共查询到20条相似文献,搜索用时 593 毫秒
1.
Anjali C. Warrier Narasimha Y. Rao Devdutta S. Kulpati Tarun K. Mishra Bhaskar C. Kabi 《Indian journal of clinical biochemistry : IJCB》1995,10(1):9-13
There was increase in erythrocyte lipid peroxidation and adenosine deaminase (ADA) activity in red blood cells (RBC's), white
blood cells (WBC's) and plasma, in 30 patients of confirmed diabetes mellitus, 10 each of insulin dependent diabetes mellitus
(IDDM or type I), non-insulin dependent diabetes mellitus (NIDDM or type II) and diabetes with ketoacidosis when compared
with 20 healthy individuals (controls). Glycosylated hemoglobin A1c%, plasma free fatty acid (FFA) and glycerol levels were
also elevated in all the cases when compared with controls. The increase was more in diabetic ketoacidosis group. Significant
positive correlations were seen between erythrocyte lipid peroxidation and ADA activities in RBC's, WBC's and plasma. It is
suggested that decreased tissue adenosine levels due to increase in ADA activity, is related to the severity of hyperglycemia
and lipid peroxidation in diabetes mellitus. 相似文献
2.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献
3.
D. Vijayakumar K. Suresh S. Manoharan 《Indian journal of clinical biochemistry : IJCB》2005,20(1):52-55
The present study has investigated the levels of lipids, lipoprotein cholesterol (HDL and LDL cholesterol), thiobarbituric
acid reactive substances (TBARS) and vitamin E in plasma and erythrocyte membranes of twenty two clinically diagnosed adult
rheumatoid arthritis patients and an equal number of age matched healthy subjects. The levels of lipids and lipoprotein cholesterol
were markedly reduced in patients with rheumatoid arthritis as compared to healthy subjects. The altered lipid pattern may
be related to decreased lipoprotein cholesterol, fatty acids and impairment in antioxidant defence mechanism. 相似文献
4.
Gauri S. Vahalkar Vijaya A. Haldankar 《Indian journal of clinical biochemistry : IJCB》2008,23(3):223-226
Glyco-oxidation is considered as a source of permanent, cumulative, oxidative damage to long lived proteins in ageing and
in diabetes. Although RBC depends solely on glucose for energy purpose, hyperglycemic state glycosylates hemoglobin, creates
oxidative stress and puts the cellular components at risk. In view of this, RBC membrane composition was analyzed in diabetic
patients. The results were compared with healthy age and sex matched control groups. When RBC membrane components such as
protein, sialic acid, phospholipids and cholesterol were determined in insulin dependent diabetes mellitus, a significant
rise in phospholipids and cholesterol and significant fall in sialic acid and protein content was noted. RBC membrane composition
showed pronounced alterations in insulin dependent diabetes mellitus. These changes were accompanied by higher levels of lipid
peroxidation products like Malondialdehyde. 相似文献
5.
Ashavaid T. F. Shalia K. K. Nair K. G. Dalal J. J. 《Indian journal of clinical biochemistry : IJCB》2000,15(1):1-5
The levels of cytotoxic aldehydic products in different culinary oils, with or without thermal stress, (routine domestic or
commercial frying) were determined by thiobarbituric acid method. The results showed that (i) thiobarbituric acid reactivity
was much higher in edible oils rich in polyunsaturated fatty acids than those rich in saturated fatty acids or monounsaturated
fatty acids, even without thermal stress, (ii) the lipid peroxide levels were in proportion to the duration of thermal stress,
(iii) nature of the container used (steel, iron or teflon-coated) had no significant effect on the extent of lipid peroxidation
under identical conditions of thermal stress and (iv) thermally stressed oils collected from hotels and roadside caterers
contained higher levels of cytotoxic aldehydic products, when compared to oils thermally stressed under domestic frying conditions.
These results suggest that dietary ingestion of thermally or autoxidatively stressed polyunsaturated fatty acid rich culinary
oils is more harmful compared with those similarly treated oils rich in saturated fatty acids and monounsaturated fatty acids. 相似文献
6.
