HSP70和MGMT在乳腺肿瘤中的表达和意义

目的:探讨乳腺肿瘤组织中热休克蛋白HSP70和DNA修复酶O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methyguanine-DNA methytransferase,MGMT)的表达及临床意义。方法:采用免疫组织化学SABC法检测65例乳腺肿瘤组织中HSP70和MGMT的表达情况。结果:乳腺肿瘤中HSP70的阳性率为80.00%(52/65),MGMT的阳性率为60.00%(39/65),二者呈中度相关性(r=0.46)。其中,有淋巴结转移组的阳性率高于无转移组(P<0.05)。结论:HSP70和MGMT的表达率与淋巴结转移情况有关。检测HSP70与MGMT对乳腺肿瘤预后和化疗方案的选择有指导意义。     

Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid

Objective： To investigate whether vascular endothelial growth factor （VEGF） gene plasmid carried by polytetrafluoroethylene （PTFE） vascular graft materials could transfect endothelial cells （ECs） and promote their growth. Methods： PTFE vascular graft materials carried with pCDI-hVEGF121, pCDI or pEGFP were incubated in Tris-buffer solution and the values of optical density of 260 nm at different time were plotted, then the DNA controlled release curve was made. ECs derived from human umbilical vein were seeded on the pCDI-hVEGF121/pCDI/pEGFP-PTFE materials or tissue culture plates, ECs numbers were counted and VEGF protein concentrations at different time were measured by enzyme-linked immunoadsorbent assay method. Green fluorescent protein （GFP） expression in ECs on pEGFP-PTFE materials was examined with fluorescence mi- croscopy. Results： The controlled release curve showed that the gene released from PTFE materials was rapid within 8 h, then slowed down and that the gene released continuously even after 72 h. At 24, 72 and 120 h, ECs number and proliferation rate of pCDI-hVEGFI21-PTFE materials were higher than those ofpCDI or pEGFP-PTFE materials （P〈0.05）. VEGF protein concentration of pCDI-hVEGF121-PTFE materials was higher than that of pC DI or pEGFP-PTFE materials at 6, 24, 72 and 120 h （P〈0.01）. GFP expression in ECs on the pEGFP-PTFE materials could be detected by fluorescence microscopy. Conclusion： PTFE graft can be used as a carrier of VEGF gene plasmid, VEGF gene carried by PTFE can transfect ECs and promote ECs growth.     

结核分枝杆菌热休克蛋白65的最新研究进展

结核病(tuberculosis,TB)是一种由结核分枝杆菌引起的人兽共患的慢性传染病。而HSP(heat shock protein)是一类在原核和真核细胞中高度保守的蛋白质,在正常细胞的蛋白转位、折叠和装配过程中起着分子伴侣的作用。目前在MTB中研究最多的是HSP65,他的细胞表位较多,是机体免疫系统识别的重要细胞内抗原。     

ELISA法检测胰岛素依赖型糖尿病患者血清中64KD抗体最佳条件的选定

本文报告了用自己纯化的BCG65KD热休克蛋白(HSP65)作抗原建立ELISA法检测胰岛素依赖型糖尿病(IDDM)患者血清中抗胰岛细胞64KD蛋白抗体(简称64KD抗体)并对其最适检测条件进行了选定,结果表明,最适条件:BCGHSP65抗原包被浓度为10μg/mL,1:100血清37℃温育2h,辣根过氧化物酶标记的羊抗人IgG(SAH-IgG-HRP)稀释度为1:800,2％BSA-PBS-T作稀释液。     

HSP27和HSP70mRNA在大肠癌中表达的临床意义

目的:探讨HSP27和HSP70 mRNA在大肠癌中表达的临床意义。方法:取大肠癌标本77例,采用免疫组织化学方法检测HSP27的表达状况,从中选取36例手术标本,采用核酸原位杂交技术(ISH)检测HSP70 mRNA的表达。结果:HSP27和HSP70 mRNA在大肠癌中的阳性率分别为40.3%和66.9%,HSP27和HSP70 mRNA均与大肠癌的分化程度相关,与发生部位、浸润深度及淋巴结转移无明显相关性。结论:检测HSP27和HSP70 mRNA可作为大肠癌患者预后的一个重要指标。     

