尖海龙提取物对肿瘤细胞系SK-RB-3的杀伤作用研究

制备尖海龙提取物,再将其作用于SK-RB-3细胞系,从细胞形态变化、细胞计数、细胞分裂指数变化观察体外药效。结果表明,尖海龙提取物可改变SK-RB-3细胞的正常生活形态,又可显著抑制肿瘤细胞的增殖,减少分裂指数,从而证实尖海龙提取物具有抗癌活性,其在癌症的治疗与预防方面具有使用价值。     

锁阳多糖对Hela和A549细胞增殖抑制作用的比较

探讨锁阳多糖对人宫颈癌Hela细胞和人肺癌A549细胞的增值抑制作用,在细胞浓度为10~5个/m L中分别加入0.05、0.1、0.2、0.4和0.8mg/m L的锁阳多糖,培养120h后检测细胞生长抑制率、细胞存活率观察细胞形态的变化.结果表明:当锁阳多糖浓度为0.8mg/m L,作用时间为120h时,对Hela细胞和A549细胞的增殖抑制率分别为63.53%和67.62%,其存活率分别为37.5%和33.4%,说明锁阳多糖对Hela细胞和A549细胞均有抑制作用,且对A549细胞增殖抑制作用强于Hela细胞.     

刺五加注射液对 Hela 细胞抑制作用的实验研究

目的:探讨刺五加注射液对人宫颈癌细胞株Hela体外增殖的抑制作用。方法:采用MTT法测定了不同浓度的刺五加注射液对人宫颈癌细胞株Hela的体外抑制作用,倒置显微镜观察细胞形态变化。结果:刺五加注射液浓度越高对Hela细胞抑制作用越好,作用时间越长抑制效果越强,量效关系和时效关系良好;形态学观察到细胞凋亡。结论:刺五加注射液对人宫颈癌细胞株Hela的细胞增殖有较强的抑制作用。     

爸爸是个小肉瘤?

海象从师范学校毕业以后当了一名小学老师，班上的海龙呀、琵琶鱼呀，不是迟到就是和别的小同学闹别扭，多次批评教育仍不见效，他想和家长联系一下，共同教育。于是，不顾工作一天的辛劳，叩响了海龙家的大门。“海龙妈妈，我是小海龙的老师呀！”开门的人先让海象老师吃了一惊：海龙“妈妈”身材并不是很高很高的，只有20厘米左右，而且细得像一条小蚯蚓，哪里有一点龙的风采啊！“老师，我不是海龙妈，是海龙爸，有什么事尽管说！”“这……这怎么可能？”海象老师支吾着，揉了揉眼睛，迷惑不解地说。“在产卵的时候，海龙妈妈把卵产在我…     

牛蒡抗氧化作用的研究

通过在四种油脂中添加适当的牛蒡乙醇提取物，在不同时间测定油脂POV值的方法，研究了牛蒡对食用油脂的抗氧化性，结果发现对菜籽油、花生油、酥油、猪油均有一定的抗氧化作用，对其作用机理作了初步的分析。     

襄麦冬粗提物抗菌活性初步研究

以蒸馏水、甲醇、乙酸乙酯为溶剂,提取襄麦冬活性物质,并分别测定提取物对以下5种病原真菌：立枯丝核菌Rhizoctonia solani,灰葡萄孢菌Botrytis cinerea,齐整小核菌Sclerotium rolfsii,终极腐霉Pythium ultimum,尖镰孢菌黄瓜转化型Fusarium oxysporum f.sp cucumber菌丝生长和孢子萌发的抑制作用.结果表明襄麦冬的三种溶剂提取物对灰葡萄孢菌、终极腐霉、立枯丝核菌的菌丝生长均具有较强的抑制作用,而上述相同浓度的提取物对齐整小核菌、尖镰孢菌黄瓜转化型的生长未表现出明显的抑制效果.同时,三种溶剂提取物对立枯丝核菌、灰葡萄孢菌、齐整小核菌、终极腐霉、尖镰孢菌黄瓜转化型的孢子萌发均表现出一定的抑制作用.其中,襄麦冬乙酸乙酯提取物较蒸馏水和甲醇提取物对病原真菌的生长和孢子萌发的抑制效果明显.乙酸乙酯提取物相对于蒸馏水、甲醇提取物有较好的抑菌效果.襄麦冬提取物对灰葡萄孢菌、终极腐霉、立枯丝核菌的菌丝生长和立枯丝核菌、灰葡萄孢菌、齐整小核菌、终极腐霉、尖镰孢菌黄瓜转化型的孢子萌发有良好的抑制作用.     

