Purification and relationship with gastric disease of a 130 kDa (CagA) protein of<Emphasis Type="Italic">Helicobacter pylori</Emphasis>

Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein ofH. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic ulcer disease, 2 with gastric cancer) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44), atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7), gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ²=13.327,P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (r _s=0.442,P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases. Project supported by the China Medical Board (96-628) and Zhejiang Province Hygiene Bureau (2000 A055)     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1.     

718例纤维胃镜活检组织检查结果

本文通过718例纤维胃镜活检组织险查结果表明,胃病的发病情况慢性浅表性胃炎>慢性萎缩性胃炎>胃溃疡>胃息肉>糜烂性胃炎>胃癌,其中以慢性浅表性胃炎患病率最高占55%。胃镜与病理诊断符合率81.06%,男女比为3:1。浅表性胃炎的程度越重合并病变越多,对中度以上不典型增生的高危人群应定期检查,以便及时治疗。     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients withHelicobacter pylori infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) andHelicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status ofH. pylori was determined by histologic examination, RUT,¹⁴C-UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients withH. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml,P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection ofH. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml, respectively (P<0.05). The serum levels of sICAM-1, proved to be significantly correlated with the density ofH. pylori colonization in gastric mucosa (r _s=0.316,P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated thatH. pylori infection up-regulates the expression of sICAM-1.     

消化性溃疡并出血108例临床与内镜分期对比观察

目的:通过内镜观察消化性溃疡并出血在临床发作期与缓解期有无对应关系来确定治疗.方法:采用欧林巴斯电子胃镜GIF-V观察消化性溃疡活动期、愈合期和疤痕期出血情况.结果:消化性溃疡活动期出血占63.00%(68/108),愈合期30.00%(32/108),疤痕期占7.00%(8/108).结论:消化性溃疡临床发作期应包括内镜下的活动期和部分愈合期及少数疤痕期.临床分期与内镜分期无明显的对应关系,内镜示为疤痕期而临床症状明显的,也应按发作期进行治疗并延长治疗的疗程.     

Distribution of H.pylori antigens in gastric mucosa and its significance

Objective: To investigate the distribution of H. pylori antigens in the gastric mucosa in patients with H. pylori infection, and the relationship between the distribution and gastric cancer. Methods: Of 112 patients confirmed by pathological study to have chronic superficial gastritis, precancerous changes (chronic atrophic gastritis, intestinal metaplasia or atypical hyperplasia) and gastric cancer, 28 were H. pylori negative and 84 were H. pylori positive. H. pylori antigens in the gastric mucosa were detected by immunohistochemistry. Results: The H. pylori positive group, comprised 12 of 22 (50.0%) in the chronic superficial gastritis group, 22 of 25 (88.0%) in the precancerous changes group and 13 of 35 (37.1%) in the gastric cancer group. The positive rates of H. pylori antigens in the cytoplasm progressively increased, respectively at 0.0% (0/12),63.6% (14/22) and 84.6% (11/13) for the same groups (χ2=19.76, P=0.000); H.pylori antigens were located in the mucus layer and above the neck of the mucosal gland in 9 of 12 (75.0%) cases with chronic superficial gastritis, at the neck of the mucosal gland and the isthmus in 12 of 22 (54.5%) cases with precancerous changes, below the isthmus in 9 of 13 (69.2%) cases with gastric cancer (x2=25.30, P=0.000). In the H. pylori negative group, no H.pylori antigen was observed. Conclusion: With the progression of chronic superficial gastritis→precancerous changes→gastric cancer, H. pylori antigens progressively migrated from the outer part to the inner part of the cell, and from the superficial to the deep gastric mucosa.     

Distribution of H.pylori antigens in gastric mucosa and its significance

INTRODUCTION Helicobacter pylori (H.pylori) was grouped as a class I carcinogen by the International Agency for Research on Cancer in 1994. A direct relation be-tween H.pylori infection and gastric carcinogenesis was demonstrated in 1998 in an experimental animal model (Watanabe et al., 1998). However, the role of H.pylori in human gastric carcinogenesis is sup-ported almost exclusively by epidemiological data and prospective histopathological studies. So far the mechanism of H.pylor…     

Morphological and pathologic changes of experimental chronic atrophic gastritis （CAG） and the regulating mechanism of protein expression in rats

INTRODUCTION In 1998 gastrointestinal pathologists reached aconsensus on the definition of chronic atrophic gas- tritis (CAG), which was described as programmed loss of gastric gland and/or replacement by intestinal glands in gastric mucosa. CAG was recognized to be closely related with development of gastric cancer and listed as precancerous lesions in this meeting (Genta, 1998). According to Correa (1992)’s cascade of gas-tric carcinogenesis, gastric cancer was believed to develop fr…     

Expression of three essential antioxidants of Helicobacter pylori in clinical isolates

Objective

Helicobacter pylori maintains long-term persistence in the host and combats oxidative stress via many antioxidant proteins, which are expected to be relevant to bacterial-associated gastric diseases. We aimed to investigate the expression of three essential antioxidants in H. pylori strains isolated from patients with different clinical outcomes. Methods: Forty H. pylori strains were isolated from endoscopic biopsy specimens of gastric mucosa from 13 patients with gastric cancer, 13 with peptic ulcer, and 14 with gastritis. The expression of thioredoxin 1 (Trx1), arginase (RocF), and alkyl hydroperoxide reductase (AhpC) in H. pylori was measured by real-time PCR. Comparisons among multiple sample sets were analyzed using a one-way ANOVA test. Pearson’s correlation test was used to assess relationships among multiple continuous variables.

