5-脂氧合酶基因打靶小鼠及细胞核定位研究

简要介绍了5-脂氧合酶基因打靶小鼠模型的性质，在各种疾病模型 中的表型，以及5-脂氧合酶细胞核定位研究的新进展。     

胃癌复发研究现状与进展

淋巴结清扫范围是否充分及脱落癌细胞是影响胃癌治疗及患者预后的主要因素,淋巴结的清扫范围一直是存在争论的问题,同时脱落癌细胞的检测亦是治疗的主要方面,本文就上述两个问题的研究现状及进展作一综述。     

西藏地区16例藏族HER2阳性胃癌临床病理特征分析

目的 分研究西藏地区藏族HER2阳性胃癌患者的病理特征及临床资料分析,为本地胃癌患者规范化诊治提供参考。方法 选取2018年1月至2021年6月西藏自治区人民医院收治胃癌患者16例,收集以上患者的临床病理资料,全部病例均通过HER2免疫组化染色,判读HER2阳性状态,且对藏族胃癌HER2阳性胃癌的临床相关资料进行描述性分析。结果 182例患者接受HER2免疫组化检测。HER2阳性共16例,阳性率8.79%(16/182),其中男性11例,女性5例,年龄（34～76）岁。最常见的症状上腹饱胀感及伴疼痛14例14/16(87.5%),恶心7例7/16(44%),体重减轻5例5/16(31%)、黑便4例4/16(25%),饭后呕吐4例4/16(25%)。发病部位依次为：胃窦7例（43.7%）,全胃2例（12.5%）,贲门2例（12.5%）,胃体2例（12.5%）,胃角2例（12.5%）,胃底1例（6.2%）;肿瘤分期为：Ⅰ期0例,Ⅱ期2例（12.5%）,Ⅲ期3例（18.75%）,Ⅳ期11例（68.75%）。NRS 2002营养风险评分<3分,3/16(16%),≥3分13/16(84%...     

PCNA、C-erbB-2在胃癌中表达与生物学特性间意义的探讨

目的探讨PCNA、C -erbB - 2在胃癌中表达与生物学特性间的意义。方法用S -P免疫组化方法检验 72例胃癌中PCNA、C -erbB - 2表达情况。并就胃癌组织类型、浸润深度和淋巴结转移的关系应用Spss9.0版统计软件作X2 检验、Fisher精确检验及线性相关分析的统计学处理。结果PCNA、C-erbB - 2在胃癌不同分化程度的组织类型、浸润深度和有无淋巴结转移等生物学特性均无相关性 (P>0 .0 5 )。     

抽象环概念的历史演变

抽象环是抽象代数中的基本概念,重要性不言而喻。拟从抽象环概念的演变来解析它的来龙去脉,即如何从具体的数环、多项式环演变为现代数学中的抽象环。不仅能对抽象环的历史进行梳理考证,也对数学本身有一定的借鉴意义。     

赋值环的爆发

针对Hartshorne的Algebraic Geometry一书第4章习题12,通过爆发的无穷序列得到了离散和非离散2个赋值环,并具体给出了爆发的过程。     

3-甲叉-奎宁环碱氧化物的合成

以4-哌啶甲酸为原料,合成3-甲叉-奎宁环碱氧化物。考察反应温度、反应时间、反应物摩尔比、溶剂量等对产物的收率的影响。结果表明按此路线合成的3-甲叉-奎宁环碱氧化物成本较低、操作安全、质量均一稳定。     

学习与绩效:基于学习-绩效环的组织学习框架

在广泛的文献分析基础上,分析学习和绩效的关系,提出学习-绩效环的概念,给出一个基于学习-绩效环的组织学习框架:从组织目标和组织战略出发,包括三个层次—个体、团体、组织,和五个过程—解释、搜寻、实施、推广、反馈。并讨论该组织学习框架对知识管理和构建学习型组织的启示。     

浅析郑西基站环倒环失败故障处理

基站环倒环试验是GSM-R通信系统维护的年表项目之一,基站环不能正常进行环倒换,经过数次要点故障排查,最终定位了故障并加以解决.本论文阐述倒环试验步骤及倒环失败处理过程.     

