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拟青霉(Paecilomyces sp.FLH30)β-1,3(4)-葡聚糖酶可在重组菌BL21(DE3)-pET28a/PsBg16A中表达.首先确定了该酶的细胞表达定位,再研究了诱导温度、诱导剂种类及浓度、诱导起始菌体密度、诱导时间等因素对重组菌生长及目的蛋白表达活性的影响.结果表明,IPTG和乳糖皆可诱导目的蛋白表达,乳糖的诱导效果优于IPTG.在诱导起始OD_(600)为3.2时加入10%乳糖,20℃诱导20 h最适于目的蛋白的表达.表达条件优化后,酶活可达到482.2U/mL. 相似文献
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目的:在大肠杆菌(BL21)中构建可溶性表达的金黄色葡萄球菌B型肠毒素(SEB)受体拮抗剂。方法:首先确定SEB受体桔抗剂的基因序列,然后用含有SEB受体桔抗剂的基因序列重组质粒表达载体PGEX-4T-1转化大肠杆菌BL21(DE3),利用IPTG诱导表达获得蛋白,产物经GST柱纯化后,利用ELISA检测其与SEB的结合能力并进行其体内外药效学实验。结果:该质粒成功转化为可溶性表达,ELISA结果显示表达产物可与SEB特异性结合。结论:本研究成功对SEB受体拮抗剂GST可溶性表达并对其活性进行初步分析。 相似文献
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Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea 总被引:1,自引:0,他引:1
INTRODUCTION Fungi cause most plant diseases of all groups of microbes. During their infection cycles, fungal pathogens must undergo two key processes: first, penetration through cuticles into host plant cells; second, colonization in host cells utilizing nutrients from their hosts. To penetrate host cells, fungi de-velop a series of specialized infection structures such as appressorium, penetration peg, and infection hypha. The appressorium-mediated penetration is a process typical of s… 相似文献
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Wang LR Zhang GB Chen J Li J Li MW Xu N Wang Y Shen Tu JZ 《Journal of Zhejiang University. Science. B》2011,12(3):174-179