扬子鳄心脏P物质免疫反应纤维和细胞的分布

用免疫组织化学法和异硫氰酸标记的间接免疫荧光方法观察了扬子鳄心脏内Ｐ物质免疫反应阳性神经纤维和细胞的分布。结果发现ＳＰ－ＩＲ纤维分布于心室、心房、静脉窦、主动脉瓣处;右心房见散在阳性细胞及神经节。ＩＲ纤维呈“串珠状”、线状或交织成网络状,主要分布于肌层及外膜,多伴血管走行。     

金华猪种心脏的组织学研究

目的 :通过对 60例金华猪种心脏 (宰杀 4 h内 )的组织切片研究 ,观察其心内膜、心肌层及瓣膜的组织结构特点 ,为转基因猪心脏的人体移植提供组织学基础资料 .方法 :将 50例新鲜金华种猪心脏清洗后取材 ,将取下的左心室、右心房 ,二尖瓣组织经常规固定后用石蜡包埋 ,组织切片经苏木素一伊红染色后在低、高倍显微镜下观察 .结果 :金华猪心的组织结构与人心有诸多相似 ,适合于供人体移植 ,也有下列几方面差异应引起注意 :1猪心的心内膜内皮下层主要由疏松结缔组织和少量的平滑肌纤维组成 ,较人之心内皮下层厚而松散 ;2猪心的束细胞较正常人的束细胞少而大 ;3猪心的肌纤维粗细相对均匀 ,但其间闰盘明显少于人心的心肌     

高血压病左室肥厚与部分神经体液因素的相关性

为探讨高血压病左室肥厚与神经体液因素及室性早博（ＶＰＢ）之间的关系,将１８０例病人分３组：高血压病伴左室肥厚６０例为Ａ组;单纯高血压病６０例为Ｂ组;其他原因所至的左室肥厚６０例为组。对比观察心率为异、肾素（Ｒｅｎ）、血管紧张素（ＡＴ－Ⅱ）、醛固酮（Ａｌｄ）、胰岛素峰值（Ｉｎｓ）２４小时动态心电图和血压等指标。结果表明：（１）Ａ组病人交感神经张力明显增高,迷瞳神经活性下降不明显;交感神经张力增高与室     

聚合物电解质化学与电化学稳定性的研究

运用ＩＲ、ＤＳＣ和电化学测试等手段,对ＰＶＢ＋ＬｉＣｌＯ４＋ＰＣ组成的聚合物 电解质的化学和电化学稳定性作了初步的研究。本实验制备的聚合物电解颀在２５０℃以下的温度范围内,具有较好的热稳定性。由Ｐｔ／ＰＶＢ＋ＬｉＣｌＯ４＋ＰＣ／Ｌｉ组成的测试电池的开路电压为２．３Ｖ（ｖｓ．Ｌｉ＾＋／Ｌｉ）,并随时间延长而减小。聚合物电解质阳极过程的稳定性受到阴离子氧化电位的限制,在０～４．２Ｖ（ｖｓ．Ｌｉ＾＋／Ｌ     

聚合酶链反应检测乙型肝炎患者血清HBVDNA与ELISA检测HBeAg、抗HBe比较研究

应用聚合酶链反应（ＰＣＲ）检测了１５３例各型乙型肝炎患者的血清ＨＢＶＤＮＡ，并与ＥＬＩＳＡ法检测的血清ＨＢｅＡｇ和抗ＨＢｅ结果比较．结果ＨＢｅＡｇ阳性８９例，ＰＣＲ法ＨＢＶＤＮＡ检出率为９２．１３％；抗ＨＢｅ阳性２７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为５１．８５％；ｅ系统用性３７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为４０．５４％．提示抗ＨＢｅ血清学转换不能作为乙型肝炎病毒复制与非复制、病变活动与静止的唯一指标，而应该用或同时用ＨＢＶＤＮＡ作为指标才可靠．     

常规业绩评价方法中存在的问题与解决方法

项目执行后,在进行业绩评价时,我们曾在投资预测与决策中使用过的净现值（ＮＰＶ）、内部收益率（ＩＲＲ）、净年值（ＮＡＶ）、投资回收期（ＩＲＰ）等以现金流量为基础的指标似乎被遗忘了,取而代之的是投资报酬率（ＲＯＩ）、资产周转率（ＡＴＲ）、权益净利率（ＲＯＥ）等一系列利润或收益性质的指标,这便违背了“预算和评价同口径”的最基本原则。而在本文中所提出的两个指标：现金流量回收率（ＣＦＲＲ）和剩余现金流量（ＳＣＦ）较好地克服了这种不足。     

