计算机应用基础综合练习2

3　Ｗｉｎｄｏｗｓ上机题(1 )打开Ｗｉｎｄｏｗｓ 98资源管理器 ,完成以下操作 :ａ .在考生文件夹下的ＣＷＩＮＬＸ下创建一个名为ＡＢ1的文件夹 ;ｂ .将考生文件夹下的ＫＳ1 .ＴＸＴ及ＫＳ4 .ＴＸＴ文件复制到ＡＢ1文件夹中 ;ｃ.在考生文件夹下将ＫＳ3 .ＴＸＴ文件复制到ＬＳ1文件夹中 ,并换名为ＫＳ1 .ＤＯＣ ;ｄ .将ＫＳ5 .ＴＸＴ设置成“只读”属性 ;ｅ.删除考生文件夹下的ＬＳ文件夹 ;ｆ.将系统设置成“显示所有文件”后 ,去掉ＫＳ .ＴＸＴ文件“隐藏”属性 ;ｇ .利用查找功能查找ＳＯＷＥＲ .ＥＸＥ文件 ,并在ＡＢ1文件夹下建立ＳＯＷ…     

THE MODAL SYNTHESIS IN THE ANALYSISOF ROBOT KINETO-ELASTODYNAMICS

ＴＨＥＭＯＤＡＬＳＹＮＴＨＥＳＩＳＩＮＴＨＥＡＮＡＬＹＳＩＳＯＦＲＯＢＯＴＫＩＮＥＴＯ－ＥＬＡＳＴＯＤＹＮＡＭＩＣＳＪｉａｎｇＪｉａｎｈｏｎｓ（姜剑虹）（ＮａｎｊｉｎｇＥｎｇｉｎｅｅｒｉｎｇＩｎｓｔｉｔｕｔｅｏｆＥｎｇｉｎｅｅｒｉｎｇＣｏｒｐｓ）ＴＨ...     

THE TOTAL EFFBCT OF THE GREAT GATSBY

ＴＨＥＴＯＴＡＬＥＦＦＥＣＴＯＦＴＨＥＧＲＥＡＴＧＡＴＳＢＹＸｕＱｉｎ－ＳｕｎＴＨＥＧＲＥＡＴＧＡＴＳＢＹ，ｏｎｅｏｆＦｉｔｚｇｅｒａｌｄ’ｓｇｒｅａｔｅｓｔｎｏｖｅｌｓ，ｉｓａｎｏｄｄｃｏｍｂｉｎａ－ｔｉｏｎｏｆｒｏｍａｎｃｅａｎｄｒｅａｌｉｔｙ．...     

一类变分不等式解的存在性及应用

设Ｋ为Ｂａｎａｃｈ空间中的闭凸锥，本文讨论了〈Ｔ１ｘ－Ｔ２ｙ，ｙ－ｘ＞＋ｊ（ｙ）－ｊ（ｘ）≥０，ｙ∈Ｋ型变分不等式解的存在性，并应用所得结论讨论半线性椭圆方程解的存在性     

安全自由地改变硬盘分区

目前,常见的硬盘分区模式有：ＦＡＴ１６,ＦＡＴ３２,ＮＴＦＳ等,以前常用的ＦＡＴ１６这种分区模式,硬盘中簇的大小是根据分区的大小决定的,现在的大容量硬盘一般都是ＩＧＢ—２ＧＢ一个分区,每簇的大小是３２ＫＢ,如果存放一个只有ＩＫＢ的文件,那它在硬盘上实际占用３２ＫＢ,浪费了３１ＫＢ的空间,在小文件很多的情况下,浪费的空间就非常可观。ＦＡＴ３２分区的特点是每簇的大小都是４ＫＢ,支持大容量的分区。只可惜一般的ＤＯＳ和初始的ＷＩＮＤＯＷＳ９５以及一些防病毒软件,不能识别ＦＡＴ３２,这就存在一个如何自由改…     

