茶多酚抗突变和消除自由基作用的研究

采用Ｅ．ＣｏｌｉＳＯＳ显色法，小鼠骨髓细胞微核及染色体畸变试验和体外自由基消除实验等方法测定了茶多酚复合物（ＴＰ）的抗突变和消除自由基作用．结果表明：（１）ＴＰ的终浓度在２．５和５ｍｇ／ｍｌ时，对由阳性致突变物ＭＮＮＧ，ＥＭＳ和ＮａＮ＿３诱导的ＳＯＳ应答反应均具有明显的抑制作用；（２）小鼠每日ｉｇ４０、８０和１２０ｍｇ／ｋｇ剂量的ＴＰ能明显抑制由环磷酰胺（ＣＰ）诱发的骨髓ＰＣＥ的ＭＮＣＦ，三种剂量ＴＰ的抑制率分别为４０．９８％、６６．７６％和６５．９４％；（３）ＴＰ对ＭＭＣ诱发的骨髓细胞染色体畸变（ＣＡ）的抑制率分别为６２．５６％、５５．７１％和４７．９５％；（４）在Ｘ／ＸＯ－ＥＳＲ、Ｘ／ＸＯ／Ｌｕｍｉｎｏｌ－ＢＣＬ和ＩＲ／ＮＢＴ－ＳＰＭ三种模型中ＴＰ对超氧阴离子Ｏ＿２自由基均具有极强的消除作用，消除率分别为９８±６％、９８±１．２％和９５±１．５％，对Ｏ＿２的消除效率明显强于维生素Ｃ和Ｅ，对不同工艺制备的ＴＰ消除活性氧自由基作用的测定结果显示：脱咖啡碱程度高，ＥＧＣ和Ｌ－ＥＧＣＧ含量比例高的ＴＰ－３制剂消除Ｏ＿２的效率最高．因此，ＴＰ消除有害自由基，中断或终止自由基的氧化反应是抗突变，抗肿瘤作用的主要机制     

乳清酸的单扫示波极谱研究

乳清酸在０．１ｍｏｌ／ＬＨＣｌＯ４＋０．０２ｍｏｌ／ＬＮａＣｌＯ４底液中，于ＪＰ－２型示波极谱仪上，有一良好的二阶导数峰Ｅｐ＝－０．８６０±０．０１Ｖ（ＳＣＥ），浓度在１×１０－７～６×１０－５ｍｏｌ／Ｌ范围内，与峰高成线性关系，检测限为７．５×１０－８ｍｏｌ／Ｌ．实验证明，电极过程为具有催化性质的双电子可逆过程．     

甲硝唑的单扫示波极谱分析

本文系统地研究了甲硝唑在汞电极上的电极过程，证明了该电极过程为一含有吸附作用的不可逆的扩散控制过程，电子转移数为４，不可逆系数ａ＝０．７１４．建立了甲硝唑的单扫示波极谱测定法，在０．２０ｍｏｌ／Ｌ的ＮａＣｌ溶液中，于－０．７１Ｖ（ｖｓＳＣＥ）处的峰高与浓度［（０．０２─８０）ｍｇ／Ｌ］间存在良好的线性关系，检测限为２．４μｇ／Ｌ，ＲＳＤ＝２．０％（ｎ＝８），并考察了人体内常见微量物质对的影响。     

软肝丸对肝细胞再生作用的研究

本实验中采用了新药物研究中认可的ＣＣＬ４诱导大白鼠慢性肝损害的模型作为研究对象．病理结果说明使用软肝丸有良好的抗细胞坏死，能明显抑制肝纤维化过程，促进肝细胞再生作用．药效学的研究，也提示软肝丸可以降低血清ＡＬＴ及肝组织中的羟脯氨酸的含量．     

氟他胺加睾丸切除治疗晚期前列腺癌22例报告

为探讨提高晚期前列腺癌的治疗效果，回顾性研究22例晚期前列腺癌的临床资料，运用氟他胺加睾丸切除治疗前列腺癌，其中C2期8例，D1期9例，D2期5例。结果：显效17例，有效3例，死亡2例，总有效率达90．9％。结果表明氟他胺是一个单纯抗雄激素的非类固醇化合物，对前列腺组织靶细胞膜上的雄激素受体有特异性阻断作用，能抑制雄激素依赖性肿瘤细胞生长，切除睾丸能降低雄激素水平，两者产生协同作用，提高疗效。     

