基于微阵列技术的缺氧/复氧诱导下血管内皮细胞转录组分析

目的:应用全转录组芯片研究缺氧/复氧诱导下人脐静脉内皮细胞(HUVEC)的转录组轮廓。创新点:血管内皮细胞(VEC)缺氧/复氧损伤被视定为许多生理和病理过程中导致器官功能障碍的重要驱动因素。然而,其详细病理生理机制和基因表达谱信息尚未阐明。本研究首次应用全转录组芯片技术研究VEC缺氧/复氧诱导下的转录组轮廓。方法:采用缺氧孵育3h后复氧1h的HUVEC为缺氧/复氧组,同时常氧孵育的HUVEC为常氧对照组。应用含58 339条探针的全转录组芯片检测每组三个样本。对差异表达基因进行生信分析和功能验证。结论:本研究发现372个有意义的差异表达基因探针。相关基因涵盖多种途径和功能,例如氧自由基的产生、钙超载、炎症、糖脂代谢、内皮细胞增殖、分化、细胞骨架及通透性调节、细胞裂解、凋亡和血管生成。另外,实验进一步表明,差异表达基因pleckstrin同源样域家族A成员1(PHLDA1)的m RNA和蛋白质表达结果与微阵列结果一致。STRING分析发现,PHLDA1可能与差异表达基因SLC38A3、SLC5A5、Lnc-SLC36A4-1和Lnc-PLEKHJ1-1具有物理性和/或功能性相互作用,这有望揭示VEC在缺氧/复氧环境下长链非编码RNA(lnc RNA)的相关机制。     

运动与Th1和Th2细胞平衡

为了了解Th1和Th2细胞平衡及运动对Th1和Th2细胞平衡的影响,通过文献综述的方法论述了Th1和Th2细胞的生物特征、Th1和Th2型细胞因子的生物功能,以及运动对Th1和Th2细胞平衡的影响和运动引起Th1和Th2平衡偏移的可能机理及调节方法。     

雌二醇对EAE大鼠ICAM-1和INF-γ表达的影响

目的探讨雌二醇对去卵巢后EAE大鼠的免疫抑制作用及其机制.方法制备去卵巢后10 d的EAE大鼠模型,随机分为EAE组、E2治疗组和佐剂组.E2治疗组在免疫后（post-inoculation,p.i.）0～5 d每日肌注E2（250μg/kg）一次.在p.i.6,8,10,12,14,16 d处死动物,用免疫组化技术检测脑和脊髓不同部位ICAM-1,IFN-γ的表达,并对EAE组ICAM-1和IFN-γ进行相关分析.结果与EAE组相比较,在p.i.6～12 d,E2治疗组血清E2浓度升高,临床症状评分降低,体重减轻减少,发病率较低,上述差异均有统计学意义;在p.i.10～16 d,E2治疗组ICAM-1与IFN-γ的表达降低,且具有统计学意义.EAE组ICAM-1与IFN-γ表达呈显著正相关性.结论E2能够有效抑制EAE,其抑制机制与E2下调ICAM-1,IFN-γ的表达进而促使Th细胞发生转分化有关。     

人白血病细胞系KG1a中P2X7受体的表达和功能研究

用半定量RT-PCR和流式细胞术，研究了白血病细胞系KG1a中P2X7受体在基因和蛋白水平的表达。用荧光分光光度计，测定了用激动剂三磷酸腺苷（ATP）和苯甲酰苯甲酸ATP（BzATP）刺激前后细胞内钙离子浓度的变化，证明P2X7受体的功能。结果表明：KG1a细胞表达P2X7受体，且在激动剂的刺激下能引起KG1a细胞通过P2X7受体的胞外钙内流；去除胞外钙离子时，激动剂不能引起胞内钙浓度的升高；提示KG1a细胞表达P2X7受体的基因和功能蛋白，激活该受体引起胞外钙离子的内流。     

NE对THP-1细胞IL-6 HMGB1和CD137表达的影响

目的:探讨去甲肾上腺素对人THP-1细胞CD137、IL-6及HMGB1表达的影响.方法:加入101μmol/L的去甲肾上腺素处理THP-1细胞1小时.运用逆转录多聚酶链反应检测其CD137、IL-6及HMGB1mRNA的表达.结果:10μmol/L的去甲肾上腺素能上调THP-1细胞CD137及IL-6mRNA的表达,与对照组比较差异有显著性(P<0.01);但对HMGB1mRNA的表达没有影响.结论:去甲肾上腺素上调THP-1细胞CD137及IL-6mRNA水平.     