热休克蛋白(HSP70、HSP27)在食管癌组织中表达的意义

目的 :探讨HSP70和HSP2 7在食管癌组织中的表达情况。方法 :采用SP免疫组织化学染色检测 5 9例食管癌组织中HSP70和HSP2 7。结果 :HSP70和HSP2 7的总阳性率分别为 6 4.4 1% (38/5 9)和 5 8.6 2 % (34/5 8) ,HSP70和HSP2 7的共同阳性率为 30 .0 0 % (9/30 )。结论 :在食管癌中HSP70和HSP2 7普遍表达。     

HSP27和HSP70在膀胱癌中的表达和意义

目的:研究HSP27和HSP70在膀胱癌中的表达和意义.方法:应用免疫组化SP法,检测73例膀胱癌组织中的HSP27和HSP70的表达.结果:HSP27和HSP70在膀胱癌中的阳性表达率分别为45.21%(33/73)和34.25%(25/73).HSP27的表达随分级和分期呈降低趋势,但组间无明显性差异(P＞0.05);HSP70的表达与膀胱癌的分级和分期呈正相关(P＜0.05).结论:HSP27和HSP70的表达可作为判断膀胱癌恶性程度的指标,并为肿瘤化学治疗提供依据.     

热休克蛋白70生物学功能研究的新进展

<正> 热休克蛋白(heat shock protein,HSP)是所有原核细胞和真核细胞在高温或应激情况下所产生的一组具有高度保守性的蛋白质,又称应激蛋白(stress protein).应激刺激包括环境方面的刺激(紫外线、热休克、重金属和氨基酸等)、病理方面的刺激(病毒感染、细菌感染、寄生虫感染、发热、炎症、肿瘤或自身免疫等)和生理方面的刺激(生长因子、细胞分化、激素刺激或组织发育等)均可诱导细胞内HSP合成增加.根据HSP分子量不同,HSP分为HSP90、HSP70、HSP60和小分子量HSP等四个家族,此外还有分子量100～110KD而性质不同与上述家族的大分子HSP.HSP70在正常细胞中水平较低,在应激状态下显著升高[1].HSP70不仅参与许多正常生理活动(如分子伴侣作用),而且与一些疾病密切相关(如肿瘤).     

山东省荣成市第二十七中学

山东省荣成市第二十七中学建于1993年8月,位于俚岛镇驻地,占地65亩,建筑面积8868平方米,绿地面积21810平方米,绿化覆盖率达50%以上,现有32个教学班,学生1300多人,教职工121人,其中专任教师118人,学历达标率为98%。     

预热处理对大鼠急性运动股四头肌保护作用的实验研究

目的:通过观察预热处理前后大鼠急性运动股四头肌热休克蛋白70(HSP70)、超氧化物歧化酶(SOD)、丙二醛(MDA)的变化,探讨预热处理诱导HSP70表达对运动机体的保护作用.方法:三月龄雄性SD大鼠随机分为室温安静组、室温运动后即刻组、室温运动后3 h组、预热处理安静组、预热处理运动后即刻组、预热处理运动后3 h组.预热处理组进行热暴露处理后,室温恢复24 h后,运动组大鼠进行一次急性运动,运动后即刻和3h进行取样,对股四头肌HSP70的含量、SOD活性、MDA含量进行测试.结果:(1)预热处理安静组HSP70含量明显高于室温安静组(P<0.05)(2)室温组运动后即刻股四头肌HSP70有上升的趋势,但与安静组比较没有显著性的差异,预热处理组运动后即刻HSP70的含量与预热处理安静组比较有显著性的差异,运动后3 h,两组HSP70的表达都明显增加.(3)运动后即刻股四头肌SOD的活性明显升高,3 h后其活性降低,但与安静组比较仍具有显著性差异.在变化过程中预热处理组SOD活性高于室温组.(4)运动后即刻股四头肌MDA的含量明显升高,3 h后减少,在运动后前3 h,预热处理组MDA的清除比室温组迅速,有显著性差异.以上结果表明预热处理诱导HSP70的大量表达可提高运动时SOD活性,降低MDA的含量.提示HSP70可能会提高机体的抗氧化能力,减少自由基对机体的损害,对运动机体具有一定的保护作用.     