Iodogen法碘化标记单克隆抗体通用技术研究

报道了Ｉｏｄｏｇｅｎ法碘化标记单克隆抗体（单抗）的最佳条件和对标记单抗免疫活性影响的实验结果，结果表明，当Ｉｏｄｏｇｅｎ：ＭｃＡｂ＝１：４缓冲液ｐＨ＝７．４，旋涡搅拌１０ｍｉｎ时，标记率、放化纯和峰值百分率均较高，标记单抗的免疫活性与标记前比较没有统计学差异（Ｐ＞０．０５）。￣１３１Ｉ－ＨＣＭ８３和￣１２５Ｉ－ＶＣＭＣ与阳性对照结合率为３８．２％和３０．１％，Ｐ／Ｎ为９．１和３０．１。此通用技术具有推广应用价值。     

阿霉素对兔血清NO含量和心肌细胞肌浆网Ca2+-ATP酶活性的影响

为探讨阿霉素（ＡＤＲ）对心脏的慢性毒性作用,将新西兰纯种兔１８只随机分成ＡＤＲ组和对照组。ＡＤＲ组每周静脉注射一次生理盐水配制的ＡＤＲ,每公斤体重２ｍｇ,共８次。对照组仅注射生理盐水。于末次注射后３周,测量兔的心输出量（ＣＯ）、颈动脉压（ＢＰ）、劲动脉平均压（ＭＡＰ）、左心室收缩压（ＬＶＳＰ）和左心室舒张末压（ＬＶＥＤＰ）及心肌肌浆网（ＳＲ）的Ｃａ＾２＋－ＡＴＰ酶活性均明显低于对照组;ＬＶＥＤＰ和     

杂交稻中共显性RAPD标记及产量性状基因的检测

ＲＡＰＤ标记是利用含有１０（或９）个碱基的随机引物（单引物）,通过聚合酶链式反应（ＰＣＲ）对模板ＤＮＡ进行随机扩增,比较不同基因组ＤＮＡ的差异,ＲＡＰＤ标记具有样品用量少,灵敏度高,检测容易等优点,因而比ＲＦＬＰ更易于应用,但ＲＡＰＤ是显性标记,在分离群体中不能区别纯合显性和杂合显性个体。     

抗人甲胎蛋白（AFP）单克隆抗体的制备及其特性研究

采用亲和层析法纯化的ＡＦＰ抗原免疫的ＢＡＬＢ／ｃ鼠脾细胞与ＳＰ２／０小鼠骨髓瘤细胞系融合,获三株分泌抗人ＡＦＰ单克隆抗体（ＭｃＡｂ）的杂交瘤细胞系．用ＥＬＩＳＡ阻断试验和夹心ＥＬＩＳＡ试验作特异性鉴定,杂交瘤细胞株染色体的众数为１０４条,三株细胞系ＭｃＡｂ均属小鼠ＩｇＧ１型．经体外连续传代３个月及冻存一年复苏后均能持续分泌抗ＡＦＰ抗体     

Evaluation of plasma hormone concentrations using Enzyme-Immunoassay/Enzyme-linked Immunosorbent assay in healthy Indian men: Effect of ethnicity

The study involved three ethnic groups of India; Rajputs, Gorkhas and South-Indians. Each group consisted of ∼40 healthy, male soldiers between 20–50 years. The reference ranges for cortisol, testosterone, prolactin, arginine vasopressin and proAtrial natriuretic peptide_1–98 were determined using Enzyme-Immunoassay (EIA) while plasma levels of thyroid-stimulating hormone, triiodothyronine, free-triiodothyronine, thyroxine and freethyroxine were measured using Enzyme-linked immunosorbent assay (ELISA). The results indicated that plasma hormone concentrations were within physiological range and inter-ethnic differences were most prominent between north- (Rajputs and Gorkhas) and south- Indians. In comparison to Radioimmunoassay, the EIA method for prolactin, thyroid-stimulating hormone, free-thyroxine gave higher values while the ELISA method for triiodothyronine, free-triiodothyronine, and thyroxine gave lower values. These differences are due to differences in assay standards and design.     

Integrated carbon fiber electrodes within hollow polymer microneedles for transdermal electrochemical sensing

In this study, carbon fiber electrodes were incorporated within a hollow microneedle array, which was fabricated using a digital micromirror device-based stereolithography instrument. Cell proliferation on the acrylate-based polymer used in microneedle fabrication was examined with human dermal fibroblasts and neonatal human epidermal keratinocytes. Studies involving full-thickness cadaveric porcine skin and trypan blue dye demonstrated that the hollow microneedles remained intact after puncturing the outermost layer of cadaveric porcine skin. The carbon fibers underwent chemical modification in order to enable detection of hydrogen peroxide and ascorbic acid; electrochemical measurements were demonstrated using integrated electrode-hollow microneedle devices.     

