地塞米松可以减少由重症急性胰腺炎引起的肾脏微血管内皮糖萼的损伤（英文）

目的:明确地塞米松可以减少重症急性胰腺炎(SAP)引起的肿瘤坏死因子(TNF-α)的释放,减轻TNF-α导致的肾脏血管内皮糖萼的降解,从而改善肾脏微循环和缓解肾损伤。创新点:本研究通过小鼠活体研究的方法,建立小鼠重症急性胰腺炎模型,并用地塞米松进行干预对照,采用透射电镜、激光多谱勒和酶联免疫的方法,检测了各组小鼠肾脏血管内皮糖萼的完整性、肾血流灌注和TNF-α表达情况,阐明了地塞米松对内皮糖萼的保护作用。方法:通过"胰管结扎+腹腔内雨蛙素注射"的方法建立SAP模型,分别留取各组小鼠的血液和组织标本,采用透射电镜观察内皮糖萼的损伤情况,用酶联免疫检测血清TNF-α和糖萼成份多配体聚糖的浓度,并用激光多谱勒检测活体小鼠肾脏的灌注,分析地塞米松对内皮糖萼的保护和改善肾脏灌注的作用。结论:SAP可以引起TNF-α的大量释放,并导致内皮糖萼的降解和肾脏灌注下降,而地塞米松可以减少TNF-α的释放,减轻糖萼的降解,改善肾脏血流灌注。     

Study progress in therapeutic effects of traditional Chinese medicine monomer in severe acute pancreatitis

INTRODUCTION Severe acute pancreatitis (SAP) is one of the three main acute abdominal problems always ac-companied by multiple-system and multiple-organ impaired function or failure. Its onset and develop-ment are characterized by rapid changes, complicated illness state and difficult treatment. With the con-tinuous development of the traditional Chinese medicine industry in recent years, it is proved that traditional Chinese medicine (TCM) monomers monomers have a marked effect for …     

Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice

Objective: To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae (Danshen) in the treatment of severe acute panereatitis (SAP)- or obstructive jaundice (OJ)-induced heart injury. Methods: A total of 288 rats were used for SAP-(n=108) and O J-associated (n=180) experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups (n=12), whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups (n=15). At the corre-sponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A2 (PLA2) in blood, and pathological changes of the hearts were investigated. Results: The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly (P>0.05). The contents ofendotoxin (at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively) and PLA2 (at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively) in the treated group were significantly lower than those in the model control group (P<0.01 and P<0.001, respec-tively). Besides, myocardial pathological injuries were mitigated in SAP and OJ rats. Conclusion: In this study, we found that Salvia miltiorrhizae improved myocardial pathological changes, reduced the content of PLA2 in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats.     

Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice

Objective  To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae (Danshen) in the treatment of severe acute pancreatitis (SAP)- or obstructive jaundice (OJ)-induced heart injury. Methods  A total of 288 rats were used for SAP- (n=108) and OJ-associated (n=180) experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups (n=12), whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups (n=15). At the corresponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A₂ (PLA₂) in blood, and pathological changes of the hearts were investigated. Results  The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly (P>0.05). The contents of endotoxin (at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively) and PLA₂ (at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively) in the treated group were significantly lower than those in the model control group (P<0.01 and P<0.001, respectively). Besides, myocardial pathological injuries were mitigated in SAP and OJ rats. Conclusion  In this study, we found that Salvia miltiorrhizae improved myocardial pathological changes, reduced the content of PLA₂ in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats. Projected supported by the Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province (Nos. 2003C130 and 2004C142), the Grave Foundation Project for Technological and Development of Hangzhou City (No. 2003123B19), and the Intensive Foundation Project for Technology of Hangzhou City (No. 2004Z006), China     

Review:Study progress in therapeutic effects of traditional Chinese medicine monomer in severe acute pancreautis

Severe acute pancreatitis (SAP) is a common acute abdomen clinical problem characterized by high mortality, multiple complications, complicated pathogenesis and difficult treatment. Recent studies found traditional Chinese medicine (TCM)monomers have markedly good effect for treating SAP. Many TCM monomers can inhibit pancreatin, resist inflammation, improve microcirculation and immunoloregulation, etc. to block the pathological progress of SAP in multiple ways, reduce complications and lower mortality with rapid effects. It is significant for enhancing SAP treatment to deeply understand the current situation in TCM monomers for treating SAP and take precious references therein. This article summarizes the treating effects and mechanisms of TCM monomers for SAP in recent years.     