揭开播州土司历史面纱的力作——评李飞等考古学报告《海龙屯》

海龙屯是播州杨氏的军事要塞和统治据点,其一生主要经历了南宋时期的修建、明万历时期的重修和平播战争的破坏。自中国启动土司遗址申遗以来,土司研究进一步深入,有关于土司遗址的考古工作也愈发得到各界的重视。贵州省海龙屯遗址考古团队,自2012年开始历时八年对海龙屯进行了实地考古工作,而于2022年付梓出版的四卷本《海龙屯》,正是这一工作的结晶。《海龙屯》以考古学、历史学等多学科融合的视野,全景式地剖析了海龙屯土司遗址,可谓揭开播州土司历史面纱的力作。     

海兔卵提取物抗肿瘤抗氧化的药理研究

海兔卵乙醇提取物和水提取物均对小鼠的心、肝、紧具有明显的抗脂质过氧化作用，两种提取物均有抑制肉瘤180的瘤体重量，其中乙醇提取物均增加EAC腹水瘤小鼠的存活率、延长小鼠的生命，且乙醇提取物高剂量组的作用有显著性差异．     

生物体液如何维持酸碱平衡

生物细胞对于体液酸碱性的变化非常敏感，当体液PH值偏离其正常范围时，将使细胞代谢发生障碍，甚至有生命危险。本文着重介绍了生物体液如何能抵御外界因素的影响而维持正常的PH值范围。     

脱氧胆酸衍生物的合成、表征及抗癌活性研究

本文以脱氧胆酸为先导化合物,在C-3位导入叠氮基,得到目标化合物DCA-3,将C-12位的羟基氧化成酮,得到目标化合物DCA-6,利用IR、1H NMR等波谱技术对所合成的目标化合物进行了结构表征,并进行了对人乳腺癌细胞(MCF-7)和人子宫颈癌细胞(Hela)的抑制作用测试。结果表明化合物DCA-3对MCF-7和Hela均表现出抑制作用,DCA-6对Hela表现出抑制作用,且目标化合物抗癌活性均优于先导化合物脱氧胆酸。     

我国鲤鲫寄生粘孢子虫名录

报导了我国鲤、鲫寄生粘孢子虫的名录,寄生鲤的粘孢子虫有４１ 种,寄生鲫的粘孢子虫有４７ 种     

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

The osteoinduction of porous biphasic calcium phosphate ceramics (BCP) has been widely reported and documented, but little research has been performed on rodent animals, e.g., mice. In this study, we report osteoinduction in a mouse model. Thirty mice were divided into two groups. BCP materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle, respectively. Five mice in each group were killed at 15, 30, and 45 d after surgery. Sample A and Sample B were harvested and used for hematoxylin and eosin (HE) staining, immunohistochemistry (IHC) staining, and Alizarin Red S staining to check bone formation in the biomaterials. Histological analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two groups. Newly-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation; the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%, respectively. However, we did not see any bone tissue in Sample B until 45 d after implantation. Bone-related molecular makers such as bone morphogenesis protein-2 (BMP-2), collagen type I, and osteopontin were detected by IHC staining in Sample A 30 d after implantation. In addition, the newly-formed bone was also confirmed by Alizarin Red S staining. Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available, our results may contribute to further mechanism research.     

Evaluation of the dust and methanol extracts of Garcinia kolae for the control of Callosobruchus maculatus （F.） and Sitophilus zeamais （Mots）

Insecticidal effects of different doses of the dust and methanol extracts of Garcinia kolae on Callosobruchus maculatus and Sitophilus zeamais were tested. The dust had no significant effect on the two insects, none of them died even at 3 d after treatment. The methanol extracts, however, had rapid lethal effects on both C. maculatus and S. zeamais. The mortality of C. maculatus by the lowest concentration of methanol extracts ranged from 95%-100% whereas in S. zeamais, the mortality ranged from 87.5%-100% and 70%-100% in concentrations of 1 g extract＋3 ml methanol and 1 g extract＋5 ml methanol, respectively, from 24 to 48 h. The least concentration of 1 g extract＋l 5 ml methanol had no significant lethal effect on Sitophilus zeamais.     

舒芬太尼在老年腹腔镜胆囊切除术中的应用

目的：探讨舒芬太尼在老年患者腹腔镜胆囊切除术中的麻醉效果。方法：180例接受腹腔镜胆囊切除术的老年患者随机分为3组：芬太尼组（F组芬太尼用量20μg/kg）,舒芬太尼1组（S1组舒芬太尼用量为0.5μg/kg）,舒芬太尼2组（S2组舒芬太尼用量为1μg/kg）。比较3组患者镇静后10 min时、麻醉诱导即时、气管插管后3 min、二氧化碳气腹、二氧化碳气腹15 min、手术结束时的SBP、DBP。推算MBP、HR、ECG、SpO2。观察患者手术结束时到自主呼吸恢复时间、初醒时间,气导管拔出的时间及拔管时患者SBP、DBP。推算MBP、HR、ECG、SpO2和并发症发生情况。结果：S1组和S2组两组患者在气管插管,二氧化碳气腹前后血流动力学波动较小,而F组患者的波动相对较大。S1,S2组间比较,差异无统计学意义;S1组与F组比较自主呼吸恢复时间、苏醒时间、气导管拔出的时间,差异无统计学意义。拔管时患者SBP、DBP、MBP、HR,S1组血流动力学更加平稳,且术后并发症明显减少,与F组比较,差异有统计学意义;但S2组自主呼吸恢复时间明显延长,部分患者需要加用阿片类药物拮抗药,使用拮抗药后部分患者有烦躁、不自主体动等并发症,与其他两组比较,差异有统计学意义。结论：老年患者腹腔镜胆囊切除术采用舒芬太尼0.5μg/kg,能良好的控制因气管插管,二氧化碳气腹导致的应急反应,维护血流动力学的平稳,同时不影响患者术后的苏醒。     