金线莲多糖对Hep1-6细胞活性的影响

通过采用MTS法、流式细胞术法以及构建RAW264.7/Hep1-6细胞共培养模型检测经不同浓度(0、125、250、500、1 000、2 000μg/mL)金线莲多糖(Anoectochilus roxburghii Polysaccharides, APS)处理的RAW264.7细胞和Hep1-6细胞的增殖及周期情况,研究APS对Hep1-6细胞生长的影响.直接使用APS处理Hep1-6细胞,发现不同浓度APS对Hep1-6细胞增殖无抑制作用,且对其周期无影响.由共培养模型可知,不同浓度APS先作用于RAW264.7细胞24 h后,将RAW264.7细胞及其培养液与Hep1-6细胞共培养24 h后,0、125、500μg/mL试验组与不加药无RAW264.7空白对照组相比,Hep1-6细胞受到一定抑制作用,细胞毒性为1级. 125、500μg/mL试验组与不加药含RAW264.7对照组相比,Hep1-6细胞具有显著抑制(P <0.01),但2 000μg/mL试验组中Hep1-6细胞生长率高于对照组. APS直接作用于Hep1-6对其活性无影响,而RAW264.7与Hepl-6共培养后,RAW264.7可以抑制Hepl-6的生长,加APS处理RAW264.7后再与Hepl-6共培养,一定浓度范围内可以进一步抑制Hepl-6的生长,但过高浓度的APS反而对共培养的Hepl-6生长有一定促进作用.     

和声2-5-1的进行模式

和声进行中的2-5-1进行就是:二级和弦-五级和弦-一级和弦的一种特有的进行模式.这种进行模式是爵士音乐所固有的一种基础和弦进行模式,因为它是建立在一个自然大调或小调内的二级、五级和一级和弦之上的进行模式,所以我们首先需要了解的是这三个和弦在调内的固定属性.     

人白血病细胞系KG1a中P2X7受体的表达和功能研究

用半定量RT-PCR和流式细胞术,研究了白血病细胞系KG1a中P2X7受体在基因和蛋白水平的表达.用荧光分光光度计,测定了用激动剂三磷酸腺苷(ATP)和苯甲酰苯甲酸ATP(BzATP)刺激前后细胞内钙离子浓度的变化,证明P2X7受体的功能.结果表明KG1a细胞表达P2X7受体,且在激动剂的刺激下能引起KG1a细胞通过P2X7受体的胞外钙内流;去除胞外钙离子时,激动剂不能引起胞内钙浓度的升高;提示KG1a细胞表达P2X7受体的基因和功能蛋白,激活该受体引起胞外钙离子的内流.     

厌氧/缺氧/好氧-膜生物反应器对焦化废水中萘的去除研究

为了考察膜生物反应器(membrane bioreactor,MBR)对焦化废水中萘的去除特性,采用小试规模厌氧/缺氧/好氧-膜-生物反应器(A_1/A_2/O-MBR)系统处理实际焦化废水,研究了萘在A_1/A_2/O-MBR处理系统中的存在特性与去除效果。研究结果表:A_1/A_2/O-MBR系统能有效去除焦化废水中的萘,当进水的质量浓度为(265.5±119.0)μg/L时,去除率为(99.4±0.5)%,出水的平均质量浓度为(0.95±0.59)μg/L。生物处理是A_1/A_2/O-MBR系统水相中萘去除的主要途径,膜的截留对萘没有强化去除作用。     

Co1-xCrxFe2O4的制备及其析氧性能研究

利用高温固相反应法,使用Fe_3O_4、Co_2O_3和Cr_2O_3为原料,制备了Cr掺杂的Co_(1-x)Cr_xFe_2O_4(x=0.10,0.15,0.20,0.25)粉末,对不同掺杂量的样品使用X射线衍射仪和扫描电子显微镜进行结构和形貌的表征,同时对样品进行了电催化析氧活性测试.结果表明,随着Cr掺杂量的增加,样品的晶格常数逐渐减小.当掺杂量为x=0.1时,样品具有最高的电催化析氧活性,在电流密度为10 mA/cm~2时,其析氧过电位为338 m V,塔费尔斜率为47 mV/dec.     

Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration

Objective  

Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the molecular mechanisms have not been elucidated. The aim of the present study was to investigate the regulatory function of BMP-2 in the migration of COS-7 cells and the underlying mechanisms.     

STC-1000机床自动换刀故障分析与排除

五轴联动数控机床在使用过程中,换刀的故障比较频繁,占设备故障的50%以上,影响了其工作效率。通过对数控机床ST C-1000换刀故障分析与排除方法的研究,从实际应用的角度对ST C-1000换刀故障进行了原因分析,通过PLC 具体调整实例提出了排除方法的应用。     

Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma

Objective  

Mitogen-activated protein kinases (MAPKs) are correlated with a more malignant phenotype in many cancers. This study was designed to evaluate the predictive value of the expression of MAPK phosphatase-1 (MKP-1) and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK_1/2), as the key regulatory mechanism of the MAPKs, in lung squamous cell carcinoma (SCC).     

