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1.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献
2.
D. Vijayakumar K. Suresh S. Manoharan Ph.D. 《Indian journal of clinical biochemistry : IJCB》2006,21(1):105-105
The main objective of the study was to assess the oxidative stress in plasma and erythrocytes of rheumatoid arthritis patients
by measuring the levels of thiobarbituric acid reactive substances (TBARS), non-enzymatic antioxidants (vitamin E, C and reduced
glutathione) and enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx)]. This
study has been conducted on twenty-two adult female rheumatoid arthritis patients and an equal number of healthy subjects.
Elevated lipid peroxidation and multidirectional changes in the antioxidant defence system were noticed in patients with rheumatoid
arthritis. The enhanced lipid peroxidation accompanied by disturbance in antioxidant status indicates that rheumatoid arthritis
patients are more prone to free radical mediated oxidative damage. 相似文献
3.
Mridula Mahajan Sukhraj Kaur Shruti Mahajan Ravi Kant 《Indian journal of clinical biochemistry : IJCB》2009,24(2):205-207
Uric acid an endogenous aqueous antioxidant in normal humans is present in much higher concentrations than vitamin C and has
been known to cover 2/3rd of the free radical scavenging capacity in plasma. In the present study average uric acid levels of patients of rheumatoid
arthritis were found to be close to the normal individuals. A unique feature was observed after classifying the patients on
the basis of the duration of suffering, the patients having longer duration of disease had least uric acid levels as compared
to those suffering from relatively lesser period, similar trend was observed in the ascorbic acid estimations. The decline
in uric acid values with progression of the disease was much more than what was observed in case of ascorbic acid suggesting
the significant role of uric acid in scavenging of free radicals. Too much lowering of Uric Acid should be checked and vitamin
C should be supplemented in diet for maintaining the balance between pro oxidant and antioxidant forces to check pro oxidant
insult in rheumatoid arthritis. 相似文献
4.
BackgroundDiabetes is a metabolic disorder caused by defects in insulin production and activity. During disease progression, changes in lipid peroxidation cause structural modifications via production of free radicals. Fangchinoline is a well-known alkaloid present in Stephaniae tetrandrine S. Moore, which has demonstrated antioxidant, anticancer, and anti-inflammatory activities.ResultsThe present study analyzed the anti-diabetic and antioxidant effects of fangchinoline in male rats with streptozotocin-induced diabetes. Rats were divided into the following groups: normal control, diabetic, diabetic + fangchinoline 100 mg/kg, diabetic + fangchinoline 200 mg/kg and diabetic + glibenclamide 600 µg/kg. The treatment was administered orally for 45 consecutive days. Lipid peroxidation was substantially increased by >50% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. However, fangchinoline supplementation significantly reduced lipid peroxidation to near normal levels. Reactive oxygen species levels were substantially increased by >500% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. Fangchinoline supplementation reduced reactive oxygen species to near normal levels. Fangchinoline supplementation significantly improved superoxide dismutase, glutathione peroxidase, catalase, and reduced glutathione levels in diabetic rats. Total hexoses, sialic acid, hexosamines, and fucose were increased in diabetic rats, whereas fangchinoline supplementation significantly reduced these total hexoses, sialic acid, hexosamines, and fucose to near normal levelsConclusionsSupplementation with fangchinoline led to significant attenuation of the levels of lipid peroxidation, ROS, and glycoprotein components such as total hexoses, hexosamines, sialic acid, and fucose, while improving antioxidant marker levels.How to cite: Xia J, Huang W, Zhou F. Effect of fangchinoline on oxidant status in male albino rats with streptozotocin-induced diabetes. Electron J Biotechnol 2021;53. https://doi.org/10.1016/j.ejbt.2021.07.005 相似文献
5.
Abbas Ali Mahdi Anu Chandra Raj Kumar Singh Sanjeev Shukla L. C. Mishra Sohail Ahmad 《Indian journal of clinical biochemistry : IJCB》2003,18(2):8-15
In the present study the antioxidative potential ofMomordica charantia, Azadirachta indica, Allium sativum andOcimum sanctum was assessed in streptozotocin induced diabetic rats. Lipid peroxide levels were also measured in normal, diabetic and treated
animals. Malondialdehyde (MDA) levels were significantly higher and antioxidant activity was found low in diabetic groups
as compared to the control groups, and significant alteration in both the MDA levels and antioxidant activity was also observed
when the above herbal hypoglycemic agents were given to diabetic rats. On the basis of our results we conclude thatM. charantia, A. indica, A. sativum andO. sanctum are not only useful in controlling the lipid peroxide levels but are also helpful in further strengthening the antioxidant
potential. 相似文献
6.
