包涵体蛋白的变复性研究

针对基因工程蛋白在E.coli中表达多为无活性包涵体的特点，介绍了包涵体洗涤和去折叠的方法，蛋白质体外折叠的过程和机理，体外复性技术以及复性蛋白的检测，着重讨论了最近发展起来的蛋白质复性新技术。     

重组人粒细胞刺激因子蛋白特性及蛋白重折叠过程杂质研究

重组人粒细胞刺激因子(rhG-CSF)是用于治疗化疗所致的中性粒细胞减少症。来源于基因工程的细胞表达的包涵体,包涵体经过变性、复性和分离纯化等步骤处理后得到药用级别的rhG-CSF。本文根据重组人粒细胞刺激因子蛋白结构特性,对包涵体裂解复性过程中蛋白质重折叠过程进行总结,通过对其重折叠过程的研究,以便于找寻到更优化裂解复性条件,提高下游生产回收率,提高工业生产效益。     

含超临界CO_2体系的相平衡计算

一、引言用超临界流体作为萃取剂来分离、提纯化合物就是新近发展的超临界流体萃取技术。它不仅能在化工领域中应用,而且已为石油、食品、煤炭、医药、烟草等工业所采用。国外的研究非常活跃,国内也已起步。该技术开发的一个重点就是其基础理论的研究,包括相平衡的计算,分离效率的确定等。超临界流体萃取与其它萃取过程一样,属于平衡分离过程,因而在论证其方案,确定工艺指标和设备设计中,都需要有此过程中涉及的相平衡数据。我们针对超临界流体萃取技术用于醇——水体系分离和用超临界CO_2回收石油过程中涉及的部分相平衡关系,用状态方程法进行关联计算,作为研究超临界流体萃取技术的出发点。选用十个含超临界CO_2的二元系进行计算,考察了有关状态方程的适用性。     

浅谈超临界CO2萃取技术及其在中药研究中的应用

超临界CO2萃取技术作为一种新型绿色的分离手段在中药中的应用日益广泛。本文简述了超临界CO2萃取技术的原理、流程、在中药提取分离中的应用及其优缺点,并探讨了影响中药超临界CO2萃取的因素。     

稀土溶剂萃取的新进展

本文综述了作者近年来在国家自然科学基金资助下,在稀土溶剂萃取领域的研究中所取得的一些新进展。主要内容有:(1)串级萃取理论及其在稀土最优化分离工艺设计中的应用,(2)多组分稀土萃取分离中的三出口新工艺的设计和应用,(3)稀土萃取分离工艺的自控软件和专家系统的研究,(4)各类萃取体系的萃合物结构和萃取机理的系统研究。     

山茶油的超临界CO萃取工艺分析

初步研究应用超临界CO2萃取山茶油的工艺，探索在不同投料量、萃取压力、萃取温度、萃取时间、分离温度等对超临界CO2萃取的影响。结果表明，萃取压力35～40MPa，萃取温度30℃；分离釜Ⅰ压力8～11MPa，温度55～75℃；分离釜Ⅱ温度室温～40℃，即可获得比较好的萃取率，萃取的山茶油纯度较高；在成型的萃取设备中，小萃取釜的工艺参数对大釜参数的选择有着较大的参考作用。     

以环己醇为溶剂连续萃取精馏分离丙酮-正己烷

本文应用化工过程模拟软件Aspen Plus V7.3对丙酮—正己烷最低共沸物系的连续萃取精馏过程进行了模拟与优化。通过绘制拟二元汽液平衡相图,筛选出合适的萃取剂为环己醇,确定了双塔连续萃取精馏的工艺流程,并利用灵敏度分析工具考察了萃取精馏塔的全塔理论板数、原料进料位置、萃取剂进料位置、回流比、溶剂比(萃取剂对原料的摩尔比)对分离效果的影响。确定的最佳工艺方案为:全塔理论板数为33,原料和萃取剂分别在第29块和第3块理论板进料,回流比为0.3,溶剂比为3。在此工艺条件下:萃取精馏塔塔顶丙酮的分离效果达99.98%,萃取剂再生塔顶正己烷的纯度达到99.89%。模拟与优化结果为丙酮—正己烷共沸物连续萃取精馏分离过程的设计和操作提供了参考。     

制药分离技术探析

在制药工程中,制药分离技术是不可缺少的重要的一个环节。其中比较常用的分离技术包括固液萃取、超临界流体萃取、反胶团萃取、双水相萃取和沉析等。掌握和选择这些分离技术,就可以适应大规模工业生产的需要。     

