Iodogen法碘化标记单克隆抗体通用技术研究

报道了Ｉｏｄｏｇｅｎ法碘化标记单克隆抗体（单抗）的最佳条件和对标记单抗免疫活性影响的实验结果，结果表明，当Ｉｏｄｏｇｅｎ：ＭｃＡｂ＝１：４缓冲液ｐＨ＝７．４，旋涡搅拌１０ｍｉｎ时，标记率、放化纯和峰值百分率均较高，标记单抗的免疫活性与标记前比较没有统计学差异（Ｐ＞０．０５）。￣１３１Ｉ－ＨＣＭ８３和￣１２５Ｉ－ＶＣＭＣ与阳性对照结合率为３８．２％和３０．１％，Ｐ／Ｎ为９．１和３０．１。此通用技术具有推广应用价值。     

CT扫描对腮腺恶性肿瘤TNM分期的价值探讨

根据３６例腮腺恶性肿瘤临床资料，评价了ＣＴ扫描对腮腺恶性肿瘤ＴＮＭ分期的价值，并详细分析了ＣＴ扫描对准确测量原发灶大小及判断艰苦 巴结有无肿瘤转移的情况，着重讨论了颈淋巴转移的ＣＴ诊断标准。与术后病理结果相对照，得出触诊法对颈淋巴转移诊断准确率为６９．４％，ＣＴ扫描的诊断准确率为８０．６％。     

HPLC法测定参皇软膏中人参皂苷Rg1的含量

探讨参皇软膏中人参皂苷Rg1含量的另一种测定方法。方法：采用HPLC法，色谱柱为Diamonsil　C18，乙腈-0.05％磷酸溶液（100：400）为流动相。检测波长为203nm，流速1．0ml／min，以外标法按峰面积计算。结果：人参皂苷Rg1进样量在0．1253ug-0．7518ug范围内与峰面积线性关系良好（r=0．9999），该法重现性好与薄层扫描法有很好的规律对应。结论：本法与薄层扫描比较有准确、检验周期短的优点，可替代现有的薄层扫描法。     

乳清酸的单扫示波极谱研究

乳清酸在０．１ｍｏｌ／ＬＨＣｌＯ４＋０．０２ｍｏｌ／ＬＮａＣｌＯ４底液中，于ＪＰ－２型示波极谱仪上，有一良好的二阶导数峰Ｅｐ＝－０．８６０±０．０１Ｖ（ＳＣＥ），浓度在１×１０－７～６×１０－５ｍｏｌ／Ｌ范围内，与峰高成线性关系，检测限为７．５×１０－８ｍｏｌ／Ｌ．实验证明，电极过程为具有催化性质的双电子可逆过程．     

头颈癌患者手术前后T细胞亚群的观察

对３０例头颈癌患者手术前后的Ｔ淋巴细胞亚群变化进行观察，结果表明：头颈患者的ＣＤ＋８较对照组明显升高（Ｐ＜０．０１），ＣＤ＋４／ＣＤ＋８值比健康人显著降低（Ｐ＜０．０１），且有随临床分期增加而ＣＤ＋８升高及ＣＤ＋４／ＣＤ＋８比值下降的趋势，Ⅰ－Ⅱ期与Ⅲ－Ⅳ期相比有显著性差异（Ｐ＜０．０１）；而手术后ＣＤ＋８逐渐下降，ＣＤ＋４／ＣＤ＋８值却较术前有明显回升，术前与术后比较有显著性差异（Ｐ＜０．０１）．说明头颈癌患者存在着明显的细胞免疫功能紊乱，并随病情的发展而加重；而彻底切除肿瘤是迅速恢复机体细胞免疫功能的有效手段．提示：Ｔ淋巴细胞亚群检测对头颈癌患者的病情发展、免疫功能评定及疗效观察具有一定参考价值     

