优化体育教学 培养学生抗挫能力

<正>体育教学是以锻炼身体为基本形式,对学生进行基本知识、基本技能和基本技术的教育,同时还对学生进行良好的心理品质和社会适应能力的培养。在体育教学中,学生的意志品质、心理活动以及个性特点,都表现的极其直观、具体、生动、鲜明,因此在体育教学中对学生进行抗挫折能力的培养具有十分重要的意义。然而,怎样优化体育教学,培养学生的抗挫能力呢?一、转变观念是实现目标优化、培养抗挫能力的前提     

文化产业在经济危机中的抗衰性研究

经济危机发生后,以满足人们物质需求为目的的传统产业会受到重大冲击.与此不同,文化产业却在经济危机中逆势增长,表现出明显的抗衰性.经济危机成了文化产业发展的契机.文化产业的抗衰性.既为减缓经济衰退做出了直接贡献,又具有精神抚慰和激励作用,为人们战胜经济衰退提供了精神力量.文化产业之所以在经济危机中具有抗衰性,是因为文化产品和文化服务具有"口红效应",文化产业具有相对稳定性和优化产业结构、转变经济增长方式的功能.因此,我国要充分发挥文化产业的抗衰性作用.努力建设成文化产业强国.     

基于离散余弦变换的视频数字水印算法研究

针对嵌入水印后视频容易遭受压缩攻击的难题,提出了一种基于离散余弦变换的抗攻击水印方法。介绍了变换域水印算法及其基于离散余弦变换的数字水印算法,对现有的余弦变化水印算法优缺点进行了讨论,提出了基于块内系数自适应优化的水印嵌入算法,并通过实验仿真验证。结果证明,算法对于压缩攻击具有较强的鲁棒性。     

高层建筑剪力墙结构应用的铝合金模板施工技术优化

为优化铝合金模板施工技术,提升建筑施工过程的建筑承载性能和抗震性能,研究高层建筑剪力墙结构应用的铝合金模板施工技术优化方法。使用铝合金模板一次性成型结构并校正模板垂直度,优化铝合金模板施工技术,设计对比实验测试优化前后剪力墙试件的荷载性能,并分析试件优化前后层间侧移刚度与抗震性能。实验结果证明：与未优化试件相比,优化后试件的相对和绝对位移较小,具有更高的承载性能与持荷性能,能够满足施工要求与安全要求,且优化后试件的抗侧刚度和抗震性能有较大提升。     

水杨酸诱导植物抗逆性研究进展

水杨酸作为一种新型的植物激素,在植物抗性方面具有重要的作用。本文介绍了水杨酸在植物抗病性、抗旱性、抗盐性、抗高温、抗寒性、抗重金属胁迫、抗臭氧胁迫和抗紫外辐射方面作用的研究。     

基于ABAQUS的某款SUV后背门抗凹性能分析

运用Hypermesh和ABAQUS等软件,对后背门内外板凹陷过程进行了模拟。采用数值模拟的方法分析其抗凹性,并使用此方法来验证优化方案的可行性。研究结果表明,有限元方法可以较好地预测和评估后背门的抗凹性能,为后背门的优化设计和材料选取提供理论指导和数据依据。     

小学生抗挫能力培养途径探微

培养学生的抗挫能力需要教师多重考量和设计。在具体教学中,教师要强化抗挫能力的培养意识,精选引导方法,优化教育环境,拓宽训练域度,从不同维度、不同视角展开教学实践活动,以有效提升学生的抗挫能力,促进学生身心健康发展。     

抗蛇行减振器布置方式对机车横向动力学性能影响：Pareto优化和参数分析（英文）

目的：以中国某型Bo-Bo高速机车为研究对象,分析四种典型的抗蛇行减振器布置方式及横向安装角对机车横向动力学性能和参数匹配关系的影响,并解释其作用机理。创新点：1.通过多目标优化方法来同时优化机车低锥度和高锥度横向稳定性,获得四种抗蛇行减振器布置方式下机车最优横向动力学性能及横向安装角的不同选取原则;2.当抗蛇行减振器横向安装角存在时,引入抗蛇行减振器附加作用力和作用力矩,结合车体横向和摇头模态相位差来解释抗蛇行减振器布置方式对机车蛇行稳定性和车体前后横向平稳性差异的影响机理。方法：1.基于搭建的MATLAB/SIMPACK联合仿真平台,采用多目标优化方法得到机车最优横向动力学性能及对应悬挂参数分布结果（图4和5）;2.通过拉丁超立方采样对机车直线运行性能和曲线通过性能评价指标进行蒙特卡洛仿真,并采用基于径向基函数的高维模型表示的敏感性分析方法对关键悬挂参数进行全局敏感性分析（图6）;3.采用根轨迹法分析不同抗蛇行减振器布置方式下横向安装角对机车蛇行稳定性影响规律,并提取蛇行模态中对应的车体横移和摇头模态相位差（图8和9）。结论：1.抗蛇行减振器的布置方式对机车横向稳定性和平稳性具有显...     

