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1.
Vivek K. Dwivedi Mahesh Chandra P. C. Misra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2005,20(1):21-25
Effect of administration of 600 mg. vitamin E each day, for six days, was observed on activity of some of the anti-oxidant
enzymes and levels of malondialdehyde (as an index of free radical mediated damage) in the platelets of patients reperfused
after myocardial infarction. It has been found that vitamin E administration significantly lowers the level of malondialdehyde
in the patients. Vitamin E administration increases the activities of anti oxidant enzymes (viz. superoxide dismutase, glutathione
reductase and catalase) tested both in the patients and healthy controls. Vitamin E administration causes general stimulation
of anti-oxidant enzyme activities both in healthy persons and the patients, however, lowering of lipid per-oxidation upon
administration of vitamin E is specific for patients. These findings exhibit beneficial role of vitamin E administration in
the management of the patients reperfused after myocardial infarction. 相似文献
2.
Rashmi Raghuvanshi Aiki Kaul Pushpa Bhakuni Aparna Mishra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2007,22(2):90-92
In the present communication, we report remarkably elevated levels of xanthine oxidase activity in the blood of the patients
with myocardial infarction when compared to age and sex matched healthy persons. Highly significant increase of malondialdehyde,
serving as an index of lipid peroxidation and thus free radical mediated damage, has also been found in the patients. We propose
the measurement of the blood levels of xanthine oxidase, a very simple, reliable and less time consuming method as an indicator
of myocardial infarction. 相似文献
3.
Subcutaneous administration of monosodium glutamate (MSG), to normal adult male mice, for six consecutive days at dose levels
of 4 and 8 mg/g body weight, significantly increased the level of free radical initiating enzyme, xanthine oxidase, whereas
the activity of free radical scavenging enzymes, like catalase and superoxide dismutase was significantly decreased in hepatic
tissue. These observations suggested that ingestion of MSG at dose level of 4 mg/g body weight and above, induced oxidative
stress in the hepatic tissue of adult male mice. 相似文献
4.
A. P. Jain A. Mohan O. P. Gupta U. N. Jajoo S. P. Kalantri L. M. Srivastava 《Indian journal of clinical biochemistry : IJCB》1998,13(2):119-122
In view of sufficient body of evidence to substantiate the role of free radical mediated oxidative injury in myocardial infarction
(M.I.), the utility of exogenous supplementation with antioxidant substance, vitamin E was studied. Forty patients of acute
myocardial infarction were divided into two equal groups, one who received vitamin E and the other who did not. Plasma MDA
levels showed a reduction in patients of M.I. who received vitamin E as well as in those who did not, however, the decrease
was significantly more in the vitamin E treated subgroup. 相似文献
5.
Neela Patil Vishwas Chavan N. D. Karnik 《Indian journal of clinical biochemistry : IJCB》2007,22(1):45-51
Free radicals play an important role in the pathogenesis of tissue damage in many clinical disorders, including atherosclerosis.
Antioxidants protect the body from damage caused by free radicals. In this study we investigated oxidative stress, antioxidants
and inflammatory molecules in patients with acute myocardial infarction. This study has been carried out on 106 patients with
acute myocardial infarction, (89 men and 17 females). The control group consisted of 50 healthy, age-matched subjects (40
men and 10 females). Levels of Glucose, lipid profile, glutathione reduced, glutathione peroxidase, Superoxide dismutase,
Glycosylated hemoglobin, fibrinogen, vitamin C, vitamin E, malondialdehyde, ceruloplasmin, adenosine deaminase, lysozyme and
sialic acid were measured. Malondialdehyde and ceruloplasmin levels were significantly high and antioxidants such as vitamin
C, vitamin E, glutathione reduced, glutathione peroxidase and superoxide dismutase were significantly decreased in diabetic
and non-diabetic AMI patients as compared with control (p<0.001). Inflammatory markers showed significant rise in diabetic
patients as compared with controls. Our results clearly show increased inflammation and oxidative stress in patients with
acute myocardial infarction. Depression of antioxidant system in these patients confirms this conclusion. 相似文献
6.
4mg and 8mg monosodium glutamate per gram body weight was administered subcutaneously for 6 consecutive days to normal adult
male mice and its effect was seen on 31st day after the last injection on some antioxidant enzymes in heart. A significant dose dependent increase in lipid peroxidation
and xanthine oxidase level was observed, whereas the activity of free radical scavenging enzymes such as superoxide dismutase
and catalase was decreased in both monosodium glutamate treated groups (Group-2 and Group-3). So, the present work suggested
that monosodium glutamate at dose level of 4mg/g body weight and above induced oxidative stress in the cardiac tissue by changing
the activity of free radical initiating enzyme such as xanthine oxidase and scavenging enzymes like superoxide dismutase and
catalase. 相似文献
7.
K. K. Reddy T. P. K. Reddy B. V. Somasekharaiah K. Soorya Kumarl 《Indian journal of clinical biochemistry : IJCB》1998,13(1):20-26
Multiple mechanisms underlie the human aging process, but interest continues in the role that free radicals and antioxidants
may play. The concentrations of lymphocyte free radical generation (O2
−& H2O2), DNA damage and antioxidant enzyme levels (glutathione Stransferase, superoxide dismutase and catalase) were evaluated in
110 healthy individuals with an age range of 20–80 years. The antioxidant enzyme levels were significantly less in very old
age when compared to young. Moreover, the levels of free radical concentration and DNA damage were increased in the same age
group with respect to younger group. Cigarette smoking had a positive relation with free radicals and DNA damage, and inverse
relation with antioxidants. On the other hand, body mass was found to have positive relation with free radical generation
only. The data indicate that depletion of antioxidant enzyme levels would render the older people more susceptible to free
radical stress and DNA damage. 相似文献
8.
P-selectin, a cell adhesion molecule is elevated in many inflammatory conditions including preeclampsia which is characterized
by generalized endothelial dysfunction and vasoconstriction presumably due to free radicals or mediators released by defective
placentation. Vitamin E has been documented to protect cell membranes from oxidative damage and also decrease platelet aggregation.
The role of vitamin E in pre-eclampsia is contradictory and hence the study was undertaken. Soluble P-selectin was measured
by ELISA and Vitamin-E levels in plasma was estimated spectrofluorometrically. In our study the effect of supplementation
of 400 IU/day of Vitamin E (a-tocopheryl acetate) to patients of pre-eclampsia showed significant decreased levels of soluble
P-selectin by 2nd week as compared to patients given placebo (P = 0.005). In this short period of study no direct correlations were observed between Vitamin E or P-selectin levels with
blood pressure as well as with proteinuria. Future studies may focus on the effect of a-tocopheryl acetate or the phosphate
form of Vitamin-E, recently proposed to be the more active form on other inflammatory markers like IL-6, an important stimuli
of P-selectin release in pre-eclampsia. 相似文献
9.
R. B. Bhogade A. N. Suryakar N. G. Joshi R. Y. Patil 《Indian journal of clinical biochemistry : IJCB》2008,23(3):233-237
Hemodialysis represents a chronic stress status for its recipients. Many hypotheses state that this may be associated with
oxidative stress. Thus, there may be deficiency of antioxidants like erythrocytic superoxide dismutase, catalase, vitamin
E or increased generation of free radicals like superoxide anions. A study was carried out to investigate oxidant and antioxidant
status in chronic renal failure patients undergoing hemodialysis and effect of vitamin E supplementation on these two status.
Blood samples were collected from patients before and after hemodialysis and from controls. The samples were analyzed for
quantitation of MDA as index of lipid peroxide, nitric oxide, vitamin E, vitamin C and enzymatic antioxidants namely erythrocyte
SOD and catalase. As compared to controls, the levels of serum MDA were significantly increased and activities of erythrocyte
SOD and catalase, levels of serum nitric oxide, serum vitamin E and plasma vitamin C were significantly decreased both before
and after hemodialysis. The efficiency of vitamin E therapy in hemodialysis patients was assessed by re-evaluating oxidant
and antioxidant status of same patients after supplementation of vitamin E. Vitamin E supplementation caused decrease in serum
MDA and increase in levels of serum nitric oxide, vitamin E, vitamin C and activities of erythrocytic SOD and catalase. Our
results suggest the presence of oxidative stress and the possible preventive role of vitamin E therapy in hemodialysis patients. 相似文献
10.
The antiulcerogenic effects of the methanolic extract ofSolanum nigrum berries (SBE) on aspirin induced ulceration in rats with respect to antioxidant status in the gastric mucosa have been investigated.