一种快速检测四种毒品的测试系统

一种在８分钟内检测四种毒品的方法被开发出来．这种方法简单,快速,准确,可以一次测出尿样中是否含有吸毒者常用的四种毒品代谢物ＴＨＣ（ｌ－ｎｏｒ－Δ－９－ｔｅｔｒａｈｙｄｒｏｃａｎｎａｂｉｒｏｌ－９－ｃａｒｂｏｘｙｌｉｃａｃｉｄ）、ＣＯＣ（ｃｏｃａｉｎｅ）、ＭＯＲ（ｍｏｒｐｈｉｎｅ）、ＡＭＰ（ａｍｐｈｅｔａｍｉｎｅ）,这种测试的毒品浓度截止点分别为ＴＨＣ：５０ｎｇ／ｍｌ,ＣＯＣ：３００ｎｇ／ｍｌ,ＭＯＲ：３００ｎｇ／ｍｌＡＭＰ：１０００ｎｇ／ｍｌ．当尿样中毒品浓度在５０％和１５０％截止点浓度时,本测试方法的准确率大于９９％,尿液中可能存在会和测试试剂起交叉反应的５１种物质被证明对本测试系统无干扰作用．通过气相色谱／质谱证实是阳性的尿样经本测试系统测定,结果符合率大于或等于９７％．     

108例病毒性肝炎患者血清补体的研究

报告１０８例病毒性肝炎患者血清总补体（ＣＨ＿（５０））和Ｃ＿３值的检测结果：急性肝炎的ＣＨ＿（５０）为６８．４±６．１３单位／ｍｌ，Ｃ＿３为１３０．２±６．６７ｍｇ％，均高于正常组（ＣＨ＿（５０）为４８．５±５．７５单位／ｍｌ，Ｃ＿３为１０２±５ｍｇ％），Ｐ＜０．０１；其他类型的肝炎患者组的补体均低于正常组，Ｐ＜０．０１．补体的含量与Ｔ细胞花环（Ｅｔ花环）呈中度相关，与ＧＰＴ呈高度相关，因此测定ＣＨ＿（５０）和Ｃ＿３可作为衡量患者机体免疫功能和判断病毒性肝炎预后的指标之一。     

人补体C4的RFLP与HLA分型之间关系的探讨

应用Ｓｏｕｔｈｅｒｎ印迹杂交法、高压琼脂糖凝胶电泳和标准的微量淋巴细胞毒试验对中国汉族人４４例正常无相关个体进行了Ｃ４基因限制性片段长度多态性（ＲＦＬＰ）、Ｃ４蛋白分型及ＨＬＡ－ＤＲ分型的测定，结果发现Ｃ４ＲＦＬＰ Ａ、Ｂ二种格局在Ｃ４Ｂ＊Ｑ０的表现型上有显著差异（Ｐ〈０．０５）；ＲＦＬＰ Ａ型格局与ＤＲ４相关（Ｐ〈０．０５）。     

高原肺水肿肺泡灌洗液中体免疫物质的含量变化及其意义

对８例高原肺水肿肺泡灌洗液听的免疫球蛋白，补体，组胺与５－羟色胺含量进行了检测，并与８例移居西藏高原５ｄ的健康成人对比，高原肺水肿患者的肺泡灌洗液中ＩｇＧ，ＩｇＭ，Ｃ３，Ｃ４与组胺含量均显著增高（Ｐ〈０．０１），并见Ｃ５，Ｃ９增高（Ｐ〈０．０５）。高原肺水肿的发生与体液免疫反应密切相关，高原肺水肿可能为超敏反Ⅲ型疾病的局部表现。     

痉挛性脑瘫患儿的脑电图和脑CT分析探讨

目的分析探索痉挛性脑瘫患儿的诊断与脑电图、头颅CT形态学改变的关系。方法研究对象人民医院对131例痉挛性脑瘫患儿,采用国际10／20系统电极进行脑电图检查,并用螺旋CT常规颅脑扫描,对比两种扫描结果。结果131例患儿中脑电图异常83例（异常率63．4％）,CT异常104例（异常率79．4％）,痉挛型脑瘫患儿的脑CT异常率高于脑电图异常率,差异有显著性（P〈0．05）。结论脑电图和脑CT都是小儿脑性瘫痪中不可或缺的检查手段,两者结合具有互相补充的作用,对脑瘫的诊断及预后判断具有重要参考意义。     

20例胰腺囊性肿瘤的诊断和外科治疗

目的:探讨胰腺囊性肿瘤的诊断和治疗.方法:对2003年1月～2008年8月诊治的20例胰腺囊性肿瘤进行治疗方法及效果回顾性分析.结果:本病临床表现无特征性.B超和CT捡查能发现囊肿但不能确定类型.胰腺肿块为囊性、囊实性或不规则分叶状.胰腺囊腺瘤12例均完整切除,术后无复发.胰腺囊腺癌8例,6例完整切除,3年生存率66%.结论: B超和CT是诊断胰腺囊性肿瘤的有效方法.手术切除是唯一的治疗方法.     

