弓形虫感染小鼠尿液中弓形虫循环抗原的检测

目的：探索弓形虫感染早期诊断的快速简便方法。方法：用快速ELISA检测弓形虫轻度、中度和重度感染小鼠尿中弓形虫循环抗原（TCA）。结果：在轻、中、重3个感染组鼠尿中均检出TCA。结论：快速ELISA是一种特异、敏感的弓形虫循环抗原检测方法,可用于弓形虫感染的早期诊断。     

河北省大学生弓形虫感染情况调查

目的:为了解河北省大学生弓形虫感染情况。方法:我们应用间接血凝试验(IHA)对河北北方学院1280名新生进行了弓形虫感染情况调查。结果:职业家庭同学感染率高于非职业家庭同学(P<0.05);男、女同学间感染率无显著性差异;张家口、承德、保定地区同学感染率较高;养猫狗家庭同学感染率高于无猫狗家庭同学,农村养猫狗同学感染率高于城市养猫狗同学。结论:河北省不同地区同学均有弓型虫感染,提示我们应加强饮食及个人卫生,同时应提醒广大医务人员加强对此病的认识。     

癫痫与弓形虫感染的关系研究

目的探讨癫痫发病与弓形虫感染的关系。方法用ELISA法检测癫痫患者(患者组)28例和健康人(对照组)46例的血清弓形虫IgG、IgM抗体及循环抗原(CAg),阳性者为弓形虫感染。结果患者组弓形虫感染率为21.43%,其中1例儿童患者IgG和IgM抗体双阳性;对照组仅检出IgG抗体,感染率为26.09%,两组中CAg均未检出。两组感染率比较无显著性差异(P>0.05)。结论癫痫的发病与弓形虫感染的关系不大,但少数癫痫患者需排除弓形虫脑病。     

精神分裂症患者弓形虫感染的血清学调查

目的 :了解张家口地区精神分裂症患者弓形虫感染状况 ,探讨弓形虫感染与精神分裂症之间的关系。方法 :采用间接红细胞凝集试验对 2 2 2例住院精神分裂症患者及 78例健康体检者进行弓形虫抗体检测。结果 :张家口地区精神分裂症患者弓形虫抗体阳性率为 7.6 6 % ,健康体检者为 1.2 8% ,两者之间差异显著 (P <0 .0 5 )。结论 :本地区精神分裂症患者弓形虫抗体阳性率高于健康人群。提示临床医务工作者应加强对弓形虫病的认识 ,重视精神病患者的弓形虫感染情况 ,对门诊和住院病人进行常规弓形虫检测。     

肿瘤患者弓形虫抗体调查

本文对178例肿瘤患者和238例健康人,采用间接血凝试验(IHA)进行弓形虫抗体检测。结果患者组阳性27例,感染率为15.16％;健康人阳性者7人,感染率为2.94％。     

某市孕前妇女ToRCH感染情况分析

目的 :了解某市孕前妇女ToRCH感染情况并指导有计划地妊娠 .方法 :用酶联免疫吸附法(ELISA)检测 1 791例孕前妇女血清中ToRCH_IgM并对结果进行统计分析 .结果 :某市孕前妇女弓形体、风疹病毒、巨细胞病毒、单纯疱疹病毒Ⅱ型的感染率分别为 3 8%、2 0 %、2 3%、2 5 % ,农村感染率高于城市 ,两组差异有显著性 (P <0 0 5) .结论 :某市孕前妇女ToRCH感染率与国内其它地区报道相近 ,孕前ToRCH筛查对指导妊娠、优生优育非常必要     

三种不同包被抗原用于RF的测定与比较

分别用人、兔、鼠IgG作包被抗原,检测244份血清标本(类风湿性关节炎72份,系统性红斑狼疮74份,慢性肾病50份,其它内科疾病33份,健康体检者15份)。类风湿因子RF,发现以AgHIgG作为包被抗原时,方法最敏感,AgRIgG次之,而AgMIgG最差。以AgRIgG为包被抗原(间接法)与以AgHIgG、AgRIgG为包被抗原(双抗原夹心法)所测RF有较好一致性,P分别为0.01,P<0.05,P<0.01。     

