Culture of Spirulina platensis in human urine for biomass production and O2 evolution

Attempts were made to culture Spirulina platensis in human urine directly to achieve biomass production and O2 evolution, for potential application to nutrient regeneration and air revitalization in life support system. The culture results showed that Spirulinaplatensis grows successfully in diluted human urine, and yields maximal biomass at urine dilution ratios of 140-240. Accumulation of lipid and decreasing of protein occurred due to N deficiency. O2 release rate of Spirulina platensis in diluted human urine was higher than that in Zarrouk medium.     

Effects of N source and nitrification pretreatment on growth of Arthrospiraplatensis in human urine

Culture of Arthrospira platensis (Spirulina platensis) in human urine was investigated to get valuable biomass. NO3-N was the proper N source, in comparison with other N source, including urea, NH4-N and NO2-N. As a result, aerobic nitrification of human urine was performed, with above 93.6% nitrification percentage finally achieved with total-N (TN) load of 46.52 mg/(L·d), in which Arthrospira platensis was successfully grown. The main compositions of the obtained biomass are close to those in Zarrouk medium. Thus, it is possible to culture Arthrospira platensis in nitrified human urine for food production within bioregenerative life support systems (BLSSs).     

富硒、富锌钝顶螺旋藻培养优化研究

以钝顶螺旋藻为载体,以硒浓度、锌浓度和添加方法为因素,通过单因素和正交试验,确定富锌、富硒钝顶螺旋藻的最佳培养条件.结果表明:在钝顶螺旋藻培养液中加入硒浓度为8 ug/mL、锌浓度为7 ug/mL,加入方法为低水平培养4天(加入浓度的一半)、高水平培养4天(加入剩余一半),为富锌、富硒钝顶螺旋藻的最佳添加浓度和添加方法.     

螺旋藻的生物特性及其应用价值

概述了螺旋藻的生物学特性，并介绍了其营养价值和药用价值，以及在能源和环保方面的潜在应用价值，从而阐明了螺旋藻作为一种新的资源具有很高的开发利用价值。     

钝顶螺旋藻大面积养殖研究

本文报导了钝顶螺旋藻在江西南昌大面积养殖的试验研究资料,为兰藻生产提供依据.     

顿顶螺旋藻完整藻胆体与解离藻胆体光谱特性的比较研究

对钝项螺旋藻完整藻胆体与解离藻胆体吸收光谱进行了比较研究.随着藻胆体的逐渐解离,其吸收光谱表现出如下变化特点:在紫外光区,吸收峰始终位于355nm,尖形峰逐渐变成钝形峰;在红光区,都有很强的吸收,吸收峰呈平顶状,其半带宽逐渐变小;紫外光区与红光区相对吸收强度的比值逐渐变小;四级导数吸收光谱的小峰数目越来越少.室温荧光发射光谱表明,藻胆体在低于0.9mol/L的磷酸缓冲液中变得不稳定,并开始逐渐解离,解离的藻胆体与完整的藻胆体相比,其荧光发射峰波长逐渐变短.     

钝顶螺旋藻藻胆体核心与藻蓝蛋白的重组

用钝项螺旋藻进行了藻胆体核心和藻蓝蛋白重组的研究.在室温荧光发射光谱中,藻胆体核心-藻蓝蛋白重组复合物的荧光发射峰位于663nm,与藻蓝蛋白的室温荧光发射峰643nm和藻蓝蛋白 藻胆体核心混合物(未重组)的室温荧光发射峰648nm相比,荧光发射峰向长波方向发生了移动,并向藻胆体核心室温荧光发射峰664nm逼近.说明藻蓝蛋白与藻胆体核心发生了重组,而且重组效果较好.在液氮温度荧光发射光谱中,其结果也表明藻蓝蛋白与藻胆体核心发生了重组.     