Sarita N. Chavan Umesh More Shruti Mulgund Vishal Saxena Alka N. Sontakke 《Indian journal of clinical biochemistry : IJCB》2007,22(2):101-105
Osteoporosis encompasses a wide spectrum of conditions associated with imbalance of osteoclastic and osteoblastic activities.
The increased activity of osteoclasts leads to increased free radical formation and hence lipid peroxidation. Present study
probes into the role of antioxidants as a palliative treatment for osteoporosis. It involved 50 healthy controls and 75 clinically
diagnosed osteoporosis patients. Both the groups underwent baseline assessment of biochemical markers viz. osteoblastic markers:
serum Alkaline phosphatase. Free or ionic calcium and Inorganic phosphorus, osteoclastic markers: serum Tartarate resistant
acid phosphatase and Malondialdehyde and the antioxidant status: serum Superoxide dismutase and Erythrocyte reduced glutathione.
The osteoporotic group was then divided into groups A (Vitamin E-Evinal 400 mg), B (Vitamin C-Celin 500 mg), C (Vitamin E+C-Evinal+Celin)
for antioxidant supplementation for a period of 90 days. The results reveal that there is significant fall in concentration
of serum MDA (p<0.001), TrACP (p<0.01). Improvement in antioxidant status is reflected by significant rise in concentration
of serum SOD (p<0.001) and erythrocyte GSH (p<0.001) after 90 days of antioxidant supplementation in osteoporosis. The findings
indicate that on the whole bone status improved with prolonged antioxidant vitamin supplementation, which can be used as a
palliative treatment for osteoporosis. The efficacy is not affected whether the vitamins are administered singly or conjointly. 相似文献
7.
Madhur M. Goyal Devarshi U. Gajjar Dhara B. Patel Pradeep Sune Abhay R. Vasavda 《Indian journal of clinical biochemistry : IJCB》2009,24(4):375-380
Oxidative stress has been proposed as a common underlying mechanism of cataractogenesis. Experimental and observational data
suggest that micronutrients like vitamin C and vitamin E with antioxidant capabilities may retard the development of age-related
cataract. Effect of these factors on lens epithelium cells, center of lens metabolic activities, is not completely elucidated.
The aim of present study was to examine the effect of vitamin C and E on surgically removed lens epithelium cells of patients
with cataract. Capsulorhexis samples were collected from 170 patients, admitted for cataract surgery. Catalase specific activity
was estimated in lens epithelium cells with and without vitamin (C or E) treatment at different concentration for different
time duration. Student’s t-test was employed for data analysis. We observed that in ex-vivo condition, a) both vitamin C and
E bring about a decrease in catalase activity in lens epithelial cells. b) vitamin C showed toxic effect at high concentration.
c) 100μM was the optimum concentration at which both vitamins showed maximum antioxidant activity. It was concluded that both
vitamin C and E has direct effect on lens epithelium cells. At optimum concentration, they can reduce oxidative stress in
these cells thus can support to prevent or delay cataract development. 相似文献
8.
Harbans Lal Kiran Chugh Varinder Saini Jasbinder Kaur A. S. Saini 《Indian journal of clinical biochemistry : IJCB》1993,8(1):33-35
Glutathione-5-transferase (GST) and glutathione (GSH) levels were estimated in the lungs of 3 and 30 months old rats. GST activity was found to be significantly reduced in the old rats (30 months age) compared to the young rats (3 months age). In old rats, with methionine supplementation GST activity was increased whereas with vitamin C supplementation GSH levels were increased. The data show that the supplementation of methionine as well as of vitamin C have beneficial effect on the scavenging system and protect lungs against the free radical damage during ageing. 相似文献
9.