Results

Trx1 expression of H. pylori in gastric cancer and peptic ulcer tissues was higher than that in tissues with gastritis. RocF expression of H. pylori in gastric cancer tissues was higher than that in tissues exhibiting peptic ulcer and gastritis. However, we did not find any differences in AhpC expression in samples from patients with different clinical outcomes. The expression of Trx1 and RocF had a positive, linear correlation. The expression of Trx1 and AhpC had a positive correlation without a linear trend. We found no correlation between the expression of RocF and AhpC.

Conclusions

Our observations indicate that the expression of Trx1 and RocF in H. pylori might be related to gastric carcinogenesis. In H. pylori, the expression of members of the antioxidant system may be correlated and relevant to gastric cancer.     

消化性溃疡与蛋白质

认为消化性溃疡（Pepticulcer）病的发生、治疗和预防与食物中蛋白质的缺乏有着极为密切的关系,技正了“已经误导我们将近一个世纪的‘无酸就无溃疡’（Noacid,Noulcer）的论点”,并且指出中和胃酸和抑制胃酸的药物不可能根治消化性溃疡,只有向食物中添加足够量的蛋白质或蛋白质降解产物才是治疗和预防消化性溃疡的根本之所在！     

幽门螺旋杆菌感染与耳鼻咽喉头颈外科疾病关系研究综述

幽门螺旋杆菌与慢性胃炎及消化性溃疡密切相关,但最近研究发现与某些耳鼻咽喉头颈外科疾病亦有一定关系。本文从阐述幽门螺旋杆菌的生物学性状、诊断技术、流行病学及抗幽门螺旋杆菌治疗入手,综述了幽门螺旋杆菌与耳鼻咽喉头颈外科疾病的关系,为从系统因素出发研究耳鼻咽喉头颈外科疾病提供新思路。     

Distribution of<Emphasis Type="Italic">H. pylori</Emphasis> antigens in gastric mucosa and its significance

Objective: To investigate the distribution ofH. pylori antigens in the gastric mucosa in patients withH. pylori infection, and the relationship between the distribution and gastric cancer. Methods: Of 112 patients confirmed by pathological study to have chronic superficial gastritis, precancerous changes (chronic atrophic gastritis, intestinal metaplasia or atypical hyperplasia) and gastric cancer, 28 wereH. pylori negative and 84 wereH. pylori positive.H. pylori antigens in the gastric mucosa were detected by immunohistochemistry. Results: TheH. pylori positive group, comprised 12 of 22 (50.0%) in the chronic superficial gastritis group, 22 of 25 (88.0%) in the precancerous changes group and 13 of 35 (37.1%) in the gastric cancer group. The positive rates ofH. pylori antigens in the cytoplasm progressively increased, respectively at 0.0% (0/12), 63.6% (14/22) and 84.6% (11/13) for the same groups (χ ²=19.76,P=0.000);H. pylori antigens were located in the mucus layer and above the neck of the mucosal gland in 9 of 12 (75.0%) cases with chronic superficial gastritis, at the neck of the mucosal gland and the isthmus in 12 of 22 (54.5%) cases with precancerous changes, below the isthmus in 9 of 13 (69.2%) cases with gastric cancer (χ ²=25.30,P=0.000). In theH. pylori negative group, noH. pylori antigen was observed. Conclusion: With the progression of chronic superficial gastritis→precancerous changes→gastric cancer,H. pylori antigens progressively migrated from the outer part to the inner part of the cell, and from the superficial to the deep gastric mucosa.     

The relationship between ulcer recurrence andHelicobacter pylori: A prospective one-year follow-up study in China

To study the relationship between ulcer recurrence andHelicobacter Pylori. 147 patients with peptic ulcer were divided intoH. Pylori infected group andH. pylori uninfected group by rapid urease test serum antibody examination and histopathology of gastric biopsy specimens. We assigned all patients to receive dual oral therapy with omeprazole 40 mg/day for 4 weeks and amoxicillin 2000 mg/day for 2 weeks. To all patients, repeated gastroscope was performed at 8 weeks and one-year after beginning therapy to record whether the ulcer was cured. Endoscopic biopsy was also performed to determineH. pylori status. One year later,H. pylori positive patients had a higher ulcer recurrent rate thanH. pylori negative patients (23. 33% vs 2. 70%; P=0.001). Ulcer recurrence was closely related toH. pylori infection.H. pylori was the risk factor for ulcer relapse.     

