Effect of vitamin B12on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice简

The purpose of this study was to investigate the effect of vitamin B₁₂ on palatal development by co-administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and dexamethasone (DEX). We examined the morphological and histological features of the palatal shelf and expression levels of key signaling molecules (transforming growth factor-β3 (TGF-β3) and TGF-β type I receptor (activin receptor-like kinase 5, ALK5)) during palatogenesis among a control group (Group A), TCDD+DEX exposed group (Group B), and TCDD+DEX+vitamin B12 exposed group (Group C). While we failed to find that vitamin B12 decreased the incidence of cleft palate induced by TCDD+DEX treatment, the expression levels of key signaling molecules (TGF-β3 and ALK5) during palatogenesis were significantly modulated. In TCDD+DEX exposed and TCDD+DEX+vitamin B12 exposed groups, palatal shelves could not contact in the midline due to their small sizes. Our results suggest that vitamin B12 may inhibit the expression of some cleft palate inducers such as TGF-β3 and ALK5 in DEX+TCDD exposed mice, which may be beneficial against palatogenesis to some degree, even though we were unable to observe a protective role of vitamin B12 in morphological and histological alterations of palatal shelves induced by DEX and TCDD.     

Sleepy and hostile: The effects of REM sleep deprivation on shock-elicited aggression

Two studies were conducted to assess the effects of rapid eye movement (REM) sleep deprivation on shock-elicited aggression. REM deprivation periods of 0, 24, 48, and 72 h were used in the first study, while 48-, 72-, 96-, and 120-h periods were used in the second study. Both studies indicated that increases in REM deprivation (up to 96 h) resulted in increases in the number of aggressive responses. A decrease was shown by the 120-h group. A drive-energization or motivational-effects model is suggested.     

Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.     

亲和色谱法从融合蛋白GST-IL-6中纯化重组人白细胞介素-6

研究用亲和融合谷胱甘肽 S 转移酶 (GST)的方法纯化重组人白细胞介素 6(IL 6)的发酵和纯化工艺 ,使用含有质粒pHZl818的E .coliJMl0 9在 2XYT培养基中进行发酵表达 ,IL 6表达为与谷胱甘肽 S 转移酶 (GST)融合的融合蛋白GST IL 6.融合蛋白存在可溶的活性蛋白和不可溶的包含体两种形式 ,此包含体无活性且无法复性 ,无法用亲和层析回收 ,通过实验优化摇床发酵的诱导温度和转速 ,以增加可溶融合蛋白的表达 .菌体超声破碎液后 ,上清液用作亲和柱层析 ,可将融合蛋白提纯至 80 00 ,每升发酵液可得 10mg融合蛋白 ,用凝血酶裂解处理 6h ,亲和标志物GST被特异性切除 ,裂解得到的IL 6用离子交换柱层析可纯化至 95 00 ,MTT法测得纯化的IL 6生物学活性为 1.0 2× 10 8IU/mg .     

神经癌细胞PC12对二甲基亚砜耐受剂量的分析

通过观察不同剂量二甲基亚砜(dimethyl sulfoxide DMSO)作用下PC12细胞形态和生长状态的变化,确定PC12细胞对DMSO的耐受剂量.按照一定浓度梯度(V/V)将DMSO加入培养液干预后的24、48和72 h于倒置相差显微镜下观察AO/EB染色前后细胞的形态特征,同时用四甲基偶氮唑蓝(MTT)显色法检测细胞的生存率和计算半数抑制浓度(50%inhibiting concentration,IC50).结果表明:DMSO浓度为14 mL/L时,24 h内90%的PC12细胞生长正常,形态完整,活力与对照组比较无差别,浓度提高到20 mL/L时,24 h时细胞生存率可下降27%,随着作用时间延长,细胞生存率进一步降低;24、48和72 h对应的IC50值分别为31.4、23.9和19.2 mL/L.因此,对PC12细胞而言,24 h内的应用终浓度应低于14 mL/L,干预时间如需延长,DMSO的应用剂量应相应降低.     

Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells

Objective： Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods： Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline （group B）, vitamin C （group C） and vitamin E （group D）. Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline （sample B）, vitamin C （sample C） and vitamin E （sample D）. Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results： In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion： Vitamin C can protect vascular endothelial cells from mannitol-induced injury.     

一种平移不变的形态小波图像融合方法

针对传统的形态小波变换因不具备平移不变性而导致其在图像融合过程中引入伪吉布斯（pseudo-Gibbs）现象和明显的块状效应的问题,本文用循环平移对基于形态小波图像融合方法进行移不变扩展,提出一种有效的平移不变的形态小波图像融合方法。该方法利用形态小波变换对图像进行分解,同时采用循环平移（CycleSpinning）来提高形态小波的平移不变性,有效地抑制融合图像在奇异处的不足。实验结果表明,本文算法的融合图像具有良好的视觉效果及客观评价指标。     

Effects of osmotic stress on antioxidant enzymes activities in leaf discs of P<Subscript>SAG12</Subscript>-<Emphasis Type="Italic">IPT</Emphasis> modified gerbera

Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.     

云烟85种子萌发过程物质消耗研究

以烤烟品种云烟85种子为实验材料,从种子吸水前0h和吸水后的24h,48h,72h,96h,120h,144h,168h分8个时期分析了烟草种子萌发时各种养分的动态变化。结果表明:种子萌发至168h时,种子内可溶性总糖和还原糖含量达最大值,随后可溶性总糖和还原糖含量均维持在一个稳定的水平;种子萌发至24h时,种子内的蛋白质含量开始下降,至168h时达到最低;种子萌发至72h时,种子内的粗脂肪含量开始下降,至168h时达到最低。     

Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid

Objective： To investigate whether vascular endothelial growth factor （VEGF） gene plasmid carried by polytetrafluoroethylene （PTFE） vascular graft materials could transfect endothelial cells （ECs） and promote their growth. Methods： PTFE vascular graft materials carried with pCDI-hVEGF121, pCDI or pEGFP were incubated in Tris-buffer solution and the values of optical density of 260 nm at different time were plotted, then the DNA controlled release curve was made. ECs derived from human umbilical vein were seeded on the pCDI-hVEGF121/pCDI/pEGFP-PTFE materials or tissue culture plates, ECs numbers were counted and VEGF protein concentrations at different time were measured by enzyme-linked immunoadsorbent assay method. Green fluorescent protein （GFP） expression in ECs on pEGFP-PTFE materials was examined with fluorescence mi- croscopy. Results： The controlled release curve showed that the gene released from PTFE materials was rapid within 8 h, then slowed down and that the gene released continuously even after 72 h. At 24, 72 and 120 h, ECs number and proliferation rate of pCDI-hVEGFI21-PTFE materials were higher than those ofpCDI or pEGFP-PTFE materials （P〈0.05）. VEGF protein concentration of pCDI-hVEGF121-PTFE materials was higher than that of pC DI or pEGFP-PTFE materials at 6, 24, 72 and 120 h （P〈0.01）. GFP expression in ECs on the pEGFP-PTFE materials could be detected by fluorescence microscopy. Conclusion： PTFE graft can be used as a carrier of VEGF gene plasmid, VEGF gene carried by PTFE can transfect ECs and promote ECs growth.     

魏晋北朝汉人渐习胡俗之大观

魏晋南北朝是中国历史上的大规模动乱时期,也是我国民族大融合时期.在民族融合过程中,各少数民族积极参与,从多方面给汉族注入了新鲜血液,如服饰器物、饮食、婚姻、乐舞、语言等,极大地丰富了汉族的物质文化和精神文化,形成了具有不同民族特色的多层次文化.     

GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo／reperfusion rat

INTRODUCTION GM1 ganglioside (GM1) is the main kind ofgangliosides in mammalia, and most abundant inbrain tissue (Duchemin et al., 2002). It was reportedthat GM1 could protect cerebral ischemia in vivo andin vitro, one protective mechanism of which is thatGM1 could reduce neural injury induced by toxicityof excitatory amino acid via N-methyl-D-aspartate receptor (NMDAR) (Kharlamov et al., 1993; Simon et al., 1993; Garofalo and Cue…     

图书馆开架借阅的实践与思考--推荐一种适合开架借阅的新型排架法

把闭架借阅和开架借阅做比较,分析了开架借阅沿用传统精确排架法的种种不足,提出了一种适合开架借阅的新型排架法。     

利用农作物废料培养单片黑木耳的技术研究

以几种常见的农业废弃物作为培养原料,对袋料栽培单片黑木耳的最优培养基配方及其培养技术进行了研究。结果表明:在设计的7个试验培养基配方中,以配方7为最佳,即玉米芯40%,稻草15%,棉杆30%,豆秸14%,石灰1%。在初期培养温度25℃,湿度60%,黑暗或弱光;中期培养温度20℃,湿度80%,光照30%-60%;后期培养温度25℃及20℃各12h,其湿度分别对应90%及95%,光照60%和100%,且加强氧气供给,早晚各通风换气30min的培养条件下,菌丝体30d满袋,10d出耳,且木耳量多、色深,一茬产量可达37.18g(干重),产出比(木耳干重除以栽培料干重)可达18.59%。     

Effects of Huangqi and bear bile on recurrent parotitis in children: a new clinical approach

Objective:To evaluate the pharmacological effects of traditional Chinese medicine,bear bile capsule and Huangqi granule,on recurrent parotitis in children.Methods:In this prospective,controlled,and randomized study,a total of 151 young children were divided into three groups:Group A included massaging the children’s parotid region and melting vitamin C in their mouth daily;Group B included swallowing bear bile capsule and Huangqi granule daily;and Group C included massages and vitamin C as prescribed in Group A,and traditional Chinese medicine as prescribed in Group B.Children were treated individually for one month and then a follow-up study was conducted for 1 to 3.5 years.Analysis of variance(ANOVA) and Ridit analysis were employed for statistical analysis.Results:The recurrence rate decreased in every group,but was significantly more in Groups B and C when compared to Group A.The recurrences significantly decreased(P<0.01) in Group B and their recovery rate was as high as 63%,significantly better than those of the other groups(P<0.01).Conclusions:Huangqi and bear bile could be a novel clinical approach for treating recurrent parotitis in children.     

四株细菌降解石油的影响因素研究

实验以B3（Pseudom-onas）、B6（Bacil-lus）、B7（Bacil-lus）、D4（Pseudom-onas）为石油降解菌,通过单因素实验,研究了培养时间、石油浓度、温度、溶氧量、初始pH值等因素对各单菌降解石油性能的影响,确定了各菌最适宜的石油降解条件.四株菌在纱布封口1、60 r/min振荡培养12 d较为适宜,它们适宜的降油石油浓度、温度和初始pH是：B3为4 000 mg/L、32℃、pH8.4,B6为3 000 mg/L、32℃、pH 7.3,B7为4 000 mg/L、26℃、pH7.3,D4为3 000 mg/L、26℃、Ph 7.3.     

辽西区域城市体育文化圈的构建研究

城市体育文化圈是实现区域体育资源空间一体化、提升城市居民综合素质、增强城市核心竞争力的有效途径。立足辽西地区具有明显地域区位优势、特色城市体育品牌、鲜明民俗体育文化、优越户外运动资源、完备体育设施基础和十二届全运会发展契机等特点,提出构建辽西区域体育文化圈的发展策略。旨在促进区域间城市体育跨文化融合,推动区域体育文化产业化发展,实现我国城市体育文化可持续性发展。     

Electrochemical properties of CoFe<Subscript>3</Subscript>Sb<Subscript>12</Subscript> as potential anode material for lithium-ion batteries

A skutterudite-related antimonide, CoFe₃Sb₁₂, was prepared with vacuum melting. XRD analysis showed the material contained Sb, FeSb₂, CoSb₂ and CoSb₃ phases. The electrochemical properties of the ball-milled CoFe₃Sb₁₂−10 wt% graphite composite were studied using pure lithium as the reference electrode. A maximal lithium inserting capacity of about 860 mAh/g was obtained in the first cycle. The reversible capacity of the material was about 560 mAh/g in the first cycle and decreased toca. 320 mAh/g and 250 mAh/g after 10 and 20 cycles respectively.Ex-situ XRD analyses showed that the antimonides in the pristine material were decomposed after the first discharge and that antimony was the active element for lithium to insert into the host material. Project supported by the National Natural Foundation of China (No. 59771032) and the RFDP of the Education Ministry of China (No. 20010335045)     

急性局灶性脑挫裂伤对大鼠脑微循环及神经组织超微结构的影响

目的:探讨大鼠脑损伤后脑微循环障碍、脑缺血及神经组织超微结构的变化规律,为临床改善脑损伤后脑微循环障碍、治疗脑缺血、促进神经功能恢复提供理论依据。方法:采用Feeney's自由落体撞击法建立急性局灶性脑挫裂伤模型,共81只大鼠,随机均分为9组,每组6只行内源性过氧化物酶(EGPO)组织化学染色、脑含水量测定,并进行图象分析。每组3只电镜观察微血管内皮细胞和神经组织超微结构改变。结果:①脑损伤后30min伤区可见出血灶,伤区内无血管染色,伤区周围存在"微无血管区"。"微无血管区"的存在持续至伤后72h。②脑损伤后30min微血管面密度明显下降,伤后48h达到高峰,直到伤后168h才有所恢复,但仍未达到正常水平。③脑损伤后30 min微血管平均光密度明显下降,伤后24h、48h回升,72h再次下降,至168h仍未恢复正常。④脑损伤后30 min,微血管内皮细胞有轻度受损迹象,伤后2h毛细血管腔内有微绒毛形成,伤后6h微绒毛增多。伤后12~72h毛细血管腔明显狭窄。⑤脑损伤后30min,神经细胞超微结构改变不明显,随时间的延长,神经细胞超微结构逐渐恶化,至伤后168h细胞结构完全丧失。结论:EGPO组织化学染色方法能准确反应脑损伤后脑微循环的改变;脑损伤后即发生脑缺血改变,神经细胞、毛细血管内皮细胞的损害继发于脑损伤后的缺血性改变,而脑缺血的发生源于脑损伤后脑微血管结构的破坏和微循环灌注的不足。     

Antimelanogenic effects of <Emphasis Type="Italic">Inula britannica</Emphasis> flower petal extract fermented by <Emphasis Type="Italic">Lactobacillus plantarum</Emphasis> KCCM 11613P

The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening activity, and the contents of total flavonoids and polyphenolics as bioactive compounds were determined. The skin whitening activity using in vitro or ex vivo tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) staining was examined. The total flavonoid content (TFC) was increased by 13.4% after 72 h-fermentation. The viabilities of the B16F10 cells treated with the fermented and non-fermented control extracts were 100.26% and 92.15% at 500 μg/ml, respectively. In addition, the inhibition of tyrosinase activity was increased by the fermented samples from 29.33% to 41.74% following fermentation for up to 72 h. The tyrosinase activity of the untreated control group was increased to 145.69% in B16F10 cells. The results showed that I. britannica fermented by L. plantarum dose-dependently inhibited tyrosinase activity, which was stimulated by α-melanocyte stimulating hormone. These results suggest that lactic fermented I. britannica extracts can be used as effective skin-whitening materials.