草鱼细胞株（ZC—7901）乌苯苷抗性突变体的筛选

采用甲基磺酸乙酯（ＥＭＳ）诱变草鱼吻端组织细胞（ＺＣ－７９０１）．用乌苯苷（Ｏｕａｂａｉｎ）筛选抗药性突变体．结果表明，ＥＭＳ对草鱼细胞的诱变效果与该药物对哺乳类细胞的诱变效果有明显差异．草鱼细胞对乌苯苷的敏感性也大大低于哺乳类细胞．ＺＣ－７９０１细胞在传代后２４ｈ，用浓度为２．８ｍｇ／ｍｌ的ＥＭＳ处理２４ｈ，诱变效果较理想，细胞杀伤率约３０％，这比通常用ＥＭＳ诱变哺乳类细胞所使用的浓度（０．４ｍｇ／ｍｌ）增大了７倍，存留下来的细胞用浓度为２５ｍｍｏｌ／Ｌ乌苯苷筛选抗性突变体，有少数细胞存活，并具有分裂增殖能力．但在细胞瓶内不易形成单层     

力平脂调血脂作用的临床观察：附68例临床分析

观察了力平脂对高脂血症者的治疗作用和安全性．确诊高脂血症者６８例，力平脂，０．１，Ｔｉｄ，服一个月为一疗程后，血清总胆固醇（ＴＣ）平均降低２０．９８％，甘油三酯平均降低５１．８８％，ＨＤＬ－Ｃ平均升高１０．２６％，ＬＤＬ－Ｃ平均降低１２．５７％，ＡｐｏＡ１平均升高２．２４％，ＡｐｏＢ平均降低２１．１０％，从调血脂的个体疗效分析，力平脂降低ＴＣ的总有效率８４．６１％，降低ＴＧ的总有效率９５．２４％，升高ＨＤＬ－Ｃ的总有效率６１．７７％，降低ＬＤＬ－Ｃ的总有效率为５５．８８％，升高ＡｐｏＡ１的总有效率３８．２４％，降低ＡｐｏＢ的总有效率７７．９４％．提示：力平脂治疗高脂血症者，对降低ＴＣ，ＴＧ，ＬＤＬ－Ｃ，ＡｐｏＢ和升高ＨＤＬ－Ｃ，ＡｐｏＡ１均有明显效果，其中降低ＴＧ的疗效尤为突出，副作用轻微．临床实践表明：力平脂是一个疗效高、安全、服用方便的调血脂新药     

大麦抗赤霉病性的遗传研究

采用特矮大麦／早熟３号、特矮大麦／紫皮大麦、特矮大麦９１－６４１１、９１－６４１１／早熟３号４具杂交组合的Ｐ１、Ｐ２、Ｆ１、Ｆ２、Ｂ１、Ｂ２世代，研究了大麦抗赤霉病性的遗传，结果表明，大麦抗郝霉病性表现 部分显性或超显性，细胞质作用不明显，在特矮大麦／早熟３号、特矮大麦／紫大麦和特矮大麦／９１－６４１１中，共同亲本特矮大感病性主要受一对隐性基因控制；在９１－６４１１／早熟３号中，抗赤霉病性为数量性     

改性沸石处理含氟水的实验研究

实验采用热处理、酸浸泡及酸热复合处理分别对天然沸石进行改性。运用X-射线粉末衍射（XRD）和热重一差热分析（TG-DTA）对改性沸石样进行了表征,发现酸浸泡将天然沸石由Ca型改性为Na型斜发沸石,热处理使沸石水脱去疏通了沸石的孔道结构。考察了各改性沸石样的除氟性能,并对其除氟机理进行了探讨。结果表明：酸热复合处理制得的改性沸石样静态除氟容量最佳,为0．294mg／g,约为天然沸石（O．032rag／g）的9倍,其动态除氟容量为0．089mg／g,除氟最佳州值为6～7,除氟容量随水样中氟离子质量浓度的增加而增加,对氟离子的吸附符合Freundlich吸附等温模式。     

一种快速检测四种毒品的测试系统

一种在８分钟内检测四种毒品的方法被开发出来．这种方法简单,快速,准确,可以一次测出尿样中是否含有吸毒者常用的四种毒品代谢物ＴＨＣ（ｌ－ｎｏｒ－Δ－９－ｔｅｔｒａｈｙｄｒｏｃａｎｎａｂｉｒｏｌ－９－ｃａｒｂｏｘｙｌｉｃａｃｉｄ）、ＣＯＣ（ｃｏｃａｉｎｅ）、ＭＯＲ（ｍｏｒｐｈｉｎｅ）、ＡＭＰ（ａｍｐｈｅｔａｍｉｎｅ）,这种测试的毒品浓度截止点分别为ＴＨＣ：５０ｎｇ／ｍｌ,ＣＯＣ：３００ｎｇ／ｍｌ,ＭＯＲ：３００ｎｇ／ｍｌＡＭＰ：１０００ｎｇ／ｍｌ．当尿样中毒品浓度在５０％和１５０％截止点浓度时,本测试方法的准确率大于９９％,尿液中可能存在会和测试试剂起交叉反应的５１种物质被证明对本测试系统无干扰作用．通过气相色谱／质谱证实是阳性的尿样经本测试系统测定,结果符合率大于或等于９７％．     

