灭HP煎剂根治幽门螺杆菌病例72例临床分析

目的:探讨灭幽门螺杆菌(HP)煎剂疗效。方法:采用回顾性方法,在西药治疗基础上服用HP煎剂72例,与同期西药治疗病例88例作副作用及疗效对照。结果:中西治疗组副作用小,HP根治率高。结论:灭HP煎剂有减轻西药副作用,提高HP根治率等作用。     

幽门螺杆菌与消化性溃疡

幽门螺杆菌与消化性溃疡发病有密切关系,引起了医学界的普遍重视。一般认为"无幽门螺杆菌就无溃疡",但目前对其致病因素和致病机理尚不十分清楚。主要认为是该菌丰富的尿素酶分解尿素产氨及其"空泡毒素"引起粘膜损伤,在胃酸作用下受损部位形成溃疡。同时,该菌产生的氨使pH增高,反馈性引起胃泌素分泌增多,使胃酸大量分泌,导致溃疡。基于该菌与消化性溃疡的发病关系如此密切,因此,临床对消化性溃疡在传统抗酸治疗、保护因子应用的基础上加用除菌治疗是十分必要的。     

幽门螺杆菌与消化性溃疡

幽门螺杆菌与消化性溃疡发病有密切关系，引起了医学界的普遍重视。一般认为“无幽门螺杆菌就无溃疡”，但目前对其致病因素和致病机理尚不十分清楚。主要认为是该菌丰富的尿素酶分解尿素产氨及其“空泡毒素”引起粘膜损伤，在胃酸作用下受损部位形成溃疡。同时，该菌产生的氨使pH增高，反馈性引起胃泌素分泌增多，使胃酸大量分泌，导致溃疡。基于该菌与消化性溃疡的发病关系如此密切，因此，临床对消化性溃疡在传统抗酸治疗、保护因子应用的基础上加用除菌治疗是十分必要的。     

幽门螺杆菌异化作用的类型

幽门螺杆菌（Helicobacter pylori,简称Hp）的异化作用类型是什么？有的教师把“厌氧型”作为答案告诉学生。也有的认为是“需氧型”和“好氧型”。那么,Hp异化作用的类型到底是什么？     

幽门螺杆菌与胃十二指肠溃疡

本文通过介绍幽门螺杆菌的细菌学特征、致病机制 ,阐述了幽门螺杆菌感染与胃十二肠溃疡的关系 ,并提出诊断、治疗方案及研究方向     

幽门螺杆菌是什么味道

马歇尔已经记不清那杯培养液到底是什么味道。但正是当年的一饮而下,掀起了一场医学风暴,使不可计数的患者得到了更加科学的救治。     

幽门螺旋杆菌感染与耳鼻咽喉头颈外科疾病关系研究综述

幽门螺旋杆菌与慢性胃炎及消化性溃疡密切相关,但最近研究发现与某些耳鼻咽喉头颈外科疾病亦有一定关系。本文从阐述幽门螺旋杆菌的生物学性状、诊断技术、流行病学及抗幽门螺旋杆菌治疗入手,综述了幽门螺旋杆菌与耳鼻咽喉头颈外科疾病的关系,为从系统因素出发研究耳鼻咽喉头颈外科疾病提供新思路。     

慢性幽门螺杆菌感染与口腔相关疾病研究进展

幽门螺杆菌是多种胃肠道疾病的主要致病因素,但近年来的研究发现与某些口腔疾病亦有一定关系。本文从阐述幽门螺杆菌(Hp)的生物学特性、流行病学、口腔中Hp检测、口腔Hp与胃内Hp关系、口腔Hp与口腔疾病,五个方面进行综述,揭示幽门螺杆菌感染与口腔疾病的关系。     

草履虫的采集分离、培养及保存新方法研究概述

对动物学实验教学中的草履虫材料的采集分离、培养方法进行了探讨,同时对草履虫进行了保存方法的研究,随时为高等院校及中学生物学实验课常用的实验材料进行及时培养,为教学科研提供帮助.     

三联疗法结合微生态制剂治疗幽门螺杆菌感染的临床研究

目的：探讨微生态制剂联合兰索拉唑、克拉霉素片、阿莫西林胶囊在幽门螺杆菌感染治疗中的疗效及安全性。方法：将118例慢性胃炎或消化性溃疡且经快速尿素酶试验、病理切片染色镜检、13C-尿素酶呼气试验检测幽门螺杆菌任何两项阳性患者随机分为联合治疗组60例,常规三联方根除治疗（兰索拉唑＋克拉霉素＋阿莫西林胶囊）加0．35g双歧杆菌片0．7g2次／d,口服,与抗菌药物间隔2h服用,疗程14d。对照组58例,常规三联方根除治疗（兰索拉唑＋克拉霉素＋阿莫西林胶囊）不使用双歧杆菌,疗程14d。治疗结束4周后复查”C－尿素酶呼气试验。比较两组根除率及不良反应发生率。结果：联合治疗组根除率80％,对照组根除率77．5％,两组间差异无统计学意义（P〉．05）,但是联合治疗组不良反应发生率6．7％与对照组不良反应发生率22．4％比较差异具有统计学意义（P〈0．05）。结论：常规三联疗法结合双歧杆菌片在幽门螺杆菌感染治疗中不良作用少,患者依从性较好,值得临床推广和应用。     

