The influence of carbohydrate and protein ingestion during recovery from prolonged exercise on subsequent endurance performance

Abstract

Ingesting carbohydrate plus protein following prolonged exercise may restore exercise capacity more effectively than ingestion of carbohydrate alone. The objective of the present study was to determine whether this potential benefit is a consequence of the protein fraction per se or simply due to the additional energy it provides. Six active males participated in three trials, each involving a 90-min treadmill run at 70% maximal oxygen uptake (run 1) followed by a 4-h recovery. At 30-min intervals during recovery, participants ingested solutions containing: (1) 0.8 g carbohydrate · kg body mass (BM)^?1 · h^?1 plus 0.3 g · kg^?1 · h^?1 of whey protein isolate (CHO-PRO); (2) 0.8 g carbohydrate · kg BM^?1 · h^?1 (CHO); or (3) 1.1 g carbohydrate · kg BM^?1 · h^?1 (CHO-CHO). The latter two solutions matched the CHO-PRO solution for carbohydrate and for energy, respectively. Following recovery, participants ran to exhaustion at 70% maximal oxygen uptake (run 2). Exercise capacity during run 2 was greater following ingestion of CHO-PRO and CHO-CHO than following ingestion of CHO (P ≤ 0.05) with no significant difference between the CHO-PRO and CHO-CHO treatments. In conclusion, increasing the energy content of these recovery solutions extended run time to exhaustion, irrespective of whether the additional energy originated from sucrose or whey protein isolate.     

Urea production during prolonged swimming

Male interscholastic swimmers (n = 8) completed a 4572 m training swim in 62 ±1.1 min (x ± s.e.) with terminal heart rate and blood lactate of 152 ± 6 beats min^‐1 and 6.9±0.89 mM, respectively. Sweat rate (0.48±0.0951. h^‐1) was lower than similar intensity cycling (1.5±0.13 1. h^‐1) or running (1.1 ± 0.14 l.h^‐1). Post‐swim serum urea N (11.6±0.71 mM) was elevated (P<0.05) vs pre‐swim (4.6±0.39 mM). Post‐swim urine volume (860±75 ml 24 h^‐1) was reduced (P<0.07) and resulted in an elevated (P<0.05), but delayed (24–84 h), post‐exercise urea N excretion. Although the reduced urine and sweat production during the swim undoubtedly contributed to the elevated serum urea, there must be another explanation because together they could only account for 38% of the observed increase. On the basis of the magnitude of serum urea increase, it appears that the swim caused an increase in urea production (amino acid oxidation). The failure to observe larger increases in urinary urea during recovery indicates that either urea excretion following exercise continues for prolonged periods of time (>48 h) or another significant mode of nitrogen excretion exists.     

Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans

Abstract

The synergistic stimulating effect of combined intake of carbohydrate and protein on plasma insulin concentration has been reported previously. However, it remains unclear whether the amount of protein ingested after exercise affects the concentrations of plasma insulin and amino acids. This study of trained men compared the effects of post-exercise co-ingestion of carbohydrate plus different amounts of whey protein hydrolysates (WPHs) with carbohydrate alone on (1) blood biochemical parameters of carbohydrate metabolism during the post-exercise phase, and (2) endurance performance. Eight trained men exercised continuously for 70 min. Immediately after exercise and 30, 60, 90, and 120 min later, the participants received supplements containing: (1) 17.5 g carbohydrate, (2) 3.0 g WPHs and 17.5 g carbohydrate (L-WPH), or (3) 8.0 g WPHs and 17.5 g carbohydrate (H-WPH). After a 2-h recovery period, the participants performed an endurance performance test. The concentrations of blood glucose were lower and plasma insulin significantly higher in the H-WPH trial compared with the carbohydrate trial. The concentrations of plasma amino acids were increased in a dose-dependent manner following ingestion of different amounts of WPHs with carbohydrate. Endurance performance was not significantly different between the three trials. Co-ingestion of carbohydrate and H-WPH was more effective than ingestion of carbohydrate alone for stimulating insulin secretion and increasing the availability of plasma amino acids. These results suggest that plasma concentrations of amino acids during the recovery period are determined by the amount of dietary protein ingested, and that it is necessary to increase the concentration of plasma amino acids above a certain level to stimulate insulin secretion.     

