Continuous walking and time- and intensity-matched interval walking: Cardiometabolic demand and post-exercise enjoyment in insufficiently active,healthy adults

ABSTRACT

We compared cardiometabolic demand and post-exercise enjoyment between continuous walking (CW) and time- and intensity-matched interval walking (IW) in insufficiently active adults. Sixteen individuals (13 females and three males, age 25.3 ± 11.1 years) completed one CW and one IW session lasting 30 min in a randomised-counterbalanced design. For CW, participants walked at a mean intensity of 65–70% predicted maximum heart rate (HR_max). For IW, participants alternated between 3 min at 80% HR_max and 2 min at 50% HR_max. Expired gas was measured throughout each protocol. Participants rated post-exercise enjoyment following each protocol. Mean HR and V˙O₂ showed small positive differences in IW vs. CW (2, 95%CL 0, 4 beat.min^?1; d = 0.23, 95%CL 0.06, 0.41 and 1.4, 95%CL 1.2 ml.kg^?1.min^?1, d = 0.36, 95%CL 0.05, 0.65, respectively). There was a medium positive difference in overall kcal expenditure in IW vs. CW (25, 95%CL 7 kcal, d = 0.58, 95%CL 0.33, 0.82). Post-exercise enjoyment was moderately greater following IW vs. CW (9.1, 95%CL 1.4, 16.8 AU, d = 0.62, 95%CL 0.06, 0.90), with 75% of participants reporting IW as more enjoyable. Interval walking elicits meaningfully greater energy expenditure and is more enjoyable than CW in insufficiently active, healthy adults.     

The effect of induced alkalosis and submaximal cycling on neuromuscular response during sustained isometric contraction

Abstract

The aim of this study was to determine if inducing metabolic alkalosis would alter neuromuscular control after 50 min of standardized submaximal cycling. Eight trained male cyclists (mean age 32 years, s = 7; [Vdot]O_2max 62 ml · kg^?1 · min^?1, s = 8) ingested capsules containing either CaCO₃ (placebo) or NaHCO₃ (0.3 g · kg^?1 body mass) in eight doses over 2 h on two separate occasions, commencing 3 h before exercise. Participants performed three maximal isometric voluntary contractions (MVC) of the knee extensors while determining the central activation ratio by superimposing electrical stimulation both pre-ingestion and post-exercise, followed by a 50-s sustained maximal contraction in which force, EMG amplitude, and muscle fibre conduction velocity were assessed. Plasma pH, blood base excess, and plasma HCO₃ were higher (P < 0.01) during the NaHCO₃ trial. After cycling, muscle fibre conduction velocity was higher (P < 0.05) during the 50-s sustained maximal contraction with NaHCO₃ than with placebo (5.1 m · s^?1, s = 0.4 vs. 4.2 m · s^?1, s = 0.4) while the EMG amplitude remained the same. Force decline rate was less (P < 0.05) during alkalosis-sustained maximal contraction and no differences were shown in central activation ratio. These data indicate that induced metabolic alkalosis can increase muscle fibre conduction velocity following prolonged submaximal cycling.     

Muscle oxygenation and EMG activity during isometric exercise in children

Abstract

The aim of this study was to examine the time-course of and the relationships between muscle oxygenation, blood volume and myoelectrical manifestations during isometric exercise in children. Twelve healthy children aged 12.5 ± 1.2 years (mean ± s) performed an isometric knee extension at 50% of their maximal voluntary contraction (MVC) until exhaustion to assess endurance time (limit time, T _lim). Changes in muscle oxygenation and blood volume were assessed by near infrared spectroscopy (NIRS). The root mean square (RMS) amplitude and the mean power frequency (MPF) from electromyogram (EMG) signals were obtained, as NIRS parameters, from the vastus lateralis. Mean T _lim was 117 ± 34 s. The muscle oxygenation and blood volume curves decreased immediately at the beginning of exercise. Maximal deoxygenation occurred at 50% T _lim, and fell by 76.9% from the resting value. Similarly, minimal blood volume was observed at 50% T _lim; it reached a plateau that lasted until the end of exercise. The kinetics of the MPF and RMS curves were inversely related to time. At the last set of exercises (after 75% T _lim), a steeper RMS curve and an abrupt decrease in the MPF curve were observed. Significant correlations (r) between muscle oxygenation, blood volume, root mean square amplitude and mean power frequency were observed, which ranged from 0.72 to 0.99. These findings suggest that the fatigue resulting from sustained isometric exercise is related to a decrease in oxygenation and blood volume.     