Education for citizenship in Key Stages 1 and 2

ABSTRACT

This paper considers the current context and looks ahead to the opportunities that may now exist to develop education for citizenship. The place of citizenship in Key Stages 1 and 2 is discussed in the light of the National Commission's recommendations, the Dearing review of the curriculum and OFSTED's inspection framework. Questions about what is happening in schools are then raised. Answers to these questions were pursued through case‐studies of ten schools. An account of the findings under nine headings is presented and key issues summarized.     

Inducible nitric oxide synthase expression is related to angiogenesis,bcl-2 and cell proliferation in hepatocellular carcinoma

In this study, we examined the expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) by immunohistochemical staining in 76 tissue sections collected from hepatocellular carcinoma (HCC) patients undergoing hepatectomy. Microvascular density (MVD) was determined by counting endothelial cells immunostained using anti-CD34 antibody. We performed DNA-flow cytometric analyses to elucidate the impact of iNOS and VEGF expression on the cell cycle of HCC. Most of the HCC cells that invaded stroma were markedly immunostained by iNOS antibody. The iNOS stain intensity of the liver tissue close to the tumor edge was stronger than that of HCC tissue, and the strongest was the hepatocytes closer to the tumor tissue. However, iNOS expression in 10 normal hepatic samples was undetectable. VEGF positive expression ratio was 84.8% in iNOS positive expression cases, and the ratio was 35.3% in negative cases. There was significant correlation (P=0.000) between iNOS and VEGF expression. Moreover, iNOS expression was significantly associated with bcl-2 and MVD, but without p53 expression. DNA-flow cytometric analyses showed that combined expression of iNOS and VEGF had significant impact on the cell cycle in HCC. PI (Proliferating Index) and SPF (S-phase fraction) in the combined positive expression of iNOS and VEGF group was significantly higher than that in the combined negative group. The present findings suggested that iNOS expression was significantly associated with angiogenesis, bcl-2 and cell proliferation of HCC. Project supported in part by the National Ninth-Five-Years Project Fund (No. 96909121), China     

Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma

ＩＮＴＲＯＤＵＣＴＩＯＮＮｉｔｒｉｃｏｘｉｄｅ (ＮＯ) ,ａｓｈｏｒｔｈａｌｆ ｌｉｆｅｒａｄｉｃａｌ,ｉｓｈｉｇｈｌｙｒｅａｃｔｉｖｅ ,ａｎｄｉｓｉｎｖｏｌｖｅｄｉｎｍａｎｙｂｉｏ ｌｏｇｉｃａｌｐｒｏｃｅｓｓｅｓ,Ｉｔｓｅｅｍｓｔｏｐｌａｙａｎｉｍｐｏｒｔａ     

三氟甲基苯硫醚添加剂对高电压钴酸锂/石墨电池性能的影响

采用三氟甲基苯硫醚作为电解液成膜添加剂,研究添加剂对高电压钴酸锂/石墨电池性能的影响。充放电测试结果表明,1%的三氟甲基苯硫醚显著提高了高电压钴酸锂/石墨电池的循环稳定性。电池在1C倍率下经过100圈循环后的容量保持率从75.4%提高至86.9%。分别研究添加剂对钴酸锂和石墨电极循环前后表面形貌和组份的影响,结果表明三氟甲基苯硫醚能同时在钴酸锂正极和石墨负极表面形成保护膜,有效抑制电解液的分解,提高电极材料结构稳定性,从而提高钴酸锂/石墨电池的循环稳定性。     

Strategies for professional reading in L1 and L2

Reading strategies of future professionals, both in native language (L1) and in one or more foreign languages (L2), have been little explored. Findings from research concerning the transfer of L1 reading strategies to the L2 context are contradictory. Our hypothesis is that relative performance in L1 and L2 reading is associated with two key variables involved in successful L2 reading comprehension: L1 reading proficiency and L2 language competency, the latter being the stronger predictor. 39 students were divided into three groups by strength or weakness on each independent variable. Their reading strategies, explored by questionnaire, were compared within and between groups, both in L1 and L2, and were correlated to text recall comprehension scores. Readers strong on both key variables were equally effective in both languages. Strong L1 readers weak in L2 were poor L2 readers but fared better than readers weak on both variables. General reading strategies differentiated efficient readers from poor ones both in L1 and L2, but specific problem-solving strategies were the main obstacle in L2. Comprehending – the process, or strategic approach employed – correlated very strongly with comprehension – the product, or score – in L2 and fairly strongly in L1. Pedagogical implications suggest individualised reading training in the light of relative difficulties.     

母语对二语语义习得的影响探究

如何看待母语在二语语义习得过程中的作用是二语习得研究不可避免的问题。本文在国内外相关母语迁移理论和研究的基础上,总结了母语对二语语义习得的影响,提出母语在二语语义习得问题上的辩证性作用。