胆管癌中p27Kip1、VEGF的表达、意义及相互关系

目的 :探讨 p2 7Kip1、VEGF在胆管癌中的表达及相互关系。 方法 :采用免疫组化技术检测 32例胆管癌标本中p2 7Kip1及VEGF的表达。结果 :p2 7Kip1、VEGF在胆管癌和正常胆管组织中的阳性表达率分别为 5 6 .2 5 %和 11.1% (P <0 .0 1) ;81.2 5 %和 33.3% (P <0 .0 1)。p2 7Kip1、VEGF在胆管癌中的表达具有相关性 (P <0 .0 5 ) ,与分化程度、转移水平有关 ,与大小、生存期无关。结论 :p2 7Kip1、VEGF在胆管癌中高表达 ,p2 7Kip1、VEGF与胆管癌的发生、发展有关。     

(C_6H_6ClNO_3S)_2(H_2O)_2的合成和晶体结构

合成了一种新的化合物(C6H6C lNO3S)2(H2O)2,测定了其结构.晶体数据表明该配合物属三斜晶系,P-1空间群,晶胞参数为:a=5.8626(8),b=11.7993(17),c=12.7680(18),α=88.286(2)°,β=87.534(2)°,γ=84.121(2)°,V=877.5(2)3,Z=2,Dc=1.708 mg/m3,Rgt(F)=0.0418,wR2(F2)=0.0974,F(000)=464,数据完整度为99.3%,标题化合物含有3种氢键,形成一种三维网状的稳定结构.     

麻风树核糖体失活蛋白的基因组DNA分离及其转化烟草分析

1IntroductionRibosome-inactivating proteins(RIPs)occur natu-rally in a variety of higher plant species,and theyfunction by catalytic depurination of a specific aden-osine residue located near the3*terminus of eukary-otic large ribosomal subunit rRNA,preventing EF-2/GTP binding and thereby blocking peptidyl-tRNAtranslocation during protein synthesis[1].Many RIPsare potent antiviral and antifungal proteins in vitro[2,3],but it may beinsufficient for field application,as com-pared to the ac…     

Diversity arrays technology (DArT) for studying the genetic polymorphism of flue-cured tobacco (Nicotiana tabacum)

Diversity arrays technology (DArT) is a microarray-based marker system that achieves high throughput by reducing the complexity of the genome. A DArT chip has recently been developed for tobacco. In this study, we genotyped 267 flue-cured cultivars/landraces, including 121 Chinese accessions over five decades from widespread geographic regions in China, 103 from the Americas, and 43 other foreign cultivars, using the newly developed chip. Three hundred and thirty polymorphic DArT makers were selected and used for a phylogenetic analysis, which suggested that the 267 accessions could be classified into two subgroups, which could each be further divided into 2–4 sections. Eight elite cultivars, which account for 83% of the area of Chinese tobacco production, were all found in one subgroup. Two high-quality cultivars, HHDJY and Cuibi1, were grouped together in one section, while six other high-yield cultivars were grouped into another section. The 330 DArT marker clones were sequenced and close to 95% of them are within non-repetitive regions. Finally, the implications of this study for Chinese flue-cured tobacco breeding and production programs were discussed.     

