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1.
Objective To investigate the protective effect of nicorandil and amlodipine in isoproterenol induced myocardial necrosis in rats.
Methods The effect of nicorandil and amlodipine on bio-chemical parameters during isoproterenol induced myocardial necrosis in rats
was examined by determining the activity of creatine phosphokinase (CK), lactate dehydrogenase (LDH), and transaminases (AST,
ALT) in the serum of the animals.
Results Isoproterenol (150mg/kg/day) administered rats showed statistically significant rise in activities of LDH(1.02±0.19 to 1.85±0.05),
CK(4.3±0.19 to 7.37±.27), AST(0.38±.03 to 0.78±.05) and ALT(0.19±.017 to 0.346±.027) in the serum. Pre-co-treatment of rats
with nicorandil and amlodipine significantly lowered the raised levels of these enzymes and thereby restoring the enzyme activity
to near normal as was clear from the chart i.e. LDH was 1.10±0.04, CK was 4.37±0.19, AST and ALT were 0.39±0.028 and 0.199±0.04
respectively.
Conclusion It is demonstrated that pre-co-treatment with nicorandil and amlodipine either alone or in combination help in providing protective
effect on isoproterenol induced myocardial necrosis. 相似文献
2.
Enzymology is a diagnostic indicator for myocardial infarction and diabetes in hypertension patients. Therefore the selection
of methods for measurement of cardiac enzyme, Aspartate transaminase (AST), Creatine kinase(CK), and isoenzyme of creatine
kinase (MB form), determine the effectiveness of antihypertension drug would provide the physician with diagnostic and prognostic
clinical evidence. 相似文献
3.
Mukesh Nandave Ipseeta Mohanty T. C. Nag Shreesh Kumar Ojha Rajan Mittal Santosh Kumari Dharamvir Singh Arya 《Indian journal of clinical biochemistry : IJCB》2007,22(1):22-28
The present study evaluated the cardioprotective potential of vitamin-E by studying its effect on hemodynamic parameters,
lipid peroxidation, myocyte injury marker and ultrastructural changes in model of isoproterenol-induced myocardial necrosis
in rats. Wistar albino male rats (150–200 g) were randomly divided into saline, ISP control, and vit E groups. Vitamin E group
was administered vitamin E at a dose of 100mg/kg/day while saline and ISP control groups received saline orally for one month.
On 29th and 30th day, ISP (85 mg/kg, sc) was administered at an interval of 24 h to vit E and ISP control rats. On 31st day, rats of all groups were anesthetized and hemodynamic parameters were recorded. At the end of experimentation, animals
were sacrificed; hearts were excised and processed for biochemical and ultrastructural studies. ISP administration produced
marked cardiac necrosis as evidenced by significant decrease in my ocardial creatine kinase-MB as well as increase in malonaldialdehyde
levels. ISP-induced myocardial necrosis resulted in myocardial dysfunction as evidenced by significant depression in heart
rate and mean arterial pressure in the ISP control group as compared to saline control. Salient ultrastructural changes including
extensive loss of myofibrils, muscle necrosis, loss of mitochondria, and formation of several intracytoplasmic vacuoles and
lipid droplets further confirmed the ISP-induced myocardial damage. However, subsequent to ISP challenge, vit E treatment
significantly preserved the myocardium by restoring myocardial CK-MB activity, inhibiting the ISP-induced lipid peroxidation
and ultrastructural changes. Additionally, pre-and co-treatment of vit E prevented the deleterious ultrastructural changes
caused by ISP. These beneficial effects of chronic vit E treatment also translated into significant restoration of the altered
hemodynamic parameters. The present study clearly demonstrated the cardioprotective potential of vit E at dose of 100 mg/kg
in ISP-induced model of myocardial necrosis in rats. The significant restoration of altered hemodynamic parameters, myocardial
CK-MB activity, prevention of ISP-induced rise in lipid peroxidation and ultrastructural changes may confirm its cardioprotective
effect. 相似文献
4.
