Nucleocapsid protein from porcine epidemic diarrhea virus isolates can antagonize interferon-λ production by blocking the nuclear factor-κB nuclear translocation

Porcine epidemic diarrhea virus (PEDV) is a highly infectious pathogen that can cause severe diseases in pigs and result in enormous economic losses in the worldwide swine industry. Previous studies revealed that PEDV exhibits an obvious capacity for modulating interferon (IFN) signaling or expression. The newly discovered type III IFN, which plays a crucial role in antiviral immunity, has strong antiviral activity against PEDV proliferation in IPEC-J2 cells. In this study, we aimed to investigate the effect of PEDV nucleocapsid (N) protein on type III IFN-λ. We found that the N proteins of ten PEDV strains isolated between 2013 and 2017 from different local farms shared high nucleotide identities, while the N protein of the CV777 vaccine strain formed a monophyletic branch in the phylogenetic tree. The N protein of the epidemic strain could antagonize type III IFN, but not type I or type II IFN expression induced by polyinosinic-polycytidylic acid (poly(I:C)) in IPEC-J2 cells. Subsequently, we demonstrated that the inhibition of poly(I:C)- induced IFN-λ3 production by PEDV N protein was dependent on the blocking of nuclear factor-κB (NF-κB) nuclear translocation. These findings might help increase understanding of the pathogenesis of PEDV and its mechanisms for evading the host immune response.     

Chaos from jerk circuit

We present an experimental circuit realization of a simple jerk equation with modulus nonlinearity and study the chaotic behaviour as a function of a variable control parameter. The third order differential equation has been realized using commonly available opamps and the nonlinearity using a pair of diodes. The experiments indicate that chaotic behaviour indeed emerges through the period doubling route as the parameter is changed. This work is instructive to show students the phase plots as a function of the control parameter and determine the Feigenbaum constant.     

网络流量异常检测

提出了一种新颖的网络洪流攻击的异常检测机制.这种检测机制的无状态维护、低计算代价的特性保证了自身具有抗洪流攻击的能力.本文以检测SYN洪流攻击为实例详细阐述了异常检测机制.这个机制应用EWMA方法检测网络流的突变, 并运用对称性分析方法检测网络流的异常活动.测试结果表明本文所提出的检测机制具有很好的检测洪流攻击的准确度, 并具有低延时特性.     

EWMA算法在无线通信协议速率选择中的应用

如何根据无线环境的变化实时选择一个可靠的发送速率,进而保证数据传输的可靠性是无线通信研究的热点。针对传统累积和平均法（Cumulative Sum Average,CUSUMA）由于计算机存储数据位数的有限性可能导致的数据值超过计算机最大数据类型表示范围,进而造成统计数据丢失的问题,提出基于指数加权移动平均（Exponentially Weighted Moving Average, EWMA）算法,计算对应速率下的数据发送成功率,并根据计算出的成功率实现动态速率选择。该算法可用于解决通用平均值算法导致的因数据过大造成的统计数据丢失问题。     

用修正系数法构造θ~2的无偏估计

在总体分布为指数分布和均匀分布时,由未知参数θ的无偏估计量,通过修正系数法直接构造θ2的无偏估计量.     

Relative Performance of Categorical Diagonally Weighted Least Squares and Robust Maximum Likelihood Estimation

Robust maximum likelihood (ML) and categorical diagonally weighted least squares (cat-DWLS) estimation have both been proposed for use with categorized and nonnormally distributed data. This study compares results from the 2 methods in terms of parameter estimate and standard error bias, power, and Type I error control, with unadjusted ML and WLS estimation methods included for purposes of comparison. Conditions manipulated include model misspecification, level of asymmetry, level and categorization, sample size, and type and size of the model. Results indicate that cat-DWLS estimation method results in the least parameter estimate and standard error bias under the majority of conditions studied. Cat-DWLS parameter estimates and standard errors were generally the least affected by model misspecification of the estimation methods studied. Robust ML also performed well, yielding relatively unbiased parameter estimates and standard errors. However, both cat-DWLS and robust ML resulted in low power under conditions of high data asymmetry, small sample sizes, and mild model misspecification. For more optimal conditions, power for these estimators was adequate.     

MATCH（27，3，1）－设计

一个MATCH（n,k,λ）－设计就是完全图kn的一个k－匹配集合,使得kn中的每一对独立边恰好出现在λ个k－匹配中．本文利用拉丁方完备化方法构作一个MATCH（27,3,1）－设计。     

关于图的瑕边着色

图G=(V,E)的一个(λ,β)-瑕k-边着色是一个从E到{1,2,…,k}的映射,且存在一个最小整数β≥1,对每一个色j∈{1,2,…,β},至少存在一个顶点uj∈V(G)使得顶点uj关联着有色的j条边;对每一个色l∈{β+1,…,k},没有两条相邻边着有色l.图G的(λ,β)-瑕色数被表示为χ(λ,β)(G),它是一个最小的整数,使对整数k≥χ(λ,(β)G),图G总有一个(λ,β)-瑕k-边着色.在这篇文章中,我们证得χ(λ,1)(G)+λ-1≤χ′(G)≤χ(λ,1)(G)+,其中χ′(G)是G的正常边色数,并确定了几个特殊图类的瑕色数.     