不同配方的培养基对洋兰原球茎生长和增殖的影响

本试验以洋兰的原球茎（ＰｒｏｔｏｃｏｒｍＬｉｋｅＢｏｄｙ，简写成ＰＬＢ）为外植体，采用“ＭＳ＋１５％香蕉”为基本培养基，分别添加不同成份的激素（ＮＡＡ或ＫＴ或ＮＡＡ＋ＫＴ）和不添加任何激素，配制成四种不同配方的固体培养基进行培养，从而探索对兰花原球茎生长和增殖的影响。     

欧洲教师校本在职教育模式

欧洲教师校本在职教育模式北京师范大学教育系陈如平近些年来，在许多欧洲国家，教师的校本在职教育（Ｓｃｈｏｏｌ－ＢａｓｅｄＩｎｓｅｒｖｉｃｅＥｄｕｃａｔｉｏｎ）方兴未艾。根据教师在职教育研究小组研究者埃文斯（Ｔ．Ｋ．Ｅｖａｎｓ）的定义，所谓“校本在职教育...     

ANALYSIS OF THE FIELDS SCATTERED FROM A TWO－DIMENSIONAL CYLINDER ILLUMINATED BY A POINT SOURCE

ＡＮＡＬＹＳＩＳＯＦＴＨＥＦＩＥＬＤＳＳＣＡＴＴＥＲＥＤＦＲＯＭＡＴＷＯ－ＤＩＭＥＮＳＩＯＮＡＬＣＹＬＩＮＤＥＲＩＬＬＵＭＩＮＡＴＥＤＢＹＡＰＯＩＮＴＳＯＵＲＣＥＣｈｅｎｇＣｈｏｎｇｈｕ（程崇虎）ＺｈａｎｇＷｅｎｘｕｎ（章文勋）（ＳｔａｔｅＫｅｙＬａ...     

对96年一高考题的异议

对９６年一高考题的异议秦皇岛市第六中学（０６６０００）李俊生９６年高考化学试题Ａ卷第１１题：下列各组离子，在强碱性溶液中可以大量共存的是（ＡＫ＂Ｎａ＂ＨＳＯＳＣＩ－（ＢＮａ＋Ｂａ＇＂ＡＩＯ７ＮＯ６（Ｃ）ＮＨｔＫ＂ＣＩ－ＮＯＩ（ＤＫ＂Ｎａ＋ＣＩＯＳ＇＂...     

集成RS,JK,D触发器到T触发器的转换

本文了ＲＳ、ＪＫ、Ｄ触发器转换为Ｔ触发器的必要性，给出了ＲＳ、ＪＫ、Ｄ触发器到Ｔ良器的电路转换、特性方程转换，并由特性表和驱动表推出ＲＳ、ＪＫ、Ｄ触发器到Ｔ触发器的转换使用表。     

5-Fluorocytosine–Sugar Conjugates for Glucose Transporter-Mediated Tumor Targeting: Synthesis,Cytotoxicity, and Cellular Uptake Mechanism

Two novel sugar-conjugated 5-fluorocytosine (5-FC) antineoplastic compounds were designed and synthesized to improve the selective drug uptake by targeting the tumor-specific glucose transporter (GLUT).The antitumor activity of these compounds was evaluated in four different human cancer cell lines:A549 (human lung cancer cell line),HT29 (human colorectal cancer cell line),H460 (human lung cancer cell line),and PC3 (human prostate cancer cell line).The sugar conjugates exhibited cytotoxicity similar to or higher than 5-FC and 1-hexylcarbamoyl-5-FC in A549,HT29,H460,and PC3.Furthermore,GLUT-mediated transport of the glycoconjugate was investigated with GLUT inhibitor-mediated cytotoxicity analysis in a GLUT-overexpressing HT29 cell line.The cell-killing potency of 5-FC glycoconjugate was found to depend significantly on the GLUT inhibitor,and the cellular uptake of molecules was regulated by GLUT-mediated transport.All the results demonstrate the potential advantages of glycoconjugation for Warburg effect-targeted drug design.     