The European College of Sports Sciences Position statement: The role of stretching exercises in sports

Abstract

Possible peripheral mechanisms of fatigue have been widely documented, including the depletion of muscle glycogen and the loss of body fluids. The notion that the brain may be intimately involved in the fatigue process is not a new one, but recently possible neurobiological mechanisms involved in this response have been investigated. Changes in central neurotransmission occur during exercise that may result in feelings of tiredness, lethargy, and a loss of motivation to continue exercise, contributing to the development of fatigue. There is evidence that manipulation of the neurotransmitters serotonin, dopamine, and noradrenaline, through the administration of pharmacological agents, may delay the onset of fatigue during prolonged exercise, particularly when performing in a warm environment. Supplementation with branched-chain amino acids and tyrosine can influence perceived exertion and some measures of mental performance, but the results of several apparently well-controlled laboratory studies have not demonstrated a positive effect on exercise capacity under temperate conditions. The ergogenic effects of carbohydrate and caffeine are well documented, but often little attention is paid to the central effects of these nutrients. Carbohydrate ingestion has been demonstrated to alter brain activity and cerebral metabolism, factors that may be important in the development of fatigue and the maintenance of skill performance. There is strong evidence for a positive effect of caffeine on exercise performance, with recent data highlighting the role of central adenosine as a mediator of this response.     

运动性疲劳概述

一个世纪以来，运动性疲劳一直是体育科学研究中重要的课题，本文旨在回顾过去，展望未来，对有关运动性疲劳的研究：疲劳的概念及其分类、疲劳的产生机制与学说、疲劳的测定与判断、疲劳的恢复与预防等做了较全面的概述。     

Selected physiological and perceptual responses during a simulated limited overs century in non-elite batsmen

Few studies have examined the impact of an increased physical demand on batting performance, especially over extended periods of play. Therefore, the purpose of this study was to determine the physiological and perceptual responses of batsmen scoring a simulated limited overs century, and to link these to sprint times and accuracy of the impact of the ball on the bat. Seventeen male, university level cricketers, performed a batting protocol (BATEX^©), typical of a limited overs century. The protocol consisted of six stages, each of five overs, with each stage matched to a specific phase of play. Throughout the protocol heart rate (HR), central ratings of perceived effort (RPE), sprint times and impact accuracy were recorded. HR fluctuated as a function of exercise intensity (124.16–159.61?bpm). Central RPE increased as a function of intensity and duration (11.87–16.04). Sprint times got slower over time (5.67–5.81?s), while impact accuracy improved significantly (p?.05) after stage one and then plateaued for the remainder of the protocol (64.81–57.39?mm). In conclusion, the protocol significantly impacted cardiac strain and perceptual responses negatively impacting sprint times with an improvement in batting accuracy.     

Effects of carbohydrate ingestion on skill maintenance in squash players

Abstract

The effects of carbohydrate (CHO) ingestion during sports which require high levels of motor and cognitive skill, such as squash, have produced conflicting results. This study aimed to explore the effect of CHO ingestion on squash skill following short duration exercise simulating the demands of squash play. Sixteen male squash players of a high standard were recruited. Following a VO₂max test, and familiarisation trial, subjects completed two further trials assessing skill pre- and post-exercise designed to simulate the demands of squash play. A squash skill test assessed accuracy of the forehand and backhand straight drives. Exercise consisted of 20 minutes of shuttle running at 82(±5)% HR_max, and 9 minutes of ghosting at 94(±4)% HR_max. Capillary blood samples (20 µl) were taken at five intervals for measurement of glucose and lactate. Cognitive function was measured with choice visual and auditory reaction time (RT) tests pre- and post-exercise, as was forearm wrist flexor MVC and fatigue profile. CHO drink (6.4% CHO) or matched placebo (PL) were administered after the initial skill test (500 ml), after the shuttle running (250 ml), and after the ghosting (250 ml) in a double blind crossover design. There was no overall effect of CHO ingestion on skill maintenance (p=0.10) however, significantly fewer balls landed outside the scoring zone (p=0.03) on the CHO ingestion trial. There was no change of visual RT pre- to post-exercise on PL (+0.01±0.03s), but a significant improvement (?0.07±0.05s) was observed in the CHO trial. Auditory RT improved pre- to post-exercise during both trials. MVC and fatigue profile of the wrist flexors was not different between trials but showed a force decrement pre- to post-exercise (p<0.05). A significant difference in blood glucose was observed between trials (p<0.01) but blood lactate response during both trials was similar. These results lend some support to a beneficial effect of CHO ingestion on skill during game sports.     

Nutritional needs of elite endurance athletes. Part II: Dietary protein and the potential role of caffeine and creatine

Abstract

Amino acids contribute between 2–8% of the energy needs during endurance exercise. Endurance exercise training leads to an adaptive reduction in the oxidation of amino acids at the same absolute exercise intensity, however, the capacity to oxidize amino acids goes up due to the increase in the total amount of the rate limiting enzyme, branched chain 2-oxo-acid dehydrogenase. There appears to be a modest increase (range?=?12–95%) in protein requirements only for very well trained athletes who are actively training. Although the majority of athletes will have ample dietary protein to meet any increased need, those on a hypoenergetic diet or during extreme periods of physical stress may need dietary manipulation to accommodate the need. Caffeine is a trimethylxanthine derivative that is common in many foods and beverages. The consumption of caffeine (3–7 mg/kg) prior to endurance exercise improves performance for habitual and non-habitual consumers. The ergogenic effect is likely due to a direct effect on muscle contractility and not via an enhancement of fatty acid oxidation. Creatine is important in intra-cellular energy shuttling and in cellular fluid regulation. Creatine monohydrate supplementation (20 g/d X 3–5 days) increases fat-free mass, improves muscle strength during repetitive high intensity contractions and increases fat-free mass accumulation and strength during a period of weight training. Given the increase in weight, there are likely neutral or even performance reducing effects in sports that are influenced by body mass (i.e., running, hill climbing cycling).     

补充不同剂量支链氨基酸对运动大鼠下丘脑5-羟色胺及其前体的影响

观察补充支链氨基酸(BCAA)后大鼠力竭游泳运动恢复期下丘脑细胞外液BCAA、游离色氨酸(f-Trp)和5-羟色胺(5-HT)的变化,分析补充BCAA是否能够提高大鼠的运动能力,比较补充BCAA的效果,探索补充BCAA的最佳剂量。方法:将32只大鼠随机分为对照组、小剂量补充组[1.25g/(kg·BW-1)]、中剂量补充组[2.5g/kg·BW-1)]和大剂量补充组[5g/(kg·BW-1)],每组8只。BCAA补充方案为:每天上午补充一次,每周6次(周日暂停),持续6周。6周后,在大鼠进行力竭游泳运动前一天下午取大鼠尾血测试血氨。采用毛细管电泳-激光诱导荧光法检测大鼠下丘脑细胞外液BCAA、f-Trp和5-HT的浓度。结论:血氨随着BCAA补充剂量的增加而升高,小剂量补充BCAA对机体血氨的生成无影响;补充BCAA能提高大鼠力竭游泳运动能力,但并不随BCAA补充剂量的增加而提高;补充BCAA可能提高了血浆BCAA与f-Trp竞争通过血脑屏障的能力且具有推迟和减少脑5-HT生成的作用;小剂量[1.25g/(kg·BW-1)]补充BCAA对提高大鼠运动能力效果最佳。     

运动员补糖、支链氨基酸和乳清蛋白的临床应用观察

目的：观察补充糖饮料、支链氨基酸和乳清蛋白等能量物质对阶段性大运动量训练维持或提高运动员身体机能和疲劳恢复的影响。方法：根据冬训阶段性训练计划安排,对10名散打运动员在30d的体能训练阶段,按营养补充方案进行能量补充,观察能量补充对机体无氧能力的输出总功、最大功率、疲劳指数,有氧能力的拉桨时间、功率、桨频,生化指标血乳酸（LA）尿素氮（BUN）、血糖（GL）、肌酸激酶（CK）的影响,另选10名队友作空白组,进行自身前后对照和空白对照。结果：观察组经过阶段性能量补充前后运动员的输出总功、最大功率、疲劳指数和桨频均显著提高（P〈0．01）,与空白组相比也有明显提高（P〈0．05）,训练课后血糖水平也明显提高（P〈0．05）,补充支链氨基酸和乳清蛋白后血BUN的恢复与空白组比有极显著好转（P〈0．001）。结论：训练期间补充糖、支链氨基酸和乳清蛋白等能量物质,能明显改善运动员的有氧运动能力和无氧运动能力,明显延缓运动性疲劳的发生,加速运动性疲劳恢复的作用。     

射箭运动员在不同训练时期血液氨基酸变化的研究

目的：观察射箭运动员在不同运动负荷状态时的血液氨基酸的变化。方法：用高效液相法测定射箭运动员冬训期间不同的训练时期血液中四种氨基酸——门冬氨酸（Asp）、谷氨酸（Glu）、甘氨酸（（Gly）和r-氨基丁酸（GABA）的含量。结果：在进行中等强度训练期和大强度训练期运动后1h血浆中谷氨酸的血浆浓度明显升高（P〈0．01）。而在大强度训练期后明显下降,甚至低于安静值;门冬氨酸在中等强度训练后血浆浓度也升高（P〈0．05）,在疲劳期则呈下降趋势,但仍高于安静值;在进行中等强度训练期和大强度训练期运动1h后血浆中r-氨基丁酸、甘氨酸均上升。大强度训练期明显升高,与训练前期安静值相比有显著意义（P〈0．01）。结论：射箭运动员在长时间大强度大运动量训练后,血液中的氨基酸会发生变化,中枢疲劳可能从血浆氨基酸的变化反映出来。     

The effects of short versus long inter-set rest intervals in resistance training on measures of muscle hypertrophy: A systematic review

Although the effects of short versus long inter-set rest intervals in resistance training on measures of muscle hypertrophy have been investigated in several studies, the findings are equivocal and the practical implications remain unclear. In an attempt to provide clarity on the topic, we performed a systematic literature search of PubMed/MEDLINE, Scopus, Web of Science, Cochrane Library, and Physiotherapy Evidence Database (PEDro) electronic databases. Six studies were found to have met the inclusion criteria: (a) an experimental trial published in an English-language peer-reviewed journal; (b) the study compared the use of short (≤60?s) to long (>60?s) inter-set rest intervals in a traditional dynamic resistance exercise using both concentric and eccentric muscle actions, with the only difference in resistance training among groups being the inter-set rest interval duration; (c) at least one method of measuring changes in muscle mass was used in the study; (d) the study lasted for a minimum of four weeks, employed a training frequency of ≥2 resistance training days per week, and (e) used human participants without known chronic disease or injury. Current evidence indicates that both short and long inter-set rest intervals may be useful when training for achieving gains in muscle hypertrophy. Novel findings involving trained participants using measures sensitive to detect changes in muscle hypertrophy suggest a possible advantage for the use of long rest intervals to elicit hypertrophic effects. However, due to the paucity of studies with similar designs, further research is needed to provide a clear differentiation between these two approaches.     