基于智能驱动模式的优化控制与检测方法

针对一类具有强烈动态特性的复杂对象,融合智能控制和最优控制方法,并采用软测量技术,提出一种基于智能驱动模式的优化控制与检测方法.基于智能驱动模式的优化控制系统不仅具有一定的优化能力,而且提高了控制系统的鲁棒性.     

基于DSP的四路H.264视频编码器的实现与优化

设计一种基于DM642实现的四路H.264实时视频编码器。给出了系统的软硬件设计和优化方法,提出了编码器关键模块的优化策略。经优化后的编码器能实现四路H.264视频的实时编码,具有功能强、可靠性高、体积小、功耗低和易于升级等特点。     

5-Fluorocytosine–Sugar Conjugates for Glucose Transporter-Mediated Tumor Targeting: Synthesis,Cytotoxicity, and Cellular Uptake Mechanism

Two novel sugar-conjugated 5-fluorocytosine (5-FC) antineoplastic compounds were designed and synthesized to improve the selective drug uptake by targeting the tumor-specific glucose transporter (GLUT).The antitumor activity of these compounds was evaluated in four different human cancer cell lines:A549 (human lung cancer cell line),HT29 (human colorectal cancer cell line),H460 (human lung cancer cell line),and PC3 (human prostate cancer cell line).The sugar conjugates exhibited cytotoxicity similar to or higher than 5-FC and 1-hexylcarbamoyl-5-FC in A549,HT29,H460,and PC3.Furthermore,GLUT-mediated transport of the glycoconjugate was investigated with GLUT inhibitor-mediated cytotoxicity analysis in a GLUT-overexpressing HT29 cell line.The cell-killing potency of 5-FC glycoconjugate was found to depend significantly on the GLUT inhibitor,and the cellular uptake of molecules was regulated by GLUT-mediated transport.All the results demonstrate the potential advantages of glycoconjugation for Warburg effect-targeted drug design.     

UFCI基因在乳腺细胞系中的表达分析

泛素结合酶UFC1是一个新鉴定的类似泛素结合酶E2的基因。目前,对UFC1的功能研究报道还比较少,有待更进一步的研究和探讨。采用半定量RT-PCR技术分别检测一系列的乳腺细胞系MCF7、HBL100、MDA-MB231和MDA-MB-453中UFC1基因的转录水平,结果显示,在正常的HBL100乳腺细胞系中的表达最高,而在乳腺癌细胞系MCF7,MDA-MB231,MDA-MB-453中的表达明显降低。UFC1在乳腺细胞系中的差异表达的结果为将UFC1作为新的靶分子引入乳腺癌的临床预防和治疗提供实验依据。     

血清CA-19-9测定对胰腺癌的诊断价值

目的：寻求胰腺癌的诊断方法，方法：用免疫放射分析法（IRMA）检测血清CA－19－9含量，106例血清CA－19－9含量3700u/L，进行追踪随访，结果：106例中，胰腺癌66例，结肠癌15例，胆管癌13例，肺癌9例，结论：检测血清CA－19－9含量，有利于胰腺癌的诊断和与结肠癌，胆管癌，肺癌等恶性肿瘤的鉴别。     

瑞香狼毒提取物作用于人肺癌细胞NCI-H446[H446]后端粒酶的定量表达

目的:通过端粒酶的数量变化来探讨瑞香狼毒提取物对肺癌细胞NCI-H446[H446]的抗肿瘤作用。方法:体外培养肺癌细胞NCI-H446[H446]细胞株,以不同浓度的瑞香狼毒提取物作用24h后,用荧光标记的方法定量检测端粒酶。结果:瑞香狼毒提取物作用人肺癌细胞提取物NCI-H446[H446]后,端粒酶的数量明显减少并呈现与剂量的正相关性。结论:瑞香狼毒提取物对肺癌细胞NCI-H446[H446]具有明显的抗肿瘤作用。     

