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1.
Rashmi Raghuvanshi M. Chandra P. C. Misra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2005,20(1):26-29
Platelets play important role in precipitating ischaemic myocardial syndromes in many ways. One of the consequences of ischaemic
diseases is excessive generation of oxygen derived free radicals that have numerous pathophysiological consequences. Platelet
pro-oxidant enzyme, xanthine oxidase is one of the sources of generation of free radicals. In the present paper, we report
the effect of administration of vitamin E along with aspirin on the levels of platelet xanthine oxidase and extent of free
radical mediated damage in the patients reperfused after myocardial infarction.
Our findings show that administration of 400 mg. vitamin E for six days along with 80 mg. aspirin has an excellent anti-oxidant
effect as evidenced by reduced platelet xanthine oxidase activity and lowering of malondialdehdye (MDA) levels which is an
index of the extent of free radical mediated damage. 相似文献
2.
G. M. Rao P Sumita M Roshni M. N. Ashtagimatt 《Indian journal of clinical biochemistry : IJCB》2005,20(1):198-200
It has been suggested that antioxidant systems are impaired in pregnancy induced hypertension and hence patients are exposed
to oxidative stress. In order to investigate the relationship between lipid peroxidation and certain antioxidant parameters
in blood of pregnancy induced hypertension (PIH) cases, 25 normotensive and 23 PIH samples were studied. In the present study,
thiobarbituric acid reactive substances showed a tendency to increase, however the increase remained statistically insignificant.
Plasma ascorbic acid level remained unaltered and Vitamin E showed a tendency to increase in the study group. The findings
implicate oxidative stress in the disease and cite the biochemical rationale for clinical trials of antioxidants to prevent
and treat pregnancy induced hypertension. 相似文献
3.
Vivek K. Dwivedi Mahesh Chandra P. C. Misra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2005,20(1):21-25
Effect of administration of 600 mg. vitamin E each day, for six days, was observed on activity of some of the anti-oxidant
enzymes and levels of malondialdehyde (as an index of free radical mediated damage) in the platelets of patients reperfused
after myocardial infarction. It has been found that vitamin E administration significantly lowers the level of malondialdehyde
in the patients. Vitamin E administration increases the activities of anti oxidant enzymes (viz. superoxide dismutase, glutathione
reductase and catalase) tested both in the patients and healthy controls. Vitamin E administration causes general stimulation
of anti-oxidant enzyme activities both in healthy persons and the patients, however, lowering of lipid per-oxidation upon
administration of vitamin E is specific for patients. These findings exhibit beneficial role of vitamin E administration in
the management of the patients reperfused after myocardial infarction. 相似文献
4.
Sarita N. Chavan Umesh More Shruti Mulgund Vishal Saxena Alka N. Sontakke 《Indian journal of clinical biochemistry : IJCB》2007,22(2):101-105
Osteoporosis encompasses a wide spectrum of conditions associated with imbalance of osteoclastic and osteoblastic activities.
The increased activity of osteoclasts leads to increased free radical formation and hence lipid peroxidation. Present study
probes into the role of antioxidants as a palliative treatment for osteoporosis. It involved 50 healthy controls and 75 clinically
diagnosed osteoporosis patients. Both the groups underwent baseline assessment of biochemical markers viz. osteoblastic markers:
serum Alkaline phosphatase. Free or ionic calcium and Inorganic phosphorus, osteoclastic markers: serum Tartarate resistant
acid phosphatase and Malondialdehyde and the antioxidant status: serum Superoxide dismutase and Erythrocyte reduced glutathione.
The osteoporotic group was then divided into groups A (Vitamin E-Evinal 400 mg), B (Vitamin C-Celin 500 mg), C (Vitamin E+C-Evinal+Celin)
for antioxidant supplementation for a period of 90 days. The results reveal that there is significant fall in concentration
of serum MDA (p<0.001), TrACP (p<0.01). Improvement in antioxidant status is reflected by significant rise in concentration
of serum SOD (p<0.001) and erythrocyte GSH (p<0.001) after 90 days of antioxidant supplementation in osteoporosis. The findings
indicate that on the whole bone status improved with prolonged antioxidant vitamin supplementation, which can be used as a
palliative treatment for osteoporosis. The efficacy is not affected whether the vitamins are administered singly or conjointly. 相似文献
5.