化合物2,2-二甲基环戊酮合成工艺研究

以己二酸二甲酯为原料,在甲醇钠作用下关环,然后经过硫酸二甲酯甲基化作用,再经过脱脂反应得到中间体2-甲基环戊酮,最后在硫酸二甲酯甲基化作用下得到2,2-二甲基环戊酮。在2,2-二甲基环戊酮合成过程中考察了溶剂种类、反应温度、甲基化试剂种类及用量对甲基化作用的影响,得到以甲苯为溶剂、反应温度为35℃、硫酸二甲酯为甲基化试剂、2-甲基环戊酮与硫酸二甲酯比例为1∶1的最优甲基化工艺条件。     

Alteration of serum beta 2-microglobulin in oral carcinoma

Serum β₂-microglubin (β₂-m) levels were measured in oral carcinoma patients and compared with normal healthy controls. It was observed that there was a significant rise in serum β₂-microglobulin in oral carcinoma patients. Progressively higher values were obtained as the cancer advanced clinically. Therefore the estimation of serum β₂-microglobulin may be useful as one of a battery of tests in the assessment of oral carcinoma patients.     

Evaluation of immunohistochemistry and enzyme linked immunosorbent assay for HER-2/neu expression in breast carcinoma

HER-2 is overexpressed in approximately 20–30% of invasive Breast Cancer. ECD of the HER-2 protein is frequently cleaved and released into the circulation, where it can be detected by ELISA in up to 45% of patients with metastatic breast cancer. The objective of our study was to compare the current methods for the detection of HER-2 protein. Tissue HER-2 levels were studied in 100 breast cancer patients by IHC and compared with serum HER-2 levels by ELISA. IHC frequency was 29%. Serum HER-2 ECD was positive in 42% of patients. A statistically significant correlation was observed. HER-2 detected by IHC correlates significantly with serum HER-2 levels detected by ELISA. Thus, ELISA is a reliable and economical tool to assess the HER-2 status in tumors, when breast tissue sample is not available.     

基于B2B、B2C、C2C的第三方网上支付研究

研究第三方网上支付理论的基础上,对三种典型的电子商务B2B、B2C、C2C的第三方网上支付进行了比较分析,总结了基于B2B、B2C、C2C的第三方网上支付系统模式及其存在的问题,最后规划了第三方协同网上支付的构建模式及其特性。     

基于J2ME和J2EE的移动学习平台研究与设计

随着移动设备性能的不断提高,在移动设备上的应用也越来越广泛,移动学习正是随着移动信息技术发展而产生的一种新兴的学习方式。本文提出了基于J2ME和J2EE技术的移动学习平台的设计方案,这个平台采用J2ME技术作为客户端的实现技术以屏蔽移动设备间的巨大差异性,采用J2EE技术作为服务器端的实现技术以提供高效稳定的服务。     