Study on the expression and mutation of human telomeric repeat binding factor ( hTRF1 ) in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108:_0.0749) than in normal mononuclear cells (0.0493, 0.0369:_0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease. Project supported by the National Basic Research Program (973) of China (No. 2002CB713700) and the National Natural Science Foundation of China (No. 39870339)     

开心散含药血清对皮质酮所致CTXTNA2细胞损伤的保护作用

目的：观察开心散含药血清对皮质酮（corticosterone）损伤大鼠大脑皮质星形胶质细胞CTXTNA2的影响。方法：制备开心散含药血清（500mg·kg-1）、阳性药氟西汀含药血清（10mg·kg-1）、普通大鼠血清。细胞实验中采用皮质酮损伤CTXTNA2细胞建立体外抑郁模型,MTT法检测开心散对皮质酮所致细胞损伤的影响以及磷脂酰肌醇3-激酶（phosphatidylinositol 3-kinase,P13K）信号通路抑制剂的干预作用;采用Western Blotting法检验相关信号通路蛋白表达变化情况。结果：皮质酮浓度为200μm01·L-1时,细胞活力抑制率可稳定在60％,浓度依赖性较好;开心散含药血清均能够显著提高皮质酮损伤细胞的存活率,而给予P13K相关通路的抑制剂后,开心散保护作用被逆转,Western Blotting结果显示给予开心散含药血清能够逆转皮质酮损伤后P13K及蛋白激酶B（protein kinase B,AKT）1／2蛋白表达下降的趋势。结论：开心散含药血清对皮质酮所致的CTXTNA2细胞损伤具有明显保护作用,其保护机制可能与P13K信号通路有关。     

Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation

Objective： To investigate the effect ofberbamine on human hepatoma cell line SMMC7721. Methods： The effects of 24 h and 48 h incubation with different concentrations （0-64 μg/ml） of the berbamine on SMMC7721 cells were evaluated using 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide （MTT） assay. Hoechst 33258 staining was conducted to distinguish the apoptotic cell, and the appearance of sub-G1 stage was determined by PI （propidium iodide） staining, the percentage of apoptotic cell was determined by flow cytometry following annexin V/PI staining. Flow cytometry was performed to analyze the cell cycle distribution and the mitochondrial membrane potential （△ψm）, the expression of activated caspase3 and caspase9 was analyzed by Western-blot. Results： The proliferation of SMMC7721 was decreased after treatment with berbamine in a dose- and time-dependent manner. Berbamine could induce apoptosis in SMMC7721 cells and could cause cell cycle arrest in G0/G1 phase, to induce loss of mitochondrial membrane potential （AVm） and activate caspase3 and caspase9. Berbamine-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk. Conclusion： Berbamine exerts antiproliferative effects on human hepatocellular carcinoma SMMC7721 cells. The anticancer activity of berbamine could be attributed partly to its inhibition of cell proliferation and induction of apoptosis in cancer cells through loss in mitochondrial transmembrane potential and caspase activation.     

Polyethylenimine-cyclodextrin-tegafur conjugate shows anti-cancer activity and a potential for gene delivery

Polyethylenimine-cyclodextrin-tegafur (PEI-CyD-tegafur) conjugate was synthesized as a novel multifunctional prodrug of tegafur for co-delivery of chemotherapeutic agent tegafur and enhanced green fluorescent protein (EGFP) reporter plasmid DNA. Conjugation of tegafur to PEI-CyD via chemical linkage was characterized by ¹H NMR spectrometry and ultraviolet (UV) spectrometry. PEI-CyD-tegafur was able to condense plasmid DNA into complexes of around 150 nm with positive charge at the N/P ratio of 25, in accordance with electron microscopy observation of compact and monodisperse nanoparticles. The results of in vitro experiments showed enhanced cytotoxicity and considerable transfection efficiency in B16F10 cell line. Therefore, PEI-CyD-tegafur may have great potential as a co-delivery system with anti-cancer activity and potential for gene delivery.     