Effect of caffeine on the risk of coronary heart disease— A re-evaluation

The effect of caffeine intake on the risk of coronary heart disease was studied. Twenty-one rats used were randomly divided into three experimental groups, the first group served as the control while the second and third groups were administered caffeine orally at doses of 10mg/kg body weight and 20mg/kg body weight respectively for fourteen days. Caffeine, at 10mg/kg body weight, significantly increased (P<0.05) serum LDL- cholesterol concentration and coronary heart disease risk ratio while it significantly reduced (P<0.05) serum triacylglycerol concentration when compared with controls. At 20mg/kg body weight, caffeine significantly increased (P<0.05) coronary heart disease risk ratio while it significantly reduced (P<0.05) serum HDL-cholesterol concentration and serum triacylgycerol concentration when compared with controls. No dose response effect was observed possibly suggestive of a threshold effect. These results suggest that caffeine predisposes consumers of caffeine containing beverages to coronary heart disease.     

冠心病患者体外血栓和血小板聚集功能测定的初步探讨

对７１例健康人与８３例冠心病患者体外血栓、血小板聚集功能测定比较，结果证明冠心病患者的体外血栓和血小板聚集明显高于健康人（Ｐ＜０．０１）。     

基于开放标准的数字图书馆检索接口

数字图书馆是基于Internet的分布式信息系统，分布式检索的实现是系统的关键因素之一。本文描述了数字图书馆系统的分布式检索接口，分析了开放标准Web服务和J2EE在数字图书馆系统结构设计中的应用优势，并通过实例阐述如何为已有的数字图书馆检索系统提供一个Web服务节点。     

Protective Role of <Emphasis Type="SmallCaps">l</Emphasis>-Arginine Against Free-Radical Mediated Oxidative Damage in Patients with Unstable Angina

Unstable angina is a critical condition of heart resulting from narrowing of vessels supplying blood to heart. Ischemia of the myocardium leads to oxidative stress and severe tissue damage. The objective of the present study was to determine the effect of l-arginine administration on the oxidant–antioxidant homeostasis which otherwise gets imbalanced in patients with cardiovascular diseases. The results obtained, show improvement in the oxidant–antioxidant levels of the subjects upon incorporation of l-arginine. Our findings suggest that supplementation of l-arginine along with regular anti-anginal therapy may be beneficial to the patients of unstable angina.     

Defect in mitochondrial functions in damaged human mitral valve

Mitochondrial diseases are a heterogeneous group of disorders in which a primary mitochondrial dysfunction is proven by morphological, biochemical, and genetic examinations. The mitral valve has important function in the regulation of blood flow from one chamber to another. Often, the mitral valve becomes abnormal with age, in Rheumatic fever or it is abnormal from birth (Congenital) or it can be destroyed by infection i.e. bacterial endocarditis and needs replacement. Myocardial function depends on energy produced by mitochondria and in any of these disease conditions, mitochondrial functions and enzyme activities may be impaired. With this in view, we analyzed the relationship between preoperative clinical conditions (as per New York heart Association) and extent of mitochondrial enzyme activities in damaged Human mitral valve in two types of heart disease such as Rheumatic Heart Disease (RHD) and Bacterial Endocarditis (BE). Thirty nine Patients undergoing cardiopulmonary bypass (CPB) for routine valvular heart surgery were included in the study. Controls included 11 normal porcine mitral valve samples without any evidence of heart disease. Mitochondrial enzymes like cytochrome oxidase (COX), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), citrate synthase (CS) and ATPase were determined. Mitochondrial COX, SDH, CS and Total ATPase activities were significantly decreased in disease condition like BE and RHD when compared with control (P<0.001). On the other hand as per New York Heart Association (NYHA) preoperative clinical classification, all the mitochondrial enzymes were significantly (p<0.05) impaired in class IV as compared with NYHA class I, II and III. Present study shows that impairment in the mitochondrial enzymes activities are more pronounced in bacterial endocarditis (BE). It also indicates that damage to mitochondrial enzymes are most pronounced in NYHA class IV.     

Risk factors for coronary heart disease in type II diabetes mellitus

Cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. An attempt has been made to evaluate the risk factors for coronary heart disease in type II diabetics. In the present study the levels of fasting and postprandial plasma glucose, total cholesterol, low density lipoproteins, triglycerides were high and the levels of high density lipoproteins were low in the type II diabetics compared to controls. The markers of free radical induced injury i.e. malondialdehyde and nitrite/nitrate were high while total antioxidant status a marker for antioxidant protection against reactive oxygen species was low in diabetics compared to controls. The study therefore suggests the importance of assessing these markers of oxidative stress and antioxidant capacity along with the other routine investigations in diabetic patients for initiating antioxidant therapy in addition to primary and secondary preventive measures to mitigate the devastating consequences of diabetes leading to coronary heart disease.