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1.
Anjali C. Warrier Narasimha Y. Rao Devdutta S. Kulpati Tarun K. Mishra Bhaskar C. Kabi 《Indian journal of clinical biochemistry : IJCB》1995,10(1):9-13
There was increase in erythrocyte lipid peroxidation and adenosine deaminase (ADA) activity in red blood cells (RBC's), white
blood cells (WBC's) and plasma, in 30 patients of confirmed diabetes mellitus, 10 each of insulin dependent diabetes mellitus
(IDDM or type I), non-insulin dependent diabetes mellitus (NIDDM or type II) and diabetes with ketoacidosis when compared
with 20 healthy individuals (controls). Glycosylated hemoglobin A1c%, plasma free fatty acid (FFA) and glycerol levels were
also elevated in all the cases when compared with controls. The increase was more in diabetic ketoacidosis group. Significant
positive correlations were seen between erythrocyte lipid peroxidation and ADA activities in RBC's, WBC's and plasma. It is
suggested that decreased tissue adenosine levels due to increase in ADA activity, is related to the severity of hyperglycemia
and lipid peroxidation in diabetes mellitus. 相似文献
2.
N. P. Suryawanshi A. K. Bhutey A. N. Nagdeote A. A. Jadhav G. S. Manoorkar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):126-130
The study was designed to find out the correlation between lipid peroxidation, lipoprotein levels to severity and complication
of diabetes mellitus. Degree of lipid peroxidation was measured in terms of malondialdehyde (MDA) along with lipid profile
and blood glucose in diabetes mellitus. It is categorised into insulin dependent diabetes mellitus (IDDM), non insulin dependent
diabetes mellitus (NIDDM) and diabetes mellitus(DM) with complication.
Total 112 known diabetic patients and 52 non-diabetic controls were studied. These patients were grouped as per the concentration
of fasting blood glucose level i.e. controlled, poorly controlled, and uncontrolled group. There are significant increase
in the lipid peroxide (MDA) and lipid profile except HDL cholesterol which is decreased, has been found in all groups as compared
to controls In NIDDM group lipid peroxidation was markedly increased than IDDM group and it was higher in DM with complications.
Other finding observed was that the level of lipid peroxide increased as per the increase in concentration of blood glucose.
The increase lipid peroxidation in the hyperglycemic condition may be explained, as the superoxide dismutase enzyme which
is antioxidant becomes inactive due the formation of superoxide radical within the cell. Maximum lipid peroxidation leads
to the damage of the tissue and organs which results into complication in diabetic patients. High levels of total cholesterol
appear due to increased cholesterol synthesis. The triglyceride levels changes according to the glycemic, control. The increase
may be due to overproduction of VLDL-TG.
It is concluded that good metabolic control of hyperglycemia will prevent in alteration in peroxidation and the lipid metabolism,
which may help in good prognosis and preventing manifestation of vascular and secondary complication in diabetes mellitus. 相似文献
3.
Sanjukta Mishra Jyotirmayee Bahinipati RajLaxmi Sarangi Soumya Ranjan Mohapatra Swarnalata Das Amaresh Mishra 《Indian journal of clinical biochemistry : IJCB》2023,38(2):151
MicroRNAs (miRNAs) are small endogenous, non-coding RNA molecules that can modulate the expression of their target genes. Since its discovery, an enormous breakthrough has been established regarding its biogenesis and pathophysiological action, which has revolutionized the field of molecular biology. In addition, recent studies have identified the existence of stable extracellular/circulating miRNAs tissues and in biological fluids like blood where they are safeguarded from endogenous ribonuclease activity. Type 2 diabetes mellitus (T2DM) has emerged as a prime health issue worldwide. Incidence has increased considerably over the past decade. There are various tests that have been employed to diagnose T2DM. But for early detection and development, the establishment of biomarkers are of paramount importance. Contemporary evidence also validates the signature of a set of this epigenetic factor miRNA in the development of various diseases, including T2DM. This article reviews the contemporary corroboration associating miRNAs and T2DM and emphasizes the potential role of miRNA as a circulatory biomarker that could alert the growing prevalence of T2DM. Also, it acknowledges the valuable compendium of information regarding biogenesis and functional role of circulating miRNA in insulin resistance which is intimately linked to T2DM.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12291-022-01069-1. 相似文献
4.