猪瘟与蓝耳病混合感染的诊断与治疗方法

通过流行病学调查、临床表现、病理剖检对新乡市长垣县赵堤镇某猪场发生的一起以体温升高、不食、腹泻、耳部发绀、鼻部、腹下、臀部、四肢末端发紫为特征的疾病,做出初步诊断为猪瘟与蓝耳病混合感染,然后采取10份病料经处理后用正向间接血凝试验检测CSF,酶联免疫吸附试验检测PRRS,结果表明CSF的阳性率为80%,PRRS的阳性率为60%,从而确诊该病为CSF与PRRS混合感染,通过采取自家苗、抗猪瘟血清、接种疫苗三种方法作比较,同时肌肉注射杂症一针,菌毒急救,拌料饲喂防蓝灵,金方案Ⅱ型,饮水中添加口服补液盐,电解多维等综合防治措施,结果显示自家苗治疗效果最好,病情得到控制,治愈率达70%,为目前规模化猪场混合感染的快速检测和治疗提供了可参考性的建议.     

急性脑梗死患者血清TNF-α变化

为了研究急性脑梗死患者血清中肿瘤坏死因子-α(TNF-α)含量的变化,我们采用病例对照的研究方法,选择急性脑梗死患者为病例组,健康体检者为对照组,用酶联免疫吸附法定量检测血清中TNF-α的含量,现报告如下:1资料与方法1·1一般资料病例组选取2008·1~2008·6月在我院住院急性脑     

皖南地区规模场猪伪狂犬病血清学分析

针对近年来严重影响规模猪场的传染性疾病猪伪狂犬病,采集了皖南地区31个不同规模种猪场血清样品1000份,使用酶联免疫吸附试验检测法,进行猪伪狂犬病野毒感染抗体血清学调查。结果：31个种猪场中,21个种猪场gE抗体阳性,阳性率67．74％;检测种猪血清1000份,gE抗体阳性率为23％。皖南地区种猪场均有PR感染情况,其中青阳县PR野毒抗体阳性率最高;150头以下的猪场,抗体阳性率较高。分析结果为皖南地区规模猪场制订PR综合防控措施提供了依据。     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｔｈｅｆｉｒｓｔｓｕｃｃｅｓｓｆｕｌｉｓｏｌａｔｉｏｎｏｆＨ .ｐｙ ｌｏｒｉｉｎ 1 982 ,Ｈ .ｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎｈａｓｂｅｅｎｆｏｕｎｄｔｏｂｅａｓｓｏｃｉａｔｅｄｗｉｔｈｖａｒｉｏｕｓｄｉｓｅａｓｅｓｉｎｃｌｕｄｉｎｇｃｈｒｏｎｉｃｇａｓｔｒｉｔｉｓ,ｐｅｐｔｉｃｕｌｃｅｒｄｉｓｅａｓｅｓ(Ｄｏｏｌｅｙｅｔａｌ.,1 989;Ｇｒａｈａｍｅｔａｌ.,1 992 ) ,ａｎｄｇａｓｔｒｉｃｎｅｏｐｌａｓｍｓ(Ｂａｙｅｒｄｏｒｆｆｅｒｅｔａｌ.,1 995) .Ｉｔｗａｓｓｕｇｇｅｓｔ…     

Development and evaluation of immunoassay for zeranol in bovine urine

A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1：5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation （CVs） were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol （ranging from 0.2 to 10 μg/ml） were detected by the ELISA and liquid chromatography （LC） method, and good correlations were obtained between the two methods （R^2=0.9643）. We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine.     