Control design of 60 kW PEMFC generation system for residential applications

This paper presented a control design methodology for a proton exchange membrane fuel cell （PEMFC） generation system for residential applications. The dynamic behavior of the generation system is complex in such applications. A compre- hensive control design is very important for achieving a steady system operation and efficiency. The control strategy for a 60 kW generation system was proposed and tested based on the system dynamic model. A two-variable single neuron proportional-integral （PI） decoupling controller was developed for anode pressure and humidity by adjusting the hydrogen flow and water injection. A similar controller was developed for cathode pressure and humidity by adjusting the exhaust flow and water injection. The desired oxygen excess ratio was kept by a feedback controller based on the load current. An optimal seeking con- troller was used to trace the unique optimal power point. Two negative feedback controllers were used to provide AC power and a suitable voltage for residential loads by a power conditioning unit. Control simulation tests showed that 60 kW PEMFC generation system responded well for computer-simulated step changes in the load power demand. This control methodology for a 60 kW PEMFC generation system would be a competitive solution for system level designs such as parameter design, performance analysis, and online optimization.     

带参数的奇异三阶三点边值问题的正解

本文讨论下述带参数的奇异三阶三点边值问题■其中γ>0是参数且a(t)在t=0和t=1处具有奇性,当f和a满足适当条件时,对一定取值范围内的γ,获得了上述边值问题正解的存在性与不存在性.所用主要工具是Guo-Krasnoselskii不动点定理.     

C~n空间中有界域一个积分公式的拓广

利用向量函数组w1,w2, ...wm-1以及互相独立的实参数组λ1,λ2, ...λm-1证明了Cn空间有界域上光滑函数的积分表示公式，这个公式可以看为全纯域上著名积分公式在光滑函数上的拓广，通过适当选择其中的向量函数组和参数组可以得到Cn空间各种有界域上的积分表示公式。     

一个核含对数函数的Hilbert型积分不等式

应用实分析、复分析技巧及权函数方法,建立一个新的具有最佳常数因子的核为对数函数ln(1+bxδλyλ/1+axδλyλ)(λ0,0ab,δ∈{-1,1})的Hilbert型积分不等式,还导出了其等价式及特殊参数δ=-1下的齐次形式.     

伴发ATTR型淀粉样沉积的骨关节炎患者的临床和生物学特征分析(英文)简

Objective： Our aim was to investigate clinical and laboratory characteristics of osteoarthritic patients who had amyloid deposition in their knee joints. Methods： Synovial membranes were obtained from 36 patients with knee osteoarthritis （OA） who underwent joint replacement surgery. From this sample, the diagnosis of amyloid was deter- mined by Congo red staining, which demonstrated apple-green birefringence under a polarized microscope. All syn- ovial membranes were immunohistochemically characterized for the expressions of amyloid immunoglobulin light chain （AL-K and AL-,k）, serum amyloid-A （SAA）, amyloidogenic transthyretin （ATTR）, and amyloidogenic 152- microglobulin （A152M）. Matrix-assisted laser desorption-ionizaton/time of flight mass spectrometry （MALDI-TOF MS） was used to analyze transthyretin （TTR） isoforms in the serum of each patient. Results： Nine cases （25%） were found to be amyloid-positive. Immunohistochemicaliy, eight cases （88.9%） had ATTR deposition, and one sample （11.1%） was shown to be AL-K-positive. MALDI-TOF MS identified that the TTR in the serum of the patients was unmodified wild-type TTR, TTR-Cys-S-S-Cys, and TTR-Cys-S-S-CysGly. The age at surgery and the disease duration were sig- nificantly higher in the ATTR-positive group than in the ATTR-negative group. Knee score and function score were significantly lower in the ATTR-positive group than in the ATTR-negative group. Conclusions： Amyloid deposition in synovial membranes of OA patients was found to be ATTR and AL-K. TTR in the serum of the patients was unmodified wild-type TTR together with two isoforms. The high age at surgery, long disease duration, and a deteriorated knee function were associated with ATTR amyloid deposition in the osteoarthritic knee joints.     

代数Riccati方程数值解的一种牛顿迭代法

代数Riccati方程在优化控制理论中具有十分重要的作用.结合了二次方程的牛顿迭代法与Lya-punov方程的自由参数轮转方向迭代法,提出了一种求代数Riccati方程数值解的一种新方法,并给出了算法的收敛性证明.最后,给出了具体的数值算例.     

广义d_m-齐对虾树的(λ,μ)-魔幻标号

图标号问题来自错综复杂的编码领域、确定晶体学中X-射线的二分性、通信网络设计中的寻址问题、确定最佳电路布局和射电天文等方面.本文推广对虾树到广义dm-齐对虾树,并研究了这类树的(λ,μ)-魔幻标号.     