M. Maneesh H. Jayalakshmi T A Singh Amit Chakrabarti 《Indian journal of clinical biochemistry : IJCB》2006,21(1):165-168
In view of association of diabetes mellitus and male infertility, present study was designed to investigate the functional
status of hypothalamic pituitary gonadal (HPG) axis in diabetic men. Thirty-five diabetic men (BMI 22.24±0.21) in the age
group 20–40 (30.6±4.7) years were selected. Twenty-five healthy men (BMI 23.85±0.25), in the same age group (29.5±4.8) served
as control. Blood samples were collected for hormonal and biochemical estimations. Diabetic men had significantly low serum
testosterone with low LH and FSH, hypercholesterolemia, hypertriglyceridemia, hypoalbuminemia and increased oxidative stress.
Low serum testosterone in diabetic men was accompanied by low LH and FSH; the inability of the pituitary gland to respond
appropriately to a decline in testosterone implying central effect of high serum glucose on the interaction between the nervous
and endocrine system. Nutritional deficiency, increased oxidative stress and increased aromatase activity due to excessive
body fat might have also contributed to low serum testosterone. 相似文献
5.
A. Madhavi Reddy Y. N. Rao Yogendra Singh Alpana Saxena 《Indian journal of clinical biochemistry : IJCB》1995,10(2):66-71
Adenosine deaminase and protein tyrosine phosphatase activities in liver and peritoneal macrophages were estimated in control,
streptozotocin induced diabetes and insulin treated diabetic groups of Swiss albino mice. Both the enzyme levels were elevated
while glycogen content decreased in the liver of diabetic group of animals as compared to controls, while in insulin treated
group, they were comparable to control levels. In macrophages also, both adenosine deaminase which plays an important role
in their maturation and protein tyrosine phosphatase enzyme involved in their activation were high in diabetic group, whereas
in insulin treated diabetic group, the values were comparable to control levels. *** DIRECT SUPPORT *** A00FN011 00003 相似文献
6.
The present work identifies a high incidence of oral cancer in south Karnataka. The primary reason for this could be due to
extensive addiction to tobacco alone or tobacco and alcohol in most of these patients. The next in intensity was cancer of
the breast and cervix among the cancer patients surveyed in south Karnataka. Majority of these patients had no specific addiction
and the exact reason for this high incidence remains unclear. Only a small percentage of patients exhibited other types of
organ related cancer. It has been reported that the incidence percentage of diabetes mellitus and cancer in India, parallel
each other. However, we observed a low incidence of diabetes mellitus patients also having cancer in south Karnataka. Though
the exact reason for this remains unclear an attempt has been made here to explain this phenomenon, hypothetically using information
in literature, which suggests a suppression of cellular regenerative activity by sugar and sugar phosphates. Cellular regenerative
activity is well known to be a prerequisite for cancer. 相似文献
7.
Lipid peroxidation by measurement of Thiobarbituric Acid Reactive Substances (TBARS) and antioxidant status by determining
the activities of the enzymes, Catalase (Cat), Glutathione Peroxidase (GPx) and Superoxide Dismutase (SOD) and the level of
Reduced Glutathione (GSH) in the erythrocytes of patients with type 2 Diabetes Mellitus were investigated. It was observed
that the level of TBARS in the erythrocytes was increased by 50% showing a significant generation of free radicals in the
erythrocytes of these patients. The activities of both Cat and SOD were enhanced while that of GPx was not altered. The level
of GSH was also not changed. 相似文献
8.