A serial follow up study of cardiac marker enzymes during the week after acute myocardial infarction
Laboratory infarction diagnostics are based on the detection of elevated serum activities of creatine kinase (CK) Creatine
kinase Isoenzyme MB (CKMB) and Transaminases. Determination of these cardiac marker enzymes permits the diagnosis of transmural
myocardial infarction. However in such patients the diagnosis of acute myocardial infarction can be confirmed by the clinical
symptoms and changes in the ECG, in addition to the enzyme assays. The 50 AMI patients selected in the present study were
those admitted to the ICCU of Shri Krishna Hospital, Karamsad. The blood samples were taken at Zero hours (i.e. at the time
of admission of the patient). Within 6 hrs of the starting of chest pain, 1.5 million units of streptokinase were mixed with
100 to 150ml of normal saline and administered by infusion over a period of one hour. The blood samples were further collected
at intervals of 6 hrs, 14hrs, 32hrs, 48hrs, 5th day and 7th day. The blood samples were analyzed for CK, CKMB, SGOT, α HBDH and Cardiac specific Troponin T. By 6hrs the CK and CKMB
values had started rising, the rise continuing at 14hrs with peak values at 32hrs. The CK showed a slight decrease by 48 hrs.
The cardiac Troponin T showed wide time window from 4 hrs to 7th day for detecting myocardial damage. The maximum cardiac Troponin T values were during the first 24hrs. Cardiac Troponin
T in serum appears to be a more sensitive and early indicator of myocardial cell injury in comparison to CKMB. 相似文献
5.
Alka Singh Sheila Uthappa V. Govindaraju T. Venkatesh 《Indian journal of clinical biochemistry : IJCB》2002,17(1):25-28
The present study conducted on twenty-five uncomplicated cases of acute myocardial infarction diagnosed by clinical and electrocardiographic
findings indicated significantly increased level of cardiac Troponin-T and increased activities of the enzymes total creatine
kinase, creatine kinase-MB, aspartate transaminase and lactate dehydrogenase as compared to the twenty-five healthy control
subjects. The level of cardiac Troponin-T and the activities of the enzymes total creatine kinase, creatine kinase-MB, aspartate
transaminase and lactate dehydrogenase was found to be higher in “Q” wave myocardial infarction patients as compared to the
non-“Q” wave and the controls. Since cardiac Troponin-T has been shown to increase in unstable angina and renal failure without
cardiac disease and creatine kinase-MB activity has been found to be normal in patients with unstable angina and increase
very slightly in patients with renal failure, it was concluded that a combination of cardiac Troponin-T and creatine kinase-MB
activity was sufficient for the early diagnosis of acute myocardial infarction. 相似文献
6.
The present study was carried out to evaluate the antidiabetic effect of T. arjuna stembark extract and to study the activities
of hexokinase, aldolase and phosphoglucoisomerase, and gluconeogenic enzymes such as glucose-6-phosphatase and fructose-1,6-diphosphatase
in liver and kidney of normal and alloxan induced diabetic rats. Oral administration of ethanolic extract of bark (250 and
500mg/kg body weight) for 30 days, resulted in significant decrease of blood glucose from 302.67±22.35 to 82.50±04.72 and
in a decrease in the activities of glucose-6-phosphatase, fructose-1,6-disphosphatase, aldolase and an increase in the activity
of phosphoglucoisomerase and hexokinase in tissues. However, in the case of 250 mg/kg body weight of extract, less activity
was observed. The study clearly shows that the bark extract ofT. arjuna possesses potent antidiabetic activity. 相似文献
7.
Rimi Shukla Kiran Anand K. M. Prabhu P. Suryanarayana Murthy 《Indian journal of clinical biochemistry : IJCB》1995,10(1):14-18
The hypocholesterolemic effect of the water extract of the bark ofFicus bengalensis was investigated in 3 groups of rabbits, 5 in each group. Group 1 rabbits served as healthy controls and were fed with groundnut oil 1 ml/kg body wt. (bw) for five weeks. Groups 2 and 3 were made hypercholesterolemic by feeding orally cholesterol suspended in groundnut oil (1 ml/kg bw) at a dose of 100 mg/kg bw/day. Group 2 animals (untreated) continued to get the same amount of cholesterol for another four weeks. Group 3 animals received water extract of the bark (50 mg/kg bw/day) in addition to cholesterol as above. At the end of the 5th week, water extract not only prevented the elevation of serum cholesterol in the treated animals (Group 3) but also brought down its level to 160±14 mg% as compared to untreated animals (Group 2) 290±42 mg%. There was improvement in other parameters of lipid profile namely HDL & LDL+VLDL cholesterol and triacylglycerol. 相似文献
8.
Itemobong S. Ekaidem Monday I. Akpanabiatu Friday E. Uboh Offiong U. Eka 《Indian journal of clinical biochemistry : IJCB》2007,22(2):36-40
Folic acid and vitamin B12 are very important vitamins needed for normal cellular metabolic activities. The effects of folic acid and vitamin B12 on liver integrity of growing Wistar albino rats following therapeutic dose of phenytoin administration were investigated.