The 3-minute all-out cycling test is sensitive to changes in cadence using the Lode Excalibur Sport ergometer

This study investigated the effect cadence has on the estimation of critical power (CP) and the finite work capacity (W?) during the 3-minute all-out cycling test. Ten participants completed 8 tests: 1) an incremental test to calculate gas exchange threshold (GET), maximal aerobic power (MAP) and peak oxygen uptake (V?O_2peak), 2–4) three time-to-exhaustion tests at 80, 100 and 105% MAP to calculate CP and W?, 5–7) four 3-minute all-out tests to calculate end power (EP) and work done above EP (WEP) using cadences ranging from preferred ?5 to preferred +10 rev·min^?1 to set the fixed resistance. Significant differences were seen between CP and EP-preferred (267.5 ± 22.6 W vs. 296.6 ± 26.1 W, P < 0.001), CP and EP?5 (267.5 ± 22.6 W vs. 303.6 ± 24.0 W, P < 0.001) and between CP and EP+5 (267.5 ± 22.6 W vs. 290.0 ± 28.0 W, P = 0.002). No significant differences were seen between CP and EP+10 (267.5 ± 22.6 W vs. 278.1 ± 30.9 W, P = 0.331). Significant differences were seen between W? and WEP at all tested fixed resistances. EP is reduced when cycling at higher than preferred cadences, providing better estimates of CP.     

射箭运动员不同训练时期血液内分泌和氨基酸变化的研究

目的:观察射箭运动员在冬训期间不同训练时期的血液内分泌指标和氨基酸的变化。方法:用化学发光法测定运动员血清睾酮(T)和皮质醇(C),用高效液相色谱法测定血液中四种氨基酸天门冬氨酸(Asp)、谷氨酸(Glu)、甘氨酸(Gly)和r-氨基丁酸(GABA)的含量。结果:男运动员训练疲劳后血睾酮明显下降(P<0.01),与进行中等强度训练时比较也降低,具有显著性意义(P<0.05)。运动员在运动疲劳期,皮质醇显著升高(P<0.01),而T/C比值显著下降(P<0.01)。女运动员在大强度大运动量训练后,血睾酮、T/C比值明显下降(P<0.05),皮质醇明显升高。中等强度训练时,男、女皮质醇较训练前期明显升高(P<0.05)。在进行中等强度训练期和大强度训练期运动后1小时血浆中谷氨酸的血浆浓度明显升高(P<0.01),而在大强度训练期后明显下降,低于安静值。门冬氨酸在中等强度训练后血浆浓度也升高(P<0.05),在疲劳期则呈下降趋势,但仍高于安静值。在进行中等强度训练期和大强度训练期运动1小时后血浆中r-氨基丁酸、甘氨酸均上升,大强度训练期明显升高,与训练前期安静值相比有显著意义(P<0.01)。结论:射箭运动员在长时...     

运动与补糖研究进展

随着近代体育运动竞技水平的提高,教练员与运动员已开始认识到补糖与提高运动成绩之间关系的重要性。通过补糖,不仅可以提高运动成绩,而且可以推延运动性疲劳的出现,运动后补糖还可以促进运动性疲劳的恢复。近代关于补糖与运动的研究中,涉及了补充不同种类的糖(复合糖、低聚糖、1、6一二磷酸果糖等)与各种类型运动项目及糖的补充时间之间的关系,本文将对这些可行性研究进行分析讨论以供教练员和运动员参考。     

碳水化合物对高强度折返运动能力的影响

运动对人体代谢的需要是代表一种营养上的挑战。营养能提供体内能量的再补充、生长、修复和水份的补充。运动期间能量代谢，碳水化合物扮演着重要的角色。虽然许多研究报告表明运动中补充碳水化合物可提高持续亚极限运动的耐力，但有些项目如足球、篮球等运动移位的方式，包括了高低速度折返跑和冲刺运动。本文研究的目的是了解碳水化合物对折返能力的影响     

The influence of isometric preload on power expressed during bench press in strength-trained men

