Validation of a single-stage submaximal treadmill walking test

Abstract

The single-stage treadmill walking test of Ebbeling et al. is commonly used to predict maximal oxygen consumption ([Vdot]O_2max) from a submaximal effort between 50% and 70% of the participant's age-predicted maximum heart rate. The purpose of this study was to determine if this submaximal test correctly predicts [Vdot]O_2max at the low (50% of maximum heart rate) and high (70% of maximum heart rate) ends of the specified heart rate range for males and females aged 18 – 55 years. Each of the 34 participants completed one low-intensity and one high-intensity trial. The two trials resulted in significantly different estimates of [Vdot]O_2max (low-intensity trial: mean 40.5 ml · kg^?1 · min^?1, s = 9.3; high-intensity trial: 47.5 ml · kg^?1 · min^?1, s = 8.8; P < 0.01). A subset of 22 participants concluded their second trial with a [Vdot]O_2max test (mean 47.9 ml · kg^?1 · min^?1, s = 8.9). The low-intensity trial underestimated (mean difference = ?3.5 ml · kg^?1 · min^?1; 95% CI = ?6.4 to ?0.6 ml · kg^?1 · min^?1; P = 0.02) and the high-intensity trial overestimated (mean difference = 3.5 ml · kg^?1 · min^?1; 95% CI = 1.1 to 6.0 ml · kg^?1 · min^?1; P = 0.01) the measured [Vdot]O_2max. The predictive validity of Ebbeling and colleagues' single-stage submaximal treadmill walking test is diminished when performed at the extremes of the specified heart rate range.     

No effect of glutamine supplementation and hyperoxia on oxidative metabolism and performance during high-intensity exercise

Abstract

Glutamine enhances the exercise-induced expansion of the tricarboxylic acid intermediate pool. The aim of the present study was to determine whether oral glutamine, alone or in combination with hyperoxia, influenced oxidative metabolism and cycle time-trial performance. Eight participants consumed either placebo or 0.125 g · kg body mass^?1 of glutamine in 5 ml · kg body mass^?1 placebo 1 h before exercise in normoxic (control and glutamine respectively) or hyperoxic (FiO₂ = 50%; hyperoxia and hyperoxia + glutamine respectively) conditions. Participants then cycled for 6 min at 70% maximal oxygen uptake ([Vdot]O_2max) immediately before completing a brief high-intensity time-trial (～4 min) during which a pre-determined volume of work was completed as fast as possible. The increment in pulmonary oxygen uptake during the performance test (Δ[Vdot]O_2max, P = 0.02) and exercise performance (control: 243 s, s _x  = 7; glutamine: 242 s, s _x  = 3; hyperoxia: 231 s, s _x  = 3; hyperoxia + glutamine: 228 s, s _x  = 5; P < 0.01) were significantly improved in hyperoxic conditions. There was some evidence that glutamine ingestion increased Δ[Vdot]O_2max in normoxia, but not hyperoxia (interaction drink/FiO₂, P = 0.04), but there was no main effect or impact on performance. Overall, the data show no effect of glutamine ingestion either alone or in combination with hyperoxia, and thus no limiting effect of the tricarboxylic acid intermediate pool size, on oxidative metabolism and performance during maximal exercise.     

Effect of a carbohydrate mouthwash on running time-trial performance

Abstract

The aim of the present study was to determine the effect of a carbohydrate mouthwash on running time-trial performance. On two separate occasions, seven recreationally active males ([Vdot]O_2max 57.8 ml · kg^?1 · min^?1, s = 3.7) completed a preloaded (15 min at 65%[Vdot]O_2max) time-trial of 45 min in duration on a motorized treadmill. At 6-min intervals during the preload and time-trial, participants were given either a 6% maltodextrin, 3% lemon juice solution (carbohydrate trial) or a 3% lemon juice placebo mouthwash (placebo trial) in a double-blind, randomized crossover design. Heart rate, oxygen consumption ([Vdot]O₂), respiratory exchange ratio (RER), and ratings of perceived exertion (RPE) were measured during the preload, and blood glucose and lactate were measured before and after the preload and time-trial. There were no significant differences in distance covered between trials (carbohydrate: 9333 m, s = 988; placebo: 9309 m, s = 993). Furthermore, there were no significant between-trial differences in heart rate and running speed during the time-trial, or [Vdot]O₂, RER or RPE during the preload. Blood lactate and glucose increased as a result of the exercise protocol, with no between-trial differences. In conclusion, there was no positive effect of a carbohydrate mouthwash on running performance of ～1 h duration.     

