Spatial memory in rats: Resistance to retroactive interference

Treatments that interfere with animals’ short-term retention (e.g., in delayed matching-to-sample) were studied using a spatial memory task. Rats performed in an eight-arm radial maze in which choosing each arm without repetition was the optimal behavior. Performances were interrupted between fourth and fifth choices for a delay of 15 sec to 2 min. A variety of events occurring during the delay interval did not disrupt memories for prior choices (as assessed by the accuracy of postdelay choices). The ineffective treatments included variations in visual and auditory environments, removal from the maze, food consumed during the delay, a distinctive odor added to the maze, or combinations of these manipulations. Additionally, performance on another spatial task (a four-arm maze) during the delay between Choices 4 and 5 did not interfere with performance in the eight-arm maze. These findings suggest that rats’ memories for spatial locations are immune to retroactive interference, at least within the range of conditions reported, and that the rat can successfully segregate memories for spatial locations established in different contexts.     

Reactivation of an appetitive discrimination memory following retroactive interference

The present experiment employed an appetitive spatial discrimination response to examine alleviation of forgetting induced by retroactive interference. For separate groups of rats, a footshock that had occurred during acquisition contingent upon a correct response was tested for its effectiveness as a reactivation treatment. The results indicated that the footshock effectively alleviated forgetting, but only for rats experiencing the shock stimulus during original learning. These results suggest that a reactivation treatment may arouse affective or stimulus properties in common with the target memory to elicit retrieval of that memory.     

Codes and coding processes in pigeon short-term memory

The delayed matching-to-sample (DMTS) task was used in three experiments to investigate how pigeons code information about sample stimuli. In all experiments, each trial consisted of a signaled presentation of a sample stimulus for a fixed duration followed, after some delay, by the presentation of three comparison stimuli. After incorrect first choices, the bird was allowed a second choice between the remaining two stimuli. It was found, in Experiment 1, that the probability of a second choice error declined with increasing sample duration. This result is consistent with a gradual short-term memory encoding process but not with a simple two-state all-or-none process. In the second experiment, it was found that the distribution of first-choice errors was affected by the particular sample occurring on a trial. This result is inconsistent with a two-stage discrete state memory/attention model based on the assumption that encoding of the sample and attention to the comparison stimuli are both independent all-or-none processes. The third experiment involved symbolic DMTS, in which the sample stimuli varied along a dimension different from that along which the comparison stimuli varied. With increasing delay between sample and comparison stimulus presentations, the pigeons were more likely to confuse test stimuli than to confuse sample stimuli. The results of these experiments lead to the conclusion that pigeon DMTS performance depends on a gradual encoding process in which a representation isomorphic with the test stimuli is generated and maintained.     

Timing in retroactive interference

Retroactive interference is conventionally viewed as attenuated retrieval of a target association due to the training of a second association between training and testing of the target association. In three experiments in which water-deprived rats were used as subjects, we manipulated the durations of the time between cue termination and outcome onset (Experiment 1), the durations of the target and the interfering cues (Experiment 2), and the durations of the outcome used during target and interfering training (Experiment 3). Greater interference was consistently observed between associations bearing a high degree of similarity in their temporal structure, which suggests that interference occurs between complex representations that encode not only the physical attributes of the stimuli, but also their temporal characteristics.     

Effects of signaled retention intervals on pigeon short-term memory

In three delayed matching-to-sample experiments, pigeons were given distinctive stimuli that were either correlated or uncorrelated with the scheduled retention intervals. Experiment 1 employed a single-key, go/no-go matching procedure with colors as the sample and test stimuli; lines of differing orientations signaled short or long delays for one group, whereas the lines and the delays were uncorrelated for the other group. The function relating discriminative test performance to delay length was steeper in the correlated group than in the uncorrelated group. In addition, the line orientation stimuli controlled differential rates of sample responding in the correlated group, but not in the uncorrelated group. In Experiment 2, subjects extensively trained with correlated line orientations were exposed to reversed cues on probe trials. Miscuing decreased discriminative test responding at the short delay, but enhanced it at the long delay. As in the correlated group of the first experiment, rates of sample keypecking were higher in the presence of the “short” time tag than in the presence of the ”long” time tag. Experiment 3 used a three-key choice-matching procedure and a within-subjects design, and equated reinforcement rate at the short and long delays. When auditory stimuli were correlated with delay length, the function relating choice accuracy to delay was steeper than when the stimuli and the delays were uncorrelated. The consistent effects of signaled retention intervals on memory performance may be understood in terms of differential attention to the sample stimuli.     