Anisotropic fluoride nanocrystals modulated by facet-specific passivation and their disordered surfaces

Rutile-type fluorides have been proven to be active components in the context of emerging antiferr-omagnetic devices. However, controlled synthesis of low-dimensional, in particular two-dimensional (2D), fluorides in a predictable and deterministic manner remains unrealized because of a lack of efficient anisotropic control, which impedes their further development in reduced dimensions. We report here that altered passivation of {110} growing facets can direct the synthesis of rutile-type fluoride nanocrystals into well-defined zero-dimensional (0D) particulates, one-dimensional (1D) rods and 2D sheets in a colloidal approach. The obtained nanocrystals show positive exchange bias and enhanced magnetic transition temperature from the coexistence of long-range antiferromagnetic order and disordered surface spins, making them strong alternatives for flexible magnetic devices and sensors.     

Emerging Trend of Mutation Profile of rpoB Gene in MDR Tuberculosis, North India

The present study was conducted on North Indian population to observe rpoB gene mutation profile in multidrug resistant Mycobacterium tuberculosis. This was an observational study. 30 cases of MDR-TB proven by culture and drug sensitivity were selected. DNA sequencing of 81 bp (codon 507–533) long RRDR of Mycobacterium tuberculosis was done to detect the sites of mutation. Out of 30 cases, 24 showed a single mutation in the RRDR region of rpoB gene in which 16 (53.33 %) showed mutation in codon 531(TCG→TTG), 5 cases (16.66 %) showed mutation in codon 526(CAC→TAC), mutation in codon 516(GAC→GTC, AAC) was present in 3 cases (10 %). It was also observed that mutation in more than one codon was present in 4 cases (13.33 %), which included deletion at codon 509(AGC→–GC), mutation at 513(CAA→CTA), 516, 526, 529(CGA→CTA) and 531. No mutation was detected in RRDR in 2 cases (6.66 %). Our finding of 13.33 % cases with multiple sites of mutation in RRDR region is in contrast to earlier studies done in North India which showed single mutation detected in RRDR of rpoB gene that highlights the emerging change in the trend of mutation profile of rpoB gene in rifampicin resistant Mycobacterium tuberculosis.     

Rapid Detection of Mutation in RRDR of <Emphasis Type="Italic">rpo B</Emphasis> Gene for Rifampicin Resistance in MDR-Pulmonary Tuberculosis by DNA Sequencing

To detect the site of mutation in RRDR of rpo B gene for rifampicin resistance in MDR-TB by DNA sequencing. 50 MDR-TB patients were enrolled in our study after informed written consent. Mycobacterial DNA was extracted from sputum samples by Universal Sample Processing (USP) method and RRDR of rpo B gene was amplified by PCR using primers RP4T and RP8T and then sequenced by automated DNA sequencing. The nucleotide sequences of RRDR of rpo B gene were compared with the reference sequence. We observed three different types of mutation in the RRDR of rpo B gene. The frequency of mutation in codon 531 (TCG → TTG), 526 (CAC → TAC) and 516 (GAC → GTC) are 60, 26.6 and 6.6% respectively. Of the total cases studied, 6.6% cases, although resistant to rifampicin, did not show any mutation in the RRDR of rpo B gene. Codon 531 (TCG → TTG) is the most common site of mutation in RRDR of rpo B gene for rifampicin resistance in MDR-pulmonary tuberculosis followed by codon 526 (CAC → TAC) and codon 516 (GAC → GTC).     

Discussion on the System of Administrative Counterpart's Right of Resistance in China

The research value of the right of resistance in the administrative law has been highlighted gradually in recent years with the enhancement of legal awareness and the increase of administrative cases in our country.In this paper,based on the concept and characteristics of the administrative counterpart’s right of resistance and the present situation of its development in our country,a conception of system of administrative counterpart’s right of resistance is proposed with the use of the regulations concerning the right of resistance in other countries for reference.     

变电站接地网的降阻措施

从分析变电站接地网电阻偏高的原因入手,对变电站接地网的降阻措施从设计方面进行了较深入的探讨,阐述了目前使用较广泛的各种降阻方法的特点及其存在的问题.     