利用H2O-NaCl-CaCl2体系水盐化合物和冰的融化温度计算NaCl/（NaCl＋CaCl2）比值和盐度的方程

The composition of fluid inclusions in the H2O-NaCl-CaCl2 system has been generally graphically estimated using the melting temperatures of hydrohalite （ Tm-HH ） and ice （ Tm-ice）. Here we present two equations that can be used to calculate the relative proportion of NaCl （ i. e. , NaCL/[ NaCl ＋ CaC12 ] , or XNacl ） and the total salinity （ i. e. , NaCl ＋ CaCl2, wt% ） for fluid inclusions with ice as the last melting phase. XNacl can be calculated from Tm-HH using the following equation： y = （a＋bx）^-1/c where y is XNacl, x is Tm_HH , a =0. 33124402, b =-0. 031518028, and c =0. 22932736. In the cases where only Tm-ice is measured and Tm-HH is not known, Tm-ice can be used as the maximum possible Tm-HH to calculate the maximum value of XNacl using the above equation. In these cases, the following equation can be used to calculate the maximum total salinity： y=（a＋bx＋cx2） l where y is salinity, x is Tm-HH , a =0, 057184817, b =0. 00078565757, and c = 5. 7262766E-6. Because the isothems in the field of ice are sub-parallel to the NaCl-CaCl2 binary side in the H2O-NaCl-CaCl2 ternary system, the errors in salinity calculation introduced by the above approximation are small （ less than 2 wt% ）. A Windows program for calculation of XNacl and salinity is available at ： http ：//uregina. ca/-chiguox.     

柠檬酸溶胶凝胶法制备铈锆固溶体的物性和氧化还原特性研究

以柠檬酸作为胶凝剂，采用溶胶凝胶法制备了一系列CeXZr1-XO2复合氧化物，分别经600℃和900℃焙烧，应用XRD检测，发现均已形成铈锆固溶体。并应用BET、XRD和TPR对其表相、晶相结构和氧化还原性质进行了分析，发现随着固溶体中铈比例的增加，固溶体稳定性降低；其储氧量测定显示铈锆固溶体用作三效催化剂助剂的最佳比例为1：1。经900℃高温焙烧以后，固溶体晶粒增大不明显，表明本方法适合制备高比表面积和高储氧量的铈锆固溶体；TPR还原峰温度降低，推测可能在于柠檬酸络合物完全分解所致。     

小议数字“两”、“二”和“2”的用法

列举若干实例，从习惯用法、具体含义和特殊用法讨论数字“两”、“二”和“2”的使用方法。     

基于J2EE的学习流系统设计与实现

学习流系统是运用工作流理论与技术而提出的一种面向学习过程的新型e-Learning学习平台.在介绍了学习流系统相关概念的基础上,提出了一种基于J2EE的学习流系统架构,并对各层进行了详细的设计,最后实现了一个学习流原型系统,较好地解决了目前e-Learning平台对学习过程支持不足的问题.     

Identification of Cyanobacteria-Based Natural Inhibitors Against SARS-CoV-2 Druggable Target ACE2 Using Molecular Docking Study,ADME and Toxicity Analysis

In 2019–2020, the novel “severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)” had emerged as the biggest challenge for humanity, causing “coronavirus disease 19 (COVID-19)”. Scientists around the world have been putting continuous efforts to unfold potential inhibitors of SARS-CoV-2. We have performed computational studies that help us to identify cyanobacterial photoprotective compounds as potential inhibitors against SARS-CoV-2 druggable target human angiotensin-converting enzyme (ACE2), which plays a vital role in the attachment and entry of the virus into the cell. Blocking the receptor-binding domain of ACE2 can prevent the access of the virus into the compartment. A molecular docking study was performed between photoprotective compounds mycosporine-like amino acids, scytonemins and ACE2 protein using AutoDock tools. Among sixteen molecularly docked metabolites, seven compounds were selected with binding energy < 6.8 kcal/mol. Afterwards, drug-likeness and toxicity of the top candidate were predicted using Swiss ADME and Pro Tox-II online servers. All top hits show desirable drug-likeness properties, but toxicity pattern analysis discloses the toxic effect of scytonemin and its derivatives, resulting in the elimination from the screening pipeline. Further molecular interaction study of the rest two ligands, mycosporine–glycine–valine and shinorine with ACE2 was performed using PyMol, Biovia Discovery studio and LigPlot+. Lastly biological activity of both the ligands was predicted by using the PASS online server. Combining the docking score and other studied properties, we believe that mycosporine–glycine–valine and shinorine have potential to be potent inhibitors of ACE2 and can be explored further to use against COVID-19.