Savita Singh Tenzin Kyizom K. P. Singh O. P. Tandon S. V. Madhu 《Indian journal of clinical biochemistry : IJCB》2008,23(4):365-368
A distinguishable feature of type 2 diabetes besides hyperglycemia and deranged lipid profile is an impaired insulin secretion,
peripheral insulin resistance and obesity which has become a major health concern worldwide. India with an estimated 31million
diabetics in 2000 and 79mllions by the yr 2030 has the highest number of type 2 diabetics in the world. In this study, we
aimed to see if yoga-asanas and pranayamas have any influence in modifying certain biochemical parameters. Sixty patients
of uncomplicated type 2 diabetes (age 35–60 yrs of 1–10 yrs duration) were divided into two groups: Group 1 (n=30): performed
yoga along with the conventional hypoglycemic medicines and group 2 (n=30): patients who only received conventional medicines.
Duration of the study was 45 days. Basal recordings of blood glucose (fasting and post-prandial), lipid profile and serum
insulin were taken at the time of recruitment and the second reading after forty five days. Results showed a significant improvement
in all the biochemical parameters in group 1 while group 2 showed significant improvement in only few parameters, thus suggesting
a beneficial effect of yoga regimen on these parameters in diabetic patients. 相似文献
9.
H. Surekha Rani G. Madhavi V. Ramachandra Rao B. K. Sahay A. Jyothy 《Indian journal of clinical biochemistry : IJCB》2005,20(2):75-80
Cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. An attempt has been made
to evaluate the risk factors for coronary heart disease in type II diabetics. In the present study the levels of fasting and
postprandial plasma glucose, total cholesterol, low density lipoproteins, triglycerides were high and the levels of high density
lipoproteins were low in the type II diabetics compared to controls. The markers of free radical induced injury i.e. malondialdehyde
and nitrite/nitrate were high while total antioxidant status a marker for antioxidant protection against reactive oxygen species
was low in diabetics compared to controls. The study therefore suggests the importance of assessing these markers of oxidative
stress and antioxidant capacity along with the other routine investigations in diabetic patients for initiating antioxidant
therapy in addition to primary and secondary preventive measures to mitigate the devastating consequences of diabetes leading
to coronary heart disease. 相似文献
10.
Y. K. Murali Ramesh Chandra P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》2004,19(2):202-204
Water extract of dry fruits ofTerminalia chebula (Hindi-Harda, Telugu-Karakkaya) at a dose of 200 mg/kg body weight improved the glucose tolerance as indicated by 44% of
reduction in the peak blood glucose at 2nd hour in glucose tolerance test in diabetic (streptozotocin induced) rats. Treatment of diabetic rats with an initial fasting
blood glucose of 253±9.4 mg/dl daily once with the water extract (200 mg/kg) for two weeks brought down the fasting blood
glucose to 123±8.4 mg/dl which is only slightly above the normal value. These results indicate that water extract of Terminalia
chebula improves glucose tolerance and brings down fasting blood glucose in diabetic rats. 相似文献
11.
Gauri S. Vahalkar Vijaya A. Haldankar 《Indian journal of clinical biochemistry : IJCB》2008,23(3):223-226
Glyco-oxidation is considered as a source of permanent, cumulative, oxidative damage to long lived proteins in ageing and
in diabetes. Although RBC depends solely on glucose for energy purpose, hyperglycemic state glycosylates hemoglobin, creates
oxidative stress and puts the cellular components at risk. In view of this, RBC membrane composition was analyzed in diabetic
patients. The results were compared with healthy age and sex matched control groups. When RBC membrane components such as
protein, sialic acid, phospholipids and cholesterol were determined in insulin dependent diabetes mellitus, a significant
rise in phospholipids and cholesterol and significant fall in sialic acid and protein content was noted. RBC membrane composition
showed pronounced alterations in insulin dependent diabetes mellitus. These changes were accompanied by higher levels of lipid
peroxidation products like Malondialdehyde. 相似文献
12.