The activities of serum AST, ALT, ALP were investigated. Serum total protein level and lipid profile were also measured as
indices of biochemical changes. The ingestion of phenytoin alone in rats significantly reduced serum protein while AST, ALT
activities incresed as compared to the control (P<0.05). Supplementation of phenytoin with oral administration of 70microgram/kg
body wt of folic acid resulted in a significant reversal in serum total protein and suppression in serum AST and ALT activities.
Vitamin B12 supplementation did not afford any significant protection against the effect of phenytoin ingestion but rather phenytoin
toxicity was exacerbated in this study. However, the combined effects of vitamin B12 and folic acid ameliorated the effects of phenytoin on serum enzymes of experimental rats. The effect of combination of phenytoin
with folic acid or folic acid and vitamin B12 is an interesting finding. Supplementation of phenytoin with folic acid or combination of these vitamins may be recommended
for the purpose of ameliorating the adverse biochemical changes which are associated with phenytoin therapy. Further work
is ongoing to help elucidate the effects of phenytoin and these vitamins on oxidative stress inducing mechanism. 相似文献
9.
Javad Mohiti Mostafa Behjati Mohammad H. Soltani Ali Babaei 《Indian journal of clinical biochemistry : IJCB》2004,19(1):113-117
Measurement of cardiac markers is an index of care standard in the assessment and diagnosis of cardiovascualr disease. Two
of the major cardiac markers are Creatine Kinase isoenzyme CK-MB and Troponin T, which are extensively used in the diagnosis
of heart disease. The release of Troponin T and creatine kinase isoenzyme (CK-MB) was investigated in 50 coronary artery bypass
surgery patients. Measurement of plasma samples was carried out at five different time points, namely before surgery, 1,6,12,24
hours after surgery. The results indicated that CK-MB level were increased by a factor more than four times compared with
the upper limit of baseline (befor surgery). Troponin T concentration showed more than six fold over the upper limit of baseline
(before surgert) at 1,6,12,24 hours after surgery. In order to assess the significance of the length of the surgical procedure
on the release of Troponin T and CK-MB, the surgery patient were divided into two groups according to the length of the surgical
procedure: group I was selected on the basis that the surgical procedure they underwent lasted above 90 minutes and group
II with a surgical procedure below 90 minutes. Both Troponin T and CK-MB showed a significant increase in-group I compared
to group II. To investigate the likelihood that this effect is party due to myocardial infarction during surgery, the patients
were divided into two groups: Group A with some sings of myocardial infarction on Q wave of ECG and group B without any change.
The results showed approximately a two-fold increase of these markers in-group A compared to group B. Since these markers
reach into blood following damage to myocardial their increase in patients with time course surgery of more than 90 minutes
and those with a probability of MI during operation, indicating that these patient fall into a high risk group of repeat (MI)
after surgery. 相似文献
10.
Uma S. Bhartiya Yogita S. Raut Lebana J. Joseph Rohini W. Hawaldar Badanidiyoor S. Rao 《Indian journal of clinical biochemistry : IJCB》2008,23(4):382-386
The aim of this study was to evaluate the radioprotective effect of turmeric extract (40 mg/kg body weight) and vitamin E
(α- tocopherol acetate, 400 IU/kg body weight) supplementation on lipid peroxidation, reduced glutathione and antioxidant
defense enzymes in various organs like liver, kidney and salivary glands at 24 h in adult Swiss mice. 131Iodine exposure significantly increased lipid peroxidation in kidney and salivary glands in comparison to control animals.
Pre supplementation with turmeric extract for 15 days showed significant lowering of lipid peroxidation in kidney. On the
other hand vitamin E pre supplementation showed marked reduction in lipid peroxidation in salivary glands. Reduced glutathione
levels decreased significantly in liver after radiation exposure. However, pre supplementation with turmeric extract and vitamin
E did not improve glutathione levels in liver. In conclusion, we have observed differential radioprotective effect of turmeric
extract and vitamin E in kidney and salivary glands. However, Vitamin E seems to offer better radioprotection for salivary
glands which is known to be the major site of cellular destruction after radioiodine therapy in patients. 相似文献
11.