User resistance in IT: A literature review

User resistance is a complex phenomenon long viewed as a major constraint in successful information technology implementation. User resistance, which can vary between passive and active, could be a source of guidance towards reducing problems associated with organisational change. However, rather than embracing user resistance and seeing it as a learning opportunity and a tool for managing current and future difficulties around user resistance, organisations fear it. There exist a wide literature on user resistance spanning decades; focusing separately on user resistance, and various related factors. However, there is no comprehensive overview of the research work published. This study presents a comprehensive literature review to gain a better understanding of the contents of the current user resistance literature. Based on the findings from the literature review, areas of concern and the impact of user resistance on the development of new information technology are identified and how to overcome the resistance is suggested.     

热仿真软件在电子设备设计应用中的优缺点

热仿真在目前的设计流程中大量使用,本文描述了此类软件相比传统热设计在计算扩散热阻和对流换热系数时的优点,也描述了其无法解决非线性问题和尺度差异问题的缺点。描述的同时提出了几个相关的实例进行了分析阐述。在文章的最后提出了解决这些问题可能的途径,强调了在使用这些软件的同时必须清楚掌握相关物理事实,才能有利于指导设计实践。     

多重制度压力与企业合法性倾向选择:一个理论模型

在文献研究的基础上,构建了一个多重制度压力下企业合法性倾向选择模型。针对某种特定的制度压力,只有当企业本身对该种制度压力具有抵制动机、且具有相应的抵制能力的情形下,企业才能选择抵制程度较高的合法性倾向,而在其他情形下,企业将主动或者被动地选择顺从程度较高的合法性倾向。     

乳腺癌耐受蛋白介导5-氟脲嘧啶的耐受及机制探讨

AIM To filtrate breast cancer resistance protein(BCRP)-mediated resistance agents and investigate the mechanism,so as to provide valuable datum for optimization clinical chemotherapy scheme to tumor with evaluation marker of BCRP expression. METHODS MTT assay was used to filtrate BCRP-mediated resistance agents with PA317/Tet-on/TRE-BCRP cell of different expression levels of BCRP after treated with different concentration anticancer agents. High performance liquid chromatography(HPLC) was applied to measure relative dose of intracellular retention resistance agents. Nuclear DNA fluorescence dye,Hochest 33258, staining and flow cytometry were adopted to detect apoptotic cells after treated with drugs. RESULTS There were shown increasing durg-resistance to 5-fluorouracil,methotrexate, doxirubicin, pirarubicin,etoposide and mitoxantrone followed with increasing expression of BCRP on PA317/Tet-on/TRE-BCRP cells(P＜0.05, n=3),but shown sensitive to paclitaxel, cisplatin, vincristine, mitomycin and vindesine. There also was shown significant negative correlation between the intracellular retention dose of 5-fluorouracil with different expression of BCRP(r=-0.885, P＜0.05, n=3).There were shown parallel results of that decreasing cellular apoptotic rate with increasing cellular expression of BCRP after treated with 5-fluorouracil by fluorescence dye staining and flow cytometry(P＜0.05, n=3),and also shown significate rise of the apoptotic rate of BCRP expression cells after treated with Ko143 (P＜0.05, n=3). Every group of cells could be different extently blocked in phase of G0/G1 treated with 5-fluorouracil. CONCLUSION Resistance of 5-fluorouracil could be especially mediated by conjugated with BCRP and acted as drug exclude-pump substrate. Cellular ability resistant to 5-fluorouracil-induced apoptosis could be reinforced by BCRP expression.     

Predicting mobile wallet resistance: A two-staged structural equation modeling-artificial neural network approach

The advancement in mobile technology has enabled the application of the mobile wallet or m-wallet as an innovative payment method to substitute the traditional functions of the physical wallet. However, because of pro-innovation bias, scholars have a focus on the adoption of technology and very little attention has been given to the resistance of innovation, especially in the m-wallet context. This study addressed this absence by examining the inhibitors of m-wallet innovation adoption through the lens of innovation resistance theory (IRT). By applying a sophisticated two-staged structural equation modeling-artificial neural network (SEM-ANN) approach, we successfully extended the IRT by integrating socio-demographics and perceived novelty. The study has unveiled the noncompensatory and nonlinear relationships between the predictors and m-wallet resistance. Significant predictors from SEM analysis were taken as the ANN model’s input neurons. According to the normalized importance obtained from the multilayer perceptrons of the feed-forward-back-propagation ANN algorithm, we found significant effects of education, income, usage barrier, risk barrier, value barrier, tradition barrier, and perceived novelty on m-wallet innovation resistance. The ANN model can predict m-wallet innovation resistance with an accuracy of 76.4 %. We also discussed several new and useful theoretical and practical implications for reducing m-wallet innovation resistance among consumers.