敞开式跑道池螺旋藻大规模培养过程中DO变化规律研究

用自行研制的溶氧(DO)、pH、温度(T)计算机在线检测系统研究敞开式跑道池大规模培养螺旋藻过程中DO三维分布特性和DO、pH、T等参数随时间、天气、搅拌等的变化规律,以期对螺旋藻大规模培养过程的操作条件与生长的关系做进一步地了解,从而为敞开式跑道池螺旋藻大规模培养工艺的综合优化提供实验与理论依据。     

Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against Epstein-Barr virus (EBV) in three Burkitt's lymphoma (BL) cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction (PCR) technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in reducing cell-free EBV DNA (EC(50)<0.01 μg/ml) with a high therapeutic index (>28000). After fractionation by column chromatography, the fraction from Synechococcus elongatus (SEF1) reduced the cell-free EBV DNA most effectively (EC(50)=2.9 μg/ml, therapeutic index>69). Upon further fractionation by high performance liquid chromatography (HPLC), the sub-fraction SEF1'a was most active in reducing the cell-free EBV DNA (EC(50)=1.38 μg/ml, therapeutic index>14.5). This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.     

五种中药水提物清除自由基效应的比较

采用传统方法提取陈皮、葛根、黄芩、槐花米,银杏叶5种中药的水溶性成分,用分光光度法检测水提物对羟自由基（·OH）和超氧阴离子自由基（0i）的清除能力．结果表明,5种中药水提物对·OH和O2-均具有良好的清除作用．比较发现,对于清除羟自由基而言,槐花米的清除效果最佳,当原液稀释2倍时清除率达到了87．40％;对于清除超氧...     

试论韩少功小说创作中的神秘色彩

韩少功前期的小说作品基本上属于传统现实主义作品;后来运用一种新的文学思维,即对人类生命本体和生存方式的关怀,通过关注民间文化,把神秘主义与现实主义相糅合,创作出《爸爸爸》、《女女女》等一批小说;而到了近作——《马桥词典》、《暗示》,又淡出神秘主义,现实主义的色彩得到了加重。     

无汞法测定铁矿石中铁含量的实验改进

对无汞法测定铁矿石中铁含量的实验进行改进,采用SnCl2直接还原无色钨酸钠为蓝色,不需加入TiCl3.以CuSO4作催化剂,利用水中的溶解氧可使钨蓝迅速消失,不用稀释的K2Cr2O7标准溶液至蓝色消失.经实验证明,该方法不仅结果准确度、精确度较高,而且现象易于观察、学生操作简单方便,并降低了实验费用.     

Comparison of the effects of copper and lead on soil microbial biomass carbon and nitrogen in red soil

A laboratory incubation experiment was conducted to study the effect of copper as cupric sulfate (CuSO₄.5H₂O), and lead as lead acetate (Pb (OAc)₂) on the size of the microbial biomass in red soil. The metals were applied, separately at six different levels: Cu at 50, 100, 200, 300, 400, and 600 μg·g⁻¹ soil and Pb at 100, 200, 400, 600, 800, and 1000 μg·g⁻¹ soil. In comparison to uncontaminated soil, the microbial biomass carbon (C_mic) and biomass nitrogen (N_mic) decreased sharply in soils contaminated with Cu and Pb. The microbial biomass C:N ratio in the metal contaminated soil was observed to be considerably higher than that in untreated control. Between the two tested metals, Cu displayed greater biocidal effect on microbial biomass carbon and nitrogen than Pb, showing their relative toxicity in the order: Cu≫Pb. Project (No. 49671050) supported by National Natural Science Foundation of China. Wa’el Mohamad—Former Ph. D. Student of Zhejiang University and is working in Ministry Of Agriculture (P. O. box. 2099), Amman, Jordan.     

