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1.
Dil-Afroze Dinesh Sharma G. N. Dhobi Sonaullah Shah Rafiqa Eachkoti Ishraq Hussain Zafar A. Shah Mushtaq A. Siddiqi 《Indian journal of clinical biochemistry : IJCB》2006,21(2):76-79
Pleural effusion is one of the commonest presentations of tuberculosis, the clinical manifestations being typically abrupt
resembling bacterial pneumonia. Since delayed hypersensitivity is the underlying immune response, bacterial load is very low.
Owing to these facts, tuberculous pleurisy as an extra-pulmonary disease poses a diagnostic dilemma. The conventional bacteriological
methods rarely detect Mycobacterium tuberculosis in pleural fluid and are of limited use in diagnosis of tuberculous pleurisy.
We evaluated the efficacy of polymerase chain reaction (PCR) in the diagnosis of tuberculous pleurisy by targeting the gene
segment coding for MPB64 protein specific forMycobacterium tuberculosis. Based on the clinical criteria, 82 patients with lymphocytic exudative pleural effusion were included in the study. Patients
were analyzed in two groups; one group consisting of 48 patients of tubercular pleural effusion confimed by various diagnostic
procedures and another group of 34 patients comprising of non-tubercular pleural effusion. There were no false positive results
by PCR and the specificity worked out to be 100%. Twenty two patients tested positive for Mantoux with a sensitivity of 45%.
ZN-staining for AFB was found in samples from 15 patients (20% sensitivity). ADA was positive for 28 patients with a sensitivity
of 53%. PCR was positive for 32/48 patients (67% sensitivity). Thus, PCR was found to be more sensitive than any other conventional
method in diagnosis of clinically suspected tubercular pleurisy. 相似文献
2.
Senthilkumar Kamatchiammal Dhashinamoorthy Saravanakumar Nagalingeswaran Kumarasamy Sunithi Solomon Manjula Sritharan Venkataraman Sritharan 《Indian journal of clinical biochemistry : IJCB》2000,15(2):76-82
We have developed a simple, economical and reproducible method for processing blood samples from HIV infected patients for
diagnosis of tuberculosis. The procedure was validated on 55 samples selected for tuberculosis based on clinical criteria.
52 patients had radiological changes indicative of pulmonary tuberculosis of which only 28 were positive for AFB in sputum
(sensitivity 54%) and 27 for tuberculin (sensitivity 52%). 26 HIV positive patients who showed positive X-ray did not react
to tuberculin. The genus PCR probe missed 3 samples (sensitivity 94%) compared to X-ray.M.tuberculosis was detected in the blood of all X-ray positive cases by PCR using TB400 probe (sensitivity 100%) and another probe forM. tuberculosis, IS6110, missed 6 of them (sensitivity 88% compared to X-ray and 89% compared to TB400). It is proposed that this simple
sample processing method could be used to screen all blood samples quickly for mycobacteremia using the genus PCR and only
those positive for mycobacteria need to be tested forM.tuberculosis. This would save the scarce resources and time by reducing significantly the number of samples to be screened for species
confirmation. 相似文献
3.
Syed Mazher Husain M. P. J. S. Anandaraj 《Indian journal of clinical biochemistry : IJCB》1995,10(2):122-125
Hemoglobin E (beta-26Glutamic acid→Lysine) is the second most prevalent hemoglobin variant in the world. 293 blood samples from cases referred from several hospitals in the region of Andhra Pradesh were screened for the detection of hemoglobinopathies. Four samples were found to be in heterozygous state for Hb E condition. Mutation in two of these heterozygotes was analysed using a 722 base pair (bp) amplified DNA fragment from beta-globin gene and restriction enzyme Mnl 1. A 232bp DNA fragment was found to be associated with the Hb E mutation. 相似文献