Chemokines and their receptors in lung cancer progression and metastasis

Lung cancer is the leading cause of cancer-related mortality around the world. Despite advancements in diagnosis, surgical techniques, and neoadjuvant chemoradiotherapy over the last decade, the mortality rate is still high and the 5-year survival is a dismal 15%. Fortunately, early detection by low-dose computed tomography (LDCT) scans has reduced mortality by 20%; yet, overall, 5-year-survival remains low at less than 20%. Therefore, in order to ameliorate this situation, a thorough understanding of the underlying molecular mechanisms is urgently needed. Chemokines and their receptors, crucial microenvironmental factors, play important roles in lung tumor genesis, progression, and metastasis, and exploring the mechanisms of this might bring new insights into early diagnosis and precisely targeted treatment. Consequently, this review will mainly focus on recent advancements on the axes of chemokines and their receptors of lung cancer.     

Role of LINC00152 in non-small cell lung cancer

Journal of Zhejiang University-SCIENCE B - Non-small cell lung cancer (NSCLC) accounts for about 85% of all lung cancer cases. The pathogenesis of NSCLC involves complex gene networks that include...     

人肺癌细胞阿霉素耐药株的生物学特性

人肺腺癌细胞GLC在体外经EMS诱变和阿霉素筛选,诱导出多克隆细胞组成的耐药株,命名为GLC/ADM。两株细胞形态上无明显差异,但GLC/ADM细胞倍增时间由GLC的24.6h增加到30.3h,染色体主流分布由GLC的63～71(64％)左移至59～68(69％)。克隆形成率由49％下降到32％。对阿霉素,长春新碱,足叶乙甙和顺珀的耐受能力分别提高了2.5倍,5.7倍,3.5倍和1.8倍。GLC/ADM显示出多药耐药的特点,提示多种机制的参与,为研究临床耐药性提供了较为理想的细胞模型。     

应用流式细胞术检测同步化处理的肺癌细胞系细胞周期

本文应用吖啶橙酸变性染色方法流式细胞术检测经5-氟脱氧尿嘧啶核苷同步化技术处理的肺癌细胞系GLC-82和HAC-84的细胞周期。结果显示经同步化处理的肿瘤细胞M期比例明显增加,且M期细胞比例与同步化后处理时间密切相关。     

Application of a recombinant replicase to localize the Trionyx sinensis hemorrhagic syndrome virus and evaluate its effects on antiviral genes of T. sinensis

Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV) is an arterivirus newly discovered in Chinese softshell turtles. Little is known about the effect of antibodies against the virus or the distribution of the virus in different organs of infected turtles. In this study, a partial protein of TSHSV-HP4 was produced using a prokaryotic expression system, and its polyclonal antibody was generated. The polyclonal antibody was confirmed by western blot and dot enzyme-linked immunosorbent assay(dot-ELISA). The distribution of TSHSV in different organs of T. sinensis was examined by immunohistochemistry(IHC) and the expression of immune-related genes was analyzed using quantitative real-time polymerase chain reaction(qRT-PCR). The results indicated that the recombinant TSHSV-HP4 protein was successfully expressed, and the generated polyclonal antibody showed specific binding to viral particles in the lung tissues of infected turtles. The IHC assay indicated that the virus was highly localized in various cells, including intestinal lymphocytes,enterocytes, kidney epithelial cells, spleen cells, lung macrophages, and cardiomyocytes. The qRT-PCR analysis revealed that TSHSV was detected in all organs tested, including the lungs, liver, kidneys, spleen, and heart. The numbers of viral mRNA copies in lung and heart tissues were significantly higher in the virus-antibody group than in the virus group. The interferonstimulated genes(ISGs), myxovirus resistance protein 2(MX2) and radical S-adenosyl methionine domain containing 2(RSAD2) were highly upregulated in all groups of infected turtles. Antibody-dependent enhancement(ADE) seemed to occur after stimulation by the polyclonal antibody, because significantly greater expression of the two genes was detected in the virus-antibody group than in the virus group. Overall, these results are important in understanding the cell localization of TSHSV and the immune response of infected turtles.