滁州市琅琊山明党参可溶性多糖分析

分别采用苯酚-硫酸法测定了采自滁州市琅琊山的明党参根及茎叶、药房购买的明党参和参照物党参中可溶性总糖含量、还原性多糖含量;利用薄层层析法定性分析了琅琊山明党参和药房购买的明党参多糖组成。结果表明,琅琊山明党参根中的可溶性总糖含量为4.07%,还原性多糖含量为4.58%。而药房购买的明党参中可溶性总糖含量达到了8.18%,还原性多糖含量为9.24%。利用优化后的展开剂正丁醇-异丙醇-乙酸-水(7:5:2:2)展层测定出琅琊山明党参根的样品中除了半乳糖外应该还含有分子量大于半乳糖的未知的糖,而购买的样品中除了麦芽糖和半乳糖外还含有分子量大于半乳糖的糖类。     

粉防己和木防己多糖的含量测定

目的 :测定中药粉防己和木防己中单糖和寡糖 (寡糖的量以单糖表示 )的总含量以及多糖的含量。方法 :单糖和寡糖的提取 :用 2 0倍溶媒 (6 0 %乙醇 )浸泡 2 4h后 ,于 5 0℃提取 4h ,共提取 3次 ,每次提取用 2 0倍溶媒。多糖的提取 :用 2 0倍溶媒 (水 )浸泡提尽单糖和寡糖的残渣 2 4h ,于 10 0℃提取 3h ,重复提取一次。检测方法为硫酸 -苯酚法。结果 :粉防己和木防己中单糖和寡糖 (寡糖的量以单糖表示 )的总含量分别为 10 .2 0 %和 7.75 % ;多糖的含量分别为36 .83%和 38.4 4%。结论 :粉防己和木防己中含有大量的多糖 ,为开辟防己类药材新的用途提供理论依据。     

槐角多糖及其水解物中单糖的检测

目的:测定槐角中多糖的含量及其多糖水解物中单糖。方法:利用分光光度法测定槐角多糖含量。利用三氟乙酸制备多糖水解物,采用毛细管电泳-安培检测槐角多糖水解物中单糖。结果:槐角中多糖的含量为3.12%。槐角多糖水解物中含有果糖和葡萄糖,两者的摩尔比为34.2∶1。结论:该方法准确、简便,可用于槐角中多糖及其多糖水解物中单糖的检测。     

女贞子及扶正女贞素片中多糖的含量测定

目的:建立女贞子及扶正女贞素片中多糖的含量测定方法.方法:采用苯酚-硫酸法测定总糖含量,采用水杨酸法测定单糖含量,从而准确测出多糖含量.结果:女贞子中多糖含量为2.712%,RSD=2.05%;扶正女贞素片中的多糖含量为3.952%,RSD=1.97%.结论:女贞子及扶正女贞素片中均含有大量多糖,该方法准确可靠,为扶正女贞素片的质量控制提供了依据.     

银耳菌丝体多糖理化性质及其吸湿保湿性能测定

目的:以银耳菌丝体为试验材料,制备银耳菌丝体多糖(PMT),研究其部分理化性质及吸湿保湿性能。方法:采用水提醇沉法制备PMT,硫酸-苯酚法测定其总糖含量,红外光谱法对其基团进行定性分析,ELSD-HPLC法测定其单糖组分,扫描电镜测定其表面形貌特征,称重法测定其吸湿与保湿性能。结果:PMT的总糖含量为81.52%,具有典型的多糖吸收峰,其单糖组成为Man∶Glc∶Gal=5.25∶2.82∶1.00,其表面不光滑,有许多呈花菜状凸起。在相对湿度43%、81%情况下,样品的吸湿率大小顺序为甘油PMT海藻酸钠。在干燥环境下,样品的保湿率大小顺序为PMT海藻酸钠甘油。结论:PMT是一种含有α-D-糖苷键的非淀粉类吡喃多糖,具有一定的吸湿保湿性能,可作为保湿因子在护肤品中具有开发应用潜力。     

反相-高效液体色谱法测定三种常见细菌胞外多聚物中的单糖组分

为研究细菌胞外多聚物(Extracellular polymeric substances,EPS)的复杂组分及其对环境中矿物细颗粒的溶蚀、改性作用,本文选取了土壤常驻菌硅酸盐细菌、空气常见致病菌金黄色葡萄球菌和人体常见菌大肠杆菌为研究对象,采用反相高效液相色谱(RP-HPLC)快速定性定量检测了三种细菌胞外多糖中单糖成分的方法 .结果表明:8种单糖在检测条件下进行了很好的分离,硅酸盐细菌胞外多糖中单糖成分有甘露糖和葡萄糖醛酸;金黄色葡萄球菌胞外多糖中单糖成分有核糖、葡萄糖,且在其生长过程中会消耗甘露糖、葡萄糖醛酸和半乳糖;大肠杆菌胞外多糖中单糖成分有核糖和半乳糖,且在其生长过程中会消耗甘露糖、葡萄糖醛酸、葡萄糖和半乳糖;不同微生物胞外多糖组成和含量上均有较大差异.该研究建立了用反相高效液相色谱快速测定细菌胞外多糖组分的方法 .     