The effects of sodium bicarbonate (NaHCO3) ingestion on high intensity cycling capacity

Abstract

Ten healthy, non-cycling trained males (age: 21.2 ± 2.2 years, body mass: 75.9 ± 13.4 kg, height: 178 ± 6 cm, [Vdot]O_2PEAK: 46 ± 10 ml · kg^?1 · min^?1) performed a graded incremental exercise test, two familiarisation trials and six experimental trials. Experimental trials consisted of cycling to volitional exhaustion at 100%, 110% and 120% W_PEAK, 60 min after ingesting either 0.3 g · kg^?1 body mass sodium bicarbonate (NaHCO₃) or 0.1 g · kg^?1 body mass sodium chloride (placebo). NaHCO₃ ingestion increased cycling capacity by 17% at 100% W_PEAK (327 vs. 383 s; P = 0.02) although not at 110% W_PEAK (249 vs. 254 s; P = 0.66) or 120% W_PEAK (170 vs. 175 s; P = 0.60; placebo and NaHCO₃ respectively). Heart rate (P = 0.02), blood lactate (P = 0.001), pH (P < 0.001), [HCO₃ ^?], (P < 0.001), and base excess (P < 0.001) were greater in all NaHCO₃ trials. NaHCO₃ attenuated localised ratings of perceived exertion (RPE_L) to a greater extent than placebo only at 100% W_PEAK (P < 0.02). Ratings of abdominal discomfort and gut fullness were mild but higher for NaHCO₃. NaHCO₃ ingestion significantly improves continuous constant load cycling at 100% W_PEAK due to, in part, attenuation of RPE_L.     

Effects of an acute bout of exercise on serum soluble leptin receptor (sOB-R) levels

Abstract

We investigated the effects of an acute bout of exercise on serum soluble leptin receptor (sOB-R) concentrations. Eighteen male participants completed two different exercise sessions with intensities of 25% and 65% maximal aerobic capacity (VO₂max). In addition to the energy expenditure during exercise sessions being measured, blood samples were collected before exercise, and immediately, at 24 h, and at 48 h post-exercise to analyse sOB-R, leptin and insulin levels. At 24 h post-exercise, sOB-R and leptin concentrations at the 65% VO₂max were significantly different from those at the 25% VO₂max. Leptin levels at 48 h post-exercise were also significantly lower for the 65% VO₂max than for the 25% VO₂max (P < 0.01). In the 65% VO₂max session, the energy expenditure during exercise was significantly associated with leptin concentrations at 24 h and 48 h and sOB-R concentrations at 24 h post-exercise. However, no correlations were found between sOB-R and leptin at the three post-exercise time points. In conclusion, an acute bout of exercise with 920 kcal of output resulted in an increase in sOB-R levels at 24 h post-exercise. However, the changes in sOB-R levels due to an acute bout of exercise might not contribute to the delayed decrease observed for leptin.     

Effect of repeat-sprint training in hypoxia on post-exercise interleukin-6 and F2-isoprostanes

This investigation examined the oxidative stress (F₂-Isoprostane; F₂-IsoP) and inflammatory (interleukin-6; IL-6) responses to repeat-sprint training in hypoxia (RSH). Ten trained male team sport athletes performed 3(sets)*9(repetitions)*5?s cycling sprints in simulated altitude (3000?m) and sea-level conditions. Mean and peak sprint power output (MPO and PPO) were recorded, and blood samples were collected pre-exercise, and again at 8 and 60?min post-exercise. Both MPO and PPO were significantly reduced in hypoxia (compared to sea-level) in the second (MPO: 855?±?89 vs. 739?±?95?W, p?=?.006; PPO: 1024?±?114 vs. 895?±?112?W, p?=?.010) and third (MPO: 819?±?105 vs. 686?±?83?W, p?=?.008; PPO: 985?±?125 vs. 834?±?99?W, p?=?.008) sets, respectively. IL-6 was significantly increased from pre- to 1?h post-exercise in both hypoxia (0.7?±?0.2 vs. 2.4?±?1.4?pg/mL, p?=?.004) and sea-level conditions (0.7?±?0.2 vs. 1.6?±?0.3?pg/mL, p?d?=?0.80) suggesting higher IL-6 levels of post-hypoxia. F₂-IsoP was significantly lower 1?h post-exercise in both the hypoxic (p?=?.005) and sea-level (p?=?.002) conditions, with no differences between trials. While hypoxia can impact on exercise intensity and may result in greater post-exercise inflammation, it appears to have little effect on oxidative stress. These results indicate that team sport organisations with ready access to hypoxic training facilities could confidently administer RSH without significantly increasing the post-exercise inflammatory or oxidative stress response.     