Effect of a novel pedal design on maximal power output and mechanical efficiency in well-trained cyclists

Abstract

In this study, we evaluated the effects of a novel pedal design, characterized by a downward and forward shift of the cleat fixing platform relative to the pedal axle, on maximal power output and mechanical efficiency in 22 well-trained cyclists. Maximal power output was measured during a series of short (5-s) intermittent sprints on an isokinetic cycle ergometer at cadences from 40 to 120 rev · min^?1. Mechanical efficiency was evaluated during a submaximal incremental exercise test on a bicycle ergometer using continuous [Vdot]O₂ and [Vdot]CO₂ measurement. Similar tests with conventional pedals and the novel pedals, which were mounted on the individual racing bike of the participant, were randomized. Maximal power was greater with novel pedals than with conventional pedals (between 6.0%, s_x  = 1.5 at 40 rev · min^?1 and 1.8%, s_x  = 0.7 at 120 rev · min^?1; P = 0.01). Torque production between crank angles of 60° and 150° was higher with novel pedals than with conventional pedals (P = 0.004). The novel pedal design did not affect whole-body [Vdot]O₂ or [Vdot]CO₂. Mechanical efficiency was greater with novel pedals than with conventional pedals (27.2%, s_x  = 0.9 and 25.1%, s_x  = 0.9% respectively; P = 0.047; effect size = 0.9). In conclusion, the novel pedals can increase maximal power output and mechanical efficiency in well-trained cyclists.     

Maximal oxygen uptake versus maximal power output in children

Abstract

Maximal oxygen uptake ([Vdot]O_2max) is considered the optimal method to assess aerobic fitness. The measurement of [Vdot]O_2max, however, requires special equipment and training. Maximal exercise testing with determination of maximal power output offers a more simple approach. This study explores the relationship between [Vdot]O_2max and maximal power output in 247 children (139 boys and 108 girls) aged 7.9–11.1 years. Maximal oxygen uptake was measured by indirect calorimetry during a maximal ergometer exercise test with an initial workload of 30 W and 15 W · min^?1 increments. Maximal power output was also measured. A sample (n = 124) was used to calculate reference equations, which were then validated using another sample (n = 123). The linear reference equation for both sexes combined was: [Vdot]O_2max (ml · min^?1) = 96 + 10.6 · maximal power + 3.5 · body mass. Using this reference equation, estimated [Vdot]O_2max per unit of body mass (ml · min^?1 · kg^?1) calculated from maximal power correlated closely with the direct measurement of [Vdot]O_2max (r = 0.91, P <0.001). Bland-Altman analysis gave a mean limits of agreement of 0.2±2.9 (ml · min^?1 · kg^?1) (1 s). Our results suggest that maximal power output serves as a good surrogate measurement for [Vdot]O_2max in population studies of children aged 8–11 years.     

The effects of work:rest duration on physiological and perceptual responses during intermittent exercise and performance

Abstract

In this study, we examined the effects of different work:rest durations during 20 min intermittent treadmill running and subsequent performance. Nine males (mean age 25.8 years, s = 6.8; body mass 73.9 kg, s = 8.8; stature 1.75 m, s = 0.05; [Vdot]O_2max 55.5 ml · kg^?1 · min^?1, s = 5.8) undertook repeated sprints at 120% of the speed at which [Vdot]O_2max was attained interspersed with passive recovery. The work:rest ratio was constant (1:1.5) with trials involving either short (6:9 s) or long (24:36 s) work:rest exercise protocols (total exercise time 8 min). Each trial was followed by a performance run to volitional exhaustion at the same running speed. Testing order was randomized and counterbalanced. Heart rate, oxygen consumption, respiratory exchange ratio, and blood glucose were similar between trials (P > 0.05). Blood lactate concentration was greater during the long than the short exercise protocol (P < 0.05), whereas blood pH was lower during the long than the short exercise protocol (7.28, s = 0.11 and 7.30, s = 0.03 at 20 min, respectively; P < 0.05). Perceptions of effort were greater throughout exercise for the long than the short exercise protocol (16.6, s = 1.4 and 15.1, s = 1.6 at 20 min, respectively; P < 0.05) and correlated with blood lactate (r = 0.43) and bicarbonate concentrations (r = ?0.59; P < 0.05). Although blood lactate concentration at 20 min was related to performance time (r = ?0.56; P < 0.05), no differences were observed between trials for time to exhaustion (short exercise protocol: 95.8 s, s = 30.0; long exercise protocol: 92.0 s, s = 37.1) or physiological responses at exhaustion (P > 0.05). Our results demonstrate that 20 min of intermittent exercise involving a long work:rest duration elicits greater metabolic and perceptual strain than intermittent exercise undertaken with a short work:rest duration but does not affect subsequent run time to exhaustion.     

