Effect of lead exposure on the immune function of lymphocytes and erythrocytes in preschool children

INTRODUCTION Over the past few years, systematic studies onthe effect of lead exposure on the human body haveled to an understanding that lead is neurotoxic andhas adverse effect on the immune system. So far,however, research on the immunotoxic effects oflead has been limited to animal experiments andpeople with occupational exposure to lead (Shen etal., 1996; Mishra et al., 2003; Liu et al., 2000).Presently, there are no reports on the influence oflead exposure on lymphocyte and …     

Effect of lead exposure on the immune function of lymphocytes and erythrocytes in preschool children

Objective: To investigate the influence of lead exposure on the immune function of lymphocytes and erythrocytes in preschool children. Materials and methods: A group of 217 children three to six years of age from a rural area were given a thorough physical examination and the concentration of lead in blood samples taken from each subject was determined. The indices of lymphocyte immunity (CD 3CD 4, CD 3CD 8, CD 4CD 8, CD-3CD 19) and erythrocyte immunity (RBC-C3b, RBC-IC, RFER, RFIR, CD35 and its average fluorescence intensity) of 40 children with blood lead levels above 0.483 μmol/L were measured and compared with a control group. Results: The blood lead levels of the 217 children ranged from 0.11 μmol/L to 2.11 μmol/L. The CD 3CD 4and CD 4CD 8 cells were lower (P＜0.01) and the CD 3CD 8 cells were higher in the lead-poisoned subjects than those in the control group (P＜0.05). CD 3 and CD-3CD 19 did not show significant differences. Although the RBC-C3b rosette forming rate was lower and the RBC-IC rosette forming rate was higher in the lead-poisoned group, this difference could not be shown to be statistically significant (P＞0.05). RFIR was found to be lower in the lead-poisoned group (P＜0.01). Compared with the control group, the positive rate of CD35 was not found to be significantly different in a group of 25 lead-poisoned children (P＞0.05), while the average fluorescence intensity was lower in the lead-poisoned group (P＜0.05). Conclusion: Lead exposure can result in impaired immune function of T lymphocytes and erythrocytes in preschool children.     

自发性高血压大鼠血管内皮细胞一氧化氮合酶的观察

目的观察高血压大鼠血管内皮细胞一氧化氮合酶在高血压不同时期的变化情况.方法采用免疫组织化学方法(ABC法)及计算机图象分析方法,观察及定量分析8周、12周、24周高血压大鼠胸主动脉及其心肌微血管内皮细胞一氧化氮合酶变化.结果在高血压不同时期胸主动脉与心肌微血管内皮细胞一氧化氮合酶含量变化基本一致12周较8周时明显增多,而24周与12周时无明显差异.结论伴随高血压病程的变化,血管内皮细胞eNOS含量发生了变化,且不同部位的变化呈正相关.     

Effect of nitrogen doping on the reduction of nitric oxide with activated carbon in the presence of oxygen

Nitrogen doping of activated carbon （AC） was performed by annealing both in ammonia and nitric oxide, and the activities of the modified carbons for NO reduction were studied in the presence of oxygen. Results show that nitrogen atoms were incorporated into the carbons, mostly in the form of pyridinic nitrogen or pyridonic nitrogen. The effect of nitrogen doping on the activities of the carbons can be ignored when oxygen is absent, but the doped carbons show desirable activities in the low temperature regime （≤500 ℃） when oxygen is present. The role of the surface nitrogen species is suggested to promote the formation of NO2 in the presence of oxygen, and NO2 can facilitate decomposition of the surface oxygen species in the low temperature regime     

Science Letters:Effect of nitrogen doping on the reduction of nitric oxide with activated carbon in the presence of oxygen

Nitrogen doping of activated carbon (AC) was performed by annealing both in ammonia and nitric oxide, and the activities of the modified carbons for NO reduction were studied in the presence of oxygen. Results show that nitrogen atoms were incorporated into the carbons, mostly in the form of pyridinic nitrogen or pyridonic nitrogen. The effect of nitrogen doping on the activities of the carbons can be ignored when oxygen is absent, but the doped carbons show desirable activities in the low temperature regime (≤500 ℃) when oxygen is present. The role of the surface nitrogen species is suggested to promote the formation of NO2 in the presence of oxygen, and NO2 can facilitate decomposition of the surface oxygen species in the low temperature regime.     