Hem Lata G. K. Ahuja A. P. S. Narang Lily Walia 《Indian journal of clinical biochemistry : IJCB》2004,19(2):1-4
Stress is one of the basic factors in the aetiology of a number of diseases such as atherosclerosis, coronary heart disease,
aging, liver disease etc. Hence this work was designed to study the effect of stress in the form of forced immobilisation
stress on lipid peroxidation and lipid profile in rabbits. The study was conducted in 25 healthy rabbits of either sex. Rabbits
were subjected to forced immobilisation for two hours everyday for consecutive seven days. Blood samples were collected on
day 1, 3 and 7 after immobilisation period. Malondialdehyde (MDA), a marker of lipid peroxidation was estimated by TBA assay
method. Lipid profile was analysed by multichannel autoanalyser. There was statistically significant increase in MDA, total
cholesterol, LDL, VLDL and triglycerides with immobilisation. These findings indicate that stress in the form of forced immobilisation
increases lipid peroxidation and alter lipid profile, which may be responsible for pathophysiology of various diseases. 相似文献
6.
Prasannachandra Vivian D'Souza Benedicta D'Souza 《Indian journal of clinical biochemistry : IJCB》2006,21(2):103-106
Reactive oxygen species are thought to be involved in the pathogenesis of Malaria. To assess the extent of oxidative stress,
a study was conducted in patients withPlasmodium falciparum malaria andPlasmodium Vivax malaria. Plasma Thiobarbituric acid reactive substances (TBARS) were measured to assess the degree of lipid peroxidation.
Antioxidant status was measured by estimating the levels of Vitamins E and C. Results were compared with age and sex matched
control subjects. This study suggests that plasma TBARS levels were significantly increased in malaria patients. The patients
withP. falciparum infection showed significantly increased levels of lipid peroxides when compared toP. vivax malaria. The antioxidant Vitamins E and C were decreased significantly in malaria patients in both the groups. Maximum decline
in Vitamin C was observed inP. vivax malaria. Therefore it is been hypothesized that antioxidant Vitamins E and C could provide protection against the oxidative
stress induced by malaria. 相似文献
7.
R. B. Bhogade A. N. Suryakar N. G. Joshi R. Y. Patil 《Indian journal of clinical biochemistry : IJCB》2008,23(3):233-237
Hemodialysis represents a chronic stress status for its recipients. Many hypotheses state that this may be associated with
oxidative stress. Thus, there may be deficiency of antioxidants like erythrocytic superoxide dismutase, catalase, vitamin
E or increased generation of free radicals like superoxide anions. A study was carried out to investigate oxidant and antioxidant
status in chronic renal failure patients undergoing hemodialysis and effect of vitamin E supplementation on these two status.
Blood samples were collected from patients before and after hemodialysis and from controls. The samples were analyzed for
quantitation of MDA as index of lipid peroxide, nitric oxide, vitamin E, vitamin C and enzymatic antioxidants namely erythrocyte
SOD and catalase. As compared to controls, the levels of serum MDA were significantly increased and activities of erythrocyte
SOD and catalase, levels of serum nitric oxide, serum vitamin E and plasma vitamin C were significantly decreased both before
and after hemodialysis. The efficiency of vitamin E therapy in hemodialysis patients was assessed by re-evaluating oxidant
and antioxidant status of same patients after supplementation of vitamin E. Vitamin E supplementation caused decrease in serum
MDA and increase in levels of serum nitric oxide, vitamin E, vitamin C and activities of erythrocytic SOD and catalase. Our
results suggest the presence of oxidative stress and the possible preventive role of vitamin E therapy in hemodialysis patients. 相似文献
8.
The objective of the present study is to evaluate the beneficial effect of tomatoes, which are rich source of Lycopene, relatively
a new carotenoid known to play an important role in human health and disease. In this study lipid peroxidation rate was measured
by estimating Malondialdehyde and the levels of serum enzymes involved in antioxidant activities like Super Oxide Dismutase,
Glutathione Peroxidase, Glutathione Reductase, Reduced Glutathione, in type-II diabetic group (n=40) and age matched control
group (n=50), and observed significantly lower levels of antioxidant enzymes and very high lipid peroxidation rate in type-II
diabetes when compared to control group (p<0.001). Short term supplementation with tomatoes (cooked) to diabetic group for
a period of 30 days, showed a significant improvement in antioxidant enzyme levels (p<0.001) and decreased lipid peroxidation
rate (p<0.001) suggesting the supplementation with tomato lycopene may serve as the best method of preventing the oxidative
stress in diabetic patients. 相似文献
9.