李霞冰 《中国科学院研究生院学报》1985,23(4):259-263
This paper deals with the constituents of fatty acids from the seed oils of
Pinaceae in China, including 30 species belonging to 8 genera. Discovered are three
characteristic fatty acids, i.e. Cis-5,9-octadecadienoic acid, Cis-5,9,12-octadecatrienoic acid
and Cis-5, 11, 14-eicosatrienoic acid. Based on the data obtained, the distribution of the
three characteristic fatty acids in Pinaceae of China is also discussed. 相似文献
7.
P. Chandran N. K. Kuchhal P. Garg C. S. Pundir 《Indian journal of clinical biochemistry : IJCB》2007,22(2):145-150
Chemical composition of gall stones is essential for aetiopathogensis of gallstone disease. We have reported quantitative
chemical analysis of total cholesterol bilirubin, calcium, iron and inorganic phosphate in 120 gallstones from haryana. To
extend this chemical analysis of gall stones by studying more cases and by analyzing more chemical constituents. A quantitative
chemical analysis of total cholesterol, total bilirubin, fatty acids, triglycerides, phospholipids, bile acids, soluble proteins,
sodium potassium, magnesium, copper, oxalate and chlorides of biliary calculi (52 cholesterol, 76 mixed and 72 pigment) retrieved
from surgical operation of 200 patients from Haryana state was carried out. Total cholesterol as the major component and total
bilirubin, phospholipids, triglycerides, bile acids, fatty acids (esterified), soluble protein, calcium, magnesium, iron,
copper, sodium, potassium, inorganic phosphate, oxalate and chloride as minor components were found in all types of calculi.
The cholesterol stones had higher content of total cholesterol, phospholipids, fatty acids (esterified), inorganic phosphate
and copper compared to mixed and pigment stones. The mixed stones had higher content of iron and triglycerides than to cholesterol
and pigment stones. The pigment stones were richer in total bilirubin, bile acids, calcium, oxalate, magnesium, sodium, potassium,
chloride and soluble protein compared to cholesterol and mixed stones. Although total cholesterol was a major component of
cholesterol, mixed and pigment gall stone in Haryana, the content of most of the other lipids, cations and anions was different
in different gall stones indicating their different mechanism of formation. 相似文献
8.
This study was undertaken to evaluate the effects of n-3 fatty acid supplementation on blood glucose, lipid profile and cytokines
in humans. Twenty adult healthy subjects were supplemented with 1g/day fish oil concentrate capsules for 2 weeks. Fasting
blood samples were taken at baseline and again after 2 week intervention. Fish oil supplementation significantly lowered fasting
serum concentrations of total cholesterol, triacylglycerol, very low density lipoprotein and low density lipoprotein (P <
0.01). Also high density lipoprotein increased significantly compared with baseline value (P < 0.01). Fish oil did not significantly
altere the fasting blood glucose concentration but significantly reduced the serum cytokines (P < 0.001). This study demonstrated
that eicosapentanoic acid (EPA) and docosahexaenoic acid (DHA) supplementation altered serum lipids, lipoprotein and cytokine
concentrations in normal human subjects even at the small dose of 660 mg of EPA plus DHA per day. It appears that EPA plus
DHA can be a safe and perhaps beneficial supplement to inflammatory diseases. 相似文献
9.
Angela Godwin H. Ramachandra Prabhu 《Indian journal of clinical biochemistry : IJCB》2006,21(1):202-204
Fish and fish oils are the richest sources of ω-3 fatty acids. However, they are susceptible to lipid peroxidation due to
their high degree of unsaturation. In the present study, the level of thiobarbituric acid reactive material in various fish
oils available in the market with and without added Vitamin E was determined. The peroxide levels in fish oil heated to food
frying temperature of 180°C and the effect of addition of vitamin E has also been studied. The results indicate that the peroxide
levels in almost all the products available in the market were abnormally high irrespective of their Vitamin E content. This
might be due to the inefficient methods used for processing and storage of fish oils. Addition of vitamin E was found to have
a significant effect in lowering the rate of peroxidation of fish oil during thermal stress, showing that association of antioxidants
with ω-3 fatty acids lowers the rate of lipid peroxidation. 相似文献
10.
