生物技术在番木瓜上的应用

综述生物技术在番木瓜上应用,包括番木瓜离体快繁、胚培养、体细胞胚诱导、制作鼢人工种子、分子标记与基因工程等方面的内容.     

生物技术在番木瓜上的应用

综述生物技术在番木瓜上应用，包括番木瓜离体快繁、胚培养、体细胞胚诱导、制作人工种子、分子标记与基因工程等方面的内容。     

番木瓜花芽分化期内源多胺的变化

对番木瓜花芽分化过程中内源多胺进行测定,结果表明:在番木瓜花芽的生理分化期,番未瓜叶片中的腐胺(Put)、精胺(Spm)和亚精胺(Spd)含量上升,出现积累高峰;于形态分化期下降.番木瓜花芽分化期3种内源多胺不同程度的累积对成花的诱导有促进作用,3种内源多胺特别是Spd与花芽分化之间存在着内在的联系.     

果树病毒病的防治

本文讨论了柑桔、苹果枣、葡萄、番木瓜等果树病毒病的种类及防治措施．     

瓜类大家庭——葫芦科植物漫话

提起瓜类,大家并不陌生,如西瓜、黄瓜、丝瓜、南瓜、冬瓜、哈蜜瓜、黄金瓜等等,这些瓜类在植物分类学中,都属于葫芦科植物。但是并非所有称为某某瓜的都是葫芦科植物,如木瓜是蔷薇科植物,番木瓜是番木瓜科植物。葫芦科植物经济价值很大,与人类关系密切。我国瓜类栽培,历史悠久,唐代诗人王建诗云:     

番木瓜综合利用的研究

介绍热带名果番木瓜的历史、现状、营养及综合利用。阐述了番木瓜蛋白酶、果脯、果酱等制品的工艺流程。     

番木瓜综合利用的研究

介绍热带名果番木瓜的历史、现状、营养及综合利用.阐述了番木瓜蛋白酶、果脯、果酱等制品的工艺流程.     

食肉人工嫩化与嫩肉粉的研究

本文介绍了食肉人工嫩化的历史,番木瓜蛋白酶的利用,嫩肉粉的研制和使用。阐述酶在肉类加工方面应用的前景。     

食肉人工嫩化与嫩肉粉的研究

本介绍了食肉人工嫩化的历史，番木瓜蛋白酶的利用，嫩肉粉的研制和使用。阐述酶在肉类加工方面应用的前景。     

可以治小病的十种水果

头发染发烫发——鳄梨染发烫发过程会夺走头发的水分和油脂,头发变得干枯。成熟的鳄梨中含有30%的珍贵植物油脂——油酸,对干枯的头发有特殊功效。大脑过度用脑——香蕉过度用脑导致人体内维生素、矿物质及热量缺乏,除了大脑疲惫,还常常感到情绪低落。此时补充香蕉可提供所需营养物质并缓解消极情绪。眼睛过度用眼——番木瓜长时间盯着电脑屏幕或电视屏幕,过度用眼,则视网膜感光所依靠的关键物质维生素A大量消耗,眼睛感到干燥、疼痛、怕光,甚至视力下降。此时就需要食用可提供大量维生素A的番木瓜。牙龈牙龈出血——猕猴桃牙龈健康与维生素…     

水平基因转移与物种演化

遗传学、基因组学和生物信息学业已证明，由病毒、类病毒、质粒等介导的细胞、个体和物种之间的水平基因转移广泛地存在于细菌、古生菌和真核生物中，水平基因转移可能造成同一生境中物种分化的抑制，种群的快速协同进化，不同物种的趋同进化、返祖遗传、获得性状(基因)遗传和家族成员的性状趋同等。因此，水平基因转移是物种进化的一个重要驱动力。     

基因探针技术检测血清1型马立克氏病病毒的研究

选用马立克氏病病毒（MDV）GA株BamHI基因文库中LDNA片段，制成DIG—标记的MDV核酸探针，分别对1型MDV强毒株（BJ-1株）和弱毒株（Md11／75c株）感染材料的核酸进行dotblot杂交检测，结果显示均有阳性呈色反应；而对MDV血清2型（SB-1株）、3型（Fe126株）及禽网状内皮组织增殖病病毒（REV）和淋巴细胞性白血病病毒（LLV）感染细胞的核酸，作上述同样检测，则均未见任何颜色显现。     