Criterion-related validity of the 20-m shuttle run test in youths aged 13–19 years

Abstract

We assessed the agreement between maximal oxygen consumption ([Vdot]O_2max) measured directly when performing the 20-m shuttle run test and estimated [Vdot]O_2max from five different equations (i.e. Barnett, equations a and b; Léger; Matsuzaka; and Ruiz) in youths. The 20-m shuttle run test was performed by 26 girls (mean age 14.6 years, s = 1.5; body mass 57.2 kg, s = 8.9; height 1.60 m, s = 0.06) and 22 boys (age 15.0 years, s = 1.6; body mass 63.5 kg, s = 11.5; height 1.70 m, s = 0.01). The participants wore a portable gas analyser (K4b2, Cosmed) to measure [Vdot]O₂ during the test. All the equations significantly underestimated directly measured [Vdot]O_2max, except Barnett's (b) equation. The mean difference ranged from 1.3 ml · kg^?1 · min^?1 (Barnett (b)) to 5.5 ml · kg^?1 · min^?1 (Léger). The standard error of the estimate ranged from 5.3 ml · kg^?1 · min^?1 (Ruiz) to 6.5 ml · kg^?1 · min^?1 (Léger), and the percentage error ranged from 21.2% (Ruiz) to 38.3% (Léger). The accuracy of the equations available to estimate [Vdot]O_2max from the 20-m shuttle run test is questionable at the individual level. Furthermore, special attention should be paid when comparisons are made between studies (e.g. population-based studies) using different equations. The results of the present study suggest that Barnett's (b) equation provides the closest agreement with directly measured [Vdot]O_2max (cardiorespiratory fitness) in youth.     

The influence of drink temperature on thermoregulatory responses during prolonged exercise in a moderate environment

Abstract

Nine males cycled at 53% (s = 2) of their peak oxygen uptake ([Vdot]O_2peak) for 90 min (dry bulb temperature: 25.4°C, s = 0.2; relative humidity: 61%, s = 3). One litre of flavoured water at 10 (cold), 37 (warm) or 50°C (hot) was ingested 30 – 40 min into exercise. Immediately after the 90 min of exercise, participants cycled at 95%[Vdot]O_2peak to exhaustion to assess exercise capacity. Rectal and mean skin temperatures and heart rate were recorded. The gradient of rise in rectal temperature was influenced (P < 0.01) by drink temperature. Mean skin temperature was highest in the hot trial (cold trial: 34.2°C, s = 0.5; warm trial: 34.4°C, s = 0.5; hot trial: 34.7°C, s = 0.6; P < 0.01). Significant differences were observed in heart rate (cold trial: 132 beats · min^?1, s = 13; warm trial: 134 beats · min^?1, s = 12; hot trial: 139 beats · min^?1, s = 13; P < 0.05). Exercise capacity was similar between trials (cold trial: 234 s, s = 69; warm trial: 214 s, s = 52; hot trial: 203 s, s = 53; P = 0.562). The heat load and debt induced via drinking resulted in appropriate thermoregulatory reflexes during exercise leading to an observed heat content difference of only 33 kJ instead of the predicted 167 kJ between the cold and hot trials. These results suggest that there may be a role for drink temperature in influencing thermoregulation during exercise.     