Inhibitory effect of Jeju endemic seaweeds on the production of pro-inflammatory mediators in mouse macrophage cell line RAW 264.7

Seaweed has been used in traditional cosmetics and as a herbal medicine in treatments for cough,boils,goiters,stomach ailments,and urinary diseases,and for reducing the incidence of tumors,ulcers,and headaches.Despite the fact that seaweeds are frequently used in the practice of human health,little is known about the role of seaweed in the context of inflammation.This study aimed to investigate the influence of Jeju endemic seaweed on a mouse macrophage cell line(RAW 264.7) under the stimulation of lipopolysaccharide(LPS).Ethyl acetate extracts obtained from 14 different kinds of Jeju seaweeds were screened for inhibitory effects on pro-inflammatory mediators.Our results revealed that extracts from five seaweeds,Laurencia okamurae,Grateloupia elliptica,Sargassum thun-bergii,Gloiopeltis furcata,and Hizikia fusiformis,were potent inhibitors of the production of pro-inflammatory mediators such as nitric oxide(NO),prostaglandin E2(PGE2),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).Based on these results,the anti-inflammatory effects and low cell toxicity of these seaweed extracts suggest potential thera-peutic applications in the regulation of the inflammatory response.     

Morphological and pathologic changes of experimental chronic atrophic gastritis （CAG） and the regulating mechanism of protein expression in rats

INTRODUCTION In 1998 gastrointestinal pathologists reached aconsensus on the definition of chronic atrophic gas- tritis (CAG), which was described as programmed loss of gastric gland and/or replacement by intestinal glands in gastric mucosa. CAG was recognized to be closely related with development of gastric cancer and listed as precancerous lesions in this meeting (Genta, 1998). According to Correa (1992)’s cascade of gas-tric carcinogenesis, gastric cancer was believed to develop fr…     

三七皂苷对缺血大鼠脑组织神经元型一氧化合酶的调节作用

目的:研究三七皂苷保护缺血脑组织中神经元的机制。方法:应用免疫细胞化学SP法和计算机图象分析系统观察三七皂苷(7mg.kg-1,i.m) 对不同缺血时间(4,12和24h)老龄大鼠大脑皮质、海马与尾壳核内神经元型一氧化氮合酶(nNOS)表达丰度和病理组织学变化的影响。结果:单侧结扎颈总动脉可使大脑皮层、海马与尾壳核内nNOS的表达丰度显著下降,部分神经元出现空泡样变性。预先注射三七皂苷可有效阻止大脑皮质、海马与尾壳核内nNOS表达丰度的下降,并减少这些区域的病理学改变。结论:三七皂苷可促进缺血早期脑组织表达nNOS,并由此对神经元发挥保护作用。     

Effects of chromium nanoparticle dosage on growth,body composition,serum hormones and tissue chromium in Sprague-Dawley rats

This 6-week study was conducted to evaluate the effects of seven different levels of dietary chromium (Cr) (0, 75, 150,300, 450, 600, and 1200 ppb Cr) in the form of Cr nanoparticle (CrNano) on growth, body composition, serum hormones and tissue Cr in Sprague-Dawley (SD) rats. Seventy male SD rats (average initial body weight of (83.2±4.4) g) were randomly assigned to seven dietary treatments (n=10). At the end of the trial, body composition was assessed via dual energy X-ray absorptiometry (DEXA). All rats were then sacrificed to collect samples of blood, organs and tissues for determination of serum hormones and tissue Cr contents. The results indicated that lean body mass was significantly increased (P＜0.05) due to the addition of 300 and 450 ppb Cr from CrNano. Supplementation of 150, 300, 450, and 600 ppb Cr decreased (P＜0.05) percent body fat significantly. Average daily gain was increased (P＜0.05) by addition of 75, 150, and 300 ppb Cr and feed efficiency was increased (P＜0.05) by supplementation of 75, 300, and 450 ppb Cr. Addition of 300 and 450 ppb Cr decreased (P＜0.05) the insulin level in serum greatly. Cr contents in liver and kidney were greatly increased (P＜0.05) by the addition of Cr as CrNano in the dosage of from 150 ppb to 1200 ppb. In addition, Supplementation of 300, 450, and 600 ppb Cr significantly increased (P＜0.05) Cr content in the hind leg muscle. These results suggest that supplemental CrNano has beneficial effects on growth performance and body composition, and increases tissue Cr concentration in selected muscles.     