Karen M. D'Souza Tester E. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2007,22(2):84-89
The sarcoplasmic reticulum was isolated and characterized in the pure form from normal caprine (Capra hircus) hearts. Under ischemic condition, fatty acids like palmitic acid, palmitoyl carnitine and oleic acid accumulatein vivo. These are known to have a detrimental effect on membrane components. The effects of simulated ischemia on the Ca2+-ATPase were studied using these fatty acidsin vitro at concentrations at which they occurin vivo in the ischemic heart. All three fatty acids inhibited Ca2+-ATPase activity. At lower concentrations, palmitoyl carnitine was the most potent, followed by oleic acid while palmitic
acid displayed the least potency. Electron microscopy studies with fatty acids showed morphological disruptions in SR vesicles.
The decline in Ca2+-ATPase activity could be attributed to the change in membrane morphology. 相似文献
11.
豆科七种野生植物籽油脂肪酸研究 总被引:1,自引:0,他引:1
采用毛细管气相色谱法,对青海境内的豆科七个属的七种野生植物种子的含油率,理化常数和脂肪酸组成及相对含量进行了测定,结查表明,所测植物籽油中,主要为棕榈酸,硬脂酸,油酸,亚油酸和亚麻酸。 相似文献
12.
天仙子籽油中脂肪酸组成的研究 总被引:4,自引:0,他引:4
采用气相色谱法对天仙子籽油中脂肪酸的组成及相对含量进行了研究。鉴定了油酸、亚油酸等十种脂肪酸、其中油酸含量为16.32%,亚油酸含量高达74.81%。 相似文献
13.
Surekha Bhat Guruprasad Rao K. Dilip Murthy P. Gopalakrishna Bhat 《Indian journal of clinical biochemistry : IJCB》2008,23(2):191-194
High ambient temperature has been reported to increase oxidative stress by increasing lipid peroxidation and decreasing antioxidant
defence in transition dairy cows. It is also known to cause an increase in plasma cortisol levels in goats, European hedgehog
and human volunteers. High levels of glucocorticoids have been reported to decrease blood glutathione and erythrocyte superoxide
dismutase activity in rats. Although institutional animal houses in research laboratories of developed countries maintain
animals in air-conditioned rooms at constant temperature, the same is not true of animal houses in the developing countries
especially those belonging to smaller institutions and this could affect the results of the experiments being conducted on
these animals. The present research study was done to assess the effects of seasonal variations on the status of erythrocyte
oxidative damage, antioxidant defence and plasma cortisol levels in adult female Wistar rats. Rats were kept in their home
cages and were left in non-air-conditioned procedure rooms in two different seasons, Hot season (March-May) and Cool season
(June to September). Erythrocyte Thiobarbituric acid reactive substances and plasma cortisol levels were significantly increased
in rats exposed to high ambient temperature and humidity of the hot season as compared to the rats of the cool season. Erythrocyte
reduced glutathione levels, erythrocyte glutathione peroxidase and superoxide dismutase activities were significantly decreased
in the hot season group of rats. The results of our experiments showed that exposure of adult female Wistar rats to high ambient
temperature and humidity of the hot season increases neuroendocrine stress, oxidative stress and decreases antioxidant defence
in them. 相似文献
14.
22 kinds of seed oils were extracted from 8 genera of the family Ulmaceae in China
The seed oils were examined for their characteristics and fatty acid compositions by
gas liquid chromatography. The fatty acid compositions of these oils were found to
fall into two classes. Some genera (such as Ulmus, Zelkova) contain mainly
lower saturated acids, in which the chief acid is capric acid 10:0, while the genera
(such as Celtis, Pteroceltis, Aphananthe, Trema, Gironniera) contain mainly unsaturat-
ed acids, in which the chief acid is linoleic acid 18:2. Hemiptelea davidii (Hance)
Planch contain however either certain amount of short-chain saturated acids or higher
unsaturated acids, it appears a intermediate genus between the two classes. According
to the component acids we support that the Ulmaceae be split into two subfamilies.