溃疡病胃窦G和D细胞的体视学分析

应用真彩色医学图象分析仪,对15例DU、GU和Con组患者的胃窦粘膜肉眼下非病变区30张切片,随机选择,经免疫组化双重染色的G和D细胞300个,进行形态定量分析。每个细胞检测12个参数,各项参数都行统计学t检验两两相比处理。在三组中,G细胞的周长、面积、长径、短径、比表面、平均截距、平均体积、平均表面积、圆球度、平均直径和D细胞的长径、短径、形状因子、平均轴比的显著差异率均在60％以上,这些结果,建立了溃疡病非病变区胃窦粘膜幽门腺G和D细胞的体视学形态参数数据库,并为它们参与内分泌调节提供了客观依据。     

P_(53)、PCNA在胃粘膜中的表达及其与胃癌发生的关系

目的为探讨P_(53)、PCNA抗原在胃粘膜中的表达情况及其与胃癌发生的关系方法：应用LsAB免疫织化方法对80例胃粘膜进行了检测结果显了胃癌中P_(53)、PCNA强阳性表达率（80％、93．3％）明显高于异型增生（467％,53．3％）异型增生中P_(53)、PCNA强阳性表达率明显高于慢性萎缩性胃炎及慢性浅表性胃炎．结论研究结果表明,检测P_(53)、PCNA在肖粘膜中的不同表达,对胃癌发生机理的认识有重要意义．     

31例糖尿病合并消化性溃疡的临床分析

目的:分析比较糖尿病合并消化性溃疡的临床特点。方法:采用纤维胃镜对127例糖尿病(检出消化性溃疡31例)与同期非糖尿病153例(检出消化性溃疡43例为对照组)进行观察。结果:糖尿病合并消化性溃疡总患病率与对照组比较无显著性差异(P>0.05);糖尿病合并消化性溃疡组食后腹胀症状多于对照组(P<0.05)。而上腹痛、恶心呕吐,返酸嗳气等症状明显低于对照组(P<0.01);糖尿病合并胃溃疡,复合溃疡发生率高于对照组(P<0.05),合并十二指肠溃疡明显低于对照组(P<0.01),糖尿病合并消化性溃疡发病与糖尿病病程无关。     

乌鳢鱼卵中胰蛋白酶抑制剂的分离纯化及性质研究

以乌鳢鱼卵为材料,通过生物化学手段和研究方法,从乌鳢鱼卵匀浆液中分离得到了一种相对分子质量大约为42kDa的蛋白酶抑制剂．该蛋白对胰蛋白酶具有专一性的抑制活性,最低抑制浓度为130μg／mL,对胰蛋白酶的抑制常数为30．3nM．SDS—PAGE电泳检测表明,该抑制剂为不合二硫键的单链蛋白．热稳定性和酸碱稳定性研究表明,该蛋白酶抑制剂在30℃～100℃温度下能保持76％以上的活性,在pH值2～11的酸碱条件下,能保持90％以上的活性。     

低频脉冲电流治疗慢性胃炎和溃疡病48例

本文报告了用低频脉冲电流治疗慢性胃炎和溃疡病48例。其中治愈38例,显著好转6例,好转2例,无效2例。文中列举了2个典型病例,并讨论了治疗机理。     

大鼠慢性萎缩性胃炎模型的建立

目的 :建立稳定、价廉、实用的慢性萎缩性胃炎动物模型。方法 :采用 2 %水杨酸钠和30 %酒精混合溶液灌胃 ,并结合劳累、饥饱失常等多因素方法刺激Wistar大鼠胃粘膜 8周。结果 :CAG模型组大鼠较正常对照组大鼠体重明显减轻、胃粘膜腺体萎缩、炎细胞浸润 ,壁细胞数量减少且部分呈空泡样变性 ,停止作用 4周后仍无恢复。结论 :多因素联合作用 8周能建立良好的大鼠慢性萎缩性胃炎模型 ,且稳定性好。     

Recurrent severe vomiting due to hyperthyroidism

Thyrotoxicosis may present in many ways; severe vomiting as a prominent symptom of thyrotoxicosis is uncommon. In this paper, we report a 24-yearoold Chinese male with hyperthyroidism who presented with recurrent severe vomiting. The patient had had intermittent vomiting for seven years and had lost approximately 15 kg of weight. Gastroscopic examinations revealed chronic gastritis and one occasion peptic ulcer. He was treated with antacid and proton pump inhibitors, but his symptoms had no relief. His presenting symptoms suggested hyperthyroidism and were confirmed by laboratory data. After a month of propylthiouracil therapy, his symptoms were relieved. It should be noted that hyperthyroidism patients can have unexplained vomiting, and that hyperthyroidism may coexist with peptic ulcer in rare cases. Awareness of such atypical presentations of hyperthyroidism may help to make a correct diagnosis.