Ivana Lapi Dunja Rogi Nora Nikolac Gabaj Katarina Kaji Nena Peran Lada Surjan Anamarija uras Valentina Cesar Kocijan Nada Bilopavlovi Fran Smai Mario tefanovi Ivanka Ostroki Leida Tandara Milena Krnjai-Tadijanovi Ivan Gornik Hrvoje Pintari Daniela Marasovi Krstulovi Blaenka Miki Dario Raheli 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2022,32(1)
IntroductionBased on the hypothesis that there is a substantial rate of adults with prediabetes and undiagnosed diabetes mellitus (DM), our aim was to perform haemoglobin A1c (HbA1c)-based screening in a cohort of Croatian adults and estimate the prevalence of prediabetes and undiagnosed DM according to American Diabetes Association criteria.Materials and methodsThis multi-center, cross-sectional study performed in six Croatian hospitals included 5527 patients aged 40 to 70 years admitted to the Emergency Department or undergoing a primary care check-up. Haemoglobin A1c was measured from leftover whole blood samples using the enzymatic method on either Alinity c or Architect c-series analyser (Abbott Laboratories, Chicago, USA). Haemoglobin A1c between 39-47 mmol/mol was classified as prediabetes, while ≥ 48 mmol/mol as undiagnosed DM.ResultsAfter exclusion of 435 patients with known DM, the final cohort included 5092 patients (median age 57; 56% males). A total of 882 (17.3%) patients had HbA1c values between 39 and 47 mmol/mol. There were 214 (4.2%) patients with HbA1c ≥ 48 mmol/mol. Prediabetes prevalence ranged from 14.2% to 20.5%, while undiagnosed DM from 3.3% to 7.3%, with statistically significant differences among settings (P < 0.001). Age-stratified analysis showed that prediabetes and undiagnosed DM prevalence increase with age (P < 0.001), being 25.4% and 5.8%, respectively, in patients aged 60 to 70 years.ConclusionUnderlying impairment of glucose metabolism was identified in about one in five adults, with significant number of patients with already overt DM. These results should serve as a starting point for further steps directed towards promotion of preventive measures for DM in Croatia. 相似文献
13.
Shivananda Nayak Arun Maiya Manjunath Hande 《Indian journal of clinical biochemistry : IJCB》2005,20(1):47-51
The role of treadmill exercise on blood glucose homeostasis in noninsulin dependent diabetes mellitus (NIDDM) were studied
using males between age of 45 and 60 years (X-52), who were clinically and biochemically-confirmed cases of NIDDM were taken
into study group. Control group comprised of 10 males between age group of 45 to 60 (X-53) years. All the subjects were assessed
by physician and were investigated to confirm diabetic status. The whole study period was extended for 6 weeks. The significant
decrease in postprandial blood sugar (44.4 mg% for the study group and 32.2mg% for the control group) with a significant inter
group difference (P<0.05) was observed. The mean decrease in fasting blood sugar (39.4mg% for the study group and 27.4mg%
for the control group), with a marginal inter group difference (P<0.05) was observed. The treadmill exercise was found to
be a definite tool in addition to drug and diet in glycemic control. 相似文献
14.