M. H. Meshkibaf A. Ebrahimi R. Ghodsi A. Ahmadi 《Indian journal of clinical biochemistry : IJCB》2006,21(1):161-164
A number of newly developed antiepileptic drugs are currently in use, among them Lamotrigine (LTG) is more common. Despite
the extensive use of this drug, it has not been possible to predict the side effects especially the hepatotoxic reactions
after long-term treatment. The present study was designed to find out alterations in the activities of liver enzymes after
chronic exposure of rats to different dose of LTG. Adults male (Wistar) rats were treated orally with LTG [5 mg/kg body weight
or 25 mg/kg body wt.] for 60 days. After the experimental period, auto analyzer carried out liver function tests. The liver
histopathology was obtained after scarifying the rats. There was a significant increase in the level of ALP, AST, ALT and
bilirubin at therapeutic dose of LTG. The increase level of these enzymes and bilirubin at toxic dose were much higher and
significant. However, the total protein and albumin significantly decreased at toxic dose of LTG. Elevation of liver enzymes
and bilirubin after chronic exposure of rats to high dose of LTG reflects hepatocellular damage that may lead to hepatitis.
It is concluded that regular liver function and drug monitoring should follow the treatment with LTG. 相似文献
12.
Manisha Mavai Yogendra Raj Singh R. C. Gupta Sandeep K. Mathur Bharti Bhandari 《Indian journal of clinical biochemistry : IJCB》2018,33(2):222-228
Autonomic activity may be deranged in thyroid dysfunctions and may lead to cardiovascular morbidity and mortality. Myopathy is a common manifestation in thyroid disorders and may be associated with raised serum creatine kinase (CK). We hypothesized that cardiovascular abnormality in thyroid dysfunction may manifest as raised CK-MB. This study was designed to investigate the correlation of CK and its isoform CK-MB with thyroid profile and linear parameters of heart rate variability (HRV). The study was conducted on 35 hypothyroid and hyperthyroid patients each, and 25 age-matched healthy controls. Autonomic activity was assessed by simple short term 5-min HRV. Biochemical evaluation of serum thyroid profile, CK-NAC and CK-MB were estimated in all the subjects. Our results demonstrated low HRV in hypo- as well as hyperthyroid patients. We observed significantly higher serum CK levels in hypothyroid patients when compared to hyperthyroids and controls. However, no significant differences were observed in CK-MB levels in the three groups. Significant positive correlation of CK with TSH and negative correlation with some HRV parameters (LF power, HF power, total power, SDNN, RMSSD) was observed in hypothyroid patients. Whereas correlation of CK-MB with thyroid profile as well as HRV parameters was non-significant in all the groups. Based on the CK and CK-MB findings and their correlation, we conclude that the cardiovascular changes seen in thyroid dysfunctions may primarily be due to autonomic imbalance without apparent cardiac muscle involvement. Whereas, raised CK levels indicate predominantly skeletal muscle involvement in hypothyroid patients. 相似文献
13.
Vivek K. Dwivedi Mahesh Chandra P. C. Misra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2005,20(1):21-25
Effect of administration of 600 mg. vitamin E each day, for six days, was observed on activity of some of the anti-oxidant
enzymes and levels of malondialdehyde (as an index of free radical mediated damage) in the platelets of patients reperfused
after myocardial infarction. It has been found that vitamin E administration significantly lowers the level of malondialdehyde
in the patients. Vitamin E administration increases the activities of anti oxidant enzymes (viz. superoxide dismutase, glutathione
reductase and catalase) tested both in the patients and healthy controls. Vitamin E administration causes general stimulation
of anti-oxidant enzyme activities both in healthy persons and the patients, however, lowering of lipid per-oxidation upon
administration of vitamin E is specific for patients. These findings exhibit beneficial role of vitamin E administration in
the management of the patients reperfused after myocardial infarction. 相似文献
14.
Uday Kumar Anand Sharan Shaheena Kamal 《Indian journal of clinical biochemistry : IJCB》2003,18(2):6-7
Usually CPK, CK-MB and lactate dehydrogenase (LDH) enzymes are measured in blood during the period of myocardial infarction.
The changes in LDH assay indicates duration of the infarction.
The level of lactate dehydrogenase is 500 times greater in tissue than those found in serum. A small mass of damaged tissue
causes leakage of enzyme and increases its level in serum.
Any cause of tissue break down/hemolysis which is sufficiently severe can produce LDH pattern similar to that in myocardial
infarction.
We report this case of small bowel volvulus showing significant increase in LDH.