RNAi的研究及应用

RNA干扰（RNA interference,RNAi）是由双链RNA（double-stranded RNA,dsRNA）引发的转录后基因沉默机制．RNAi可以调节和关闭基因的表达,进而调控细胞的各种高级生命活动,是真核生物中普遍存在的抵抗病毒入侵、抑制转座子活动、调控基因表达的监控机制．目前RNAi的研究取得了很大进展,有可能为肿瘤基因治疗提供新策略．     

Construction and identification of the recombinant of the AAV vector and human interferon-gamma

To construct and identify further a recombinant of Adeno-associated virus and interferon-gamma for gene therapy, the full-length IFN-γcDNA containing signal peptide was amplified by PCR, and then cloned into the pUC18. After screening, the fragment from the positive clone was then subcloned into pwp19. After the correct recombinant was identified by digestion with SacI and BamHI, it was transfected into lymphocyte cell line H9 mediated by calcium phosphate, and the expression of IFN-γ was detected by RT-PCR and ELISA. The result showed that the IFN-γ were expressed in the H9 cells transfected with pwp/IFN-γ. The so constructed recombinant plasmid pwp19/IFN-γ containing the full-length IFN-γ gene was expressed in mammalian cells. Project (39570653) supported by NFSC.     

鸡新城疫病毒ND-xx08株F基因的克隆及分析

新城疫病毒(NDV)ND-xx08毒株经10 d龄SPF鸡胚增殖后,提取其基因组RNA并反转录成cDNA,用NDV F基因特异性引物,经PCR扩增后获得与F基因预期大小一致的DNA片段。将NDV F基因片段克隆到pMD18-T载体上,并进行EcoR I和Hind III双酶切鉴定和测序鉴定。结果显示,ND-xx08毒株F基因片段的长度为1 662 bp,共编码554个氨基酸,F蛋白的裂解位点为112R-R-Q-K-R-F117,是典型强毒株氨基酸序列结构。将NDV ND-xx08株F基因的47 bp到420 bp序列与新城疫病毒基因型I至基因型Ⅸ毒株的相同序列绘制病毒基因进化树,显示ND-xx08分离株属于基因Ⅶe型。将NDV ND-xx08株F全基因与国内外发表的23株NDV F基因核苷酸序列和氨基酸序列的同源性比较分析,结果表明,其核苷酸序列的同源性在82.7%~97.8%之间,氨基酸同源性在87.5%~97.7%之间。     

A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana

The AtTOM1 gene of Arabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus(TMV) in A.thaliana.In this study,we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1.Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N.tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities,respectively.Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N.benthamiana.The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants.     

家蚕核型多角体病毒p35基因的核苷酸序列分析

对家蚕核型多角体病毒苏州株（ＢｍＮＰＶｓｕ）ｐ３５基因的序列分析表明：ＢｍＮＰＶｓｕ ｐ３５编码序列为８９７ｍｔ,编码２９８ａａ。同源性分析表明：ＢｍＮＰＶｓｕ ｐ３５与ＢｍＮＰＶＴ３、ＡｃＮＰＶ 、ＳＩＮＰＶ在核苷酸水平上同源性分别为９９．５％、９５．１％、８９．３％,在氨基酸水平上的 性分别为９８．７％、８９．４％、７６．４％,显示了杆状病毒ｐ３５基因在进化上的保守性。ＢｍＮＰＶＴ３中位的Ｎ１     

Science Letters:Assignment of CCR7 gene to chicken chromosome 27 by radiation hybrid panel mapping

The protein encoded by CC chemokine receptor 7 (CCR7) is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6 000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 (6 cR away) with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH (radiation hydrid) map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene.     

Assignment of <Emphasis Type="Italic">CCR7</Emphasis> gene to chicken chromosome 27 by radiation hybrid panel mapping

The protein encoded by CC chemokine receptor 7 （CCR7） is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus （EBV）, and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel （ChickRH6） was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 （6 cR away） with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH （radiation hydrid） map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene.