大鼠阴道上皮细胞对白色念珠菌生长抑制作用的初探

目的研究阴道上皮细胞对白色念珠菌生长的影响。方法以雌性SD大鼠动情间期阴道上皮细胞作为效应细胞,白色念珠菌芽生孢子作为靶细胞,将效靶细胞共同孵育8h,观察念珠菌生长密度,检测念珠菌摄取3H-葡萄糖的能力,电镜观察其超微结构的改变。结果阴道上皮细胞与白色念珠菌细胞共同孵育后,念珠菌生长密度降低;效靶细胞比例为40:1,20:1,10:1,1:1时,念珠菌摄糖抑制率分别为52.34%,32.56%,28.73%,18.46%;电镜观察,单独培养的白色念珠菌结构完整,细胞壁、细胞膜及细胞器结构清晰,而与阴道细胞共孵育后的白色念珠菌细胞壁溶解破坏,细胞膜连续性破坏,细胞器肿胀溶解。结论大鼠阴道上皮细胞对白色念珠菌具有天然的生长抑制作用。     

西藏地区乙型肝炎病毒基因及其亚型的研究

目的 探讨西藏地区乙型肝炎病毒及其亚型;方法 随机选择HBsAg阳性携带者34例,慢性乙型肝炎患者9例,应用FQ-PCR方法检测血清HBV-DNA含量;采用聚合酶链反应限制性酶长度多态性分析法,通过PCR扩增出目标基因片段,然后用特定的限制性内切酶进行酶切,根据酶切图谱进行基因型及亚型的测定.结果 43例HBV-DNA定量阳性血中c基因型占83.72%(36/43例);B型占6.9%(3/43);B、C混合型占4.65%(2/43);D型占4.65%(2/43).B基因型的亚型中Ba6.91%(5/43);B、C型混合型中C2亚型4.65%(2/43).结论 西藏地区HBV基因型以C型为主,B型次之,B型亚型以Ba占优势.     

三种贝母核型的比较研究

The present paper deals with a comparative karyotypic study of three species in Fri- tillaria-F. thuncergii Miq., F. anhuiensis S.  . Chen et S. F. Yin and F. hupehensis Hsiao et K. C. Hsia.  The karyotype of F. anhuiensis S. C. Chen et S. F. Yin is first reported.       The karyotypes of the three species of Fritillaria are rather similar, all with K(2n)=24= 2m+2sm+12t+4st+4m (SAT), showing a close interspecific relationship.  They all have two pairs of st chromosomes, one of which is the third chromosome in all the three species studied, but the other is the seventh in F. thunbergii Miq, the eighth in F. anhuiensis S. C. Chen et S. F. Yin, and the fifth in F. hupehensis Hsiao et K. C. Hsia.  It tells us that there are some differences in their karyotypes.  All of the three species possess two pairs of satellite chromosomes with the satellites located on the long arms. A heterochromatic zone is found sometimes on long arms of No. IX chromosome in each species of Fritillaria and on one of No. I chromosomes in both F. thun- bergii Miq. and F. anhuiensis S. C. Chen et S. F. Yin, a chromosome polymorphism occurring between populations of Fritillaria. In addition, three B chromosomes are always found in most root-tip cells of F. hupehensis Hsiao et K. C. Hsiao.     

Evaluation of Low Blood Lead Levels and Its Association with Oxidative Stress in Pregnant Anemic Women: A Comparative Prospective Study

To correlate blood lead levels (BLLs) and oxidative stress parameters in pregnant anemic women. A total of 175 pregnant women were found suitable and included for this study. Following WHO criteria, 50 each were identified as non-anemic, mild anemic and moderate anemic and 25 were severe anemic. The age of all study subjects ranged from 24–41 years. At admission, BLLs and oxidative stress parameters were estimated as per standard protocols and subjected with ANOVA, Pearson correlation analysis and cluster analysis. Results showed significantly (p < 0.01) high BLLs, zinc protoporphyrin (ZPP), oxidized glutathione (GSSG), lipid peroxide (LPO) levels while low delta aminolevulinic acid dehydratase (δ-ALAD), iron (Fe), selenium (Se), zinc (Zn), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), red blood cell (RBC) count, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (TAC) in all groups of anemic pregnant women as compared with non anemic pregnant women. In all groups of pregnant women, BLLs showed significant (p < 0.01) and direct association with ZPP, GSSG and LPO while inverse relation with δ-ALAD, Fe, Se, Zn, Hb, Hct, MCV, MCH, MCHC, RBC, GSH, SOD, CAT and TAC. Study concluded that low BLLs perturb oxidant-antioxidant balance and negatively affected hematological parameters which may eventually Pb to Fe deficiency anemia during pregnancy.     