Ergen HA Zeybek U Gök O Karaali ZE 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2012,22(1):114-120
Introduction:
Non insulin dependent diabetes mellitus is the most common type of diabetes. Genetic factors, lipid profiles, hypertension are potential risk factors for diabetes mellitus. Adenosine binding cassette transporter proteins 1 (ABCA1) plays a role in cholesterol metabolism, especially high density lipoprotein (HDL-cholesterol). There are multiple mechanisms by which HDL-cholesterol can be atheroprotective, it is clear that the relative activity of ABCA1 plays a major role. We aimed to investigate association of ABCA1 C69T gene polymorphism with lipid levels in Turkish type 2 diabetic patients.Materials and methods:
After isolation of DNA by ethanol precipitation we determined ABCA1 gene polymorphism by using polimerase chain reaction - restriction fragment lenght polymorphism (PCR-RFLP) method in 107 type 2 diabetic patients and 50 healthy controls.Results:
We have observed that the frequency of TT genotype is significantly higher in healthy controls compared to patients (14% vs. 3%; P = 0.008). Also frequency of T allele was higher in controls than in patients (34% vs. 21%; P = 0.020; OR (95% CI) = 0.52 (0.30–0.88)). There was no association of lipid levels and ABCA1 C69T polymorphism subgroups.Conclusion:
We have found significantly higher frequency of both T allele and genotype in control group when compared to patients that made us think that T allele may be a protective factor against diabetes mellitus. But, we could not find a relationship between genotypes and lipid concentrations in our two groups. Larger studies will help us to understand the relationship between ABCA1 C69T genotype and lipid parameters in diabetes mellitus. 相似文献15.
Michael B. Adinortey Ben A. Gyan Jonathan P. Adjimani Philomena E. Nyarko Charity Sarpong Francis Y. Tsikata Alexander K. Nyarko 《Indian journal of clinical biochemistry : IJCB》2011,26(4):366-372
There is scanty information on the role of genetic factors, especially those relating to haptoglobin (Hp) phenotypes in the
expression of complications among diabetes mellitus patients in Ghana. In this study, we investigated whether there is any
association between Hp phenotypes and diabetic complications and to determine if association of the Hp phenotypes with diabetic
complications in Ghanaian diabetics differ from those in Caucasians. A total of 398 participants were randomly recruited into
the study. These comprised diabetic patients numbering 290 attending a diabetes Clinic in Ghana and 108 non-diabetic controls
from the same community. Analyses of the results indicate that most of the diabetics with complications were of the Hp 2–2
(35%) and Hp 2–1 (23.9%) phenotypes. Fewer diabetics were found to be of the Hp 2–1 M phenotype. The controls were mostly
of Hp 1–1 and Hp 2–1 M phenotypes. The odds ratio of having complications in a diabetic with an Hp 2–2 phenotype was 18.27
times greater than that for Hp 0–0. Hp 2–2 phenotype with its poor antioxidant activity may therefore be a useful predictor
for the propensity of an individual to develop diabetes complications. 相似文献
16.
Activities of human hepatic drug metabolizing enzymes N-acetyl transferase (NATS) had earlier been recognized as a cause of inter-individual variation in the metabolism of drugs.
Therefore acetylation of many drugs in human exhibit genetic polymorphism. The aim of the study was to investigate if acetylator
status predispose diabetic mellitus patients more to the complications of renal disease, One hundred and twenty (120) diabetics
consisting of (50) Type 1 (T1) and 70 Type 2 (T2) diabetes mellitus patients and 100 healthy individuals as controls were classified as slow or rapid acetylator using sulphamethazine
(SMZ) as an in vivo probe. The percentage acetylation, recovery of SMZ, creatinine clearance and presence of urinary albumin
were determined. A significant difference (P < 0.05) was observed in the percentage of SMZ acetylated between slow and rapid acetylators in control, T1 and T2 subjects. The ratios of slow to rapid acetylators for T1, T2 and control subjects were 1:4, 3:2 and 2:3 respectively. No significant differences were observed in the percentage of SMZ
recovered in the urine of slow and rapid acetylators that are diabetics. The difference in creatinine clearance of slow and
rapid acetylators in T1 and T2 were significant (P < 0.05). 29% out of 120 (24.2%) diabetics (T1 and T2) exhibited albuminuria out of which 25 (86.2%) had slow acetylator status. These findings suggest that slow acetylator status
in diabetes mellitus could be a predisposing factor in the development of renal complications. This underscores the need for
a rapid pharmacogenetic testing and therapeutic drug monitoring in such patients. However this inference could be further
validated with a larger sample size. 相似文献
17.