Forearm oxygenation and blood flow kinetics during a sustained contraction in multiple ability groups of rock climbers

Currently, the physiological mechanisms that allow elite level climbers to maintain intense isometric contractions for prolonged periods of time are unknown. Furthermore, it is unclear whether blood flow or muscle oxidative capacity best governs performance. This study aimed to determine the haemodynamic kinetics of 2 forearm flexor muscles in 3 ability groups of rock climbers. Thirty-eight male participants performed a sustained contraction at 40% of maximal voluntary contraction (MVC) until volitional fatigue. Oxygen saturation and blood flow was assessed using near infrared spectroscopy and Doppler ultrasound. Compared to control, intermediate, and advanced groups, the elite climbers had a significantly (P < 0.05) higher strength-to-weight ratio (MVC/N), de-oxygenated the flexor digitorum profundus significantly (P < 0.05) more (32, 34.3, and 42.8 vs. 63% O₂, respectively), and at a greater rate (0.32, 0.27, and 0.34 vs. 0.77 O₂%·s^?1, respectively). Furthermore, elite climbers de-oxygenated the flexor carpi radialis significantly (P < 0.05) more and at a greater rate than the intermediate group (36.5 vs. 14.6% O₂ and 0.43 vs. 0.1O₂%·s^?1, respectively). However, there were no significant differences in total forearm ? blood flow. An increased MVC/N is not associated with greater blood flow occlusion in elite climbers; therefore, oxidative capacity may be more important for governing performance.     

Understanding mental toughness in Australian soccer: Perceptions of players,parents, and coaches

Abstract

This study was designed to investigate the effect of ingesting a glucose plus fructose solution on the metabolic responses to soccer-specific exercise in the heat and the impact on subsequent exercise capacity. Eleven male soccer players performed a 90 min soccer-specific protocol on three occasions. Either 3 ml · kg^?1 body mass of a solution containing glucose (1 g · min^?1 glucose) (GLU), or glucose (0.66 g · min^?1) plus fructose (0.33 g · min^?1) (MIX) or placebo (PLA) was consumed every 15 minutes. Respiratory measures were undertaken at 15-min intervals, blood samples were drawn at rest, half-time and on completion of the protocol, and muscle glycogen concentration was assessed pre- and post-exercise. Following the soccer-specific protocol the Cunningham and Faulkner test was performed. No significant differences in post-exercise muscle glycogen concentration (PLA, 62.99 ± 8.39 mmol · kg wet weight^?1; GLU 68.62 ± 2.70; mmol · kg wet weight^?1 and MIX 76.63 ± 6.92 mmol · kg wet weight^?1) or exercise capacity (PLA, 73.62 ± 8.61 s; GLU, 77.11 ± 7.17 s; MIX, 83.04 ± 9.65 s) were observed between treatments (P > 0.05). However, total carbohydrate oxidation was significantly increased during MIX compared with PLA (P < 0.05). These results suggest that when ingested in moderate amounts, the type of carbohydrate does not influence metabolism during soccer-specific intermittent exercise or affect performance capacity after exercise in the heat.     

Chronic ingestion of a low dose of caffeine induces tolerance to the performance benefits of caffeine