Oxygen free radicals are considered to be important factors in the pathogenesis of gastric ulcer. The level of lipid peroxides,
which were elevated highly in rats with acute gastric mucosal injury was taken as an index of oxidative stress. The activities
of antioxidant defense enzymes were also decreased considerably by oral gastric administration of aspirin. The decreased levels
of antioxidant enzymes and increased mucosal injury were altered to near normal status upon pretreatment with (SBE) when compared
to the ulcer induced rats. The results indicate that (SBE) may exert its gastroprotective effect by a free radical scavenging
action. Our observations suggest that (SBE) may have considerable therapeutic potential in the treatment of gastric diseases. 相似文献
11.
D. Vijayakumar K. Suresh S. Manoharan Ph.D. 《Indian journal of clinical biochemistry : IJCB》2006,21(1):105-105
The main objective of the study was to assess the oxidative stress in plasma and erythrocytes of rheumatoid arthritis patients
by measuring the levels of thiobarbituric acid reactive substances (TBARS), non-enzymatic antioxidants (vitamin E, C and reduced
glutathione) and enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx)]. This
study has been conducted on twenty-two adult female rheumatoid arthritis patients and an equal number of healthy subjects.
Elevated lipid peroxidation and multidirectional changes in the antioxidant defence system were noticed in patients with rheumatoid
arthritis. The enhanced lipid peroxidation accompanied by disturbance in antioxidant status indicates that rheumatoid arthritis
patients are more prone to free radical mediated oxidative damage. 相似文献
12.
Vanishree Bambrana C. D. Dayanand Pushpa Kotur 《Indian journal of clinical biochemistry : IJCB》2017,32(2):171-178
Preeclampsia is a multisystem disorder involves altered homeostasis of oxidants–antioxidants, inflammatory process and endothelial dysfunction. The present study aim was to determine the levels of oxidative stress parameters (malondialdehyde, protein carbonyl, ischemia modified albumin and xanthine oxidase), nutrient antioxidants (vitamin C and vitamin E), enzyme antioxidants (catalase, superoxide dismutase, glutathione peroxidase glutathione reductase), total antioxidant status (TAS) and its association with nitric oxide. The study population consists of three groups, non pregnants (Group 1, n = 57), normotensive pregnants (Group 2, n = 57) and Preeclampsia (Group 3, n = 57). Group 2 and 3 were followed after delivery within 48 h. In preeclampsia xanthine oxidase, malondialdehyde and uric acid levels were significantly increased (p < 0.001), while TAS decreased (p < 0.05) when compared to normotensive pregnant and non pregnant. Catalase, glutathione reductase levels were increased (p < 0.005) and vitamin E, super oxide dismutase levels were decreased (p < 0.001) in preeclampsia when compared to normal pregnants. Receiver operating characteristics curve analysis showed area under curve for xanthine oxidase (0.8), malondialdehyde (0.804), Uric acid (0.84), ischemia modified albumin (0.92) and catalase (0.88) which indicated as good markers in preeclampsia. Amongst, ischemia modified albumin is a better marker of intrauterine hypoxic reperfusion risk with sensitivity 87.7 % and specificity 91.2 %. The increased hydrogen peroxide from xanthine oxidase adds to oxidative stress and increased catalase activity in preeclampsia represents combating action. Increased oxidative stress, decreased TAS and its apparent reversible changes evinced within 48 h after delivery in preeclampsia illustrated that placental abnormality is the contributing factor in the pathogenesis. 相似文献
13.
The excessive generation of reactive oxygen species (ROS) by abnormal spermatozoa and contaminating leukocytes has been defined
as one of the few etiologies for male infertility. Administration of antioxidants in patients with ‘male factor’ infertility
has begun to attract considerable interest. The main difficulty of such an approach is our incomplete understanding of the
role of free radicals in normal and abnormal sperm function leading to male infertility. Mammalian spermatozoa membranes are
very sensitive to free radical induced damage mediated by lipid peroxidation, as they are rich in polyunsaturated fatty acids.
Limited endogenous mechanisms exist to reverse these damages. ROS attacks the fluidity of the sperm plasma membrane and the
integrity of DNA in the sperm nucleus. ROS induced DNA damage accelerate the germ cell apoptosis. Unfortunately spermatozoa
are unable to repair the damage induced by excessive ROS as they lack the cytoplasmic enzymes required to accomplish the repair.