Rat spatial memory: Resistance to retroactive interference at long retention intervals

An attempt was made to disrupt memory for spatial information by interpolating a task of high similarity to the to-be-remembered task during a long retention interval. Rats were trained in an 8-arm maze in which choosing each arm without repetition was the optional strategy. A 4-h delay was imposed between the 4th and 5th choices. At various times during the retention interval, the rats ran a second identical maze located in another room. No evidence of retroactive interference was observed. In the second experiment, the rat was required to remember the interpolated spatial task during the retention test. This was accomplished by allowing the rat to make four choices in the first maze and then, after a variable period of time, four choices in the second maze. Four hours after exposure to each maze, retention was tested. Choice accuracy on the retention tests was high and equivalent on both mazes. Requiring the rat to remember which arms it had visited in a second maze did not impair memory for the first maze. These results demonstrate that rats can segregate spatial memories established in different contexts with considerable proficiency.     

Representation strength in pigeon short-term memory: Effect of delay training

An attempt was madeto manipulate the strength of internal stimulus representations by exposing pigeons to brief delays between sample offset and comparison onset in a delayed conditional discrimination. In Experiment 1, pigeons were first trained on delayed conditional discrimination with either short (0.5-sec) delays or no delays. When delays were increased by 2.0 sec, birds trained with a delay performed at a higher level than did birds trained with no delays. In Experiment 2, subjects were first trained on a delayed simple discrimination. Following a circle stimulus, responses to a white key were reinforced; however, following a dot stimulus, responses to the white key were not reinforced. The pigeons were then trained on a delayed conditional discrimination involving hue samples and line-orientation comparisons with differential outcomes. Choice of vertical following red yielded food; choice of horizontal following green yielded no food. Mixed delays were then introduced to birds in Group Delay, whereas birds in the control group received overtraining. When tested on a delayed simple discrimination with hue stimuli (red and green initial stimuli followed by white response stimulus), pigeons in Group Delay tended to perform at a higher level than did birds in the control group (i.e., although the birds in both groups responded more following red than following green, birds in Group Delay did this to a greater extent than did birds in the control group). Thus, experience with delays appears to strengthen stimulus representations established during training.     

Retrospective revaluation of associative retroactive cue interference

Two fear-conditioning experiments with rats assessed whether retrospective revaluation, which has been observed in cue competition (i.e., when compounded cues are followed with an outcome), can also be observed in retroactive cue interference (i.e., when different cues are reinforced in separate phases with the same outcome). Experiment 1 found that after inducing retroactive cue interference (i.e., X-outcome followed by A-outcome), nonreinforced presentations of the interfering cue (A) decreases interference with responding to the target cue (X), just as has been observed in retrospective revaluation experiments in cue competition. Using the opposite manipulation (i.e., adding reinforced presentations of A), Experiment 2 demonstrated that after inducing retroactive cue interference, additional reinforced presentations of the interfering cue (A) increases interference with responding to the target cue (X); alternatively stated, the amount of interference increases with the amount of training with the interfering cue. Thus, both types of retrospective revaluation occur in retroactive cue competition. The results are discussed in terms of the possibility that similar associative mechanisms underlie cue competition and cue interference.     

