蒌叶提取物增强5-氟尿嘧啶对结肠癌细胞HT29和HCT116的生长抑制作用(英文)

研究目的:探讨蒌叶(PB)提取物对5-氟尿嘧啶(5-FU)抑制结肠癌细胞HT29和HCT116生长的影响。研究方法:HT29和HCT116细胞分别给予PB、5-FU以及两种药物联合治疗24小时,应用等效线图法分析PB和5-FU的药效学相互作用,Annexin V/PI染色法检测HT29和HCT116细胞的凋亡情况,高效液相色谱法排除PB和5-FU间任何可能的相互化学作用。重要结论:联合PB,低剂量5-FU可以在短时间内起到细胞毒作用,而单独应用PB或5-FU治疗较联合治疗可以诱导更多细胞发生凋亡。进一步采用等效线图法分析显示PB和5-FU的联合作用在抑制结肠癌细胞HT29和HCT116的生长中分别体现出协同和拮抗作用。因此可以认为在HT29细胞中,PB使得较低剂量5-FU发挥最大抑制结肠癌细胞生长效果,然而在HCT116细胞中,PB没有显著降低5-FU的药物浓度,说明PB和5-FU的相互作用不仅仅体现在诱导细胞凋亡方面。     

5-氟尿嘧啶对人胃癌细胞超微结构和GSTπ表达的影响

目的:探讨5-氟尿嘧啶对人胃癌细胞的结构和GSTπ表达的影响。方法:在人胃癌BGC-823细胞株培养液中加入5-氟尿嘧啶,继续培养5h或10h,然后用HE、免疫组化染色和铀-铅染色。光镜、电镜下观察。结果:10h组胃癌细胞外型畸变、细胞器破坏严重;GSTπ灰度发生变化。结论:5-氟尿嘧啶作用10h对人胃癌细胞超微结构及GSTπ基因表达有明显影响。     

2-噻吩甲酸（5-氟尿嘧啶-1-基）甲酯的合成、晶体结构和热性质

报道了一种新的5-氟尿嘧啶衍生物2-噻吩甲酸（5-氟尿嘧啶-1-基）甲酯的合成.用1HNMR、13CNMR、IR对其进行了结构表征,通过单晶X射线衍射确定了晶体结构,同时分析了热性质.晶体属单斜晶系,空间群为P2/c,晶胞参数为：a=9.6134（14）,b=4.8381（7）,c=24.534（4）,α=90°,β=97.289（3）°,γ=90°;Z=4,F（000）=552,R1=0.0616.     

Hydroxychavicol,a polyphenol from Piper betle leaf extract,induces cell cycle arrest and apoptosis in TP53-resistant HT-29 colon cancer cells

This study aims to elucidate the antiproliferative mechanism of hydroxychavicol(HC).Its effects on cell cycle,apoptosis,and the expression of c-Jun N-terminal kinase(JNK)and P38 mitogen-activated protein kinase(MAPK)in HT-29 colon cancer cells were investigated.HC was isolated from Piper betle leaf(PBL)and verified by high-performance liquid chromatography(HPLC),nuclear magnetic resonance(NMR),and gas chromatography-mass spectrometry(GC-MS).The cytotoxic effects of the standard drug 5-fluorouracil(5-FU),PBL water extract,and HC on HT-29 cells were measured after 24,48,and 72 h of treatment.Cell cycle and apoptosis modulation by 5-FU and HC treatments were investigated up to 30 h.Changes in phosphorylated JNK(pJNK)and P38(pP38)MAPK expression were observed up to 18 h.The half maximal inhibitory concentration(IC₅₀)values of HC(30μg/mL)and PBL water extract(380μg/mL)were achieved at 24 h,whereas the IC₅₀of 5-FU(50μmol/L)was obtained at 72 h.Cell cycle arrest at the G0/G1 phase in HC-treated cells was observed from12 h onwards.Higher apoptotic cell death in HC-treated cells compared to 5-FU-treated cells(P<0.05)was observed.High expression of pJNK and pP38 MAPK was observed at 12 h in HC-treated cells,but not in 5-FU-treated HT-29 cells(P<0.05).It is concluded that HC induces cell cycle arrest and apoptosis of HT-29 cells,with these actions possibly mediated by JNK and P38 MAPK.     

Ursolic acid inhibits proliferation and induces apoptosis of HT-29 colon cancer cells by inhibiting the EGFR/MAPK pathway

rectal cancer.     

安陆白花菜5S和45SrDNA染色体定位

安陆白花菜是湖北地方性经济作物,市场价值和药用价值都较高,本研究采用荧光原位杂交技术,对5SrDNA和45SrDNA在安陆白花菜有丝分裂中期染色体上的物理位置进行了定位分析。结果显示5SrDNA杂交信号l对,位于第11号染色体上长臂;45S杂交信号1对,位于第16号染色体上长臂。安陆白花菜的染色体数目为34条,染色体基数17,2A核型,核型公式为K（2n）=34=14m（2SAT）＋20sm（2SAT）,其中第11、16号染色体长臂末端带有随体,平均臂比1．733,最长染色体／最短染色体1．478,臂比大于2的比率29．41,核型不对称系数62．73,染色体相对长度组成为2L＋12M2＋20M1。