Repeatability of scores on a novel test of endurance running performance

Abstract

The aim of the present study was to determine the repeatability of a running endurance test using an automated treadmill system that requires no manual input to control running speed. On three separate occasions, 7 days apart, 10 experienced male endurance-trained runners (mean age 32 years, s = 10; [Vdot]O_2peak 61 ml · kg^?1 · min^?1, s = 7) completed a treadmill time trial, in which they were instructed to run as far as possible in 60 min. The treadmill was instrumented with an ultrasonic feedback-controlled radar modulator that spontaneously regulated treadmill belt speed corresponding to the changing running speed of each runner. Estimated running intensity was 70%[Vdot]O_2peak (s = 11) and the distance covered 13.5 km (s = 2), with no difference in mean performances between trials. The coefficient of variation, estimated using analysis of variance, with participant and trial as main effects, was 1.4%. In summary, the use of an automated treadmill system improved the repeatability of a 60-min treadmill time trial compared with time trials in which speed is controlled manually. The present protocol is a reliable method of assessing endurance performance in endurance-trained runners.     

The effect of induced alkalosis and submaximal cycling on neuromuscular response during sustained isometric contraction

Abstract

The aim of this study was to determine if inducing metabolic alkalosis would alter neuromuscular control after 50 min of standardized submaximal cycling. Eight trained male cyclists (mean age 32 years, s = 7; [Vdot]O_2max 62 ml · kg^?1 · min^?1, s = 8) ingested capsules containing either CaCO₃ (placebo) or NaHCO₃ (0.3 g · kg^?1 body mass) in eight doses over 2 h on two separate occasions, commencing 3 h before exercise. Participants performed three maximal isometric voluntary contractions (MVC) of the knee extensors while determining the central activation ratio by superimposing electrical stimulation both pre-ingestion and post-exercise, followed by a 50-s sustained maximal contraction in which force, EMG amplitude, and muscle fibre conduction velocity were assessed. Plasma pH, blood base excess, and plasma HCO₃ were higher (P < 0.01) during the NaHCO₃ trial. After cycling, muscle fibre conduction velocity was higher (P < 0.05) during the 50-s sustained maximal contraction with NaHCO₃ than with placebo (5.1 m · s^?1, s = 0.4 vs. 4.2 m · s^?1, s = 0.4) while the EMG amplitude remained the same. Force decline rate was less (P < 0.05) during alkalosis-sustained maximal contraction and no differences were shown in central activation ratio. These data indicate that induced metabolic alkalosis can increase muscle fibre conduction velocity following prolonged submaximal cycling.     

Cardiopulmonary loading in motocross riding

Abstract

The present study was designed to examine physiological responses during motocross riding. Nine Finnish A-level motocross riders performed a 15-min ride at a motocross track and a test of maximal oxygen uptake ([Vdot]O_2max) in the laboratory. Cardiopulmonary strain was measured continuously during the ride as well as in the [Vdot]O_2max test. During the ride, mean [Vdot]O₂ was 32 ml · kg^?1 · min^?1 (s = 4), which was 71% (s = 12) of maximum, while ventilation (V _E) was 73% (s = 15) of its maximum. The relative [Vdot]O₂ and V _E values during the riding correlated with successful riding performance (r = 0.80, P < 0.01 and r = 0.79, P < 0.01, respectively). Mean heart rate was maintained at 95% (s = 7) of its maximum. Mean blood lactate concentration was 5.0 mmol · l^?1 (s = 2.0) after the ride. A reduction of 16% (P < 0.001) in maximal isometric handgrip force was observed. In conclusion, motocross causes riders great physical stress. Both aerobic and anaerobic metabolism is required for the isometric and dynamic muscle actions experienced during a ride.     

The interactive effect of exercise and immunosuppressant cyclosporin A on immune function in mice

Abstract

The determination of the ventilatory threshold has been a persistent problem in research and clinical practice. Several computerized methods have been developed to overcome the subjectivity of visual methods but it remains unclear whether different computerized methods yield similar results. The purpose of this study was to compare nine regression-based computerized methods for the determination of the ventilatory threshold. Two samples of young and healthy volunteers (n = 30 each) participated in incremental treadmill protocols to volitional fatigue. The ventilatory data were averaged in 20-s segments and analysed with a computer program. Significant variance among methods was found in both samples (Sample 1: F = 11.50; Sample 2: F = 11.70, P < 0.001 for both). The estimates of the ventilatory threshold ranged from 2.47 litres · min^?1 (71%[Vdot]O_2max) to 3.13 litres · min^?1 (90%[Vdot]O_2max) in Sample 1 and from 2.37 litres · min^?1 (67%[Vdot]O_2max) to 3.03 litres · min^?1 (83%[Vdot]O_2max) in Sample 2. The substantial differences between methods challenge the practice of relying on any single computerized method. A standardized protocol, likely based on a combination of methods, might be necessary to increase the methodological consistency in both research and clinical practice.     

