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Xiao-liang Li Yue Kang Xiao-yan Zhang Bing-lin Zhu Wei-huan Fang 《Journal of Zhejiang University. Science. B》2012,13(6):465-477
The heat shock cognate protein 70 (Hsc70) is a member of a 70-kDa heat shock protein (HSP70) family that functions as molecular chaperones. In this study, a novel Hsc70 gene from Chinese soft-shelled turtle (Pelodiscus sinensis) (tHsc70) was identified. The tHsc70 full-length complementary DNA (cDNA) is 2 272 bp long with a 1 941-bp open reading frame (ORF) encoding 646 amino acids. Three characteristic signature regions of the HSP70 family, two major domains of an adenosine triphosphate (ATP)/guanosine triphosphate (GTP) binding domain (ABD), and a substrate-binding domain (SBD) were present in the predicted tHsc70 amino acid sequence. The tHsc70 gene was expressed in Escherichia coli BL21 and the expression product reacted with the anti-Hsc70 mouse monoclonal antibody by Western blotting. Homology analysis revealed that tHsc70 shared identity from 53.9% to 87.7% at the nucleotide level, and 49.1% to 99.5% at the amino acid level with the known Hsc70s. Phylogenetic analysis showed that tHsc70 was clustered together with the Hsc70 gene of another reptile species (Alligator mississippiensis). The tHsc70 was expressed in the liver, lung, heart, and skeletal muscle. The expression patterns of tHsc70 messenger RNA (mRNA) differed among different tissues under different durations of heat stress at 40 °C. Adaptation at 25 °C for 1 h after heat stress was also different among tissues and length of heat stress. Irrespective of different profiles of expression under heat stress, tHsc70 may play roles in protecting turtles from thermal stress. 相似文献
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INTRODUCTION It was recognized that when animals are offereda low-nitrogen, high fibre roughage such as rice straw(RS), one of the critical nutrients is fermentable ni-trogen (N) available to rumen microbes (ARC, 1984). The use of urea/molasses blocks is a convenient way for avoiding excessive intake of urea, and will ensure an almost continuous supply of ammonia-N (Preston, 1986). Urea/molasses block feeding yielded positive results in many parts of the world (Kunju, 1986; Hadjipanay… 相似文献
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Xiang Huang Ke-feng Li Jun Du Ran Li 《Journal of Zhejiang University. Science. B》2010,11(10):806-811
Laboratory experiments were conducted to determine the effects of total dissolved gas (TDG) supersaturation on acute lethality
and avoidance responses in juvenile rock carp (Procypris rabaudi Tchang). The juvenile rock carp were exposed to water with different levels of supersaturation (105%, 115%, 120%, 125%, 130%,
135%, 140%, and 145%) and depth of 0.20 m at 25°C for 60 h. Median lethal time (LT50) was used to assess the lethal responses
corresponding to different levels of gas supersaturation. The results show that half of the juvenile rock carp died at the
120%, 125%, 130%, 135%, 140%, and 145% levels of supersaturation, and the LT50 corresponding to different levels of supersaturation
was 18.7, 15.4, 8.2, 6.6, 3.5, and 1.7 h. When the level of supersaturated water is below 115%, the mortality is negligible.
Avoidance responses were observed 5 min after the fish were put into equilibrated water (99%, 0.08 m deep) and water with
different supersaturated levels (105%, 115%, 125%, 135%, and 145%, 0.08 m deep) at 25 °C. The fish exhibited strong avoidance
responses in supersaturated water when the gas supersaturation was above 135%. However, they exhibited an obvious preference
to supersaturated water when the gas supersaturation was below 115%. Thus, the juvenile rock carp can likely survive in water
with a supersaturated level of 115%. 相似文献