The genera arrangement based on the component acids corresponds basically with the
view based on mophological characters and flavonoids found in leaves of Ulmaceae, but
there are some discrepancies in certain genera, for example, the Aphananthe should beplaced in Celtoid instead of Ulmoid by the present study. 相似文献
15.
N. Pavithra Priyanka S. Bannikoppa Sheila Uthappa Anura V. Kurpad Indu Mani 《Indian journal of clinical biochemistry : IJCB》2018,33(3):290-296
Changes in plasma fatty acid (FA) composition and desaturase activities are observed in metabolic syndrome (MS). However, whether these changes are a reflection of dietary intakes of fats and FAs is not well established. The current study was aimed at assessing plasma FA composition and desaturase enzyme activities as biomarkers of dietary intakes in subjects with MS. Case control study was done on 41 MS patients and was compared with age matched 45 controls. Dietary intakes, anthropometric and clinical parameters were measured. FA composition was analysed using gas chromatography-flame ionisation detector and desaturase enzyme activities were estimated as ratios of product to precursor FAs. Higher levels of 14:0, 16:0, 16:1, 18:1, D9D-18 activity and lower levels of 18:0 and 18:2 n-6 were seen in MS group when compared to controls (p < 0.05). Strong positive correlations were seen between plasma triglyceride (TG) levels and 14:0, 16:0, 16:1, 18:1, total saturated fatty acid, total monounsaturated fatty acid, and D9D activities, while 18:0, 18:2 n-6 and total polyunsaturated fatty acid were negatively correlated with TG. Positive correlations were seen between plasma 14:0, 18:1 and D9D-18 activity with total energy intake and carbohydrate (CHO) intakes but not with fat intake. Plasma FA profile appears to be a better index of total energy intake and CHO intake than fat intake, suggesting it might be a good reflection of endogenous FA metabolism. Changes in FA composition may therefore serve as an early index of dysregulation of FA metabolism, resulting in increased risk of MS. 相似文献
16.
K Sudha Ashalatha V Rao S. N. Rao Anjali Rao 《Indian journal of clinical biochemistry : IJCB》2002,17(1):34-41
Oxidative stress is implicated in the etiopathogenesis of a variety of human diseases. Therefore, in the present study, erythrocyte
lipid peroxidation, percentage hemolysis, antioxidant enzymes viz., glutathione reductase, glutathione peroxidase, superoxide
dismutase and plasma antioxidants viz., ceruloplasmin, vitamins A,E and C have been determined in 19 patients with tubercular
meningitis (TBM) and 50 normals. Six patients who were treated with antibiotics were considered for the follow up. The statistical
analysis was carried out by Mann Whitney U test and Wilcoxon rank sum test. Lipid peroxidation (P<0.02), percentage hemolysis
(P<0.001) and plasma ceruloplasmin (P<0.0001) of TBM patients were significantly higher, whereas erythrocyte glutathione reductase
(P<0.05) and plasma antioxidant vitamins A, E and C (P<0.01, P<0.05 respectively) were significantly lower than those of the
controls. In the follow up patients the glutathione reductase and catalase levels were significantly high (P<0.05) compared
to their pre-treated condition. Vitamin C and E levels have attained normal range. This study indicated that the blood antioxidant
status of TBM patients which was low compared to controls improved after treatment, suggesting the role of free radicals in
TBM. 相似文献
17.