The influence of serial feeding of drinks at different temperatures on thermoregulatory responses during cycling

Abstract

In this study, we examined thermoregulatory responses to ingestion of separate aliquots of drinks at different temperatures during low-intensity exercise in conditions of moderate heat stress. Eight men cycled at 50% (s = 3) of their peak oxygen uptake ([Vdot]O_2peak) for 90 min (dry bulb temperature: 25.3°C, s = 0.5; relative humidity: 60%, s = 5). Four 400-ml aliquots of flavoured water at 10°C (cold), 37°C (warm) or 50°C (hot) were ingested after 30, 45, 60, and 75 min of exercise. Immediately after the 90 min of exercise, participants cycled at 95%[Vdot]O_2peak to exhaustion to assess exercise capacity. There were no differences between trials in rectal temperature at the end of the 90 min of exercise (cold: 38.11°C, s = 0.30; warm: 38.10°C, s = 0.33; hot: 38.21°C, s = 0.30; P = 0.765). Mean skin temperature between 30 and 90 min tended to be influenced by drink temperature (cold: 34.49°C, s = 0.64; warm: 34.53°C, s = 0.69; hot: 34.71°C, s = 0.48; P = 0.091). Mean heart rate from 30 to 90 min was higher in the hot trial (129 beats · min^?1, s = 7; P < 0.05) than on the cold (124 beats · min^?1, s = 9) and warm trials (126 beats · min^?1, s = 8). Ratings of thermal sensation were higher on the hot trial than on the cold trial at 35 and 50 min (P < 0.05). Exercise capacity was similar between trials (P = 0.963). The heat load and debt induced by periodic drinking resulted in similar body temperatures during low-intensity exercise in conditions of moderate heat stress due to appropriate thermoregulatory reflexes.     

The effects of work – rest duration on intermittent exercise and subsequent performance

This study examined the effects of different work?–?rest durations during 40?min intermittent treadmill exercise and subsequent running performance. Eight males (mean?±?s: age 24.3?±?2.0 years, body mass 79.4?±?7.0?kg, height 1.77?±?0.05?m) undertook intermittent exercise involving repeated sprints at 120% of the speed at which maximal oxygen uptake (v-[Vdot]O_2max) was attained with passive recovery between each one. The work?–?rest ratio was constant at 1:1.5 with trials involving short (6:9?s), medium (12:18?s) or long (24:36?s) work?–?rest durations. Each trial was followed by a performance run to volitional exhaustion at 150% v-[Vdot]O_2max. After 40?min, mean exercise intensity was greater during the long (68.4?±?9.3%) than the short work?–?rest trial (54.9?±?8.1% [Vdot]O_2max; P?<?0.05). Blood lactate concentration at 10?min was higher in the long and medium than in the short work?–?rest trial (6.1?±?0.8, 5.2?±?0.9, 4.5?±?1.3?mmol?·?l^?1, respectively; P?<?0.05). The respiratory exchange ratio was consistently higher during the long than during the medium and short work?–?rest trials (P <?0.05). Plasma glucose concentration was higher in the long and medium than in the short work?–?rest trial after 40?min of exercise (5.6?±?0.1, 6.6?±?0.2 and 5.3?±?0.5?mmol?·?l^?1, respectively; P?<?0.05). No differences were observed between trials for performance time (72.7?±?14.9, 63.2?±?13.2, 57.6?±?13.5?s for the short, medium and long work?–?rest trial, respectively; P = 0.17), although a relationship between performance time and 40?min plasma glucose was observed (P?<?0.05). The results show that 40?min of intermittent exercise involving long and medium work?–?rest durations elicits greater physiological strain and carbohydrate utilization than the same amount of intermittent exercise undertaken with a short work?–?rest duration.     