Assessment of such oxidative stress status (OSS) may help in the medical treatment. Treatment strategies must be directed
toward lowering of ROS levels to keep only a small amount necessary to maintain normal cell function. 相似文献
14.
J. O. Olanlokun 《Indian journal of clinical biochemistry : IJCB》2008,23(1):62-66
The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels,
antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated
lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that
oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the
diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while
the level of lipid peroxidation decreased. 相似文献
15.
V. N. Thorat A. N. Suryakar A. S. Sardeshmukh S. S. Sarawade 《Indian journal of clinical biochemistry : IJCB》2004,19(2):32-35
In the present study, the role of serum lipid peroxide and serum nitric oxide as oxidants and erythrocytic superoxide dismutase
& serum vitamin E as antioxidants were determined in the 50 neonates with hypoxic ischaemic encephalopathy. (HIE) as against
25 healthy neonates as controls 50 patients of HIE were further divided into two groups i. e. mild and moderate HIE patients.
All subjects were in the age group of 37–41 weeks of gestation. The levels of serum lipid peroxide, serum nitric oxide and
erythrocytic superoxide dismutase were significantly elevated in both groups of neonates with HIE than those of controls (P<0.001),
whereas serum vitamin E levels were significantly decreased in both groups of HIE patients than those of controls (P<0.001).
A positive correlation was obtained between serum lipid peroxide and erythrocytic superoxide dismutase (r=+0.86). Alterations
in the status of oxidants and antioxidants indicate role of free radicals in the development of HIE. 相似文献
16.
Sajitha GR Jose R Andrews A Ajantha KG Augustine P Augusti KT 《Indian journal of clinical biochemistry : IJCB》2010,25(3):280-288
Daily feeding of drinking water containing lead acetate (160 mg/l) or 10% alcohol by volume or a combination of both to rats
for a month produced certain deleterious effects through oxidative stress. Both heavy metal lead and alcohol are capable of
doing such damages. The deleterious alterations observed were in the parameters of blood, serum and tissues, viz; Hb, Pb,
proteins, lipids, lipid per oxidation, Vitamins C and E levels and enzyme activities of AST, ALT, and catalase. Simultaneous
feeding of either of the two antioxidants garlic oil (GO) and vitamin E at equal doses of 100 mg/kg/day, to the rats counteracted
the deleterious effects of the above two chemicals significantly. The maximum damage was brought about by feeding of drinking
water containing both lead acetate and alcohol. The protective effects of GO and Vitamin E were not significantly different.
The mechanism of actions of the Vitamin E and GO is probably due to their efficiency as detoxifying agents and antioxidants,
to scavenging free radicals as well as an independent action of GO on the removal of lead salt as lead sulfide. 相似文献
17.
Mukesh Nandave Ipseeta Mohanty T. C. Nag Shreesh Kumar Ojha Rajan Mittal Santosh Kumari Dharamvir Singh Arya 《Indian journal of clinical biochemistry : IJCB》2007,22(1):22-28
The present study evaluated the cardioprotective potential of vitamin-E by studying its effect on hemodynamic parameters,
lipid peroxidation, myocyte injury marker and ultrastructural changes in model of isoproterenol-induced myocardial necrosis
in rats. Wistar albino male rats (150–200 g) were randomly divided into saline, ISP control, and vit E groups. Vitamin E group
was administered vitamin E at a dose of 100mg/kg/day while saline and ISP control groups received saline orally for one month.
On 29th and 30th day, ISP (85 mg/kg, sc) was administered at an interval of 24 h to vit E and ISP control rats. On 31st day, rats of all groups were anesthetized and hemodynamic parameters were recorded. At the end of experimentation, animals
were sacrificed; hearts were excised and processed for biochemical and ultrastructural studies. ISP administration produced
marked cardiac necrosis as evidenced by significant decrease in my ocardial creatine kinase-MB as well as increase in malonaldialdehyde
levels. ISP-induced myocardial necrosis resulted in myocardial dysfunction as evidenced by significant depression in heart
rate and mean arterial pressure in the ISP control group as compared to saline control. Salient ultrastructural changes including
extensive loss of myofibrils, muscle necrosis, loss of mitochondria, and formation of several intracytoplasmic vacuoles and
lipid droplets further confirmed the ISP-induced myocardial damage. However, subsequent to ISP challenge, vit E treatment
significantly preserved the myocardium by restoring myocardial CK-MB activity, inhibiting the ISP-induced lipid peroxidation
and ultrastructural changes. Additionally, pre-and co-treatment of vit E prevented the deleterious ultrastructural changes
caused by ISP. These beneficial effects of chronic vit E treatment also translated into significant restoration of the altered
hemodynamic parameters. The present study clearly demonstrated the cardioprotective potential of vit E at dose of 100 mg/kg
in ISP-induced model of myocardial necrosis in rats. The significant restoration of altered hemodynamic parameters, myocardial
CK-MB activity, prevention of ISP-induced rise in lipid peroxidation and ultrastructural changes may confirm its cardioprotective
effect. 相似文献
18.