Gender differences in cognitive abilities of reading-disabled twins

Gender differences were assessed in three research-identified samples of children who were members of twin pairs: (1) 120 male and 124 female probands from same-sex identical and fraternal twin pairs in which at least one member of each pair is reading disabled; (2) a comparison sample of 148 males and 161 females from same-sex twin pairs with no history of reading problems; and (3) 34 pairs of opposite-sex fraternal twin pairs in which at least one member of each pair is reading disabled. Results of multivariate analyses of variance of psychometric test data from the two samples of same-sex twin pairs, in which the male and female subjects were reared in different homes, suggest that profiles of gender differences are similar in reading-disabled and control children. Moreover, this pattern of gender differences also tended to occur in opposite-sex twin pairs, who shared prenatal, as well as early postnatal, environmental influences. In general, reading-disabled males obtained higher average scores than affected females on Wechsler (1974, 1981) Verbal and Performance IQ, but lower scores on Reading Recognition and Spelling subtests of the Peabody Individual Achievement Test (Dunn and Markwardt 1970). With regard to the Wechsler subtests, reading-disabled males achieved higher average scores on Information, Picture Completion, Block Design, and Object Assembly, but lower scores on Coding. Although significant and relatively consistent gender differences in cognitive measures were found in three samples included in this study, these differences account for only a small portion of the observed variance. This work was supported in part by a program project grant from NICHD (HD-11681), and the report was prepared while J.J. Gillis was supported by NICHD training grant HD-07289.     

Vibration Control Induced by Ice of a Jacket Platform

Thevibrationinducedbyiceisanimportantfactorofdamageforjacketplatforms ,sotheiceloadshavebeenthecontrolloadforjacketplatformsintheBohaiBay[1] .Toreducedamageinducedbytheiceforce ,theloadsstandardofjacketplatformshasbeenimproved .Infact ,thestrengthofjacketplatformsagainststaticiceforceismeteasily .But,thesafetyproblemoftheplatformaf fectedbyboththebehaviorsofself excitedandcouplingissolvedbyothertechnologysolutions[2— 3] .Xuetalde signedasetofdevicesforreducingvibrationofthejacketplatforminth…     

Intratrial proactive interference in rats’ serial alternation performance in the radial maze

Rats acquired a serial alternation task in an eight-arm radial maze that was partitioned into four pairs of arms. Each pair was associated with a different distal stimulus. Rats were initially forced to the left or right arm in each pair (the study segment) before being exposed to both arms in each pair (the free-choice or test segment). Only the previously blocked arm of each pair remained baited. Following initial training, proactive interference (PI) was induced by presenting rats with a forced-choice (prestudy) segment containing arm positions opposite those in the subsequent study segment. Such trials generated poorer free-choice accuracy than did trials without a prestudy segment. Forcing rats to both arms in the pair in a prestudy segment produced only transient PI. A slight improvement in rats’ free-choice performance was obtained by forcing them to the same arm position, but only when the test segment was delayed by 30 min. Increasing the interval between the prestudy and study segments from 2 to 30 min eliminated PI, but only when free-choice testing was delayed by 2 min rather than by 30 min. These results suggest that intratrial PI in this preparation was primarily due to confusion about which arm position in each pair had been visited during the last forced-choice segment.     

Exploration of Factors Affecting the Added Value of Test Subscores

Recently, interest in test subscore reporting for diagnosis purposes has been growing rapidly. The two simulation studies here examined factors (sample size, number of subscales, correlation between subscales, and three factors affecting subscore reliability: number of items per subscale, item parameter distribution, and data generating model) that affected the value of reporting subscores within the classical test theory framework. Results showed that a higher proportion of subscores of added value was related to lower correlation between subscales, more items per subscale, no guessing in responses, smaller variability in difficulty parameters, and matched average item difficulty and average examinee ability.     

Acquired equivalence of cues in pigeon autoshaping: Effects of training with common consequences and with common antecedents

In three experiments, we sought evidence for the acquired equivalence of cues in pigeons trained in an autoshaping paradigm. In Experiment 1, presentations of each of a pair of cues (different keylight stimuli) preceded a common consequence (a different keylight stimulus). The pattern of response then established by further training given to one member of the pair was found to generalize preferentially to the other, demonstrating equivalence between cues that had shared a common consequence. The same test procedure was used in Experiment 2, but with a training procedure in which each cue of a pair was preceded by a given stimulus. This too resulted in enhanced generalization between members of the pair, showing that equivalence can be established when cues have been experienced along with a common antecedent. Both training procedures were combined in Experiment 3 to confirm the reliability of the effects previously obtained. The discussion is focused on ways in which the associative explanation offered for cases of equivalence mediated by a common consequence might be extended to accommodate equivalence mediated by a common antecedent.