目的:研究四方藤60%乙醇提取物(EECH)对CII型胶原诱导类风湿关节炎模型(CIA)大鼠的抗风湿作用及其机制。方法:取40只Wistar大鼠随机分为正常对照组,模型组,阳性对照组,四方藤提取物高、低剂量组,每组8只。除正常对照组外,其余各组均采用CII胶原加氟氏完全佐剂法诱发类风湿性关节炎大鼠模型。EECH灌胃给予不同剂量提取物(2、0.5g.kg-1.d-1),模型组(雷公藤多苷片,1.5mg.kg-1.d-1),其余两组给予等量生理盐水,连续28天。测定大鼠血清炎症因子中TNF-α、IL-1β的表达,观察各组大鼠关节组织病理变化情况。结果:与模型组比较,EECH高、低剂量组可以显著降低CIA大鼠血清中炎症因子TNF-α、IL-1β的表达(P0.01)。病理检测表明,EECH可以显著改善CIA大鼠的关节病变。结论:EECH可对类风湿关节炎模型大鼠的关节具有明显的治疗作用,其机制可能与其下调血清中炎症因子中TNF-α、IL-1β的表达有关。 相似文献
10.
Nalini Ganesan Hariprasad Chegu A. N. Chandrasekaran 《Indian journal of clinical biochemistry : IJCB》2003,18(2):216-222
Adjuvant induced arthritis (AIA) is a model widely used to study Rheumatoid arthritis (RA). In the present study, lipid peroxides
level in spleen and thymus of AIA rats was observed to be significantly high compared to normal rats. A significant decrease
in ascorbic acid (ASA), reduced glutathione (GSH), superoxide dismutase activity (SOD) was also observed in spleen and thymus
of AIA rats compared to normal rats. There was also a steady increase in the circulating immune complex level (CIC) throughout
the experimental period in serum of AIA rats. In the present investigation, it was decided to study the effect of pre and
post treatment with TYPE II collagen on the antioxidant status and the circulating immune complex level in AIA rats. The results
from the present work indicates that the pretreatment with TYPE II collagen was effective in bringing significant changes
on all the parameters studied in AIA rats. The post treatment with TYPE II collagen was effective in bringing significant
changes on the CIC immune complex level and GSH content in the thymus tissue of AIA rats. The present work suggests that the
pre treatment with TYPE II collagen was more effective in suppressing the disease than the post treatment. 相似文献
11.
C. S. Parameswari B. Vijayageetha R. Vijayakumar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):131-136
The oxidative modification of lipoprotein especially low density lipoprotein (LDL) plays a key role in the initiation and
progression of atherosclerosis. Serum apolipoproteinB (apoB) level is found to be an important marker for atherosclerosis.
The present paper focuses on the measurement of serum apoB levels and the effect of Vitamin E, Vitamin C and reduced glutathione
on the copper ion induced oxidation of LDL + VLDL (VLDL-Very Low Density Lipoprotein) lipoprotein fraction isolated from the
serum of chronic renal failure (CRF) and renal transplanted patients. The level of apoB is significantly higher in the serum
of transplanted patients when compared to CRF patients and normal subjects. The level of TBARS formed in oxidized LDL + VLDL
lipoprotein fraction of CRF and renal transplanted patients are significantly increased than normal even in the presence of
antioxidants but the level of TBARS produced, with antioxidants is comparatively lesser than that produced by oxidized lipoprotein
fraction without antioxidants. It may be concluded than that produced by oxidized lipoprotein fraction without antioxidants.
It may be concluded that oxidation of LDL can be prevented at an earlier stage by Vitamin E supplementation. The supplementation
with Glutathione serves as the best method of preventing the lipoprotein oxidation among the renal diseased patients. 相似文献
12.
绿色智造是传统制造业转型发展的趋势.以湖州绿色智造产业园为例,探究先进制造业集群发展.从发展规划、集群创新性、开放性、集群效应、产业链布局等方面探讨存在的问题.提出先进制造业集群发展对策建议.加强整体规划,做好集群顶层设计;完善体系建设,构建集群创新体系;夯实技术基础,推进集群技术创新;提高开放水平,扩大集群开放合作;... 相似文献
13.
J. O. Olanlokun 《Indian journal of clinical biochemistry : IJCB》2008,23(1):62-66
The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels,
antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated
lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that
oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the
diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while
the level of lipid peroxidation decreased. 相似文献
14.