大豆根瘤菌AWCS 13－4菌株培养条件的筛选与优化

研究了大豆根瘤菌AWCS13-4菌株在YMA、TY、PA、BSE 4种培养基中的生长情况,并通过单因素和正交试验确定最佳培养条件。结果表明,AWCS13-4菌株在YMA培养基中生长较好,蔗糖为最佳碳源,酵母粉为最佳氮源,最佳p H为7.0,最佳接种量为3%,最佳培养温度为30℃。在单因素的基础上,采用正交试验,对4个因素进行优化,其最佳培养组合为:每升培养基中添加蔗糖10 g,酵母粉3 g,Mg SO4·7H_2O 0.2 g,K2HPO40.5 g,NaCl 0.1 g,CaCl_2·5H_2O 0.05 g,Rh微量元素液4 m L;p H为7.0,接种量为3%,培养温度为30℃。     

语言和文化关系的认知解释与翻译的可译性

在语言和文化关系的传统认识上,有两种相反的观点.这两种观点都是片面的,其不足之处在于它们都把语言和文化看作两个孤立的个体.从人类的认知角度来看,语言和文化都是人类认知能力的外在表现.人类认知的本质决定了语言和文化的性质和它们的关系.语言和文化不是彼此决定的关系,某一语言和文化的结合是历史的、偶然的产物.人类认知能力是无限的,无限的认知能力赋予人类语言以无穷创造性,人类语言可以跨越文化的界限,实现对它文化的理解和接纳.这种对语言和文化的理解构成了翻译可能性的坚实理论基础.     

碰撞与交融：奥尼尔《马克百万》的文化解读

尤金·奥尼尔是美国现代著名剧作家,他广泛吸取了东西方传统文化的精华,又赋予其新内容和新含义。他的《马可百万》集罗曼史、悲剧以及讽刺剧于一体,体现了20世纪以来东西方文化的交流以及它们之间的碰撞与交融。在这部作品中,作者受到的东方恩想和文化的影响,贯穿他对寻找兼具东西方特色的人类灵魂家园的思考。     

贵州彝族毕摩文化传承人问题研究——以贵州省盘县淤泥乡为个案分析

作为彝族核心文化的毕摩文化,由于受教育普及、经济发展、旅游业兴起以及文化资源开发等因素的影响,使得越来越多的年轻人远离传统文化,毕摩文化传承的土壤受到了一定程度的侵蚀,毕摩生存的环境受到威胁,毕摩文化正不断淡化、断层和消失.要使毕摩文化能继续发展和传承,需要在培养学徒、营造生境等方面采取一系列措施,对毕摩文化传承人进行保护.     

一株抗甲醛真菌的培养条件优化

通过单因素及正交试验,对分离得到的一株抗甲醛真菌进行了培养条件的优化．结果表明：牛肉膏对其甲醛抗性的影响较明显;FeSO4·3H2O对菌体浓度的影响较显著．正交实验分析得到最优的培养基为：葡萄糖4％,牛肉膏0．4％,MgSO4·7H2O 0.1％,FeSO4·3H2O 0．01％,KCl 0．05％,pH7．5．在此条件下菌株抗甲醛浓度临界值为6．52mg／mL,较之前提高了94．6％．     

亮毛杜鹃组织培养技术研究初探

本文主要研究了亮毛杜鹃组织培养过程中表面灭菌剂和培养基的选取。实验通过H2O2、Br2(溴水)、NaC1O、HgCl2四种表面灭菌剂对亮毛杜鹃灭菌效果的比较，得到HgCl2的灭菌效果好；同时用四种培养基分别对茎尖、叶片、花芽和带侧芽的茎端作组织培养，得到叶片在1／l0MS培养基中生长状况良好。     

乡土文化：地方时政报道的创新源泉

在媒体竞争日益激烈、电视新闻报道影响力被稀释的背景下,地方电视台的时政新闻要成为主打强档栏目,必须创新。乡土文化正是地方时政新闻报道的创新源泉,其具体策略是：深度理解乡土文化,理念上有效提升时政报道创新意识;有机融合乡土文化,实践上积极探索时政报道创新途径;深入挖掘乡土文化,内涵上充分体现时政报道创新的以人为本;拓展传播资源空间,实现时政报道传播的最大化提升。