以二甲基亚砜为溶剂连续萃取精馏分离二异丙醚-异丙醇

本文应用化工过程模拟软件Aspen Plus V7.3对二异丙醚-异丙醇最低共沸物系的连续萃取精馏过程进行了模拟与优化。通过绘制拟二元汽液平衡相图,筛选出合适的萃取剂为二甲基亚砜(DMSO),确定了双塔连续萃取精馏的工艺流程,并利用灵敏度分析工具考察了萃取精馏塔的全塔理论板数、原料进料位置、萃取剂进料位置、回流比、溶剂比(萃取剂对原料的摩尔比)对分离效果的影响。确定的最佳工艺方案为:全塔理论板数为20,原料和萃取剂分别在第15块和第5块理论板进料,回流比为0.5(mole),溶剂比为0.25(mass)。在此工艺条件下:萃取精馏塔塔顶二异丙醚的分离效果达99.86%,萃取剂再生塔塔顶异丙醇的纯度达到95.31%。模拟与优化结果为二异丙醚-异丙醇共沸物连续萃取精馏分离过程的设计和操作提供了参考。     

萃取精馏分离甲醇和丙酮共沸物

采用萃取精馏对甲醇和丙酮体系进行分离模拟和试验。采用HYSYS2．2软件对以水作溶剂分离甲醇和丙酮混合物的萃取精馏过程进行了模拟，得出了最佳工艺奈件；在模拟的最佳工艺条件下，对以水为溶剂萃取精馏分离甲醇和丙酮混合物进行了试验研究，试验结果和模拟计算相吻合，从而验证了模拟的可靠性。     

促蛋白复性高分子及其作用机制

概述近年来蛋白复性中所使用的高分子添加剂及各自的作用机制,为相关领域的研究者对促蛋白用高分子的选择以及新型材料的研制,提供一定的研究基础和理论依据.     

桂西铝土矿选矿工艺流程优化实践

桂西铝土矿属于岩溶堆积型铝土矿,矿产资源十分丰富,远景储量高达5.18亿吨。然而,在开发过程中发现,采场难洗胶泥矿储量大,矿石与泥团难分离,原设计的选矿工艺流程无法满足胶泥矿的矿石与泥团分离过程,处理胶泥矿时,产量受到极大的影响。针对此问题,广西华银铝业有限公司在工艺流程上作了改进,试图解决胶泥矿难处理、处理量低的问题。     

某铜锌矿选矿小型试验研究

根据某铜锌矿矿石性质特性进行小型试验,初步探索处理该矿石的选矿工艺:采用为浮重原则流程,浮选回收铜、锌,获得最终铜精矿、锌精矿,浮选尾矿进行硫砷分离,获得硫精矿和最终砷精矿。铜锌浮选分离为流程的关键作业,试验进行了3种浮选方案比较,捕收剂以Z-200效果较好,工艺以优先有氰浮选结果较优。     

Rapid separation of bacteriorhodopsin using a laminar-flow extraction system in a microfluidic device

A protein separation technology using the microfluidic device was developed for the more rapid and effective analysis of target protein. This microfluidic separation system was carried out using the aqueous two-phase system (ATPS) and the ionic liquid two-phase system (ILTPS) for purification method of the protein sample, and the three-flow desalting system was used for the removal of salts from the sucrose-rich sample. Partitioning of the protein sample was observed in ATPS or ILTPS with the various pHs. The microdialysis system was applied to remove small molecules, such as sucrose and salts in the microfluidic channel with the different flow rates of buffer phase. A complex purification method, which combines microdialysis and ATPS or ILTPS, was carried out for the effective purification of bacteriorhodopsin (BR) from the purple membrane of Halobacterium salinarium, which was then analyzed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and matrix-assisted laser desorption∕ionization time-of-flight. Furthermore, we were able to make a stable three-phase flow controlling the flow rate in the microfluidic channel. Our complex purification methods were successful in purifying and recovering the BR to its required value.     

Deformability-based circulating tumor cell separation with conical-shaped microfilters: Concept,optimization, and design criteria

Circulating tumor cells (CTCs) separation technology has made positive impacts on cancer science in many aspects. The ability of detecting and separating CTCs can play a key role in early cancer detection and treatment. In recent years, there has been growing interest in using deformability-based CTC separation microfilters due to their simplicity and low cost. Most of the previous studies in this area are mainly based on experimental work. Although experimental research provides useful insights in designing CTC separation devices, there is still a lack of design guidelines based on fundamental understandings of the cell separation process in the filters. While experimental efforts face challenges, especially microfabrication difficulties, we adopt numerical simulation here to study conical-shaped microfilters using deformability difference between CTCs and blood cells for the separation process. We use the liquid drop model for modeling a CTC passing through such microfilters. The accuracy of the model in predicting the pressure signature of the system is validated by comparing it with previous experiments. Pressure-deformability analysis of the cell going through the channel is then carried out in detail in order to better understand how a CTC behaves throughout the filtration process. Different system design criteria such as system throughput and unclogging of the system are discussed. Specifically, pressure behavior under different system throughput is analyzed. Regarding the unclogging issue, we define pressure ratio as a key parameter representing the ability to overcome clogging in such CTC separation devices and investigate the effect of conical angle on the optimum pressure ratio. Finally, the effect of unclogging applied pressure on the system performance is examined. Our study provides detailed understandings of the cell separation process and its characteristics, which can be used for developing more efficient CTC separation devices.     