Differential effects of omitting comparison stimuli on symbolic and identity matching to sample: Evidence for altered instructional processes in pigeon short-term memory

Two experiments were performed to determine the effects of omitting the comparison stimuli in a matching-to-sample task. In Experiment 1, birds were trained initially on both symbolic and identity matching to sample. Comparison stimuli were then omitted following the presentation of a particular sample stimulus, and this decreased the number of sample (observing) responses. The reintroduction of the comparison stimuli on subsequent probe trials revealed that the accuracy of symbolic matching was reduced to chance levels, while identity matching accuracy was significantly below chance. In Experiment 2, a similar procedure was employed; however, observing responses to the comparison-omitted samples were maintained by direct reinforcement (fixed ratio 20). Matching accuracy during probe trials was again at chance levels for symbolic matching but, contrary to Experiment 1, was significantly above chance for identity matching. The differential effects of omitting comparison stimuli on symbolic and identity matching trials in these two experiments were interpreted within a framework which assumes that instructional processes are altered by comparison-omission procedures.     

Pigeons’ short-term memory for temporal and visual stimuli in delayed matching-to-sample

In the present experiment, we compared directly pigeons’ short-term memory of temporal and visual stimuli in a delayed matching-to-sample task. The sample stimuli consisted of red and green lights presented for 5 and 30 sec, followed by a retention interval and blue and yellow comparisons. For subjects in the visual group, duration was irrelevant and the color of the sample was the conditional cue. For animals in the temporal group, color was irrelevant and duration of the sample was the conditional stimulus. The results showed that acquisition of the matching task was faster and accuracy was higher in the visual than in the temporal group. More importantly, memory of either sample generally declined at a similar rate when the duration of the retention interval was increased and when the intertrial interval was reduced. Taken together, the results indicate that with 1–8-sec retention intervals, short-term memory for temporal stimuli is similar to that found with color-visual samples. The findings are discussed in terms of retrospective and prospective processing.     

Reading ability and short-term memory: The role of phonological processing

The aim of the current study was to further explore the connection between verbal short-term recall and phonological processing for two purposes: (a) To investigate the basis of short-term memory deficits for children with reading disability, and (b) To further explore the origin of developmental verbal memory span increases.Using a variety of memory and phonological tasks, reading group comparisons were conducted testing third-grade good readers and poor readers, and developmental changes were studied with pre-kindergarten, first-grade and third-grade children. The main finding was that a strong relationship was observed between efficiency of phonological processes and capacity of verbal memory supporting the hypothesis that reducing phonological processing requirements in verbal short-term memory increases available resources for storage. No such relationship was found between phonological processing and nonverbal memory. This conclusion was supported by two findings: (a) The verbal short-term memory deficits in poor readers significantly correspond with less accurate phonological processing, and (b) Developmental increases in verbal STM are accompanied by more accurate and rapid execution of phonological tasks.     

Biases in pigeon working memory

The psychophysics and short-term retention of pigeons’ responses to rate of stimulus change were assessed in two experiments, using a symbolic delayed matching-to-sample procedure. In Experiment 1, the birds discriminated between steady and flickering lights. Psychophysical assessments of the discrimination suggested a mix of analogical and categorical strategies. Retention tests revealed a consistent bias to respond to the choice associated with theflickering sample. In Experiment 2, the birds discriminated between different rates of stimulus change (slow vs.fast). Retention tests indicated a bias to respond to the alternative associated with theslow sample. Transfer tests to new stimulus values revealed that the birds processed the stimuli in an absolute, rather than a relational fashion. These findings are related to recent work on biased forgetting in animal working memory experiments.     

Retroactive interference in rat radial maze performance: The role of point of delay interpolation and the similarity and amount of interpolated material

The conditions necessary for producing retroactive interference (RI) were examined in a 12-arm radial maze. Rats were first given either three or nine forced choices in a to-be-remembered maze. During a 2-h delay, they received one or two trials in a second 12-arm maze, located either in a different room or the same room as the to-be-remembered maze. During the postdelay memory test, RI from the interference trials was produced only when nine choices had been made in the to-be-remembered maze and two interference trials had been conducted during the delay interval. RI was not found when only three forced choices had to be retained or after a single interference trial. The similarity between the interpolated and to-be-remembered mazes had no effect on choice accuracy. It was concluded that two conditions are required for the production of RI in the radial maze. First, a “large amount” of information should be resident in working memory. Second, a substantial number of interpolated trials or choices must be made during the delay.     