主动脉夹层31例临床回顾性分析

目的:探讨主动脉夹层患者的临床特点及其与Stanford分型、临床症状的关系,减少误诊率。方法:回顾性分析广西中医药大学附属瑞康医院2005年1月至2015年9月间收治确诊为主动脉夹层的住院治疗患者31例的临床资料、生化指标及转归。按Stanford分型及性别分组,对每组患者进行统计分析。结果:31例患者中男性患者28例,女性3例,既往有高血压病史15例,缺血性心肌病7例,马方综合征1例。5例死亡(2例死于并发症,3例自然破裂),死亡率16.12%。3例行人工血管置换,2例行带膜支架植入成功,男性发病年龄小于女性,二者差异有统计学意义(P=0.043)。女性患病人数明显少于男性。男女组患Stanford A、B型年龄差异无统计学意义;A型住院天数短于B型,但差异无统计学意义(P=0.053);A型转归差于B型;合并高血压患者入院收缩压高于无高血压患者,舒张压二组无差别。结论:在入院治疗患者中,男性患病人数较多。Stanford A型住院天数较短,预后较B型差。     

胸腹内脏器病变的CT/US导引经皮细针穿刺活检的临床研究

目的应用和比较不同穿刺针、不同导引技术对穿刺活检结果的影响。材料和方法90例中男性63例,女性27例,平均年龄54岁(23-78岁),胸部66例,腹部24例;按穿刺针不同分DFBN(dispos able fanseen biopsy needle)和ADGSTN(automatic disposable guillotine soft tissue needle)两组;比较两组的诊断正确率、并发症发生情况。结果所有病例均穿刺成功并获得细胞学和/或组织学材料,细胞学检查正确者9/25例(36%),组织学检查正确者66/91例(72.53%),DFBN和ADGSTN两种穿刺针在标本的大小和完整性、病理学结果、诊断正确率(55.81%/91.30%)有差异(P<0.01),穿刺活检在肿瘤患者中的诊断正确率为78.95%,发生并发症8例(8.89%),其中气胸2例,咯血或痰中带血6例。结论CT/US 导引经皮细针穿刺活检是一项先进的影像学与病理学活检相结合的技术,对于胸腹内不明原因的占位性病变,尤其是怀疑肿瘤者是一项简便易行、较安全的诊断手段。ADGSTN较DFBN材料满意且诊断正确率高。     

Unusually high serum CA19.9 in gastric carcinoma: A case report

We describe a case of poorly differentiated adenocarcinoma of stomach, which did not present typical symptoms of gastrointestinal malignancy on first visit. The patient, a 62 year old smoker presented with shortness of breathe and pain in right lumbar region with no history of fever. Bone scans revealed multiple hot spots in skull, sternum, lumbar vertebrae and both iliac crests. A series of tumor markers were ordered which include PSA, CEA, CA19.9, CA 72.4 and AFP. Serum PSA and AFP concentrations were within normal range. Serum CEA and CA 72.4 were raised significantly. Markedly elevated levels of serum CA19.9 were found (>45000 U/ml) in this patient. CT chest and bronchoscopic examination ruled out the possibility of cancer lung. Upper GI tract endoscopy was done to find out lesion in GI tract. An ulcerative lesion was found in lesser curvature of stomach. Histopathological examination of endoscopic biopsy revealed a poorly differentiated adenocarcinoma of stomach. An unusually high serum CA19.9 (>45000U/ml) in case of gastric carcinoma has not been reported earlier.     

MTHFR (Ala 222 Val) polymorphism and AMI in patients with type II diabetes mellitus

The prevalent Ala222Val single nucleotide polymorphism of the MTHFR gene has been shown to be associated with type II diabetes. The objective of the present study was to find out whether there is genetic predisposition for development of acute myocardial infarction in type II diabetes mellitus among South Indian Tamil population. PCR-based restriction enzyme analysis was performed in DNA isolated from 120 acute myocardial infarction patients with diabetes mellitus and 100 non diabetic healthy individuals with no documented cardiovascular diseases. The results indicate that the MTHFR 677TT genotype is absent in both case and controls. The MTHFR 677CT genotype was observed among 32 (26.7 %) cases and 20 (20%) controls and the MTHFR 677CC genotype among 88 (73.3%) cases and 80 (80%) controls. The allelic frequencies were in accordance to Hardy Weinberg equilibrium. There was no statistical difference in genotype distribution between cases and controls. In conclusion, we suggest that the analysis of MTHFR genotyping for C677T polymorphism alone need not be considered to find out whether there is genetic predisposition for development of acute myocardial infarction in type II diabetes mellitus among South Indian Tamil population.     