肾叶细辛总黄酮的分离纯化工艺研究

本研究通过对大孔吸附树脂的简单介绍,讨论了几种常见化合物的研究进展。以动态和静态吸附率及解析率为指标,通过考察AB-8、LAS-10、D101和HPD-100四种大孔树脂对肾叶细辛总黄酮的吸附和解吸性能,从中筛选适用的大孔树脂类型,以期为深入挖掘肾叶细辛黄酮的分离纯化工艺提供新思路。     

模块化创新企业间的价值吸收——以丰田汽车公司为例的分析

 随着模块化研究的深入,主流文献逐渐将视角聚焦于技术创新的模块化,模块化创新开始成为学界研究的核心问题。基于吸收能力与企业间网络理论,从模块化影响企业创新的路径与机理角度,探讨了模块化创新企业间的价值吸收问题。首先,分析了模块化创新企业间价值吸收的本质,即产品内分工深化引起的企业能力生成和价值生成的分离,具体表现为：（1）能力生成边界和能力作用边界的分离,（2）价值生成和价值整合的分离。其次,探讨了模块化创新企业间的价值吸收机制,指出价值吸收包括共享吸收、互补吸收、单体吸收等三条路径,需经历搜寻、甄选、内化、整合、实现等五阶段过程。最后,通过对丰田公司的案例分析,论证了文中提出的理论观点,也进一步拓展了模块化创新的实践范畴。     

Surface protein gradients generated in sealed microchannels using spatially varying helium microplasma

Spatially varied surface treatment of a fluorescently labeled Bovine Serum Albumin (BSA) protein, on the walls of a closed (sealed) microchannel is achieved via a well-defined gradient in plasma intensity. The microchips comprised a microchannel positioned in-between two microelectrodes (embedded in the chip) with a variable electrode separation along the length of the channel. The channel and electrodes were 50 μm and 100 μm wide, respectively, 50 μm deep, and adjacent to the channel for a length of 18 mm. The electrode separation distance was varied linearly from 50 μm at one end of the channel to a maximum distance of 150, 300, 500, or 1000 μm to generate a gradient in helium plasma intensity. Plasma ignition was achieved at a helium flow rate of 2.5 ml/min, 8.5 kV_pk-pk, and 10 kHz. It is shown that the plasma intensity decreases with increasing electrode separation and is directly related to the residual amount of BSA left after the treatment. The plasma intensity and surface protein gradient, for the different electrode gradients studied, collapse onto master curves when plotted against electrode separation. This precise spatial control is expected to enable the surface protein gradient to be tuned for a range of applications, including high-throughput screening and cell-biomolecule-biomaterial interactions.     

Size and shape based chromosome separation in the inertial focusing device

In this paper, we use a spiral channel inertial focusing device for isolation and purification of chromosomes, which are highly asymmetric. The method developed is proposed as a sample preparation process for transchromosomic research. The proposed microfluidics-based chromosome separation approach enables rapid, label-free isolation of bioactive chromosomes and is compatible with chromosome buffer. As part of this work, particle force analysis during the separation process is performed utilizing mathematic models to estimate the expected behavior of chromosomes in the channel and the model validated with experiments employing fluorescent beads. The chromosome sample is further divided into subtypes utilizing fluorescent activated cell sorting , including small condensed chromosomes, single chromosomes, and groups of two chromosomes (four sister chromatids). The separation of chromosome subtypes is realized based on their shape differences in the spiral channel device under high flow rate conditions. When chromosomes become aligned in the shear flow, the balance between the inertial focusing force and the Dean flow drag force is determined by the chromosome projection area and aspect ratio, or shape difference, leading to different focusing locations in the channel. The achieved results indicate a new separation regime in inertial microfluidics that can be used for the separation of non-spherical particles based on particle aspect ratios, which could potentially be applied in fields such as bacteria subtype separation and chromosome karyotyping.     

广西某低品位难选氧化锰矿选矿试验研究

根据广西某低品位氧化锰矿石原矿性质特点,试验采用两矿法酸浸及强磁选方案比较。酸浸试验显示锰浸出率高达94.89%;强磁选获得的试验指标：锰品位22.11%、MnO2品位28.01%、锰回收率70.40%。