to the comparison of karyotypes of three subgenera—subgen. Para- conitum, subgen. Aconitum and subgen. Gymnaconitum in Aconitum, the evolution trend of chromosomes is further discussed.      Finally, the relationship between Aconitum and Beesia is also discussed in thispaper.     

草乌中有毒生物碱的提取分离

针对药材中乌头碱、中乌头碱、次乌头碱的提取分离方法进行了研究,本方法提取回收率与碱性苯溶液提取方法相似,方法中所用试剂成本低、毒性小,适用于乌头碱、中乌头碱、次乌头碱的提取分离以及对照品的制备。     

藏药船盔乌头的染色体数目和核型分析

首次报道藏药船盔乌头（Aconitum naviculare（Brfihi）stapf）的染色体数目和核型，研究结果表明：核型公式为2n=2x=16=4m＋8sm＋4st，不对称性核型属“2B”型。     

HPLC法测定铁棒锤中乌头碱的含量

目的建立HPLC测定铁棒锤中乌头碱的方法。方法采用高效液相色谱-紫外检测器;色谱柱为Thermo ODS-2 HYPERSIL Dim(.250mm×4.6mm,5μm);柱温为30℃;流动相为乙腈-0.3%二乙胺水溶液(35:65);流速为1.0ml/min。结果乌头碱在0.975μg-3.9μg(R=0.999)范围内线性关系良好。乌头碱加样回收率为98.06%,RSD%为1.2%,原药材中乌头碱的含量为0.1052mg/g。结论本实验建立的测定方法具有准确、简便、灵敏度高、无干扰而且重现性好的优点,适合用于铁棒锤中乌头碱含量的测定。     

中国兔儿风属(菊科)资料

The genus, Ainsliaea DC. from China is revised in this paper. Three species, A. nana Y. C. Tseng, A. pingbianensis Y. G. Tseng and A. trinervis Y. C.  Tseng, are newly described;   two species, A.  chapaensis Merr.  and A. angustifolia Hook. f. et Thoms. ex C. B. Clarke are new records for China and two  new  combinations,  A.  apteroides (Chang)  Y.  C.  Tseng  and  A. macrocephala (Mattf.) Y. C. Tseng, are made. In addition, one species, A. hypoleuca  Diels  ex  Limpr.  and  four  varieties,  A.  bonatii  Beauverd  var. arachnoidea Beauverd,  A.  pteropoda DC.  var.  leiophylla Franch.,  A.  elegans Hemsl. var. tomentosa Mattf. and A. glabra Hemsl. var.  tenuiculis (Mattf.)Chang, are reduced to synonyms.     

中国马兜铃属新分类群

 Four new species of the genus Aristolochia (Aristolochiaceae) are described as new from China.  They are Aristolochia austrochinensis C. Y. Cheng & J. S. Ma, A. caulialata C. Y. Wu,  A. salweenensis C. Y. Cheng & J. S. Ma, and A. kunmingensis C. Y. Cheng &J. S. Ma. A naturalized species, A. ringens Vahl is also reported.     

五种黄芪属植物的核型分析

The karyotypes of five species in Astragalus (A. dahuricus DC., A. mongolicus Bunge., A. adsurgens Pall., A. melilotoides Pell., A. huangheensis H. C. Fu., Y. H. Liu) were studied.  Among them, the karyotypes of A. dahuricus and A. melilotoides are re- ported for the first time.  While A. melilotoides is tetraploid (2n=4X=32), all the others are found to be diploid (2n= 2X = 16).  Based on the comparison of karyotypes, the evolu-tionary order of these 5 species is discussed.     

狭叶沙参复合体(桔梗科)的物种生物学研究

 通过对狭叶沙参复合体进行居群(共46个)采样、引种、栽培实验，生物学特性观察，染色体观察 (已发表)，杂交、同工酶比较、形态性状的定性定量分析、茎解剖和花粉形态的观察，发现：(1)该 复合体主要进行异花授粉，实生苗第二年才抽茎生花；(2)32个居群的染色体计数表明，在辽宁东部、 辽宁西部(绝大部分地区)和山西霍县的居群都为二倍体(2n=34)，而其它地方的居群为四倍体(2n 68)；(3)叶的酯酶同工酶的变异大，和一些形态特征，尤其是叶的形态和大小的变异相似，而种子的酯 酶同工酶则相当稳定；(4)通过9个居群的杂交试验，在狭叶沙参和石沙参四倍体居群间得到了一 些F1种子，而辽宁西部二倍体与上述的四倍体居群间的杂交未产生任何F1种子；(5)性状分析(包括 聚类分析与主成份分析)和茎解剖的研究，揭示了它们在叶、果实、花和种子的形态、髓纤维组织的有无、 叶锯齿和花萼裂片齿的有无和多少等形状上的变异幅度，并据此分辨出8个宗。其中辽宁西部的二倍 体宗形态上相当独特，被提升为种的等级。  其余7个宗，根据不同的分化程度被处理为三个种和5个 亚种；(6)运用杂交指数法分析了石沙参和狭叶沙参在太行山一带杂交的可能性；(7)分析讨论了复合体内各类群的起源和进化关系。      

云南剪股颖属新分类群

In the paper two species and one variety of the genus Agrostis L. are described as new from Yunnan Province, China. They are Agrostis lushuiensis,A. kunmingensis and A. myriantha Hook. f. var. yangbiensis.     

粉背蕨属一些种类的增订

Two new species of the genus Aleuritopteris (Sinopteridaceae),  A. humatifoliaX. C. Zhang et L. Shi and A. ebenipes X. C. Zhang, are described from Southern and Southwestern China. A. cremea Ching is redescribed and illustrated in the present paper. In addition, a key is also given to the species which are confused with A. cremea Ching in the genus.     

广西蜘蛛抱蛋属二新种

 Two new species of the genus Aspidistra are described from Guangxi Zhuang Autonomous Region, China. They are Aspidistra cavicola D. Fang et K.C. Yen and A.marginella D. Fang et L. Zeng.     

四川葱属二新种

 This  paper  reports two new species of Allium  L. from Sichuan, A. xiangchengense J. M. Xu and A.guanxianense J. M. Xu. The former is related to A. hookeri Thwaites, but differs from it by its lanceolate to linear-lanceolate leaves with evidently contracted base and filaments longer than the tepals, while the latter is related to A.chienchuanense J. M. Xu, but differs from it by its termi- nal scape, filaments shorter than the tepals and ovary with solitary ovule in eachlocule.