蓖麻毒蛋白对泥鳅红细胞凋亡的诱导

分别用浓度0.02、0.04、0.06、0.08和0.10g/L的蓖麻毒蛋白对泥鳅进行急性毒性试验,在染毒处理第1、3、7、11d时将泥鳅断尾制血涂片,观察红细胞的凋亡情况.结果表明：蓖麻毒蛋白对泥鳅红细胞的凋亡具有明显的浓度和时间依赖性;染毒初期,泥鳅红细胞的凋亡率随蓖麻毒蛋白浓度的升高而不断增加;随染毒处理时间的延长,凋亡率曲线达峰值,随后凋亡率曲线不断降低,整个凋亡率曲线呈抛物线形.结论：蓖麻毒蛋白能诱导泥鳅红细胞凋亡.     

己烯雌酚对泥鳅红细胞的诱导作用

用不同浓度的己烯雌酚饲喂泥鳅,6d后取血制片,显微镜观察,以探讨不同浓度的己烯雌酚对泥鳅红细胞形成的影响。结果表明,己烯雌酚能诱导泥鳅红细胞产生异形细胞、异常核和微核;异形细胞率、微核率及异常核率与对照组比较,差异极显著(p<0.01),说明己烯雌酚对泥鳅红细胞的形成有明显的致突变作用。     

铬离子对泥鳅红细胞核异常的诱导

研究不同浓度及不同时间铬离子对泥鳅(Misgurnus anguilliaudatus)的血红细胞核异常的诱导作用。实验结果表明：铬离子对泥鳅红细胞核异常的诱导作用主要表现在引起核质外凸和核质内凹。多数试验组的核异常率高于对照组，显示铬离子对泥鳅红细胞的核异常具诱导作用。各试验组中泥鳅红细胞的核异常率呈一定的波动，在各时间段中随着时间的推移核异常率呈先升高后降低再升高的趋势；不同浓度的试验组诱导作用差异较大。     

垃圾渗滤液对泥鳅红细胞的诱变效应

通过不同浓度垃圾渗滤液对泥鳅红细胞微核、核异常及异形细胞影响的研究,以监测垃圾渗滤液中有害物质的诱变性。结果表明:垃圾渗滤液能诱导泥鳅红细胞产生微核,异常核和异形细胞。在处理浓度范围内,随着处理液浓度的增高,泥鳅红细胞微核率,核异常率和异形细胞率均升高,并且呈现一定的剂量效应关系。用蒸馏水作对照处理,经t检验,差异显著(p<0.05)或极显著,说明垃圾渗滤液对泥鳅红细胞有很强的致畸作用。     

铅胁迫诱导鸭血红细胞凋亡

用不同浓度的铅(Pb~(2 ))诱导鸭血红细胞凋亡,通过制作血涂片,Giemsa's染色,光镜下观察并记录细胞发生变化情况,测定凋亡率.结果表明,在P~(2 )的胁迫作用下,鸭血红细胞呈现细胞变圆、细胞膜突起形成泡状、核变圆、核边缘化、形成凋亡小体等凋亡形态特征,且低浓度的铅易导致细胞形态改变,高浓度的铅易引起细胞发生坏死.凋亡细胞随着Pb~(2 )浓度的增加和作用时间的延长而增加.     

乙酸铜对泥鳅红细胞微核的影响

以泥鳅为材料,研究不同浓度的乙酸铜对泥鳅外周血红细胞微核的影响。采用鱼类致突变实验方法,以0.15、0.45、1.5mg/kg不同剂量的乙酸铜处理泥鳅,测定泥鳅外周血红细胞微核率。结果发现,在乙酸铜浓度低于0.15mg/kg时,对红细胞的微核率影响不明显,当浓度大于0.45mg/kg时,红细胞的微核率与对照组差异显著。     

碳管法燃煤烟气汞浓度取样装置研制

自主研制了包括内置吸附剂和两段式碳吸附管在内的整套新型碳管法烟气汞浓度取样装置,以期实现燃煤烟气中颗粒汞和气相总汞浓度的精确测量．在6 kW 燃煤循环流化床装置上同时采用碳管法与安大略标准法（OHM）进行烟气中汞浓度取样．结果表明,碳管法所得汞平衡率均处于95．47％～104．72％之间．不同工况下碳吸附管第2段穿透率始终低于2％,且与相同工况下 OHM 测试结果的相对偏差在15．96％～17．56％之间,均小于20％．结果表明,所研制的碳吸附管干法烟气汞浓度取样装置符合美国 EPA 质量保证和质量控制（QA／QC）标准,可应用于实际燃煤烟气汞浓度的取样测试．     

敌敌畏诱发泥鳅红细胞微核及核异常的观察

本文研究了敌敌畏对泥鳅红细胞微核和核异常的诱发作用，结果表明；敌敌畏对泥鳅红细胞有明显的致突变作用，且微核率及核异常率与对照组比较差异极显著（t＜0．01）．     

细胞松弛素B和低温诱导华贵栉孔扇贝三倍体的产生(英文)