This study examined effects of 4 weeks of caffeine supplementation on endurance performance. Eighteen low-habitual caffeine consumers (<75 mg · day^?1) were randomly assigned to ingest caffeine (1.5–3.0 mg · kg^?1day^?1; titrated) or placebo for 28 days. Groups were matched for age, body mass, V?O_2peak and W_max (P > 0.05). Before supplementation, all participants completed one V?O_2peak test, one practice trial and 2 experimental trials (acute 3 mg · kg^?1 caffeine [precaf] and placebo [testpla]). During the supplementation period a second V?O_2peak test was completed on day 21 before a final, acute 3 mg · kg^?1 caffeine trial (postcaf) on day 29. Trials consisted of 60 min cycle exercise at 60% V?O_2peak followed by a 30 min performance task. All participants produced more external work during the precaf trial than testpla, with increases in the caffeine (383.3 ± 75 kJ vs. 344.9 ± 80.3 kJ; Cohen’s d effect size [ES] = 0.49; P = 0.001) and placebo (354.5 ± 55.2 kJ vs. 333.1 ± 56.4 kJ; ES = 0.38; P = 0.004) supplementation group, respectively. This performance benefit was no longer apparent after 4 weeks of caffeine supplementation (precaf: 383.3 ± 75.0 kJ vs. postcaf: 358.0 ± 89.8 kJ; ES = 0.31; P = 0.025), but was retained in the placebo group (precaf: 354.5 ± 55.2 kJ vs. postcaf: 351.8 ± 49.4 kJ; ES = 0.05; P > 0.05). Circulating caffeine, hormonal concentrations and substrate oxidation did not differ between groups (all P > 0.05). Chronic ingestion of a low dose of caffeine develops tolerance in low-caffeine consumers. Therefore, individuals with low-habitual intakes should refrain from chronic caffeine supplementation to maximise performance benefits from acute caffeine ingestion.     

Effects of carbohydrate ingestion on skill maintenance in squash players

Abstract

The effects of carbohydrate (CHO) ingestion during sports which require high levels of motor and cognitive skill, such as squash, have produced conflicting results. This study aimed to explore the effect of CHO ingestion on squash skill following short duration exercise simulating the demands of squash play. Sixteen male squash players of a high standard were recruited. Following a VO₂max test, and familiarisation trial, subjects completed two further trials assessing skill pre- and post-exercise designed to simulate the demands of squash play. A squash skill test assessed accuracy of the forehand and backhand straight drives. Exercise consisted of 20 minutes of shuttle running at 82(±5)% HR_max, and 9 minutes of ghosting at 94(±4)% HR_max. Capillary blood samples (20 µl) were taken at five intervals for measurement of glucose and lactate. Cognitive function was measured with choice visual and auditory reaction time (RT) tests pre- and post-exercise, as was forearm wrist flexor MVC and fatigue profile. CHO drink (6.4% CHO) or matched placebo (PL) were administered after the initial skill test (500 ml), after the shuttle running (250 ml), and after the ghosting (250 ml) in a double blind crossover design. There was no overall effect of CHO ingestion on skill maintenance (p=0.10) however, significantly fewer balls landed outside the scoring zone (p=0.03) on the CHO ingestion trial. There was no change of visual RT pre- to post-exercise on PL (+0.01±0.03s), but a significant improvement (?0.07±0.05s) was observed in the CHO trial. Auditory RT improved pre- to post-exercise during both trials. MVC and fatigue profile of the wrist flexors was not different between trials but showed a force decrement pre- to post-exercise (p<0.05). A significant difference in blood glucose was observed between trials (p<0.01) but blood lactate response during both trials was similar. These results lend some support to a beneficial effect of CHO ingestion on skill during game sports.     

The effect of menstrual cycle on 2000-m rowing ergometry performance

Abstract

The aim of this study was to examine the effect of menstrual cycle phase on 2000-m rowing ergometry performance. Since high concentrations of oestrogen, indicative of the mid-luteal phase of the menstrual cycle, tend to decrease glycogen utilization and reduce blood lactate concentration, it was predicted that time taken to complete a 2000-m rowing trial would be shorter in the mid-luteal phase. Ten eumenorrhoeic, recreationally trained, female volunteers (mean age 33.0 years, s=7.1) completed 2000-m time trials on a Concept 2 rowing ergometer, in both the mid-follicular and mid-luteal phases of their menstrual cycle. In each phase, a 3-min incremental rowing protocol was used to determine a blood lactate concentration of 4 mmol · l^?1 (T _lac-4mM) and maximum oxygen consumption (VO_2max); a five-stroke maximal test was used to establish maximal power. Order of testing was randomized for menstrual cycle phase. Variables (T _lac-4mM, VO_2max, maximal power) were correlated with speed in the 2000-m time trials, and the effect of menstrual cycle phase on these variables was examined. A blood lactate concentration of 4 mmol · l^?1 occurred at a significantly higher mean exercise intensity (mid-luteal vs. mid-follicular: 169.1 W, s=39.1 vs. 159.0 W, s=38.3; P=0.033), heart rate (179 beats · min^?1, s=9 vs. 173 beats · min^?1, s=11; P=0.0047), and oxygen consumption (2.64 litres · min^?1, s=0.66 vs. 2.42 litres · min^?1, s=0.62; P=0.04) in the mid-luteal phase than in the mid-follicular phase. There was no significant difference (P=0.11) in 2000-m time trial speed according to menstrual cycle phase. In conclusion, although T _lac-4mM differed due to menstrual cycle phase, 2000-m rowing performance was unaffected. Further research into the effects of menstrual cycle on rowing performance of a longer duration, among a more homogenous group of females, is recommended.     