18.
Haseeb Ahmad Khan Samia Hasan Sobki Abdullah Saleh Alhomida Shaukat Ali Khan 《Indian journal of clinical biochemistry : IJCB》2007,22(1):65-70
This study reports the utilization of serum fructosamine and blood glucose for the screening of gestational diabetes mellitus
(GDM). Blood samples from 165 pregnant women were analyzed for fasting blood glucose (FBG), random blood glucose (RBG) and
serum fructosamine. The actual fructosamine levels were corrected for serum protein (c-Fruct) for more precise presentation.
Two cut-off values of FBG (>5.3 mmol/L and >7.0 mmol/L) and RBG (>7.8 mmol/L and >11.0 mmol/L) were used to classify hyperglycemic
subjects for subsequent evaluation. The average values±standard deviations for FBG, RBG and cFruct were 5.865±1.95, 7.767±3.21
and 2.387±0.47 mmol/L, respectively. FBG levels were significantly correlated with RBG (Pearson correlation=0.597, P<0.001).
Significant correlations were also observed between cFruct and FBG (Pearson correlation=0.673, P<0.001) or RBG (Pearson correlation=0.641,
P<0.001). Out of 165 subjects, 24 (14.5%) cases were classified as hyperglycemic on the basis of FBG>7.0 mmol/L or RBG>11.0
mmol/L; use of lower cut-off values resulted higher frequencies of hyperglycemia. Whereas, a combined criteria of FBG>5.3
mmol/L and cFruct >2.5 mmol/L predicted 35 patients as the most probable hyperglycemic as compared to 32 patients identified
using the criteria of RBG >7.8 mmol/L and cFruct >2.5 mmol/L. These criteria were associated with 4.8% and 3.6% false-positivity
at the expense of 3.6% and 3.0% false-negative outcomes, respectively. The levels of FBG, RBG and cFruct were significantly
higher in hyperglycemic groups (irrespective of grouping criteria) as compared to the respective normal groups. In conclusion,
these findings clearly indicate that the paired values of cFruct with FBG or RBG could help in filtering high-risk individuals
for OGTT and therefore avoiding a unnecessary OGTT. 相似文献
19.
Rajes Qvist Ikram Shah Ismail Karuthan Chinna Sekaran Muniandy 《Indian journal of clinical biochemistry : IJCB》2008,23(3):246-249
Although HbA1C is widely accepted as a useful index of mean blood glucose in type 2 diabetic patients its usefulness as screening test for
diabetes has been controversial. The present study was undertaken to determine whether the level of HbA1C predicted diabetes in a prediabetic group of subjects. Plasma lipids, oral glucose tolerance, HbA1C was determined in 90 normal control subjects, 57 offspring of one type 2 diabetes mellitus parent and 11 diagnosed type 2
diabetes mellitus individuals. The mean age of participants was 44.5 yrs (not significantly different amongst the three groups)
and the mean body mass index was 26.8 (not significantly different amongst the three groups). Two hours after a 75 g glucose
challenge, the offspring had a significantly higher plasma glucose level (mean = 7.1 mmol/L, p value = 0.002) than the normals. Similarly the HbA1C values were higher in the offspring than in the normals (mean = 5.78%, p value = 0.016). Besides the significantly higher values for oral glucose tolerance test and HbA1C, the diabetics also were significantly higher for triglycerides (mean = 2.25mmol/L), total cholesterol (mean = 6.24mmol/L)
and systolic blood pressure (mean = 138.45mm Hg) than the offspring (P value = 0.031, 0.006, 0.010) and the normals (P value = 0.026, 0.018, 0.002) respectively. The mean values of diastolic blood pressure, LDL cholesterol and HDL cholesterol
were not significantly different amongst the three groups. 相似文献