It may be due to tissue necrosis which increases the level of LDH in serum. 相似文献
15.
Rashmi Choudhary K. P. Mishra C. Subramanyam 《Indian journal of clinical biochemistry : IJCB》2006,21(2):107-113
Recent reports on the involvement of calcineurin in cardiac hypertrophy and its susceptibility to free radicals, prompted
us to examine possible beneficial effects of dietary antioxidants in this regard. In continuation of initialin vitro studies revealing eugenol to be a potent calcineurin inhibitor, we investigated its ability to reverse isoproterenol-induced
cardiac hypertrophy in rats. Intraperitoneal administration of isoproterenol (1 mg/kg body wt/day for 10 days) induced cardiac
hypertrophy with increased heart weight and enhanced apoptosis of myocytes concomitant with accumulation of reactive oxygen
species, decreased glutathione contents, increased activities of calcineurin and protein kinase C in ventricular tissue. Administering
eugenol for 3 days (1 mg/kg body wt/twice a day), followed by combined administration of isoproterenol and eugenol resulted
in significant reversal of cardiac hypertrophy and restoration of above changes. These results suggest that eugenol, a natural
antioxidant of dietary origin, may offer potential benefits in the management of cardiac hypertrophy. 相似文献
16.
Mahalingam Gayathri Krishnan Kannabiran 《Indian journal of clinical biochemistry : IJCB》2008,23(4):394-400
The aim of the present study was to evaluate the antidiabetic and ameliorative potential of aqueous extract of Ficus bengalensis
bark in streptozotocin induced diabetic rats. The effect of oral administration of aqueous extract of F. bengalensis bark
on blood glucose, serum electrolytes, serum glycolytic enzymes, liver microsomal protein, hepatic cytochrome P-450 dependent
monooxygenase enzymes and lipid peroxidation in liver and kidney of streptozotocin -induced diabetic rats was studied. Oral
administration of Ficus bengalensis to fed, fasted and glucose loaded diabetic rats significantly [F > 0.05 (ANOVA) and P<
0.05 (DMRT)] decreased the blood glucose level at 5 hrs and restored the levels of serum electrolytes, glycolytic enzymes
and hepatic cytochrome P-450 dependent enzyme systems and decreased the formation of liver and kidney lipid peroxides at the
end of 12 weeks. Further, the aqueous extract of Ficus bengalensis at a dose of 500mg/kg/day exhibits significant antidiabetic
and ameliorative activity as evidenced by histological studies in normal and Ficus bengalensis treated streptozotocin induced
diabetic rats. On the basis of our findings, it could be used as an antidiabetic and ameliorative agent for better management
of diabetes mellitus. 相似文献
17.
Vishnu Kumar A. K. Khanna M. M. Khan Ranjana Singh Sushma Singh Ramesh Chander Farzana Mahdi J. K. Saxena Shweta Saxena V. K. Singh R. K. Singh 《Indian journal of clinical biochemistry : IJCB》2009,24(1):65-69
The present study was carried out to evaluate the hypoglycemic, lipid lowering and antioxidant activities in root extract
of Anthocephalus indicus (A indicus) in alloxan inducd diabetic rats. Oral administration of ethanol extract of root (500mg/
kg body weight) for 21 days resulted in significant decrease in the levels of blood glucose, triglycerides, total cholesterol,
phospholipid and free fatty acids. Furthermore, the root extract (100–400μg) inhibited the generation of superoxide anions
and hydroxyl radicals, in both enzymic and non-enzymic systems, in vitro. The result of the present study demonstrated hypoglycemic,
lipid lowering and antioxidant activities in root extract of A indicus, which could help in prevention of diabetic dyslipidemia
and related diseases. 相似文献
18.
Present study investigated the effects of isoproterenol-induced oxidative stress on hemodynamic and ventricular functions
in rats. Subcutaneous injections of isoproterenol (85 mg/kg for two consecutive days at 24 h interval) significantly decreased
myocardial antioxidant enzymes; superoxide dismutase, catalase and glutathione peroxidase in heart. Isoproterenol-induced
oxidative stress was also evidenced by significant depletion of reduced glutathione and increased formation of lipid peroxidation
product, thiobarbituric acid reactive substances along with depletion of myocyte injury specific marker enzymes; creatine
phosphokinase isoenzyme and lactate dehydrogenase. The deleterious outcome of oxidative stress on hemodyanmic parameters and
ventricular function were further evidenced by decreased systolic, diastolic and mean arterial blood pressure, heart rate,
ventricular contractility; [(+)LVdP/dt] and relaxation; [(−)LVdP/dt], along with an increased left ventricular end diastolic
pressure (LVEDP). Subsequent to changes in heart rate and arterial pressure, isoproterenol also decreased rate pressure product.
