Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii

INTRODUCTION Methyl transfers are alkylation reactions central to cellular biochemistry, by far, S-adenosyl-L-me-thionine (AdoMet) is the most commonly used methyl donor. AdoMet-dependent methyltransferases (MTases) involved in biosynthesis, detoxification, signal transduction, protein sorting and repair, chromatin regulation and gene silencing (Armengaud et al., 2004; Schubert et al., 2003). Including DNA MTase, RNA MTase, protein MTase, phospholipids and small molecule MTase, …