Review: Study progress on mechanism of severe acute pancreatitis complicated with hepatic injury

Study on the action mechanism of inflammatory mediators generated by the severe acute pancreatitis (SAP) in multiple organ injury is a hotspot in the surgical field. In clinical practice, the main complicated organ dysfunctions are shock, respiratory failure, renal failure, encephalopathy, with the rate of hepatic diseases being closely next to them. The hepatic injury caused by SAP cannot only aggravate the state of pancreatitis, but also develop into hepatic failure and cause patient death. Its complicated pathogenic mechanism is an obstacle in clinical treatment. Among many pathogenic factors, the changes ofvasoactive substances, participation of inflammatory mediators as well as OFR (oxygen free radical), endotoxin, etc. may play important roles in its progression.     

急性重症胰腺炎的临床治疗分析

目的研究急性重症胰腺炎(ASP)的临床治疗.方法对34例ASP的临床资料进行回顾性分析.结果全组34例,治愈27例,治愈率为79.41%.非手术治疗组治愈率为88.89%,手术治疗组治愈率为68.75%.结论ASP非手术治疗的治愈率明显高于手术治疗.早期诊断ASP对选择ASP的治疗方式及改善预后有重要意义.     

Study progress on mechanism of severe acute pancreatitis complicated with hepatic injury

Study on the action mechanism of inflammatory mediators generated by the severe acute pancreatitis(SAP) in mul-tiple organ injury is a hotspot in the surgical field.In clinical practice,the main complicated organ dysfunctions are shock,res-piratory failure,renal failure,encephalopathy,with the rate of hepatic diseases being closely next to them.The hepatic injury caused by SAP cannot only aggravate the state of pancreatitis,but also develop into hepatic failure and cause patient death.Its complicated pathogenic mechanism is an obstacle in clinical treatment.Among many pathogenic factors,the changes of vasoac-tive substances,participation of inflammatory mediators as well as OFR(oxygen free radical),endotoxin,etc.may play important roles in its progression.     

奥曲肽治疗急性水肿性胰腺炎的疗效观察——附68例临床分析

目的 :评价奥曲肽 (善宁 ,Sandostatin)对急性水肿性胰腺炎 (AEP)的治疗价值及应用指征 .方法 :将 15 2例AEP按年龄及有否加用奥曲肽分为 4组 .按下列指标评估 :腹痛消失时间、血淀粉酶复常时间、转为急性重症胰腺炎 (ANP)发生率、手术率、病死率 .结果 :大于 5 5岁的两组 ,腹痛消失时间奥曲肽治疗组 (治疗组 )为 (2 6± 1 5 )天 ,对照组为 (4 7± 2 3)天 ,p <0 0 5 ;血淀粉酶复常时间治疗组为 (3 1± 1 6 )天 ;对照组为 (4 6± 2 3)天 ,P <0 0 5 ;转为ANP的发生率 ,治疗组为 6 3% (2 /32 ) ,对照组为 2 5 6 % (10 /39) ,p <0 0 5 ;转手术率治疗组为 3 1% (1/32 ) ,对照组为10 3% (4/39) ,p >0 0 5 .小于或等于 5 5岁的两组 ,腹痛消失时间和血淀粉酶复常时间均无统计学差别 .结论 :AEP高龄患者早期应用奥曲肽治疗 ,能缩短病程 ,改善预后 ,减少ANP的发生     

Clinical and immune response characteristics among vaccinated persons infected with SARS-CoV-2 delta variant: a retrospective study