分别用细胞松驰素B（C．B）（浓度为0．5mg／L）和低温（10℃）处理诱导华贵栉孔扇贝Chlamysnobilis产生三倍体．抑制第一极体的三倍体诱导率达到44．1％和31．8％；而抑制第二极体的三倍体诱导率分别为90．2％和72．7％（胚胎初期检查）．随着C．B浓度的增加，孵化率下降；而随着温度的降低和休克时间的延长，非整倍体增多．     

敌敌畏诱发泥鳅红细胞微核及核异常的观察

本研究了敌敌畏对泥鳅红细胞微核和核异常的诱发作用，结果表明：敌敌畏对泥鳅红细胞有明显的致突变作用，且微核率及核异常率与对照组比较差异极显(t<0.01)。     

Oxidative stress and damage induced by abnormal free radical reactions and IgA nephropathy

Objective: To estimate the oxidative stress and oxidative damage induced by abnormal free radical reactions in IgA nephropathy (IgAN) patients' bodies. Methods: Seventy-two IgA N patients (IgANP) and 72 healthy adult volunteers (HAV) were enrolled in a random control study design, in which the levels of nitric oxide (NO) in plasma, lipoperoxide (LPO) in plasma and in erythrocytes, and vitamin C (VC), vitamin E (VE) and β-carotene (β-CAR) in plasma as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes were determined with spectrophotometric mothods. Results: Compared with the HAV group, the averages of NO in plasma, and LPO in plasma and in erythrocytes in the IgANP group were significantly increased (P＜0.0001), while those ofVC, VE and β-CAR in plasma as well as those of SOD, CAT and GPX in erythrocytes in the IgANP group were significantly decreased (P＜0.0001). Linear correlation analysis showed that with the increase of the values of NO, and LPO in plasma and in erythrocytes, and with the decrease of those ofVC, VE, β-CAR,SOD, CAT and GPX in the IgAN patients, the degree of histological damage of tubulointerstitial regions was increased gradually (P＜0.0001); and that with the prolongation of the duration of disease the values of NO, and LPO in plasma and erythrocytes were increased gradually, while those of VC, VE, β-CAR, SOD, CAT and GPX were decreased gradually (P＜0.005). The discriminatory correct rates of the above biochemical parameters reflecting oxidative damage of the IgAN patients were 73.8%-92.5%, and the correct rates for the HAV were 70.0%-91.3% when independent discriminant analysis was used; and the correct rate for the IgAN patients was increased to 98.8%, the correct rate for the HAV was increased to 100% when stepwise discriminant analysis was used. The above biochemical parameters' reliability coefficient (alpha) were used to estimate the oxidative damage of the IgAN patients as 0.8145, the standardized item alpha=0.9730, F=53273.5681, P＜0.0001. Conclusions: A series of free radical chain reactions caused serious pathological aggravation in the IgANP' bodies, thus resulting in oxidative damage in their bodies. In treating IgANP, therefore, it is necessary that suitable dose antioxidants should be supplemented to them so as to alleviate the oxidative damage in their bodies.     

鄱阳湖泥鳅染色体核型的研究

以鄱阳湖泥鳅头肾为材料，通过体内注射植物血凝素(PHA)经短期培养后，活体注射秋水仙素，空气干燥制片法制作染色体玻片标本，对泥鳅的染色体组型进行分析。结果表明：其二倍体染色体数目2n=50。核型公式有两种，分别是：8m 6sm 36t，NF=64；8m 6sm 2st 34t，NF=64。     

Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells

Objective: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. Methods: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. Results: NAT activity was lowered in apoptotic HepG2 cells;apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. Conclusion: NAT can inhibit apoptosis in HepG2 cells.     

H2S对兔左心室心肌细胞电生理特性的影响

目的:探讨硫化氢(H2S)对兔左心室心肌细胞电生理特性的影响.方法:用标准微电极细胞内记录技术,观测硫化氢对兔左心室心肌细胞电生理特性的影响.观测指标:静息电位(resting potential,RP)、超射值(over-shoot,OS)、动作电位幅值(amplitude of action po-tential,APA)、0相最大除极速率(maximal rate of depolarization,Vmax)、复极20%、50%和90%时间(20%、50%and 90% of duration of action potential,APD20、APD50and APD90)及复极化平均速度(average rate of repolarization).结果:(1)用50μmol/L NaHS 灌流时,和正常对照组相比,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).(2)100μmol/L NaHS与对照组比较,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).和50μmol/L NaHS相比APD50缩短(P＜0.01)、APD90缩短(P＜0.05)、复极化平均速度增快(P<0.05).(3)200μmol/L NaHS、400μmol/L、1 000μmol/L NaHS可浓度依赖性的使 APA、RP、OS减小(P<0.01),APD20、APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.01).结论:H2S可浓度依赖性的影响兔左心室心肌细胞电生理特性.     

柞蚕蛹中脂肪酸成分的GC/MS研究

采用索氏提取方法提取柞蚕蛹油,用KOH/CH3OH酯交换法甲酯化处理,以气相色谱/质谱方法测定脂肪酸的组成.共鉴定出13种脂肪酸成分,其中以亚麻酸(LNA)、棕榈酸(P)、硬脂酸为主,柞蚕蛹油中不饱和脂肪酸含量占总脂肪酸总量的66.63%.