Effect of biological maturation on maximal oxygen uptake and ventilatory thresholds in soccer players: An allometric approach

Abstract

In this study, we investigated the effect of biological maturation on maximal oxygen uptake ([Vdot]O_2max) and ventilatory thresholds (VT₁ and VT₂) in 110 young soccer players separated into pubescent and post-pubescent groups.. Maximal oxygen uptake and [Vdot]O₂ corresponding to VT₁ and VT₂ were expressed as absolute values, ratio standards, theoretical exponents, and experimentally observed exponents. Absolute [Vdot]O₂ (ml · min^?1) was different between groups for VT₁, VT₂, and [Vdot]O_2max. Ratio standards (ml · kg^?1 · min^?1) were not significantly different between groups for VT₁, VT₂, and [Vdot]O_2max. Theoretical exponents (ml · kg^?0.67 · min^?1 and ml · kg^?0.75 · min^?1) were not properly adjusted for the body mass effects on VT₁, VT₂, and [Vdot]O_2max. When the data were correctly adjusted using experimentally observed exponents, VT₁ (ml · kg^?0.94 · min^?1) and VT₂ (ml · kg^?0.95 · min^?1) were not different between groups. The experimentally observed exponent for [Vdot]O_2max (ml · kg^?0.90 · min^?1) was different between groups (P = 0.048); however, this difference could not be attributed to biological maturation. In conclusion, biological maturation had no effect on VT₁, VT₂ or [Vdot]O_2max when the effect of body mass was adjusted by experimentally observed exponents. Thus, when evaluating the physiological performance of young soccer players, allometric scaling needs to be taken into account instead of using theoretical approaches.     

A modified TRIMP to quantify the in-season training load of team sport players

Abstract

The aims of the study were to modify the training impulse (TRIMP) method of quantifying training load for use with intermittent team sports, and to examine the relationship between this modified TRIMP (TRIMP_MOD) and changes in the physiological profile of team sport players during a competitive season. Eight male field hockey players, participating in the English Premier Division, took part in the study (mean±s: age 26±4 years, body mass 80.8±5.2 kg, stature 1.82±0.04 m). Participants performed three treadmill exercise tests at the start of the competitive season and mid-season: a submaximal test to establish the treadmill speed at a blood lactate concentration of 4 mmol · l^?1; a maximal incremental test to determine maximal oxygen uptake ([Vdot]O_2max) and peak running speed; and an all-out constant-load test to determine time to exhaustion. Heart rate was recorded during all training sessions and match-play, from which TRIMP_MOD was calculated. Mean weekly TRIMP_MOD was correlated with the change in [Vdot]O_2max and treadmill speed at a blood lactate concentration of 4 mmol · l^?1 from the start of to mid-season (P<0.05). The results suggest that TRIMP_MOD is a means of quantifying training load in team sports and can be used to prescribe training for the maintenance or improvement of aerobic fitness during the competitive season.     

The respiratory time and flow profile at volitional exercise termination

Abstract

In this study, we examine the effect of exercise on the time and flow characteristics of the respiratory cycle profile at the point of volitional exercise termination. Eight males (mean age 29 years, s = 10; body mass 74 kg, s = 7; height 1.75 m, s = 0.04) undertook a cycle test to volitional exhaustion on a cycle ergometer, which allowed peak oxygen uptake ([Vdot]O_2peak) to be measured (mean 51 ml · kg^?1 · min^?1, s = 7). At a later date, two sub-maximal tests to volitional exhaustion were completed in a random order at 76% (s = 6) and 86%[Vdot]O_2peak (s = 7). As expected, the magnitude of the respiratory flow and time characteristics varied with the three exercise intensities, as did the point of exercise termination and terminal ventilation rates, which varied from 7 to 27 min and 112 to 132 litres · min^?1 respectively. More importantly, however, at exercise termination some of the characteristics were similar, particularly the breathing frequency (at termination 49 breaths · min^?1), the ratio between inspiration and total breath time (0.5), and the later occurrence of peak inspiratory flow (0.24 – 0.48 s). The coincident unity of these time and flow profile characteristics at exercise termination illustrates how the integration of timing and flow during breathing influence exercise capacity in non-elite athletes.     

Effects of exercise intensity on salivary antimicrobial proteins and markers of stress in active men