一种线性可控全集成SiGe BiCMOS驱动放大器

基于Jazz工艺,提出一种线性可控全集成Si Ge Bi CMOS驱动放大器（DRA）,实现多种可调功率增益放大作用。电路采用全差分共射共基结构,通过调节CMOS电流镜偏置电路和Si Ge-HBT管尺寸以及3bit控制位,实现1d B步长的可控增益。仿真结果显示：在10μA的带隙基准电流源以及3.3V的电源电压下,DRA实现八种可调功率增益,其线性度指标即输出1d B压缩点OP1d B〉3d Bm,电路供电电流〈10m A,且电路输入输出匹配良好（S11与S22均小于-19d B）。     

Copper-modified palygorskite is effective in preventing and treating diarrhea caused by <Emphasis Type="Italic">Salmonella typhimurium</Emphasis>

The aim of this research was to develop effective alternative therapies to reduce antibiotic use in animal agriculture. In this study, the efficacy of copper-modified palygorskite (CM-Pal) in preventing diarrhea caused by Salmonella was specifically examined both in vitro and in vivo. The CM-Pal was prepared with palygorskite (Pal) and copper nitrate. The antibacterial activity of the CM-Pal was detected by comparing the differences in cell numbers on plate count agar before and after adding the CM-Pal to Salmonella typhimurium cultures. Seventy ICR mice were then allocated into seven groups. Five groups (the treatment groups) were infected with S. typhimurium by intraperitoneal (i.p.) injection and were given Pal, CM-Pal, montmorillonite powder, gentamicin, and physiological saline, respectively. One group (the prevention group) was given CM-Pal before infection with S. typhimurium. Another group (the uninfected group) was not infected with S. typhimurium. The effects of Pal, CM-Pal, montmorillonite powder, and gentamicin on the treatment or prevention of diarrhea in the mice were examined by stool studies, fecal scoring, and assessment of growth performance and villus height. The CM-Pal had satisfactory anti-bacterial properties in vitro: the antibacterial rate was 100% after 2 h incubation with S. typhimurium NJS1 cultures (1×10⁶ colony-forming units (CFU)/ml). In the in vivo experiment, the CM-Pal exerted superior effects in the treatment and prevention of diarrhea in mice compared with Pal, montmorillonite powder, and gentamicin. In the CM-Pal group, no mice showed signs of diarrhea at 24 h post infection (p.i.), and all mice fully recovered from infection. However, the Pal group, montmorillonite group, and gentamicin group only recovered after 48, 48, and 96 h, respectively. The villus height level in the CM-Pal treatment group recovered at 3 d p.i. However, the recovery time of the other groups was longer (at least 5 d). The CM-Pal prevention group had a better effect on weight gain than the other groups. This study suggested that CM-Pal may be an effective alternative to conventional antibiotics for the treatment and prevention of animal diarrhea caused by Salmonella.     

Ag-TiO2纳米催化剂的合成、表征及环氧化催化性能

用溶胶－凝胶－表面活性剂法合成了Ag-TiO2纳米催化剂，并用FTIR、XRD、TEM、比表面测定对其进行了表征。纳米催化剂的一次粒径为5.9纳米，二次粒径为12．1纳米。实验表明，它对苯乙烯的H2O2环氧化的催化性能较好，对苯乙烯的分子氧环氧化有一定催化性能。     

重庆市北碚区鲤鲫寄生粘孢子虫

记述重庆市北碚区鲤、鲫寄生粘孢子虫 9种 ,即楔形两极虫、多态两极虫、塔形碘泡虫、异样碘泡虫、椭圆碘泡虫、茄形碘泡虫、野鲤碘泡虫、徐家汇尾孢虫、大型粘体虫 .其中塔形碘泡虫、异样碘泡虫、大型粘体虫均为该种的新分布 ;多态两极虫、塔形碘泡虫则分别为该种营养体、孢囊的首次报道     

培养“3S”应用人才实践教学模式的探索与实践——以兰州财经大学为例

“3S”应用技术飞速发展,涵盖了国土、地矿、环保、城建、能源、经济等范围。“3S”产业已经成为一门综合性的新兴产业。现阶段本科生“3S”技术实践课程教学模式与企业实际生产相互脱节,这种现象迫切要求对当前本科生实践课教学进行创新。从应用型人才培养目标出发,分析实验教学中存在的问题,革新“3S”应用型人才培养理念,采用校企联合培养方式,探讨在新培养模式中如何改革“3S”课程教学中的实验教学,提出创新的授课、考核、教学、组织和运行方式,让学生对学科的实践意义有更深层次的了解。对于学生毕业后更好地适应市场需求,具有十分重要的现实意义。同时,也对其他的专业教学改革具有一定的借鉴意义。