Mukesh Nandave Ipseeta Mohanty T. C. Nag Shreesh Kumar Ojha Rajan Mittal Santosh Kumari Dharamvir Singh Arya 《Indian journal of clinical biochemistry : IJCB》2007,22(1):22-28
The present study evaluated the cardioprotective potential of vitamin-E by studying its effect on hemodynamic parameters,
lipid peroxidation, myocyte injury marker and ultrastructural changes in model of isoproterenol-induced myocardial necrosis
in rats. Wistar albino male rats (150–200 g) were randomly divided into saline, ISP control, and vit E groups. Vitamin E group
was administered vitamin E at a dose of 100mg/kg/day while saline and ISP control groups received saline orally for one month.
On 29th and 30th day, ISP (85 mg/kg, sc) was administered at an interval of 24 h to vit E and ISP control rats. On 31st day, rats of all groups were anesthetized and hemodynamic parameters were recorded. At the end of experimentation, animals
were sacrificed; hearts were excised and processed for biochemical and ultrastructural studies. ISP administration produced
marked cardiac necrosis as evidenced by significant decrease in my ocardial creatine kinase-MB as well as increase in malonaldialdehyde
levels. ISP-induced myocardial necrosis resulted in myocardial dysfunction as evidenced by significant depression in heart
rate and mean arterial pressure in the ISP control group as compared to saline control. Salient ultrastructural changes including
extensive loss of myofibrils, muscle necrosis, loss of mitochondria, and formation of several intracytoplasmic vacuoles and
lipid droplets further confirmed the ISP-induced myocardial damage. However, subsequent to ISP challenge, vit E treatment
significantly preserved the myocardium by restoring myocardial CK-MB activity, inhibiting the ISP-induced lipid peroxidation
and ultrastructural changes. Additionally, pre-and co-treatment of vit E prevented the deleterious ultrastructural changes
caused by ISP. These beneficial effects of chronic vit E treatment also translated into significant restoration of the altered
hemodynamic parameters. The present study clearly demonstrated the cardioprotective potential of vit E at dose of 100 mg/kg
in ISP-induced model of myocardial necrosis in rats. The significant restoration of altered hemodynamic parameters, myocardial
CK-MB activity, prevention of ISP-induced rise in lipid peroxidation and ultrastructural changes may confirm its cardioprotective
effect. 相似文献
10.
Eshrat M. Halim A. K. Mukhopadhyay 《Indian journal of clinical biochemistry : IJCB》2006,21(2):181-188
This study was carried out to see the effect of the aqueous extract ofOcitum sanctum Linn (Tulsi) with Vitamin E on biochemical parameters and retinopathy in the streptozotocin-induced diabetic albino male
rats. Adult albino male rats weighing 150–200 gm were made diabetic by intraperitoneal injection of streptozotocin in the
dose 60 mg/kg in citrate buffer (pH 6.3). The diabetic animals were left for one month to develop retinopathy. Biochemical
parameters like plasma glucose, oral glucose tolerance and glycosylated hemoglobin HbA1c, were measured along with lipid profile, and enzymes like glutathione peroxidase (GPX), lipid peroxidase (LPO), superoxide
dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) in normal, untreated diabetic rats and diabetic rats treated
withOcimum sanctum L extracts and vitamin E. Fluorescein angiography test was done for assessing retinopathy. Results on biochemical parameters
were analyzed statistically by using ANOVA followed by Dunnet's ‘t’-test. A p-value of <0.05 was considered as significant.
Evaluation of biochemical profile in treated groups showed statistically significant reduction in plasma levels of glucose,
HbA1c, lipid profile and LPO, and elevation of GPX, SOD, CAT and GST. Treatment of the diabetic animals withOcimum sanctum and Vitamin E, alone and in combination for 16 weeks showed reversal of most of the parameters studied including plasma glucose
levels. Angiography showed improvement in retinal changes following combined antidiabetic treatment. 相似文献
11.