莎士比亚的戏剧成就与“演讲”

莎士比亚在创作中为剧人物写下了大量演讲辞。演讲在莎士比亚戏剧中占有重要地位。演讲是莎氏戏剧思想的喷火口、情节的推动力,语言的锤炼场。     

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry. Western blot analysis was used to investigate the related mechanisms. Nude mice were further employed to investigate the antitumour activity of ART in vivo. MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose- and time-dependent manner. Based on the findings of light and transmission electron microscopy, Hoechst 33258 staining, and fluorescein isothiocyanate (FITC)-annexin V staining, the cytotoxicity of ART in HOS cells occurs through apoptosis. With ART treatment, cytosolic cytochrome c was increased, Bax expression was gradually upregulated, Bcl-2 expression was downregulated, and caspase-9 and caspase-3 were activated. Thus, the intrinsic apoptotic pathway may be involved in ART-induced apoptosis. Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G₂/M phase. In nude mice bearing HOS xenograft tumours, ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin, in agreement with in vitro observations. ART has a selective antitumour activity against human osteosarcoma HOS cells, which may be related to its effects on induction of apoptosis via the intrinsic pathway. The results suggest that ART is a promising candidate for the treatment of osteosarcoma.     

EGCG对肝癌细胞株BEL-7402增殖凋亡及Stat3蛋白表达的影响

研究了没食子儿茶素没食子酸酯（EGCG）对人肝癌细胞BEL-7402增殖凋亡及对信号转导蛋白和转录激活因子3（Stat3）蛋白及磷酸化Stat3蛋白表达的影响.应用MTT法检测不同浓度EGCG对BEL-7402细胞增殖的抑制作用,用流式细胞仪分析细胞的凋亡率,用ELISA方法检测EGCG对Stat3蛋白及磷酸化的Stat3蛋白表达的影响.结果表明：EGCG有抑制肝癌细胞增殖促进凋亡的作用,并呈剂量依赖性.ELISA结果显示EGCG能降低磷酸化Stat3蛋白的表达水平.EGCG能抑制肝癌细胞的增殖促进凋亡,其机制可能有与下调磷酸化的Stat3蛋白的表达有关.     

Parthenolide inhibits proliferation of vascular smooth muscle cells through induction of G0/G1 phase cell cycle arrest

Objective  This study is to determine the effect of the natural product parthenolide, a sesquiterpene lactone isolated from extracts of the herb Tanacetum parthenium, on the proliferation of vascular smooth muscle cells (VSMCs). Methods  Rat aortic VSMCs were isolated and cultured in vitro, and treated with different concentrations of parthenolide (10, 20 and 30 μmol/L). [³H]thymidine incorporation was used as an index of cell proliferation. Cell cycle progression and distribution were determined by flow cytometric analysis. Furthermore, the expression of several regulatory proteins relevant to VSMC proliferation including IκBα, cyclooxygenase-2 (Cox-2), p21, and p27 was examined to investigate the potential molecular mechanism. Results  Treatment with parthenolide significantly decreased the [³H]thymidine incorporation into DNA by 30%∼56% relative to control values in a dose-dependent manner (P<0.05). Addition of parthenolide also increased cell population at G₀/G₁ phase by 19.2%∼65.7% (P<0.05) and decreased cell population at S phase by 50.7%∼84.8% (P<0.05), which is consistent with its stimulatory effects on p21 and p27. In addition, parthenolide also increased IκBα expression and reduced Cox-2 expression in a time-dependent manner. Conclusion  Our results show that parthenolide significantly inhibits the VSMC proliferation by inducing G₀/G₁ cell cycle arrest. IκBα and Cox-2 are likely involved in such inhibitory effect of parthenolide on VSMC proliferation. These findings warrant further investigation on potential therapeutic implications of parthenolide on VSMC proliferation in vivo. Project (No. 491020-W50315) supported by the Foundation of the Health Bureau of Zhejiang, China     

上古汉语中的一些词义偏移现象

上古汉语“终”、“死”、“没”、“人”、“言”等词在特定的语境中词义向积极方面偏移。从这些词的词义特征和语境特征来看,偏移的词义是一种隐涵义,是语用推理的结果,没有成为词的固定义项。