两系不育系早S花粉育性的世代分布

通过早S连续两个世代花粉染黑率的观测,证实群体育性呈正态分布。由于变异和自然选择压,群体后代育性趋向提高,频率分布趋向分散,从而提高“杂株”频率。     

Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii

The dense granule protein 4 (GRA4) is a granular protein from Toxoplasma gondii, and is a candidate for vaccination against this parasite. In this study, the plasmid pcDNA3. 1-GRA4 (pGRA4), encoding for the GRA4 antigen, was incorporated by the dehydration-rehydration method into liposomes composed of 16 mmol/L egg phosphatidylcholine (PC), 8 mmol/L dioleoyl phosphatidylethanolamine (DOPE), and 4 mmol/L 1,2-diodeoyl-3-(trimethylammonium) propane (DOTAP). C57BL/6 mice and BALB/c mice were immunized intramuscularly three times with liposome-encapsulated pGRA4 to determine whether DNA immunization could elicit a protective immune response to T. gondii. Enzyme-linked immunosorbent assay (ELISA) of sera from immunized mice showed that liposome-encapsulated pGRA4 generated high levels of IgG antibodies to GRA4. Production of primary interferon (IFN)-γ and interleukin (IL)-2 in GRA4-stimulated splenocytes from vaccinated mice suggested a modulated Th1-type response. 72.7% of C57BL/6 mice immunized with liposome-encapsulated pGRA4 survived the challenge with 80 tissue cysts of ME49 strain, whereas C57BL/6 mice immunized with pGRA4 had only a survival rate of 54.5%. When immunized BALB/c mice were intraperitoneally challenged with 103 tachyzoites of the highly virulent RH strain, the survival time of mice immunized with liposome-encapsulated pGRA4 was markedly longer than that of other groups. Our observations show that liposome-encapsulated pGRA4 enhanced the protective effect against infection of T. gondii.     

辣椒质-核互作雄性不育与叶、蕾中内源激素含量的关系

利用间接酶联免疫法(ELISA)测定辣椒质核互作雄性不育三系叶、蕾中IAA、CTKs、GA1+3和ABA的含量.结果表明:在叶、蕾中各种内源激素水平三系之间有明显的差异.在三系之间不育系叶、蕾中IAA的含量明显增高;叶片中CTKs的含量不育系最高,而在花蕾中其含量最低;在花蕾中不育系GA1+3的含量明显最高;ABA的含量三系之间差异相对较小.进定步分析表明;花蕾中各种激素水平的比例平衡与辣椒雄性不育有着更密切的关系.     

重组猪囊尾蚴cC1蛋白用于囊尾蚴病人血清抗体的检测

诱导、表达含有猪囊尾蚴cC1蛋白基因的原核表达载体pET-28a质粒(pET28a-cC1),纯化、鉴定融合蛋白,以此作为抗原包被反应板,对囊尾蚴病人等血清进行ELISA检测。检测56份囊尾蚴病患者血清,抗体阳性者53份,阳性率为94.7％;检测30份血吸虫病患者血清,交叉反应率为3.33％(1/30);检测30份正常...     

张家口市SARS患者、疑似病例、留观及密切接触医护人员血清中IgG、IgM抗体测定

目的 :为临床诊断及排除严重急性呼吸综合征 (SARS)提供依据和对留观及密切接触医护人员有无感染作初步调查。方法 :用酶联免疫吸附测定 (ELISA) 5 3例临床诊断病例、2 9例疑似病例、6 3例留观及 114例密切接触医护人员的血清中SARS冠状病毒IgG、IgM抗体。结果 :SARS临床诊断病例IgG抗体阳性 2 1例 ,IgM抗体阳性 18例 ,其中有 17例IgG、IgM抗体均为阳性 ,总阳性率为 4 1.5 % ;留观人员IgG抗体阳性 1例 ;其他均为阴性。 结论 :ELISA测定血清抗SARS冠状病毒抗体阳性病例可确诊感染病毒 ,对于观察期后 (一般为 2 1d)人员 ,阴性结果可以排除感染。     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1.     

萌发花生种子中CaM含量变化与种子活力

应用ELISA测定了萌发花生种子中CaM的含量,种子的大部分CaM存在于胚轴中,小部分存在于子叶。高活力种子CaM含量较高,在吸胀48h出现一个特征峰,随种子活力下降,CaM含量也减少,其特征峰延迟至 72h才出现,且峰值也低,Ca~(++)可提高种子活力,也提高CaM的含量,使特征峰从72h提早到48h出现,TFP使种子CaM含量下降,直至萌发96h仍来山现特征峰,Ca~(++)、CaM与种子活力密切相关。