共查询到20条相似文献,搜索用时 12 毫秒
1.
The anti oxidative effect of administration of 100 mg/kg bw and 200 mg/kg bw of the flower powder of Cassia auriculata (CFP) for 45 days to normoglycemic and diabetic rats (streptozotocin induced) was studied. Anti oxidative effect was not
observed in normoglycemic rats in the experiment. There was significant (P > 0.05) increase in the level of Thio Barbituric Acid Reactive Substances (TBARS), hydroperoxide and conjugated dienes and
significant (P > 0.05) decrease in the catalase, superoxide dismutase and glutathione peroxidase activities and in the level of ascorbic
acid, vitamin E and reduced glutathione in diabetic rats. The flower powder of Cassia auriculata significantly (P > 0.05) decreased the TBARS, hydroperoxide and conjugated dienes and increased the antioxidant enzymes (catalase, superoxide
dismutase and glutathione peroxidase) and non enzymic anti oxidants (ascorbic acid, vitamin E and reduced glutathione). The
antioxidatve effect of 200 mg/kg bw CFP was significantly (P > 0.05) better than 100 mg/kg bw CFP and the reference drugs (tolbutamide and metformin). The mode of action of CFP remains
to be elicited. 相似文献
2.
The objective of the present study was to evaluate hypolipidemic effect of methanolic extract of Celastrus paniculatus in experimentally induced hypercholesterolemic rats. Hypercholesterolemia was induced by feeding the animals with high fat
diet. Oral administration of methanolic seed extract (50%) of Celastrus paniculatus at the optimized dose of 65 mg/kg body weight, substantially reduced the plasma total cholesterol, triglycerides and LDL
cholesterol in comparison with induced hypercholesterolemic animal group and the results were comparable with the standard
hypocholesterolemic drug and almost similar to the control group. Atherogenic index and liver weight of treated animals also
showed significant decrease compared to the hypercholesterolemic animals. It substantially increased the HDL cholesterol level
as compared to control group. A significant increase in the activities of lipoprotein lipase and plasma LCAT enhanced hepatic
bile acid synthesis and thereby, increased degradation of cholesterol to neutral sterols. Furthermore, the activities of HMG-CoA
reductase, glucose 6-phosphate dehydrogenase and malate dehydrogenase were significantly reduced. Histological studies showed
less cholesterol deposits in the aorta of animals fed with seed extract of C. paniculatus compared to the induced hypercholesterolemic animals not given C. paniculatus supplement. 相似文献
3.
Ravi Shankar Prasad Yadav Vishal Khatri Nitin Amdare Kalyan Goswami V. B. Shivkumar Nitin Gangane Maryada Venkata Rami Reddy 《Indian journal of clinical biochemistry : IJCB》2016,31(2):203-208
Helminths are known to modulate host’s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis. 相似文献
4.
The present study was undertaken to analyze the antioxidant (both enzymic and nonenzymic) activities of leaves of Ocimum sanctum hydroalcoholic extract against cadmium induced damage in albino rats. Oral administration of cadmium as CdCl2 (6.0 mg/kg body weight) led to significant elevation of lipid peroxidation (LPO) levels and significantly decreased Superoxide
Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx), Reduced Glutathione (GSH) and Vitamin C (Ascorbate) levels.
Administration of Ocimum sanctum extract (100 mg/kg body weight, po) and (200 mg/kg body weight, po) before and after cadmium intoxication showed a significant
decrease in LPO levels and significant increase in SOD, CAT, GPx, GSH and Ascorbate levels. The results suggest that oral
administration of Ocimum sanctum extract provides significant protection against cadmium induced toxicity in Wistar albino rats. 相似文献
5.
Mahajan RS Veerpathran A Dakshinamoorthy G Sharma RD Goswami K Reddy MV 《Indian journal of clinical biochemistry : IJCB》2010,25(4):362-366
WHO-Tropical Disease Research scheme highlighted the need for development of new anti-filarial drugs. Certain antibiotics
have recently been found effective against Wolbachia, co-existing symbiotically with filarial parasites. Inflammatory response
entails oxidative mechanism to educe direct anti-microbial effect. In the present study microfilariae were maintained in vitro
in medium supplemented with varying concentrations of tetracycline, doxycycline (20–100 μg/ml) or ciprofloxacin (50–250 μg/ml)
separately to find out any involvement of oxidative mechanism in the anti-filarial effect of these antibiotics. Loss of motility
of the microfilariae was measured after 48 h and correlated with the levels of MDA, nitric oxide and protein-carbonylation.
Significant loss of microfilarial motility was recorded with increasing concentration of tetracycline and doxycycline but
with ciprofloxacin the effect was not marked. Agents with high antifilarial activity revealed significant association with
oxidative parameters in a dose dependent manner. The result suggests that oxidative effect might be exploited to design novel
antifilarial drug candidate. 相似文献
6.
Gacche RN Shaikh RU Chapole SM Jadhav AD Jadhav SG 《Indian journal of clinical biochemistry : IJCB》2011,26(3):303-308
The study was designed to evaluate the antioxidant activity and effect of Cymbopogon martinii (Roxb.) Wats. (Poaceae) leaves on the activity of monoamine oxidase and kinetics of enzyme inhibition. Ethanol extract of
C. martinii and rat brain mitochondrial monoamine oxidase preparation ware used to study the kinetics of enzyme inhibition using double
reciprocal Lineweaver–Burk plot. The DPPH was used as a source of free radical to evaluate antioxidant potential. It is observed
that, the ethanolic extract of C. martinii inhibits the monoamine oxidase activity with competitive mode of inhibition. The V
max (0.01 mM/min) remained constant while, K
m varied from 21.00 ± 1.1, 43.33 ± 1.5 and 83.33 ± 1.4 mM for 100–500 μg/ml concentration of C. martinii. The K
i values were calculated to be 90.00 ± 0.87, 75.00 ± 0.69, 68.18 ± 0.68 μg for 100–500 μg/ml concentration of C. martini. It also shows a significant DPPH (1,1-diphenyl-2-picryl hydrazine) radical scavenging (IC50 = 0.34 ± 0.05 mg/ml) and reducing activity (IC50 = 0.70 ± 0.22 mg/ml). The C. martini can be considered as a possible source of MAO inhibitor used in the treatment of depression and other neurological disorders. 相似文献
7.
This paper is an attempt to present disclosive ethics as a framework for computer and information ethics – in line with the
suggestions by Brey, but also in quite a different manner. The potential of such an approach is demonstrated through a disclosive
analysis of facial recognition systems. The paper argues that the politics of information technology is a particularly powerful
politics since information technology is an opaque technology – i.e. relatively closed to scrutiny. It presents the design
of technology as a process of closure in which design and use decisions become black-boxed and progressively enclosed in increasingly
complex socio-technical networks. It further argues for a disclosive ethics that aims to disclose the nondisclosure of politics
by claiming a place for ethics in every actual operation of power – as manifested in actual design and use decisions and practices.
It also proposes that disclosive ethics would aim to trace and disclose the intentional and emerging enclosure of politics
from the very minute technical detail through to social practices and complex social-technical networks. The paper then proceeds
to do a disclosive analysis of facial recognition systems. This analysis discloses that seemingly trivial biases in recognition
rates of FRSs can emerge as very significant political acts when these systems become used in practice.
Paper prepared for the Technology and Ethics Workshop at Twente 相似文献
8.
9.
10.
Ashley Stanley K. Chavda A. Subramanian S. V. Prabhu T. F. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2017,32(2):239-242
The dopamine receptor-D4 and the dopamine transporter have been investigated for their role in attention deficit hyperactivity disorder (ADHD) in children. Reports of their genetic association with ADHD have shown mixed results. The aim of the study was to evaluate the association of variable number tandem repeats (VNTRs) of the DRD4 and DAT1 genes with ADHD in children. A pilot 1:1 case control study, with 44 clinically confirmed ADHD cases and 44 age/gender matched healthy controls, was conducted at a tertiary care centre in Mumbai. Variable number tandem repeats of DRD4 exon 3, DAT1 intron 8 and 3′UTR were genotyped by PCR-AGE. Several allele repeats of the genes were observed in the screened subjects. Statistical significance was observed for the 10R/10R genotype of the DAT1 3′UTR VNTR between cases and controls. 相似文献
11.
12.
Aqueous root extract ofAchyranthes aspera was incorporated in the experimental diet ofLabeo rohita (rohu). Control diet was prepared without root extract. Feeding of fishes with experimental diet has significantly (p<0.05)
enhanced the serum anti-proteases level than fishes fed with control diet. 相似文献
13.
M. Halim Eshrat 《Indian journal of clinical biochemistry : IJCB》2003,18(2):54-63
InAyurvedic system of medicine in India, not only extracts of one plant or the other but also a combination of plant extracts are used
for the treatment of diabetes mellitus. The present paper reports the combined effect ofAbroma augusta andCoccinia indica known to be useful for the treatment of diabetes in Ayurveda on the fasting blood sugar, glucose tolerance and lipid profile
of Streptozotocin (STZ) induced albino rats. 300mg of water extract of the mixture of dried powdered roots ofA. augusta and leaves ofC. indica in equal proportions was given once daily for 8 weeks. After 8 weeks of treatment of Streptozotocin (STZ) diabetic rats,
the fasting blood sugar came down to almost normal value and improvement in glucose tolerance and serum lipid profile were
also observed. 相似文献
14.
T. A. Ajith 《Indian journal of clinical biochemistry : IJCB》2010,25(1):67-73
Iron is an essential nutrient for a number of cellular activities. However, excess cellular iron can be toxic by producing
reactive oxygen species (ROS) such as superoxide anion (O2−) and hydroxyl radical (HO·) that damage proteins, lipids and DNA. Mutagenic and genotoxic end products of lipid peroxidation can induce the decline
of mitochondrial respiration and are associated with various human ailments including aging, neurodegenerative disorders,
cancer etc. Zingiber officinale Roscoe (ginger) is a widely used spice around the world. The protective effect of aqueous
ethanol extract of Z. officinale against ROS-induced in vitro lipid peroxidation and DNA damage was evaluated in this study.
The lipid peroxidation was induced by hydroxyl radical generated from Fenton’s reaction in rat liver and brain homogenates
and mitochondrial fraction (isolated from rat liver). The DNA protection was evaluated using H2O2-induced changes in pBR-322 plasmid and Fenton reaction-induced DNA fragmentation in rat liver. The results indicated that
Z. officinale significantly (P<0.001) protected the lipid peroxidation in all the tissue homogenate/mitochondria. The extract
at 2 and 0.5 mg/ml could protect 92 % of the lipid peroxidation in brain homogenate and liver mitochondria respectively. The
percent inhibition of lipid peroxidation at 1mg/ml of Z. officinale in the liver homogenate was 94 %. However, the extract
could partially alleviate the DNA damage. The protective mechanism can be correlated to the radical scavenging property of
Z. officinale. The results of the study suggest the possible nutraceutical role of Z. officinale against the oxidative stress
induced human ailments. 相似文献
15.
This paper presents the results of an on-going research project aiming at developing GraviCS, an information system supporting local government and citizens in managing argumentations during the setting up of a Regional Natural Park in Southern Italy. We consider that organizational memory, structured within information systems supporting decision-making and action in organizational environments, can be a useful means for developing multilevel (individual, group, organizational) collaborative learning; in this sense the system architecture was designed referring to the organizational domain emerging in the decision making context of a Natural Park and was finalized to the dynamic representation of the organizational memory. Starting from the case study – the process for the Gravina Natural Park setting-up – , the paper describes the system's architecture and discusses some issues related to: possible dynamic representations of organizational memory; creation, use and storage of decision/learning histories. 相似文献
16.
17.
Ramesh Chander Kavita Singh A K Khanna S M Kaul Anju Puri Rashmi Saxena Gitika Bhatia Farhan Rizvi A K. Rastogi 《Indian journal of clinical biochemistry : IJCB》2004,19(2):141-148
Terminalia arjuna (T. arjuna) stem bark was successively extracted with petroleum ether (A), solvent ether (B), ethanol (C) and water (D). The lipid lowering
activity of these four fractions A, B, C, and D was evaluatedin vivo in two models viz., triton WR-1339 induced hyperlipemia in rats as well as fructose rich high fat diet (HFD) fed diabetic-
dyslipidemic hamsters. Hyperlipidemia induced by triton caused marked increase in the plasma levels of total cholesterol (Tc),
triglyceride (Tg) and phospholipids (PL) in rats. After treament withT. arjuna fractions A, B, C, and D at the doses of 250 mg/kg per oral (p.o.),only the ethanolic fraction (C) exerted significant lipid
lowering effect as assessed by reversal of plasma levels of Tc, Tg and PL in hyperlipidemic rats. In another experiment, feeding
with HFD produced marked dyslipidemia as observed by increased levels of plasma Tc, Tg, glucose (Glu), glycerol (Gly) and
free fatty acids (FFA) in hamsters. After treatment withT. arjuna fractions at the doses of 250 mg/kg p.o. only two fraction (B and C) could exert significant lowering in the plasma levels
of lipids and Glu. in dyslipidemic hamsters.In vitro experimentT. arjuna fractions at tested concentrations (50–500 μg/ml) inhibited the oxidative degradation of lipids in human low density lipoprotein
and rat liver microsomes induced by metal ions. These fractions when tested against generation of oxygen free radicals at
the concentrations (50–500 μg/ml), counteracted the formation of superoxide anions (O−2) and hydrodyl radicals (OH) in non enzymic test systems. The efficacy ofT. arjuna fractions as antidyslipidemic and antioxidant agents was found, fraction C> fraction B> fraction A. 相似文献
18.