ObjectiveThis study aimed to observe the clinical and immune response characteristics of vaccinated persons infected with the delta variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Yangzhou, China.MethodsWe extracted the medical data of 129 patients with delta-variant infection who were admitted to Northern Jiangsu People’s Hospital (Yangzhou, China) between August and September, 2021. The patients were grouped according to the number of vaccine doses received into an unvaccinated group: a one-dose group and a two-dose group. The vaccine used was SARS-CoV-2-inactivated vaccine developed by Sinovac. We retrospectively analyzed the patients’ epidemiological, clinical, laboratory, and imaging data.ResultsAlmost all patients with delta-variant infection in Yangzhou were elderly, and patients with severe/critical illness were over 70 years of age. The rates of severe/critical illness (P=0.006), fever (P=0.025), and dyspnea (P=0.045) were lower in the two-dose group than in the unvaccinated group. Compared to the unvaccinated group, the two-dose group showed significantly higher lymphocyte counts and significantly lower levels of C-reactive protein (CRP), interleukin-6 (IL-6), and D-dimer during hospitalization and a significantly higher positive rate of immunoglobulin G (IgG) antibodies at admission (all P<0.05). The cumulative probabilities of hospital discharge and negative virus conversion were also higher in the two-dose group than in the unvaccinated group (P<0.05).ConclusionsTwo doses of the SARS-CoV-2-inactivated vaccine were highly effective at limiting symptomatic disease and reducing immune response, while a single dose did not seem to be effective.     

Mechanism of acute pancreatitis complicated with injury of intestinal mucosa barrier

Acute pancreatitis （AP） is a common acute abdomen in clinic with a rapid onset and dangerous pathogenetic condition AP can cause an injury of intestinal mucosa barrier, leading to translocation of bacteria or endotoxin through multiple routes, bacterial translocation （BT）, gutorigin endotoxaemia, and secondary infection of pancreatic tissue, and then cause systemic inflammatory response syndrome （SIRS） or multiple organ dysfunction syndrome （MODS）, which are important factors influencing AP＇s severity and mortality. Meanwhile, the injury of intestinal mucosa barrier plays a key role in AP＇s process. Therefore, it is clinically important to study the relationship between the injury of intestinal mucosa barrier and AP. In addition, many factors such as microcirculation disturbance, ischemical reperfusion injury, excessive release of inflammatory mediators and apoptosis may also play important roles in the damage of intestinal mucosa barrier. In this review, we summarize studies on mechanisms of AP.     

Review:Mechanism of acute pancreatitis complicated with injury of intestinal mucosa barrier

Acute pancreatitis (AP) is a common acute abdomen in clinic with a rapid onset and dangerous pathogenetic condition. AP can cause an injury of intestinal mucosa barrier, leading to translocation of bacteria or endotoxin through multiple routes, bacterial translocation (BT), gutorigin endotoxaemia, and secondary infection of pancreatic tissue, and then cause systemic in- flammatory response syndrome (SIRS) or multiple organ dysfunction syndrome (MODS), which are important factors influencing AP’s severity and mortality. Meanwhile, the injury of intestinal mucosa barrier plays a key role in AP’s process. Therefore, it is clinically important to study the relationship between the injury of intestinal mucosa barrier and AP. In addition, many factors such as microcirculation disturbance, ischemical reperfusion injury, excessive release of inflammatory mediators and apoptosis may also play important roles in the damage of intestinal mucosa barrier. In this review, we summarize studies on mechanisms of AP.     

Chaiqin Chengqi Decoction decreases IL-6 levels in patients with acute pancreatitis

Objective  

In this paper, we investigated the effect of the traditional Chinese medicine Chaiqin Chengqi Decoction (CQCQD) on serum cytokines in acute pancreatitis (AP) patients.     

Relationship between resistin level in serum and acute coronary syndrome or stable angina pectoris

Objective To investigate the relationship between serum resistin level and acute coronary syndrome (ACS) or stable angina pectoris (SAP). Methods Sixty-five patients, with coronary artery disease, were enrolled and divided into three subgroupsacute myocardial infarction (AMI), unstable angina pectoris (UAP) and SAP, and 26 healthy people were recruited as controls in the cross-sectional study. Serum resistin levels were determined by ELISA (enzyme-linked immunosorbent assay), and WBC (white blood cell count), hsCRP (high sensitive C-reaction protein), CKmax (maximum ofcreatinkinase), CK-MBmax (maximum of isozyme of creatinkinase) and cTnImax (maximum oftroponin) were measured by standard laboratory methods. Results The serum resistin levels were 4 folds higher in AMI patients, 2.43 folds in UAP patients and 1.12 folds in SAP patients than in the healthy controls (P＜0.05). The resistin levels were also significantly different between AMI [(8.16±0.79) ng/ml], UAP [(5.59±0.75) ng/ml]and SAP [(3.45±0.56) ng/ml] groups (P＜0.01); WBC, hsCRP, CKmax, CK-MBmax and cTnImax were significantly increased in AMI patients over UAP and SAP patients. Spearman analysis showed that serum resistin levels were positively correlated with WBC (r=0.412, P=0.046), hsCRP (r=0.427, P=0.037), CKmax, CK-MBmax and cTnImax (r=0.731, 0.678, 0.656; P＜0.01). ConclusionSerum resistin levels increased with inflammatory factors and myocardial impairment. The results suggest that human resistin might play an important role in the pathogenesis of atherosclerosis and AMI as an inflammatory factor.     