Serum lipids and lipoproteins were estimated in 29 patients with acute myocardial infarction during acute phase (day 1,2,3),
predischarge and after three months. Serum total lipids, total cholesterol (TC) and LDL-cholesterol (LDLc) showed no significant
change during the hospital stay and three months followup. HDL-cholesterol (HDLc), however, started falling from day 2 onwards
with statistically significant reduction at pre-discharge and remained so at 3 months. The ratios of TC/HDLc and LDLc/HDLc
showed significant increase on predischarge day as compared to day 1. Serum triglycerides also showed an increasing trend
after myocardial infarction with a significant increase on day 3 and predischarge as compared to day 1. it is concluded that
the optimum time for assessment of serum lipid profile in patients with myocardial infarction seems to be within 24 hours
of the acute episode. 相似文献
12.
Akila V. Prashant H. Harishchandra Vivian D'souza Benedicta D'souza 《Indian journal of clinical biochemistry : IJCB》2007,22(1):131-134
Advanced age is associated with an accumulation of free radical damage, which leads to physiological and clinical modifications.
Age related changes resulting from free radical reactions include increasing levels of lipid peroxides, alterations in enzyme
activities and greater osmotic fragility. The present study was conducted to estimate the level of lipid peroxidation product-Malondialdehyde
and antioxidants Catalase and Glutathione in elderly people. An increase in lipid peroxidation and decrease in antioxidants
was observed in normal elderly people. Highly significant increase in MDA and decrease in antioxidants was observed in elderly
people when complicated with diabetes and hypertension. Supplementation of antioxidants may prevent further oxidative injury
in elderly people. 相似文献
13.
Vaishali N. Thorat Adinath N. Suryakar Pradeep Naik Bipin M. Tiwale 《Indian journal of clinical biochemistry : IJCB》2009,24(1):102-104
Liver transplantation is an accepted therapy for chronic liver disease patients. These patients generally have low levels
of fat soluble vitamins, which have important antioxidant roles. Therefore, this study was undertaken to investigate whether
such patients had evidence of antioxidant depletion and increased lipid peroxidation before transplant and whether the subsequent
ischemia and reperfusion encountered during liver transplantation have any effect on antioxidant levels and lipid peroxidation.
We assessed plasma total antioxidant capacity and serum lipid peroxide in 12 patients undergoing liver transplantation and
equal numbers of healthy subjects. We found that before reperfusion, antioxidant levels were significantly decreased along
with significantly elevated lipid peroxidation levels as compared with healthy controls (P<0.001). On reperfusion of the liver
graft, further declined values of total antioxidant accompanied with highly elevated lipid peroxidation were seen than those
of pre-reperfusion samples (P<0.001). This data shows that patients undergoing liver transplant have lowered antioxidant defenses
and evidence of free radical damage, which compound the additional insult of reperfusion injury. Therefore antioxidant therapy
in these patients before transplantation may ameliorate the effects of reperfusion. 相似文献
14.
Uma S. Bhartiya Yogita S. Raut Lebana J. Joseph Rohini W. Hawaldar Badanidiyoor S. Rao 《Indian journal of clinical biochemistry : IJCB》2008,23(4):382-386
The aim of this study was to evaluate the radioprotective effect of turmeric extract (40 mg/kg body weight) and vitamin E
(α- tocopherol acetate, 400 IU/kg body weight) supplementation on lipid peroxidation, reduced glutathione and antioxidant
defense enzymes in various organs like liver, kidney and salivary glands at 24 h in adult Swiss mice. 131Iodine exposure significantly increased lipid peroxidation in kidney and salivary glands in comparison to control animals.
Pre supplementation with turmeric extract for 15 days showed significant lowering of lipid peroxidation in kidney. On the
other hand vitamin E pre supplementation showed marked reduction in lipid peroxidation in salivary glands. Reduced glutathione
levels decreased significantly in liver after radiation exposure. However, pre supplementation with turmeric extract and vitamin
E did not improve glutathione levels in liver. In conclusion, we have observed differential radioprotective effect of turmeric
extract and vitamin E in kidney and salivary glands. However, Vitamin E seems to offer better radioprotection for salivary
glands which is known to be the major site of cellular destruction after radioiodine therapy in patients. 相似文献
15.
Hilda Priya D'Souza H. Ramachandra Prabhu 《Indian journal of clinical biochemistry : IJCB》2006,21(2):138-141
The beneficial effects of ∈-3 fatty acids on human health have been well documented. Fish and fish oils are the richest sources
of ∈-3 fatty acids. However, due to their high degree of unsaturation, they are highly susceptible to lipid peroxidation.
Regular consumption of peroxidised oils may represent a risk factor for the induction and development of atherosclerosis.