Abstract

In the present study, we assessed the effects of exercise intensity on salivary immunoglobulin A (s-IgA) and salivary lysozyme (s-Lys) and examined how these responses were associated with salivary markers of adrenal activation. Using a randomized design, 10 healthy active men participated in three experimental cycling trials: 50% maximal oxygen uptake ([Vdot]O_2max), 75%[Vdot]O_2max, and an incremental test to exhaustion. The durations of the trials were the same as for a preliminary incremental test to exhaustion (22.3 min, s _x  = 0.8). Timed, unstimulated saliva samples were collected before exercise, immediately after exercise, and 1 h after exercise. In the incremental exhaustion trial, the secretion rates of both s-IgA and s-Lys were increased. An increase in s-Lys secretion rate was also observed at 75%[Vdot]O_2max. No significant changes in saliva flow rate were observed in any trial. Cycling at 75%[Vdot]O_2max and to exhaustion increased the secretion of α-amylase and chromogranin A immediately after exercise; higher cortisol values at 75%[Vdot]O_2max and in the incremental exhaustion trial compared with 50%[Vdot]O_2max were observed 1 h immediately after exercise only. These findings suggest that short-duration, high-intensity exercise increases the secretion rate of s-IgA and s-Lys despite no change in the saliva flow rate. These effects appear to be associated with changes in sympathetic activity and not the hypothalamic – pituitary – adrenal axis.     

Carbohydrate ingestion and soccer skill performance during prolonged intermittent exercise

Abstract

The aim of this study was to investigate the effect of ingesting a carbohydrate-electrolyte solution, during the 90-min Loughborough Intermittent Shuttle Test, on soccer skill performance. Seventeen male soccer players ingested either a 6.4% carbohydrate-electrolyte solution or placebo solution equivalent to 8 ml · kg^?1 body mass before exercise and 3 ml · kg^?1 body mass after every 15 min of exercise, in a double-blind randomized cross-over design, with the trials separated by 7 days. The evening before the main trial, the participants performed glycogen-reducing exercise on a cycle ergometer (80 min at 70%[Vdot]O_2max) and were then fed a low-carbohydrate meal. After a 12-h overnight fast, they performed The Loughborough Soccer Passing Test before and after every 15 min of exercise. Analysis of the combined skill test data showed a significant time effect (P = 0.001) with differences between 0–45 and 75–90 min (P < 0.05). There was a 3% reduction in skill performance from before to after exercise in the carbohydrate-electrolyte trial, whereas in the placebo trial the decrease was 14% (P = 0.07). In conclusion, skill performance during the simulated soccer activity appeared to deteriorate in the last 15–30 min of exercise. However, providing 52 g · h^?1 carbohydrate during exercise showed a tendency to better maintain soccer skill performance than a taste-matched placebo.     

The multi-stage fitness test as a predictor of endurance fitness in wheelchair athletes

Abstract

The aims of this study were two-fold: (1) to consider the criterion-related validity of the multi-stage fitness test (MSFT) by comparing the predicted maximal oxygen uptake ([Vdot]O_2max) and distance travelled with peak oxygen uptake ([Vdot]O_2peak) measured using a wheelchair ergometer (n = 24); and (2) to assess the reliability of the MSFT in a sub-sample of wheelchair athletes (n = 10) measured on two occasions. Twenty-four trained male wheelchair basketball players (mean age 29 years, s = 6) took part in the study. All participants performed a continuous incremental wheelchair ergometer test to volitional exhaustion to determine [Vdot]O_2peak, and the MSFT on an indoor wooden basketball court. Mean ergometer [Vdot]O_2peak was 2.66 litres · min^?1 (s = 0.49) and peak heart rate was 188 beats · min^?1 (s = 10). The group mean MSFT distance travelled was 2056 m (s = 272) and mean peak heart rate was 186 beats · min^?1 (s = 11). Low to moderate correlations (ρ = 0.39 to 0.58; 95% confidence interval [CI]: ?0.02 to 0.69 and 0.23 to 0.80) were found between distance travelled in the MSFT and different expressions of wheelchair ergometer [Vdot]O_2peak. There was a mean bias of ?1.9 beats · min^?1 (95% CI: ?5.9 to 2.0) and standard error of measurement of 6.6 beats · min^?1 (95% CI: 5.4 to 8.8) between the ergometer and MSFT peak heart rates. A similar comparison of ergometer and predicted MSFT [Vdot]O_2peak values revealed a large mean systematic bias of 15.3 ml · kg^?1 · min^?1 (95% CI: 13.2 to 17.4) and standard error of measurement of 3.5 ml · kg^?1 · min^?1 (95% CI: 2.8 to 4.6). Small standard errors of measurement for MSFT distance travelled (86 m; 95% CI: 59 to 157) and MSFT peak heart rate (2.4 beats · min^?1; 95% CI: 1.7 to 4.5) suggest that these variables can be measured reliably. The results suggest that the multi-stage fitness test provides reliable data with this population, but does not fully reflect the aerobic capacity of wheelchair athletes directly.