Tear osmolarity is sensitive to exercise-induced fluid loss but is not associated with common hydration measures in a field setting

This investigation (i) examined changes in tear osmolarity in response to fluid loss that occurs with exercise in a field setting, and (ii) compared tear osmolarity with common field and laboratory hydration measures. Sixty-three participants [age 27.8 ± 8.4 years, body mass 72.15 ± 10.61 kg] completed a self-paced 10 km run outside on a predetermined course. Body mass, tear fluid, venous blood and urine samples were collected immediately before and after exercise. Significant (p < 0.001) reductions in body mass (1.71 ± 0.44%) and increases in tear osmolarity (8 ± 15 mOsm.L^?1), plasma osmolality (7 ± 8 mOsm.kg^?1), and urine specific gravity (0.0014 ± 0.0042 g.mL^?1; p = 0.008) were observed following exercise. Pre- to post-exercise change in tear osmolarity was not significantly correlated (all p > 0.05) with plasma osmolality (r_s = 0.24), urine osmolality (r_s = 0.14), urine specific gravity (r_s = 0.13) or relative body mass loss (r = 0.20). Tear osmolarity is responsive to exercise-induced fluid loss but does not correlate with the changes observed using other common measures of hydration status in the field setting. Practitioners shouldn’t directly compare or replace other common hydration measures with tear osmolarity in the field.

Abbreviations: BML: Body Mass Loss; CV: Coefficient of Variation; P_osm: Plasma osmolality; SD: Standard Deviation; T_osm: Tear Osmolarity; U_osm: Urine Osmolality; USG: Urine Specific Gravity; WBGT: Wet bulb globe thermometer     

Precision of the optimized carbon monoxide rebreathing method to determine total haemoglobin mass and blood volume

Abstract

The aim of this study was to evaluate the influence of different apparatuses, procedures and calculations on the precision of the optimized carbon monoxide method. Total haemoglobin mass was determined twice on consecutive days in 11 subjects using both venous and capillary blood samples. To estimate loss of carbon monoxide due to exhalation, carbon monoxide concentration was measured by two portable carbon monoxide analysers (Fluke CO-220, Fluke, Norwich, UK and Pac 7000 Carbon Monoxide, Draeger Safety, Northumberland, UK) and alveolar ventilation was specified using an automated metabolic gas analysis system (Cosmed Quark b2, Cosmed, Rome, Italy). Blood volume was derived from total haemoglobin mass using haemoglobin concentration and haematocrit obtained in both the supine and the seated position. Two different formulae to calculate blood volume were also compared. Precision was good for both total haemoglobin mass and blood volume measurements performed on consecutive days (typical error < 2%). Using Fluke CO-220 analyser, an estimated alveolar ventilation and capillary blood, total haemoglobin mass (917±136 g) was similar when compared to the Pac 7000 Draeger CO-analyser (904±137 g; mean bias –13 g with 95% limits of agreement –26 to + 1 g, P=0.76), specified alveolar ventilation (911±132 g, mean bias –6 g with 95% limits of agreement –18 g to + 6 g, P =0.87) and venous blood (917±134 g, mean bias 0 g with 95% limits of agreement –38 to + 38 g, P=0.99), respectively. Blood volume determination was also not significantly affected by the supine vs. seated position (6.7±0.8 l vs. 6.6±0.8 l, P=0.56) but can deviate by ~0.6 l (P=0.01) depending on the formula applied. Thus, the good precision of the assessment of total haemoglobin mass and blood volume using the optimized carbon monoxide rebreathing method is not significantly influenced by the make of CO analyser, method of obtaining alveolar ventilation, blood sampling method and subject position, but should for longitudinal monitoring purposes use the same formula.     