Ayfer Colak Burak Toprak Nese Dogan Fusun Ustuner 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2013,23(3):321-325
Introduction:
Studies about vitamin D [25(OH)D] stability in plasma are limited and preanalytical variables such as tube type may affect results. We aimed to evaluate effect of storage conditions, sample type and some preanalytical variables on vitamin D concentration.Materials and methods:
Blood samples from 15 healthy subjects were centrifuged at different temperatures and stored under different conditions. Serum and plasma 25(OH)D difference, effect of centrifugation temperature and common storage conditions were investigated.Results:
There was no difference between serum and plasma vitamin D concentration. Centrifugation temperature had no impact on vitamin D concentration. 25(OH)D is stable under common storage conditions: 4 hours at room temperature, 24 hours at 2–8 °C, 7 days at −20 °C, 3 months at −80 °C.Conclusion:
Vitamin D does not require any special storage conditions and refrigeration. Both serum and plasma can be used for measurement. 相似文献15.
随着人们对绿色消费模式的追求,加速了企业绿色转型发展,企业绿色度评价问题逐渐引起学术界的关注。本文从企业绿色技术、企业绿色生产、企业绿色排放、企业绿色投入、绿色企业文化5个方面选取了企业绿色度评价指标体系,并运用AHP与标准离差相结合的方法来确定指标体系的组合权重,依据可拓学原理构建企业绿色度可拓学评价模型。结合云南某生物科技股份有限公司2008年、2012年的发展情况进行了实证评价。结果显示该企业从2008年到2012年,大部分绿色度指标都有了较大提升,均提升到了1级水平,表明企业越来越重视绿色化发展模式,总体上取得了较好的效果,并具体分析了企业绿色度变化情况,为公司未来绿色发展提供决策指导,也为我国企业绿色度评价提供理论参考。 相似文献
16.
Anissa Kaleemullah Shariff Sangita R. Patil Pramod S. Shukla Ajit V. Sontakke Anup S. Hendre Anand K. Gudur 《Indian journal of clinical biochemistry : IJCB》2009,24(3):307-311
The objectives of the present study were to ascertain the variations in the serum levels of malondialdehyde and total antioxidant
status, in head and neck malignancies with different stages, with and without oral antioxidant supplementation, before and
after radiotherapy, and to validate the protective effects of an antioxidant supplementation during radiotherapy. The pretreatment
values of serum malondialdehyde were significantly raised, while that of serum total antioxidant status were significantly
declined in all the stages of head and neck malignancies, when compared with the healthy controls values (P<0.001). A significant
correlation was observed related to the studied parameters and different stages of the disease. The study suggests that an
oral antioxidant supplementation during radiotherapy is an effective mode in reducing oxidative stress. Antioxidant supplementation
during radiotherapy may serve as an adjuvant therapy in malignancies offering a protection to normal cells that may further
reduce the risk of developing secondary cancers. 相似文献
17.
The objective of the present study is to evaluate the beneficial effect of tomatoes, which are rich source of Lycopene, relatively
a new carotenoid known to play an important role in human health and disease. In this study lipid peroxidation rate was measured
by estimating Malondialdehyde and the levels of serum enzymes involved in antioxidant activities like Super Oxide Dismutase,
Glutathione Peroxidase, Glutathione Reductase, Reduced Glutathione, in type-II diabetic group (n=40) and age matched control
group (n=50), and observed significantly lower levels of antioxidant enzymes and very high lipid peroxidation rate in type-II
diabetes when compared to control group (p<0.001). Short term supplementation with tomatoes (cooked) to diabetic group for
a period of 30 days, showed a significant improvement in antioxidant enzyme levels (p<0.001) and decreased lipid peroxidation
rate (p<0.001) suggesting the supplementation with tomato lycopene may serve as the best method of preventing the oxidative
stress in diabetic patients. 相似文献
18.
19.
Rashmi Raghuvanshi M. Chandra P. C. Misra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2005,20(1):26-29
Platelets play important role in precipitating ischaemic myocardial syndromes in many ways. One of the consequences of ischaemic
diseases is excessive generation of oxygen derived free radicals that have numerous pathophysiological consequences. Platelet
pro-oxidant enzyme, xanthine oxidase is one of the sources of generation of free radicals. In the present paper, we report
the effect of administration of vitamin E along with aspirin on the levels of platelet xanthine oxidase and extent of free
radical mediated damage in the patients reperfused after myocardial infarction.
Our findings show that administration of 400 mg. vitamin E for six days along with 80 mg. aspirin has an excellent anti-oxidant
effect as evidenced by reduced platelet xanthine oxidase activity and lowering of malondialdehdye (MDA) levels which is an
index of the extent of free radical mediated damage. 相似文献