The purpose of this study was to compare the power expressed during the bench press exercise in resistance-trained men following different pre-activation conditions. Twenty-two trained men (age 24.1?±?1.7 years, height 178.6?±?6.1?cm, body mass 81.1?±?10.6?kg) completed a maximal effort bench press (1-RM) test (100.0?kg?±?8.1?kg). In a subsequent assessment, each participant performed concentric bench press movements with loads of 20%, 30%, 40% and 50% of their 1-RM preceded by either a concentric contraction (CC), a low isometric preload (LIP; 70% 1-RM) or a high isometric preload (HIP; 100% 1-RM) conditions. All movements were performed in a Smith machine with a settable quick-release device. Participants performed all three conditions in randomized fashion. Results indicated that power outputs during the bench press exercise following HIP were significantly (p?<?0.05) greater than CC at 20% 1-RM (+9%), 30% 1-RM (+16%) and 40% 1-RM (+14%), and LIP at 20% 1-RM (+4%), 30% 1-RM (+20%) and 40% 1-RM (+15%). No differences were found between conditions at 50% 1-RM. Area under the force–power curve with HIP was greater (p?<?0.05) than with CC and LIP. In conclusion, results of this study indicate that the use of a HIP (100% 1-RM) in trained participants results in significantly greater power output during the concentric phase of a multi-joint exercise when compared to standard concentric movement.     

Reproducibility of measurement techniques used for creatine kinase,interleukin-6 and high-sensitivity C-reactive protein determination over a 48 h period in males and females

To examine the reproducibility of three measurement techniques used to determine creatine kinase, interleukin-6 and high-sensitivity C-reactive protein, 50 participants had blood samples taken on two occasions. Fingertip plasma samples were analysed using the Reflotron for CK determination. Venous blood samples collected into serum separator tubes were used for IL-6 and hs-CRP analyses. IL-6 was measured using an enzyme linked immune assay development kit. The hs-CRP was measured by an in-house ELISA method. Dependent t-tests showed no systematic bias between samples. The interdian CV was 20.0% for CK, 15.3% for IL-6 and 44.2% for hs-CRP. The intraclass correlation coefficient was 0.90 for CK, 0.98 for IL-6 and 0.70 for hs-CRP. The 95% limits of agreement were ?69.7 to 63.5 IU/L for CK, ?1.48 to 1.80 pg/ml for IL-6 and ?1.10 to 0.91 µg/L for hs-CRP. The results demonstrate low absolute reproducibility, which may obscure a true experimental effect.     

Carbohydrate gel ingestion during running in the heat on markers of gastrointestinal distress

The purpose of this study is to measure the effects of carbohydrate ingestion during exercise in the heat by measuring markers of gastrointestinal damage and inflammation. Methods: Active subjects (n?=?7) completed two 60-min running trials in a heated environment (70% VO₂max, 30°C). At minute 20 of exercise, subjects consumed a carbohydrate gel (Cho) (27?g), or a non-carbohydrate placebo (nCho). Plasma endotoxin, I-FABP, TNF-α, IL-6, IL-1β, IL-10, and MCP-1 were measured pre-exercise, 20-min post-exercise, and again 2-h, and 4-h post-exercise. Results: Endotoxin increased 20-min post-exercise compared to pre in the Cho trial only (p?=?.03). I-FABP levels increased 20-min post-exercise in the Cho trial only compared to pre-exercise (p?=?.003). I-FABP levels were also increased in Cho trial 20-min post-exercise when compared to same time point in the nCho trial (p?=?.032). TNF-α increased 20-min post-exercise in the Cho trial only compared to pre (p?=?.03). Plasma IL-6 concentration increased 20-min post-exercise when compared to pre in both the Cho (p?=?.002) and nCho (p?=?.009), but remained elevated at the 2-h time point in the nCho trial (p?=?.03). I-FABP and several plasma cytokines (TNF-α, MCP-1, Il-6) returned to baseline sooner in the Cho trial. Conclusions: Ingestion of carbohydrate gel during exercise in the heat enhances markers of gastrointestinal wall damage.