The effect of redundant contextual stimuli on autoshaping the pigeon’s keypeck

Three experiments investigated the effect of contextual and trial stimulus lighting conditions on keypeck autoshaping in pigeons. White illumination of a response key before food presentation readily produced keypecking in a brightly lit chamber but failed to do so in a chamber without house illumination (Experiments I and III). Keypecking in a darkened cubicle progressively increased and the facilitatory effect of a houselight decreased as the keylight stimulus was varied from a color change (Experiment II) to a feature change (Experiment III). These findings support a “cue localization” hypothesis of autoshaping. according to which reinforcement signals select specific behaviors for expression and direct these behaviors toward the source of stimulation. This account was extended to superstitious and operant conditioning situations.     

Retrieval of memory in the pigeon by context manipulations

In Experiment 1, 12 pigeons were given eight sessions of VI single stimulus training with a color in a particular context followed by eight sessions of similar training with a line angle in another context. On the next day, half of the subjects were tested for wavelength and angularity generalization in each of the two contexts, a procedure that was thus consistent with training for one dimension and inconsistent for the other. The subjects made significantly more responses to each training stimulus under the consistent context condition, but there was no difference in absolute or relative generalization slopes. In Experiment 2, 12 pigeons were trained as in Experiment 1, but during generalization testing they were exposed to both contexts sequentially. Under the consistent context condition, the subjects responded more to the two training stimuli and yielded sharper absolute and relative wavelength generalization gradients: Under the inconsistent context condition, responding to the training wavelength was substantially disrupted. Thus, under appropriate testing conditions, contextual control over both the amount and the selectivity of responding can be demonstrated.     

Attenuation by contextual cues of retroactive interference of a conditional discrimination in rats

We investigated the effect of associating unique contextual cues with an interpolated learning task on retroactive interference in long-term memory. Rats were originally trained in a two-bar operant chamber with an auditory conditional discrimination stimulus. During interpolated learning, which occurred in either the original or a new context, some rats were trained on a probability learning task that did not include the auditory stimuli present during original learning. Subsequent retraining on the original conditional discrimination task in the original context showed that (1) significant retroactive interference occurs in rats, and (2) the presence of unique contextual cues during interpolated learning significantly reduces this interference. These results extend the conditions under which the susceptibility to retroactive interference can be altered by contextual cues.     

Enhancement and reduction of associative retroactive cue interference by training in multiple contexts

Retroactive cue interference refers to situations in which a target cue X is paired with an outcome in phase 1 and a nontarget cue Z is paired with the same outcome in phase 2, with less subsequent responding to X being seen as a result of the phase 2 training. Two conditioned suppression experiments with rats were conducted to determine whether retroactive cue interference is similarly modulated by a manipulation that influences retroactive outcome interference (e.g., extinction). Both experiments used an ABC renewal-like design in which phase 1 training, phase 2 training, and testing each occurred in different contexts. Experiment 1 found that training the target association in multiple contexts without altering the number of training trials during phase 1 decreased retroactive cue interference (i.e., increased responding consistent with the target association). Experiment 2 found that training the interfering association in multiple contexts without altering the number of interference trials during phase 2 increased retroactive cue interference (i.e., decreased responding consistent with the target association). The possibility of similar mechanisms underlying cue interference and outcome interference is discussed.     

The development of visual short-term memory capacity in infants

Four experiments assessed visual short-term memory capacity in 4- to 13-month-old infants by comparing their looking to changing and nonchanging stimulus streams presented side by side. In each stream, 1 to 6 colored squares repeatedly appeared and disappeared. In changing streams, the color of a different randomly chosen square changed each time the display reappeared; the colors remained the same in nonchanging streams. Infants should look longer at changing streams, but only if they can remember the colors of the squares. The youngest infants preferred changing streams only when the displays contained one object, whereas older infants preferred changing streams when the displays contained up to 4 objects. Thus, visual short-term memory capacity increases significantly across the first year of life.