Study of serum Helicobacter pylori soluble antigen

Objective: to explore a new serological method for detecting Helicobac ter pylori ( H. pylori ) infection. Methods: Serum soluble antigen of H. p ylor i was detected by using avidin-biotin ELISA technique to evaluate the status of H. pylori infection and for comparison with rapid urease test ( RUT ), histo logi c examination and serology. Results: The sensitivity, specificity, positive pred ictive value and negative predictive value were 77.46%, 91.07%, 91.67% a nd 76.12 %, respectively. The prevalence rate of serum H. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by 14 C-UBT met hods ( P>0.05 ). Conclusions: The detection of serum H. pylori solub le antigen( HpSAg) could be used as a new serological method which is accurate, and convenie nt, not affected by the memorizing reaction of serum antibody; is more sensitive , m ore specific and suitable for clinical diagnosis, and evaluation of eradication and for follow-up of H. pylori as well as for detection in children and pre gnant women.     

四联疗法根治幽门螺旋杆菌疗效观察

目的探讨以四联疗法作为一线方案治疗幽门螺旋杆菌（HP）阳性的胃病患者临床疗效,并与传统三联疗法比较,找出更有效的根治HP的办法。方法将105例HP阳性患者随机分为2组,对照组48例患者给予传统三联疗法,而治疗组57例患者给予含铋剂的四联疗法,治疗结束后4周复查2组患者的HP感染。结果对照组HP根治率为77.08%,治疗组为92.98%,两组结果经统计学检验有显著性差异（P〈0.05）。两组的副反应无显著性差异（P〉0.05）。结论四联疗法根治幽门螺杆菌感染较三联疗法疗效更显著,且副作用小,费用合理,可作为初治方案进一步推广。     

幽门螺杆菌感染蒙古沙土鼠疾病模型的建立

利用蒙古沙土鼠建立幽门螺杆菌（Helicobacter pylori,Hp）NCTC 11637株胃内感染模型。感染方法有两种,方法Ⅰ是沙土鼠禁食18h后用50%酒精0.5mL预处理,6h后接种Hp,间隔12h、24h分别再次接种。方法Ⅱ的接种方法同方法Ⅰ,但接种后每日口服雷尼替丁（5m g/kg体重）。胃内接种H p后,于第7天、15天、35天、45天分别用Hp抗原酶联免疫诊断试剂盒、细菌培养和胃组织切片的方法观察沙土鼠胃内细菌感染情况和胃病理组织学变化。结果表明,给沙土鼠胃内灌服酒精以损伤胃黏膜并每日口服雷尼替丁,在人工接种35天后,胃内见有多量Hp生长,胃组织病变与Hp自然感染的病例相似。     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1.     

Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori

Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein of H. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer ) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gastritis 86.7% (13/15),peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327, P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (rs=0.442, P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases.     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｔｈｅｆｉｒｓｔｓｕｃｃｅｓｓｆｕｌｉｓｏｌａｔｉｏｎｏｆＨ .ｐｙ ｌｏｒｉｉｎ 1 982 ,Ｈ .ｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎｈａｓｂｅｅｎｆｏｕｎｄｔｏｂｅａｓｓｏｃｉａｔｅｄｗｉｔｈｖａｒｉｏｕｓｄｉｓｅａｓｅｓｉｎｃｌｕｄｉｎｇｃｈｒｏｎｉｃｇａｓｔｒｉｔｉｓ,ｐｅｐｔｉｃｕｌｃｅｒｄｉｓｅａｓｅｓ(Ｄｏｏｌｅｙｅｔａｌ.,1 989;Ｇｒａｈａｍｅｔａｌ.,1 992 ) ,ａｎｄｇａｓｔｒｉｃｎｅｏｐｌａｓｍｓ(Ｂａｙｅｒｄｏｒｆｆｅｒｅｔａｌ.,1 995) .Ｉｔｗａｓｓｕｇｇｅｓｔ…     

Purification and relationship with gastric disease of a 130 kDa (CagA)protein of Helicobacter pylori

ＩＮＴＲＯＤＵＣＴＩＯＮＨｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎｈａｄｂｅｅｎｒｅｃｏｇ ｎｉｚｅｄａｓａｎｉｍｐｏｒｔａｎｔｃａｕｓａｔｉｖｅｆａｃｔｏｒｉｎｃｈｒｏｎｉｃｇａｓｔｒｉｔｉｓａｎｄｐｅｐｔｉｃｕｌｃｅｒｄｉｓｅａｓｅ (ＰＵＤ     

基于臭氧技术的渔获物船舱保鲜装置的研究

介绍了一种采用臭氧处理的渔获物船舱保鲜装置的结构组成、工作过程与操作控制、操作使用有关参数要求及其在无制冷设备的渔船舱储加冰保鲜渔获物杀菌保鲜上的应用。使用该装置，舱储加冰保鲜渔获物的保鲜期可由原来的7-12天延长到13～15天，且能抑制硫化氢的产生，也不存在二次污染，保证了食品安全和生产作业安全，在渔船上有较广泛的适用性和很好的实效性。