Present study findings clearly demonstrate the detrimental outcome of isoproterenol induced-oxidative stress on cardiac function
and tissue antioxidant defense and substantiate its suitability as an animal model for the evaluation of cardioprotective
agents. 相似文献
19.
P. V. Raj K. Nitesh H. R. Chandrashekhar C. Mallikarjuna Rao J. Venkata Rao N. Udupa 《Indian journal of clinical biochemistry : IJCB》2010,25(2):169-174
To investigate Lecithin for its hepatoprotective activity against D-galactosamine (D-GalN) induced toxicity in freshly isolated
rat hepatocytes and animal models. Freshly isolated rat hepatocytes were exposed to Dgalactosamine (30 mM) along with/without
lecithin (100 μg/ml) and the levels of selected liver enzymes were measured. Thirty six Wistar strain albino rats were used
for the in vivo investigations. Lecithin 50 and 100 mg/kg.b.wt were administered for one week by oral route. Liver damage was induced by
intra peritoneal administration of 400 mg/kg b.wt D-galactosamine. The antihepatotoxic effect of lecithin was observed in
freshly isolated rat hepatocytes at concentration 100 μg/ml and was found to be similar to that of the standard silymarin
used. Its in vivo hepatoprotective effect at 100 mg/kg b.wt was comparable with that of the standard silymarin at 100 mg/kg body weight. Lecithin
was able to normalise the biochemical levels which were altered due to D-galactosamine intoxication in freshly isolated rat
hepatocytes and also in animal models. 相似文献
20.
Present study aimed to evaluate the protective role of the aqueous extract of Phyllanthus niruri (P. niruri) against nimesulide-induced
hepatic disoder in mice by determining levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase
(GPT) and alkaline phosphatase (ALP) in serum and also by measuring the hepatic content of the antioxidant enzymes, superoxide
dismitase (SOD) and catalase (CAT); the free radical scavenger, reduced glutathione (GSH) and thiobarbituric acid reacting
substances (TBARS). Aqueous extract of P. niruri was administered either orally or intraperitoneally in different doses and
times as needed for the experiments. Intraperitoneal of the extract (100 mg/kg body weight for seven days) reduced nimesulide
(750 mg/kg body weight for 3 days) induced increased levels of GOT (37.0±1.8 units/ml in control group vs. 91.8±2.0 units/ml
in nimesulide treated group vs. 35.0±1.0 units/ml in extract treated group), GPT (30.0±2.1 units/ml in control group vs. 88.4±2.9
units/ml in nimesulide treated group vs. 34.1±1.8 units/ml in extract treated group), and ALP (7.86±0.47 KA units/ml in control
group vs. 23.80±0.60 KA units/ml in nimesulide treated group vs. 7.30±0.40 KA units/ml, in extract treated group) to almost
nomal. In addition, P. niruri restored the nimesulide induced alterations of hepatic SOD (550±20 units/mg total protein in
control group vs. 310±13 units/mg total protein in nimesulide treated group vs. 515±10 units/mg total protein in extract treated
group), CAT (99.5±2 units/mg total protein in control group vs. 25.0±1.5 units/mg total protein in nimesulide treated group
vs. 81.0±0.8 units/mg total protein in extract treated group), GSH (90±3 nmoles/mg total protein in control group vs. 17±4.2
nmoles/mg total protein in nimesulide treated group vs. 81±1 nmoles/mg total protein in extract treated group) and TBARS (measured
as MDA, 36.6±3.0 nmoles/g liver tissue in control group vs. 96.3±5.2 nmoles/g liver tissue in nimesulide treated group vs.
41.2±1.7 nmoles/g liver tissue in extract treated group) contents. Dose-dependent studies showed that the herb could protect
liver even if the nimesulide-induced injury is severe. Intraperitoneal administration of the extract showed better protective
effect than oral administration. Combining all, the data suggest that P. niruri possesses hepatoprotective activity against
nimesulide-induced liver toxicity and probably acts via an antioxidant defense mechanism. To the best of our knowledge, this
is the first report of the hepatoprotective action of P. niruri against nimesulide induced liver damage. 相似文献