Dietary nitrate supplementation does not improve cycling time-trial performance in the heat

This investigation examined the effect of beetroot juice (BR) supplementation, a source of dietary nitrate (NO₃^?), on cycling time-trial (TT) performance and thermoregulation in the heat. In a double-blind, repeated-measures design, 12 male cyclists (age 26.6 ± 4.4 years, VO_2peak 65.8 ± 5.5 mL.kg^?1.min^?1) completed four cycling TTs (14 kJ.kg^?1) in hot (35°C, 48% relative humidity) and euthermic (21°C, 52%) conditions, following 3 days supplementation with BR (6.5 mmol NO₃^? for 2 days and 13 mmol NO₃^? on the final day), or NO₃^–depleted placebo (PLA). Salivary NO₃^? and nitrite, core (T_c) and mean skin temperature (T_sk) were measured. Salivary NO₃^? and nitrite increased significantly post-BR supplementation (p < 0.001). Average TT completion time (mm:ss) in hot conditions was 56:50 ± 05:08 with BR, compared with 58:30 ± 04:48 with PLA (p = 0.178). In euthermic conditions, average completion time was 53:09 ± 04:35 with BR, compared with 54:01 ± 04:05 with PLA (p = 0.380). The TT performance decreased (p < 0.001), and T_c (p < 0.001) and T_sk (p < 0.001) were higher in hot compared with euthermic conditions. In summary, BR supplementation has no significant effect on cycling TT performance in the heat.     

Physiological responses and performance in a simulated trampoline gymnastics competition in elite male gymnasts

Abstract

Physiological responses and performance were examined during and after a simulated trampoline competition (STC). Fifteen elite trampoline gymnasts participated, of which eight completed two routines (EX1 and EX2) and a competition final (EX3). Trampoline-specific activities were quantified by video-analysis. Countermovement jump (CMJ) and 20 maximal trampoline jump (20-MTJ) performances were assessed. Heart rate (HR) and quadriceps muscle temperature (T_m) were recorded and venous blood was drawn. A total of 252 ± 16 jumps were performed during the STC. CMJ performance declined (P < 0.05) by 3.8, 5.2 and 4.2% after EX1, EX2 and EX3, respectively, and was 4.8% lower (P < 0.05) than baseline 24 h post-competition. 20-MTJ flight time was ~1% shorter (P < 0.05) for jump 1–10 after EX2 and 24 h post STC. T_m increased (P < 0.05) to ~39°C after the warm-up, but declined (P < 0.05) 1.0 and 0.6ºC before EX2 and EX3, respectively. Peak HR was 95–97% HR_max during EX1-3. Peak blood lactate, plasma K⁺ and NH₃ were 6.5 ± 0.5, 6.0 ± 0.2 mmol · l^?1 and 92 ± 10 µmol · l^?1, respectively. Plasma CK increased (P < 0.05) by ~50 and 65% 0 and 24 h after STC. In conclusion, a trampoline gymnastic competition includes a high number of repeated explosive and energy demanding jumps, which impairs jump performance during and 24 h post-competition.     

Effect of a carbohydrate mouthwash on running time-trial performance

Abstract

The aim of the present study was to determine the effect of a carbohydrate mouthwash on running time-trial performance. On two separate occasions, seven recreationally active males ([Vdot]O_2max 57.8 ml · kg^?1 · min^?1, s = 3.7) completed a preloaded (15 min at 65%[Vdot]O_2max) time-trial of 45 min in duration on a motorized treadmill. At 6-min intervals during the preload and time-trial, participants were given either a 6% maltodextrin, 3% lemon juice solution (carbohydrate trial) or a 3% lemon juice placebo mouthwash (placebo trial) in a double-blind, randomized crossover design. Heart rate, oxygen consumption ([Vdot]O₂), respiratory exchange ratio (RER), and ratings of perceived exertion (RPE) were measured during the preload, and blood glucose and lactate were measured before and after the preload and time-trial. There were no significant differences in distance covered between trials (carbohydrate: 9333 m, s = 988; placebo: 9309 m, s = 993). Furthermore, there were no significant between-trial differences in heart rate and running speed during the time-trial, or [Vdot]O₂, RER or RPE during the preload. Blood lactate and glucose increased as a result of the exercise protocol, with no between-trial differences. In conclusion, there was no positive effect of a carbohydrate mouthwash on running performance of ～1 h duration.     