脒霉素在人体内形成一氧化氮反应机理的量子化学研究

采用直接药物设计中的全新药物设计方法,以脒霉素为研究对象,利用量化软件Hyperchem和MOPAC6.0中的AM1方法对其在体内各种酶作用下产生一氧化氮的反应机理进行了量子化学研究,全参数优化了反应过程中反应物、产物的几何构型,通过研究此过程中各化合物的的能量、相关键长及相关净电荷的变化,讨论了这个机制的合理性。     

Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice

Objective: To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae (Danshen) in the treatment of severe acute panereatitis (SAP)- or obstructive jaundice (OJ)-induced heart injury. Methods: A total of 288 rats were used for SAP-(n=108) and O J-associated (n=180) experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups (n=12), whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups (n=15). At the corre-sponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A2 (PLA2) in blood, and pathological changes of the hearts were investigated. Results: The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly (P>0.05). The contents ofendotoxin (at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively) and PLA2 (at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively) in the treated group were significantly lower than those in the model control group (P<0.01 and P<0.001, respec-tively). Besides, myocardial pathological injuries were mitigated in SAP and OJ rats. Conclusion: In this study, we found that Salvia miltiorrhizae improved myocardial pathological changes, reduced the content of PLA2 in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats.     

Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice

Objective  To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae (Danshen) in the treatment of severe acute pancreatitis (SAP)- or obstructive jaundice (OJ)-induced heart injury. Methods  A total of 288 rats were used for SAP- (n=108) and OJ-associated (n=180) experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups (n=12), whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups (n=15). At the corresponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A₂ (PLA₂) in blood, and pathological changes of the hearts were investigated. Results  The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly (P>0.05). The contents of endotoxin (at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively) and PLA₂ (at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively) in the treated group were significantly lower than those in the model control group (P<0.01 and P<0.001, respectively). Besides, myocardial pathological injuries were mitigated in SAP and OJ rats. Conclusion  In this study, we found that Salvia miltiorrhizae improved myocardial pathological changes, reduced the content of PLA₂ in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats. Projected supported by the Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province (Nos. 2003C130 and 2004C142), the Grave Foundation Project for Technological and Development of Hangzhou City (No. 2003123B19), and the Intensive Foundation Project for Technology of Hangzhou City (No. 2004Z006), China     

复方炉甘石洗剂中氧化锌含量测定方法探索

制定以二甲酚橙（XO）为指示剂的测定复方炉甘石洗剂中ZnO含量的实验室方法,并与以铬黑T为指示剂的药典方法进行比较.其结果显示以XO为指示剂的实验方法与以铬黑T为指示剂的两种方法测定结果相比相差不大,且以XO为指示剂的实验方法具有简单,快捷,准确,滴定终点变色明显,易于观察等优点,更适合于滴定分析实验教学中复方炉甘石洗剂中ZnO含量的测定.     

Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro

Objective  

To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs).     

高效液相色谱分析西红柿中的维生素C含量

利用高效液相色谱仪对西红柿中维生素C的含量进行了分析研究。采用高效液相色谱法,以超纯水为流动相,流动相的速度为1.0ml/min,紫外检测波长为267nm,柱温在25℃的条件下,分析西红柿中维生素C的含量,及其重复性和回收率。通过线性回归具有良好的线性关系,其相关系数R2=0.9994;其回收率为98.71%~103.29%,相对标准偏差为0.79%。所建立的方法简单、快速、准确率高,适宜于西红柿中维生素C含量的测定。     

约简算法在商业银行CRM中的应用研究

针对银行CRM中的数据冗余大、数据挖掘效率低的问题,将基于属性约简的数据预处理方法应用在银行CRM中.使用决策表属性重要度属性约简算法简化客户贡献度决策表,实现决策表条件属性的归约.通过该算法在某商业银行CRM数据预处理过程中的实际应用,证实约简算法应用在银行CRM系统是有效可行的.     

龙眼果皮中多酚氧化酶活性的研究

研究龙眼果皮中多酚氧化酶(PPO)活性在不同条件下的变化规律,确定影响龙眼果实在贮藏过程中褐变的主要因素。以PPO催化邻苯二酚变色为基础,采用分光光度法研究PPO的相对活性,考察了不同pH值、温度、时间和抑制剂对酶活性的影响。结果显示,龙眼果实在贮藏初期及低pH值、低温和柠檬酸存在的条件下,PPO活性较低或受到抑制,有利于果实的保鲜。     

影响跨国公司在武汉直接投资因素实证研究

英国学邓宁认为东道国或地区的区位优势是跨国公司对外直接投资的必要条件。章以武汉这个地区作为研究对象，借鉴一般区位理论和国际直接投资相关理论的基本方法和思路来探讨影响跨国公司在武汉直接投资因素。     

贵州黑神信仰初探

贵州明清时期有较为兴盛的黑神信仰。从明代贵州巡抚王宪请祀开始,贵州黑神信仰的范围越发扩大。至清代,贵州各地已有多座供奉黑神的忠烈庙。贵州黑神信仰兴起于何时,从现有的材料已经无从考察,但是作为贵州一个较为独特的信仰,一直延续到民国时期。成为贵州一个重要的民间信仰。