Acute pancreatitis associated left-sided portal hypertension with severe gastrointestinal bleeding treated by transcatheter splenic artery embolization: a case report and literature review

Left-sided portal hypertension (LSPH) followed by acute pancreatitis is a rare condition with most patients being asymptomatic. In cases where gastrointestinal (GI) bleeding is present, however, the condition is more complicated and the mortality is very high because of the difficulty in diagnosing and selecting optimal treatment. A successfully treated case with severe GI bleeding by transcatheter splenic artery embolization is reported in this article. The patient exhibited severe uncontrollable GI bleeding and was confirmed as gastric varices secondary to LSPH by enhanced computed tomography (CT) scan and CT-angiography. After embolization, the bleeding stopped and stabilized for the entire follow-up period without any severe complications. In conclusion, embolization of the splenic artery is a simple, safe, and effective method of controlling gastric variceal bleeding caused by LSPH in acute pancreatitis.     

朊病毒及朊病毒病的研究进展

198 2年 ,美国生理学家Ｐｒｕｓｉｎｅｒ及其同事 ,在大量实验的基础上 ,突破经典病毒学理论而提出朊病毒(ｐｒｉｏｎ)的概念 ,认为绵羊瘙痒病的病原体是一种尚未证实有核酸结构的蛋白质侵染颗粒 (ｐｒｏｔｅｉｎａｃｅｏｕｓｉｎ ｆｅｃｔｉｏｕｓｐａｒｔｉｃｌｅｓ) ,并将其称为朊病毒蛋白 (ｐｒｉｏｎｐｒｏ ｔｅｉｎ) ,简称为ＰｒＰ。朊病毒是医学生物学领域中至今尚未彻底弄清 ,与病毒和类病毒都很不相同的一种蛋白质传染病原体。它是一组至今不能查到任何核酸、对各种理化作用具有很强抵抗力、传染性甚强、分子重量在 2 70 0 0～ 30 …     

植物原生质体培养与融合的研究进展

对原生质体的分离、培养、融合及植株再生方面进行了简要的论述.并根据对有关文献的分析,讨论了原生质体研究中存在的问题及今后的发展趋势,指出植物原生质体的游离、培养和融合技术在近20多年来得到了迅速的发展,今后在进一步加强原生质体培养基础理论研究的同时,重点放在利用原生质体融合技术进行遗传性状的改良,以获得优良性状的个体并通过无性途径固定下来.     

25例急性胰腺炎中西医结合临床治疗观察

目的:探讨在西医治疗的基础上,加用中药治疗急性胰腺炎疗效观察。方法:采取西医治疗及口服中药胰腺消炎汤临证分型,辩证论治治疗急性胰腺炎25例。结果:25例中10例瘀滞型全部治愈;11例热厥型:7例经药物治疗痊愈,4例药物治疗不明显,病情严重立即转他院手术治疗,其中3例治愈,1例死亡(并发败血症)。结论:中西医相结合可提高对急性胰腺炎的治疗效果。     

生物衰老机理研究进展

衰老作为生物生长发育的必经阶段,历来受到人们的关注。随着研究水平的不断提高,人们对衰老机理的研究也日益深入, 学说纷呈。本文对衰老机理的主要学说进行了阐述。     

DNA单链断裂损伤修复研究进展

在哺乳动物细胞中DNA单链断裂的修复过程需要多聚（ADP-核糖）聚合酶、X射线修复交叉互补蛋白1、DNA连接酶IIIα和其他DNA损伤修复因子的参与。该修复过程分为单链断裂的检测、断链末端处理、缺口填补和DNA连接四个环节。