Nidhi Sharma Veena Garg Arpita Paul 《Indian journal of clinical biochemistry : IJCB》2010,25(2):193-200
Alloxan administration in male Swiss albino mice, induced diabetes by increasing blood glucose concentration and reducing
hepatic glycogen content as compared to normal control group. Besides, serum lipid profile parameters such as total-cholesterol,
triglyceride, low-density lipoprotein and very low-density lipoprotein-cholesterol were also elevated, whereas, the level
of high-density lipoprotein-cholesterol was reduced significantly (P<0.05) in diabetic mice. Treatment of diabetic animals
with crude ethanolic extract of bark of Prosopis cineraria (P. cineraria) for 45 days, significantly lowered blood glucose
level, elevated hepatic glycogen content and maintained body weight and lipid-profile parameters towards near normal range.
Declined activity of antioxidant enzymes and concentration of non-enzymatic antioxidants were also normalized by drug treatment,
thereby reducing the oxidative damage in the tissues of diabetic animals and hence indicating the anti-diabetic and antioxidant
efficacy of the extract. 相似文献
19.
Alireza Nakhaee Mohammad Bokaeian Mohsen Saravani Ali Farhangi Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2009,24(4):419-425
In traditional medicine, Eucalyptus globulus (eucalyptus) was used for the treatment of diabetes mellitus. Hyperglycemia in
diabetes has been associated with increased formation of reactive oxygen species (ROS) and oxidative damage to tissue compounds.
The aim of this study was to evaluate the effects of eucalyptus in the diet (20 g/Kg) and drinking water (2.5 g/L) on lipid
peroxidation, protein oxidation and antioxidant power in plasma and liver homogenate, as well as glycated-Hb (HbA1C) of blood in streptozotocin-induced diabetic rats for a period of 4 weeks. Diabetes induced in rats by a single intraperitoneal
injection of streptozotocin (STZ, 65 mg/Kg). At the end of the treatment period, the level of plasma glucose, plasma and liver
malondialdehyde (MDA, the main product of lipid peroxidation), protein carbonyl (PC, one of the protein oxidation products)
and HbA1C increased and ferric reducing antioxidant power (FRAP) decreased in diabetic rats compared to normal rats. Eucalyptus administration
for 4 weeks caused a significant decrease in the plasma glucose levels, plasma and liver MDA, PC and HbA1C, also a concomitant increase in the levels of FRAP in diabetic treated rats. In conclusion, the present study showed that
eucalyptus posses antioxidant activities. Eucalyptus probably restores antioxidant power, due to the improved hyperglycemia
in streptozotocin-induced diabetic rats. 相似文献
20.
Bhetariya PJ Madan T Basir SF Varma A Usha SP 《Indian journal of clinical biochemistry : IJCB》2011,26(2):104-119
The medical, agricultural and biotechnological importance of the primitive eukaryotic microorganisms, the Fungi was recognized
way back in 1920. Among various groups of fungi, the Aspergillus species are studied in great detail using advances in genomics and proteomics to unravel biological and molecular mechanisms
in these fungi. Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus parasiticus, Aspergillus nidulans and Aspergillus terreus are some of the important species relevant to human, agricultural and biotechnological applications. The potential of Aspergillus species to produce highly diversified complex biomolecules such as multifunctional proteins (allergens, antigens, enzymes)
and polyketides is fascinating and demands greater insight into the understanding of these fungal species for application
to human health. Recently a regulator gene for secondary metabolites, LaeA has been identified. Gene mining based on LaeA
has facilitated new metabolites with antimicrobial activity such as emericellamides and antitumor activity such as terrequinone
A from A. nidulans. Immunoproteomic approach was reported for identification of few novel allergens for A. fumigatus. In this context, the review is focused on recent developments in allergens, antigens, structural and functional diversity
of the polyketide synthases that produce polyketides of pharmaceutical and biological importance. Possible antifungal drug
targets for development of effective antifungal drugs and new strategies for development of molecular diagnostics are considered. 相似文献