In view of the above reports, it was considered necessary to study the effects of tumeric on fish lipid peroxidation during
standard cooking practices and on time-dependent changes in the peroxidation of fish homogenate. The antioxidant effect of
α-tocopherol was also studied to confirm the relevance of the study. The results suggest that turmeric may be considered as
a safe, cheap and readily usable antioxidant for food preparations. 相似文献
16.
J. O. Olanlokun 《Indian journal of clinical biochemistry : IJCB》2008,23(1):62-66
The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels,
antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated
lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that
oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the
diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while
the level of lipid peroxidation decreased. 相似文献
17.
Sadanand B. Patil M. V. Kodliwadmath Sheela M. Kodliwadmath 《Indian journal of clinical biochemistry : IJCB》2008,23(1):45-48
The present study was designed to evaluate the lipid peroxidation and non-enzymatic antioxidants in pregnancyinduced hypertension
(PIH) compared with healthy normal pregnant and non-pregnant as controls. 75 cases were studied of which 25 were normal healthy
controls (Group A), 25 healthy pregnant women (Group B) of third trimester and 25 were PIH (Group C) of the same trimester.
In PIH, malondialdehyde a lipid peroxidation product was significantly increased as compared to normal pregnant and non-pregnant
controls. The nonenzymatic antioxidants like reduced glutathione, Vitamin-E, Vitamin-C and Vitamin-A were significantly decreased
in normal pregnants as compared to controls. A further significant decrease was noticed in PIH compared to normal pregnants.
A significant negative correlation was detected between lipid peroxidation and non-enzymatic antioxidant levels. Our study
clearly indicates a relationship between elevated lipid peroxidation, decreased non-enzymatic antioxidant in PIH. The measurement
of non-enzymatic antioxidants in plasma may be useful predictor of the likely development of PIH. 相似文献
18.
Aiki Kaul Rishi Sethi Mithilesh K. Misra 《Indian journal of clinical biochemistry : IJCB》2009,24(1):49-51
A comparative study on the levels of erythrocyte adenosine deaminase and lipid peroxidation has been undertaken in post myocardial
infarction angina patients along with age and sex matched healthy individuals serving as control. Present findings show that
levels of adenosine deaminase is highly elevated in post myocardial infarction angina patients compared to healthy persons.
Malondialdehyde levels are also significantly increased in post myocardial infarction angina patients. The study shows that
adenosine deaminase has an important implication in ischemic myocardial syndrome. 相似文献
19.
Arun Garg Pankaj Abrol AD Tewari Rajiv Sen Harbans Lal 《Indian journal of clinical biochemistry : IJCB》2005,20(1):85-86
Fifty children (1–4 years age) presenting with microcytic hypochromic anemia (hemoglobin less than 10g/dl) were studied in
two groups of 25 each. Group I was supplemented with iron (ferrous sulphate 6 mg/kg/d) while group II in addition to iron
was also supplemented with vitamin A (5000 IU/d). Hemoglobin concentration was found to be significantly increased after 4
weeks of iron supplementation. Rise in hemoglobin was comparatively more in-group II, as compared to group I, after 8 and
12 weeks. Serum iron was significantly higher after 4 weeks in both the groups. Packed cell volume (PCV) and retinol levels
increased significantly in-group II only. The data suggests that supplementation of vitamin A improves hematopoiesis. 相似文献
20.
Anjali C. Warrier Narasimha Y. Rao Devdutta S. Kulpati Tarun K. Mishra Bhaskar C. Kabi 《Indian journal of clinical biochemistry : IJCB》1995,10(1):9-13
There was increase in erythrocyte lipid peroxidation and adenosine deaminase (ADA) activity in red blood cells (RBC's), white
blood cells (WBC's) and plasma, in 30 patients of confirmed diabetes mellitus, 10 each of insulin dependent diabetes mellitus
(IDDM or type I), non-insulin dependent diabetes mellitus (NIDDM or type II) and diabetes with ketoacidosis when compared
with 20 healthy individuals (controls). Glycosylated hemoglobin A1c%, plasma free fatty acid (FFA) and glycerol levels were
also elevated in all the cases when compared with controls. The increase was more in diabetic ketoacidosis group. Significant
positive correlations were seen between erythrocyte lipid peroxidation and ADA activities in RBC's, WBC's and plasma. It is
suggested that decreased tissue adenosine levels due to increase in ADA activity, is related to the severity of hyperglycemia
and lipid peroxidation in diabetes mellitus. 相似文献