长期施肥对农田黑土微生物群落的影响 总被引:1,自引:0,他引:1
研究不同磷肥处理农田黑土基本理化性质、磷脂脂肪酸、酸(碱)性磷酸酶、微生物量碳或氮等变化规律。结果表明,有机肥-化肥配施明显提高土壤有机质、总氮以及速效氮、磷、钾等养分含量,进而促进微生物量碳、微生物量碳/有机质比值、磷酸酶活力以及各菌群磷脂脂肪酸含量显著增加。长期氮磷钾配施土壤有机质活性严重退化、不利于土壤微生物生长,而单施磷肥或氮磷肥配施对微生物活力与结构影响较小。有机肥处理中单烯不饱和/饱和脂肪酸比值>1,远高于其他化肥处理,可用来指示有机质质量与可用性;而支链/单烯不饱和脂肪酸与单烯不饱和/饱和脂肪酸比值呈极显著负相关,表明G+菌对养分胁迫抗性强于G-菌。磷脂脂肪酸PCA表明,有机肥与化肥处理微生物群落结构显著不同。个别脂肪酸载荷值分析表明,真菌受无机肥施用影响显著;而G+菌更易受有机肥影响。 相似文献
18.
Hilda Priya D'Souza H. Ramachandra Prabhu 《Indian journal of clinical biochemistry : IJCB》2006,21(2):138-141
The beneficial effects of ∈-3 fatty acids on human health have been well documented. Fish and fish oils are the richest sources
of ∈-3 fatty acids. However, due to their high degree of unsaturation, they are highly susceptible to lipid peroxidation.
Regular consumption of peroxidised oils may represent a risk factor for the induction and development of atherosclerosis.
In view of the above reports, it was considered necessary to study the effects of tumeric on fish lipid peroxidation during
standard cooking practices and on time-dependent changes in the peroxidation of fish homogenate. The antioxidant effect of
α-tocopherol was also studied to confirm the relevance of the study. The results suggest that turmeric may be considered as
a safe, cheap and readily usable antioxidant for food preparations. 相似文献
19.
Sanja Grkovic Rajko Nikolic Maja Djordjevic Ljubomir Stojanov Snezana Zivancevic-Simonovic Gordana Djordjevic-Denic Bozica Kecman 《Indian journal of clinical biochemistry : IJCB》2007,22(2):118-122
X-linked adrenoleukodistrophy is a severe neurodegenerative disorder with impaired very long chain fatty acid metabolism.
The disease associated ABCD1 gene encodes a peroxisomal membrane protein which belongs to the superfamily of ATP-binding cassette
transporters. We investigated eight male X-ALD patients diagnosed among 142 suspected patients referred for investigation.
Plasma levels of very long chain fatty acids were measured at our laboratory using capillary gas chromatography. Eight cases
of childhood X-ALD were diagnosed. This is the first published series of Serbian patients with X-ALD. In addition, diagnosis
identifies carriers, which could be benefit for genetic counselling and prenatal diagnosis. 相似文献
20.
Oxidative stress in Parkinson’s disease 总被引:1,自引:0,他引:1
Shashikant Nikam Padmaja Nikam S. K. Ahaley Ajit V. Sontakke 《Indian journal of clinical biochemistry : IJCB》2009,24(1):98-101
Oxidative stress contributes to the cascade, leading to dopamine cell degeneration in Parkinson’s disease. However, oxidative
stress is intimately linked to other components of the degenerative process, such as mitochondrial dysfunction, excitotoxicity,
nitric oxide toxicity and inflammation. It is therefore difficult to determine whether oxidative stress leads to or is a consequence
of, these events. Oxidative stress was assessed by estimating lipid peroxidation product in the form of thiobarbituric acid
reactive substances, nitric oxide in the form of nitrite & nitrate. Enzymatic antioxidants in the form of superoxide dismutase,
glutathione peroxidase, catalase, ceruloplasmin and non enzymatic antioxidant vitamins e.g. vitamin E and C in either serum
or plasma or erythrocyte in 40 patients of Parkinson’s disease in the age group 40–80 years. Trace elements e.g. copper, zinc
and selenium were also estimated. Plasma thiobarbituric acid reactive substances and nitric oxide levels were Significantly
high but superoxide dismutase, glutathione peroxidase, catalase, ceruloplasmin, vitamin-E, vitamin-C, copper, zinc and selenium
levels were significantly low in Parkinson’s disease when compared with control subjects. Present study showed that elevated
oxidative stress may be playing a role in dopaminergic neuronal loss in substentia nigra pars compacta and involved in pathogenesis
of the Parkinson’s disease. 相似文献