Reliability of laser Doppler,near-infrared spectroscopy and Doppler ultrasound for peripheral blood flow measurements during and after exercise in the heat

This study examined the test-retest reliability of near-infrared spectroscopy (NIRS), laser Doppler flowmetry (LDF) and Doppler ultrasound to assess exercise-induced haemodynamics. Nine men completed two identical trials consisting of 25-min submaximal cycling at first ventilatory threshold followed by repeated 30-s bouts of high-intensity (90% of peak power) cycling in 32.8 ± 0.4°C and 32 ± 5% relative humidity (RH). NIRS (tissue oxygenation index [TOI] and total haemoglobin [tHb]) and LDF (perfusion units [PU]) signals were monitored continuously during exercise, and leg blood flow was assessed by Doppler ultrasound at baseline and after exercise. Cutaneous vascular conductance (CVC; PU/mean arterial pressure (MAP)) was expressed as the percentage change from baseline (%CVC_BL). Coefficients of variation (CVs) as indicators of absolute reliability were 18.7–28.4%, 20.2–33.1%, 42.5–59.8%, 7.8–12.4% and 22.2–30.3% for PU, CVC, %CVC_BL, TOI and tHb, respectively. CVs for these variables improved as exercise continued beyond 10 min. CVs for baseline and post-exercise leg blood flow were 17.8% and 10.5%, respectively. CVs for PU, tHb (r² = 0.062) and TOI (r² = 0.002) were not correlated (P > 0.05). Most variables demonstrated CVs lower than the expected changes (35%) induced by training or heat stress; however, minimum of 10 min exercise is recommended for more reliable measurements.     

A single session of treadmill running has no effect on plasma total ghrelin concentrations

Abstract

Ghrelin is a hormone that stimulates hunger. Intense exercise has been shown to temporarily suppress hunger after exercise. In the present study, we investigated whether post-exercise hunger suppression is mediated by reduced plasma total ghrelin concentrations. Nine men and nine women participated in the study. Their mean physical characteristics were as follows: age 24.8 (s _x  = 0.9) years, body mass index 22.9 (s _x  = 0.6) kg · m^?2, maximal oxygen uptake ([Vdot]O_2max) 57.7 (s _x  = 2.2) ml · kg^?1 · min^?1. The participants completed two 3-h trials (exercise and control) on separate days in a randomized balanced design after overnight fasts. The exercise trial involved a 1-h treadmill run at 73.5% of [Vdot]O_2max followed by 2 h of rest. The control trial consisted of 3 h of rest. Blood samples were collected at 0, 0.5, 1, 1.5, 2, and 3 h. Total ghrelin concentrations were determined from plasma. Hunger was assessed following blood sampling using a 15-point scale. The data were analysed using repeated-measures analysis of variance. Hunger scores were lower in the exercise trial than in the control trial (trial, P = 0.009; time, P < 0.001; trial × time, P < 0.001). Plasma total ghrelin concentrations did not differ between trials. These findings indicate that treadmill running suppresses hunger but this effect is not mediated by changes in plasma total ghrelin concentration.     

The sensitivity of the alternative maximal accumulated oxygen deficit method to discriminate training status

The purpose of the study was to investigate the sensitivity of an alternative maximal accumulated oxygen deficit (MAOD_ALT) method to discriminate the “anaerobic” capacity while comparing: least trained (LT) participants (n = 12), moderately trained (MT) participants (n = 12), endurance trained (ET) participants (n = 16), and rugby (RG) players (n = 11). Participants underwent a graded exercise test on a treadmill and a supramaximal effort for assessing MAOD_ALT. MAOD_ALT was calculated as the sum of oxygen equivalents from the phosphagen and glycolytic metabolic pathways. MAOD_ALT was significantly higher (P < 0.05) in RG (64.4 ± 12.1 mL · kg^?1) than in ET (56.8 ± 5.4 mL · kg^?1; effect size [ES] = 0.77; +13.5%), MT (53.8 ± 5.3 mL · kg^?1; ES = 1.08; +19.8%), and LT (49.9 ± 4.5 mL · kg^?1; ES = 1.50; +36.4%). In addition, the magnitude-based inference analysis revealed that MAOD_ALT was likely (LT vs. MT), very likely (MT vs. RG, and ET vs. RG) and most likely (LT vs. ET, and LT vs. RG) different between all groups, except for MT and ET, which presented an unclear difference. In conclusion, MAOD_ALT was sensitive enough to distinguish the “anaerobic” capacity in individuals with different training status, especially for RG players compared with LT participants and MT participants.