血管内皮生长因子在乳腺良、恶性肿瘤表达的临床意义

目的：探讨血管内皮生长因子（VEGF）在乳癌中过表达的临床意义。方法：取乳腺腺瘤47例,乳腺癌95例,利用SABC法检测VEGF阳性率。结果：VEGF在腺瘤中阳性率为25.53%（12/47）,在乳癌中阳性率为54.75%（52/95）;在浸润性癌和有淋巴结转移组阳性率明显高于非浸润性组和无淋巴结转移组（P〈0.05）。结论：VEGF过表达提示患者预后不良,检测VEGF对化疗方案选择有指导意义。     

Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma

Objective: To investigate the relationship between the expression of endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) and angiogenesis in primary astrocytoma. Methods: Thirty-seven primary astrocytomas and 4 astrocytic hyperplasia samples were collected and divided into three groups according to histological grade. The expression of eNOS, VEGF and factorⅧrelated antigen (FⅧRAg) were assayed by immunohistochemistry. Microvascular density was assessed by FⅧRAg immunoreactivity. The intensity of immunoreactivity was graded according to the percentage of positive tumor cells. Results: No eNOS and VEGF were expressed in the astrocytes and vascular endothelium in astrocytic hyperplasia. The expression of eNOS or VEGF was light in low-grade astrocytoma and strong in glioblastoma. eNOS expression in astrocytoma was very positively correlated with VEGF. eNOS and VEGF expression in anaplastic astrocytoma was median in contrast to the low grade astrocytoma and glioblastoma. Lower microvascular density was found in low grade astrocytoma than that in higher grade malignant ones. The expressions of eNOS and VEGF were correlated with microvascular density and tumor malignancy. Conclusion: This finding suggests that eNOS and VEGF may have cooperative effect in tumor angiogenesis and play an important role in the pathogenesis of primary astrocytoma.     

VEGF在膀胱移行细胞癌中的表达及临床意义

目的：探讨血管内皮生长因子（VEGF）在膀胱移行细胞癌中的表达及临床意义。方法：采用免疫组化、ELISA方法对45例膀胱移行细胞癌组织、血浆和10例正常膀胱黏膜组织、30例正常成人血浆中VEGF进行检测分析。结果：VEGF在膀胱移行细胞癌患者中呈高表达，且随分期、分级的增高而增高；膀胱癌手术前患者血浆中VEGF的浓度高于手术后。结论：1．VEGF的表达与膀胱移行细胞癌的病理分级、临床分期及生物学行为有密切关系；2．血浆中VEGF水平可有助于预测膀胱癌患者手术疗效。     

Inducible nitric oxide synthase expression is related to angiogenesis,bcl-2 and cell proliferation in hepatocellular carcinoma

In this study, we examined the expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) by immunohistochemical staining in 76 tissue sections collected from hepatocellular carcinoma (HCC) patients undergoing hepatectomy. Microvascular density (MVD) was determined by counting endothelial cells immunostained using anti-CD34 antibody. We performed DNA-flow cytometric analyses to elucidate the impact of iNOS and VEGF expression on the cell cycle of HCC. Most of the HCC cells that invaded stroma were markedly immunostained by iNOS antibody. The iNOS stain intensity of the liver tissue close to the tumor edge was stronger than that of HCC tissue, and the strongest was the hepatocytes closer to the tumor tissue. However, iNOS expression in 10 normal hepatic samples was undetectable. VEGF positive expression ratio was 84.8% in iNOS positive expression cases, and the ratio was 35.3% in negative cases. There was significant correlation (P=0.000) between iNOS and VEGF expression. Moreover, iNOS expression was significantly associated with bcl-2 and MVD, but without p53 expression. DNA-flow cytometric analyses showed that combined expression of iNOS and VEGF had significant impact on the cell cycle in HCC. PI (Proliferating Index) and SPF (S-phase fraction) in the combined positive expression of iNOS and VEGF group was significantly higher than that in the combined negative group. The present findings suggested that iNOS expression was significantly associated with angiogenesis, bcl-2 and cell proliferation of HCC. Project supported in part by the National Ninth-Five-Years Project Fund (No. 96909121), China     

Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma

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