鱼蓼中多糖和黄酮的含量分析

利用紫外分光光度法测定吸光度计算鱼蓼中多糖和总黄酮的含量.测得鱼蓼中总黄酮含量为4.257%,RSD=0.06%(n=5);多糖含量为5.889%,RSD=0.028%(n=5).建立了鱼蓼中多糖、总黄酮含量的测定方法,该方法简单、重现性好,可用于鱼蓼的质量控制和定量分析.     

北五味子中多糖含量的测定

目的:建立测定北五味子中多糖含量的方法.方法:采用比色法测定北五味子中多糖含量.结果:测得北五味子中多糖含量为6.57%,平均回收率为99.78%,RSD=1.54%.结论:该方法简便,精密度及重现性均较好.     

长型山药粘液质组分分析及抗肿瘤活性研究

本文测定了湖北利川长型山药的营养成分 ,并同时对鲜样、减压干燥和常压干燥三个处理的样品进行平行测定 ,研究了不同温度对山药营养成分的影响。从湖北利川山药中分离出粘液质 ,经组分测定为甘露聚糖一蛋白质复合物 ,经铜试剂沉淀 ,从粘液质中分离出水不溶性多糖A ;改用蛋白酶 -SEVAG法去蛋白质 ,DEAE -纤维素柱层析 ,分离得到水溶性多糖B。该粘液质溶于水 ,组分含量为总糖 2 1 .4%,氮 9.32 %,蛋白质 5 8.2 5 %。本文还研究了水溶性多糖的抗肿瘤活性 ,结果表明其对ES腹水癌有明显抑制作用。     

三叶木通果中主要营养成分含量的测定

分析测定了三叶木通果中VitaminC、粗蛋白质、还原糖和总糖含量 ,以及氨基酸组成和含量 .结果表明 :1 0 0g鲜果肉中VitaminC、粗蛋白质、还原糖和总糖的含量分别为 1 0 8mg ,1 .0 7g ,1 0 .2g和 1 4 .9g ;所测 1 7种氨基酸总量为81 8.5mg 1 0 0g ,其中含有 7种人体必需氨基酸为 2 98.4mg 1 0 0g ,占氨基酸总量的 36 .5 % .     

卡思卡特城堡

It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,     

不同的吃喝玩乐

一、吃和喝吃苹果 eat an apple, 吃药 take medicine,吃糖 have some sweets,吃饭 have one's meals,吃馆子 dine out,吃惊 be surprised/     

漏洞百出的教育

Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help?     

只要努力,不要后悔

There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love.     

浅谈移民问题

Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject.     

Sister Carrie, an Adherent of Desires

Sister Carrie is one of the most controversial characters in American literature.Thought as a "fallen woman" firstly,she was defined as a "new woman" by some critics later. However, by digging into the motivaton behind the whole process of Carrie's "success", the relationship between Carrie and her creator (the author), the social conditions of then American, it can be found that Carrie has never been free-standing on her thought and she has never found her real-sdf even after becoming a famous actress. In a society dominated by mass consumerism Carrie is only an adherent of her own desires. She also is a representative of all those country girls flooded into cities, a symbol and a sacrifice of the urbanization of America in a time countryside was overcome by cities.     

Improvement of one-cycle controller by use of proportional integral differential controller

1.IntroductionOne-cyclecontrolmethod,whichwasproposedaboutonedecadeago[1],hasbecomeanattractivemethodinspecialfieldssuchaspowerfactorcorrection[2-6],switchingamplifiers[7,8],etc.Themainideaofthiscontrollerisbasedonintegrationofdiodevoltageinone-cycleandforcesittobeexactlyequaltothereferencevalue.Themainadvantageofthiscontrollerisitsrealtimeabilitytorejectthevariationofinputvoltage[1].Despitethisgreatability,ithasnogoodperformancesinrejectingofloaddisturbanceandfollowingreferencecommands.Espec…     

库克斯顿城堡

城堡, 是欧洲典型的建筑之一, 每个城堡都有一个美丽的传说或是一段历史。从本期开始, 我们将推出“城堡之旅”, 带各位读者去浏览、观赏欧洲各地的城堡, 开始一段文化与历史的旅途!     

The Reader Response Approach to Teaching of English Literature

During interview with English majors, it was found that a majority of students expressed disappointment with their experience of English literature classes. Students' dislike of learning English literature appears to be the major problem of teaching English literature in China. Reader response approach is a good way to solve this problem. In this paper, I explain the rationale behind the reader response approach, such as its origin, definition, features, assumptions, and strategies. Then I illustrate how reader response approach works as a teaching strategy by presenting several teaching models. At last I evaluate its usefuiness for teachers and present the advantages of reader response approach in Chinese context.