三碘苯酚乙基纤维素微球的制备

为提高造影剂的颗粒度,减少血池造影剂透过血管壁向组织间质渗出,影响血池造影剂的造影效果,采用液中干燥法( in-liquid drying method)制备了三碘苯酚乙基纤维素微球，研究了三碘苯酚与乙基纤维素的比例对微球成球性，粒径以及载药量的影响，发现两者的比例为1：2时成球性最好，所得微球为规整的球形，微球粒径最小，平均粒径D50为3.68um, 载药量为41.4%。     

经纤维支气管镜防污染毛刷采样诊断恶性肿瘤继发肺部真菌感染26例分析

由于化疗、放疗、免疫抑制剂治疗、介入治疗的广泛开展及抗生素的广泛应用,恶性肿瘤继发肺部真菌感染呈上升趋势。肺部真菌感染的确诊有赖于真菌的培养和分离,而常用的咳痰标本又极易受到污染,很难确定其病原性。我们经纤维支气管镜(纤支镜)防污染毛刷(protectedspecimenbrush,PSB)对135例可疑肺部真菌感染患者采样进行真菌培养和涂片检查,其中56例恶性肿瘤继发的肺部感染患者中26例诊断为肺部真菌感染。现报告如下。1,资料与方法1.1,临床资料56例均为我院住院和门诊可疑肿瘤继发的肺部真菌感染患者,男31例,女25例,年龄47￣68岁,平均年龄6…     

Crucial control measures to contain China's first Delta variant outbreak

The SARS-CoV-2 B.1.617.2 (Delta) variant flared up in late May in Guangzhou, China. Transmission characteristics of Delta variant were analysed for 153 confirmed cases and two complete transmission chains with seven generations were fully presented. A rapid transmission occurred in five generations within 10 days. The basic reproduction number (R₀) was 3.60 (95% confidence interval: 2.50–5.30). After redefining the concept of close contact, the proportion of confirmed cases discovered from close contacts increased from 43% to 100%. With the usage of a yellow health code, the potential exposed individuals were self-motivated to take a nucleic acid test and regained public access with a negative testing result. Facing the massive requirement of screening, novel facilities like makeshift inflatable laboratories were promptly set up as a vital supplement and 17 cases were found, with 1 pre-symptomatic. The dynamic adjustment of these three interventions resulted in the decline of Rt from 5.00 to 1.00 within 9 days. By breaking the transmission chain and eliminating the transmission source through extending the scope of the close-contact tracing, health-code usage and mass testing, the Guangzhou Delta epidemic was effectively contained.     

Strong Association of C677T Polymorphism of Methylenetetrahydrofolate Reductase Gene With Nosyndromic Cleft Lip/Palate (nsCL/P)

Methylenetetrahydrofolate reductase (MTHFR) is essential for DNA biosynthesis and the epigentic process of DNA methylation. It has been reported that abnormal DNA methylation contributes to the pathogenesis of congenital anomalies. There were many published case control studies assessing the associations of MTHFR C677T polymorphism with risks of nosyndromic cleft lip with and without palate (nsCL/P), but with inconsistent results. To derive a more precise estimation of the relationship, a meta-analysis was performed. Eligible articles were identified by search of databases including PubMed, Science Direct, Google Scholar and Springer Link up to December, 2015. Finally, a total of 22 studies with 3724 nsCL/P cases and 5275 controls were included in the present meta-analysis. Odds ratios (ORs) with corresponding 95% confidence intervals (95% CIs) were pooled to assess the association. Subgroup analysis based on ethnicity was also performed. All statistical analyses were done by MIX program. Meta-analysis results suggested that MTHFR C677T polymorphism contributed to the increased nsCL/P risk in overall population using four genetic models except homozygote model (for T vs. C: OR = 1.24, 95% CI = 1.1–1.4; for TT + CT vs. CC: OR = 1.29, 95% CI = 1.04–1.59; for CT vs. CC: OR = 1.26, 95% CI = 0.98–1.63; for TT vs. CC: OR = 1.02, 95% CI = 0.74–1.4; for TT vs. CT + CC: OR = 1.36, 95% CI = 1.05–1.74). In conclusion, results of present meta-analysis suggested that MTHFR C677T polymorphism is significantly associated with nonsyndromic orofacial cleft.