Study on Impact of Iron and Folic Acid on the Plasma Trace Minerals in Pregnant Anemic Women

Iron deficiency anemia is one of the causes that lead to significant mortality and morbidity among pregnant women and fetus. The present study was undertaken to explore oral iron supplementation can modify the metal contents in pregnant anemic women. Iron and folic acid supplementations was given to 500 anemic women (mild = 200, moderate = 200, and severe = 100) and 100 age matched non-anemic controls daily for 100 days. Blood index values and plasma trace minerals were estimated as per standard protocols. Haemoglobin and ferritin levels were found significantly increased (p < 0.001) in anemic and control subjects after treatment. Moreover, the serum transferring receptor levels and total iron binding capacity were found significantly decreased in all treated groups. Iron (Fe), zinc (Zn) and copper (Cu) levels were found increased (p < 0.01) after oral iron supplementation groups. Moreover, selenium (Se) manganese (Mn) and were found to be decreased in all treated groups. Data provides the conclusion that iron and folic acid supplementation recovered the essential trace minerals, except manganese, which may lead to various complications including peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus.     

Evaluation of Oxidative Stress and Antioxidant Status in Pregnant Anemic Women

The present study was conducted to investigate the oxidant–antioxidant status in iron deficient pregnant anemic women. One hundred thirty pregnant women with iron deficiency anemia (IDA) were divided into three groups, namely mild (50), moderate (50) and severe (30) anemic along with pregnant healthy women as controls (50). The complete blood count, plasma lipid peroxidation products, enzymatic and non-enzymatic antioxidants were measured according to respective protocols. The levels of complete blood count, iron, ferritin along with antioxidant enzymes namely catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and reduced glutathione were significantly reduced in all IDA groups. However, the level of oxidized glutathione, lipid peroxides, protein carbonyls, conjugated dienes were found significantly increased in all anemic patients. Antioxidant vitamins, namely C, E and A were also found significantly decreased in IDA patients. On the basis of our results, it may be concluded that IDA tends to increase the pro-oxidant components, which may result in various complications including peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus.     

芸薹属多倍体植物基因组进化的RAPD分析

多倍化是促进高等植物发生进化的重要力量。为了更清楚地了解多倍体在形成之后其基因组是如何进化的，利用38个随机引物对芸薹属Brassica L.禹氏三角（U’Triangle）中的多倍体物种及其祖先二倍体物种进行了研究。根据扩增出的273条带计算了遗传距离，并用UPGMA法进行了聚类分析。结果发现，二倍体物种B.campestris（AA）与B.oleracea（CC）的亲缘关系比与B.nigra（BB）的要近;异源多倍体B.napus（AACC）比起其二倍体祖先之一B.campestris（AA）与另一个     

Bacillus Megaterium在Vc二步发酵中伴生活性初步研究

在维生素C二步发酵中,Bacillusmegaterium作为伴生菌能有效促进Gluconobacteroxydans的生长和产VitaminC的前体 2 酮基 L 古龙酸(简称 2 KLG)。实验室利用低能离子对出发菌BP5 2进行诱变选育,获得一株B.megaterium突变体GB82,此突变体与G.oxydans组成的混合体系GAB,比原始混合体系 2 980产酸水平高 1 0个百分点以上。比较研究了GB82与原始B.megateriumBP5 2分泌的生物活性物质对产酸菌G.oxydans产 2 KLG的影响,据此提出此活性物质可能是某种蛋白,且此蛋白在极端条件下会部分失活的观点;并且通过RandomAmplifiedPolymorphicDNA(RAPD)分析揭示了低能离子注入造成的二者基因水平上的差异。     

中西文化对科学规范影响的比较研究

通过问卷调查发现,中西方科技人员在对待默顿规范的态度上存在着较大差异。究其原因与中西方的文化传统有较大的相关性;文化既然影响了科技人员的价值观,就会进一步影响其行为规范。其实,默顿规范产生于特定的历史和文化背景,这进一步说明文化对科学规范的影响;在不同的文化背景和不同的情境下科技人员应遵循不同的科学规范。这些研究为新时期我国科学规范的制定奠定了一定的学理基础。     

无藻萍的RAPD分析及其在三膘组满江红种间关系研究中的应用

从满江红Azolla Lam.萍-藻共生体中提取DNA进行的RAPD系统分析通常忽视了满江红样品的异质性。本研究通过获得无藻的满江红，比较有藻萍、无藻萍和离体藻之间的RAPD指纹图谱。发现从有藻萍中提取DNA的扩增反应来源于萍藻双方DNA的共同影响。依引物和植物样本的不同，共生双方对扩增产物的贡献结果不同，说明了用无藻萍进行RAPD检测的重要性。对满江红三膘组5个种的11个无藻萍样本进行了RAPD分析，由9个引物产生的127个DNA多态片段用于计算样本间的Jaccard相似系数和UPGMA树状聚类图。结果     

芒苞草的细胞学研究

芒苞草的染色体数目为2n=38，染色体很小。染色体数目表明芒苞草与百合科蜘蛛抱蛋属Aspidistra。和开口箭属Tupistra有一定联系，这也得到形态特征的支持。