Free radical generation and lipid peroxidation among the dry cell industry workers exposed to carbon
K. K. Reddy T. P. K. Reddy B. V. Somasekharaiah K. Soorya Kumari 《Indian journal of clinical biochemistry : IJCB》1998,13(1):27-32
A total of 75 healthy male dry cell industry workers exposed to carbon for 5, 8 and 10 years were compared with 48 controls
matched for age and economic status with respect to free radical generation, lipid peroxidation and antioxidant levels. Lymphocytes
were seperated from the whole blood and used for estimation of free radicals and antioxidants. Plasma lipid peroxidation products
were estimated in the form of thiobarbituric acid reactive substances. Significant increase in free radical generation and
lipid peroxidation products were observed in carbon exposed population than controls, and the increase was found to be significant
with increase in the period of exposure. The levels of antioxidants i.e., superoxide dismutase (SOD) and catalase were found
to decrease with the increase in exposure to carbon in industrial workers. These results suggest that exposure to carbon augments
free radical generation, lipid peroxidation and promotes decline in antioxidant enzymes. 相似文献
19.
Damodara Reddy V Padmavathi P Gopi S Paramahamsa M Varadacharyulu NCh 《Indian journal of clinical biochemistry : IJCB》2010,25(4):419-424
The effect of Emblica officinalis fruit extract (EFE) against alcohol-induced hepatic damage in rats was investigated in the present study. In vitro studies showed that EFE possesses antioxidant as well nitric oxide (NO) scavenging activity. In vivo administration of alcohol (5 g/kg b.wt/day) for 60 days resulted increased liver lipid peroxidation, protein carbonyls, nitrite plus nitrate levels. Alcohol administration also significantly lowers the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and reduced glutathione as compared with control rats. Administration of EFE (250 mg/kg body weight) to alcoholic rats significantly brought the plasma enzymes towards near normal level and also significantly reduced the levels of lipid peroxidation, protein carbonyls and restored the enzymic and non-enzymatic antioxidants level. This observation was supplemented by histopathological examination in liver. Our data indicate that the tannoid, flavonoid and NO scavenging compounds present in EFE may offer protection against free radical mediated oxidative stress in rat hepatocytes of animals with alcohol-induced liver injury. 相似文献
20.
Ramesh Chander Farhan Rizvi A. K Khanna Ram Pratap 《Indian journal of clinical biochemistry : IJCB》2003,18(2):71-79
Guggulsterone, a mixture of cis (E) and trans (Z) isomers (7∶3 w/w) was synthesized from 16-DPA. The isomers were separated
by column chromatography and evaluated for cardioprotective and antioxidant activities. Myocardial necrosis induced by isoproterenol
in rats caused marked increase in serum creatine phosphokinase and glutamate pyruvate transaminase. Simultaneously in ischemic
heart, phospholipase, xanthine oxidase and lipid peroxides were enhanced following depletion of glycogen, phospholipids and
cholesterol. Treatment with guggulsterone and its both isomers at the dose of 50 mg/kg po., significantly protected cardiac
damage as assessed by the reversal of blood and heart biochemical parameters in ischemic rats. The cardioprotective activity
of guggulsterone and of both the isomers were compared with that of gemfibrozil at the same doses. Guggulsterone and both
the isomers at tested concentrations (5–20mM) inhibited oxidative degradation of lipids in human low-density lipoprotein and
rat liver microsomes induced by metal ionsin vitro. The drug counteracted against the generation of superoxide anions (O2) and hydroxyl radicals (OH−) in non-enzymic test systems. It is suggested that cardioprotective and antioxidant activities of synthetic guggulsterone
and guggulsterone obtained from gum resinCommiphora mukul that contains isomers E & Z in the ratio of 46∶54w/w are the same. 相似文献