热休克蛋白的研究进展及其应用

在不利的环境中，各种有机体都有其共同对应的分子反应，即正常基因的表达抑制和一组特殊基因－热休克基因的激活和表达，并导致热休克蛋白的大量产生。热休克蛋白主要作为分了伴侣而参与蛋白的折叠、转运及组装等过程，并能恢复或加速清除细胞内已变性的蛋白质而稳定细胞结构，使细胞产生热耐受。随着对热休克蛋白研究的不断深入，其在生物工程、医学和遗传育种等方面的应用前景十分广阔。     

昆虫抗细菌蛋白质的研究进展

从不同的昆虫中已分离子多种抗细菌蛋白质,这些抗细菌蛋白可分为杀菌肽,防御素、攻击素、富含甘氨酸、富含脯氨酸和其它类型６类。本文综述了这些抗细菌蛋白质的基因结构和基因表达以及抗菌机理的研究进展。     

Monitoring Coagulation Proteins During Progression of Liver Disease

This work was designated to monitor the coagulation abnormalities associated with the gradual progression of liver diseases. The study included fifty patients; forty were diagnosed with liver cirrhosis with different stages categorized according to the Childs-Pugh classification and another ten patients were diagnosed with hepatocellular carcinoma (HCC). Haemostatic variables including fibrinogen (FI), calcium (FIV), transglutaminase (FXIII), prothrombin time (PT) and platelet count were estimated in patients and compared with the baseline levels of healthy subjects (n = 10). The results demonstrated that the fibrinogen level was progressively decreased, whereas PT was progressively prolonged in Child A, Child B and Child C groups. The maximum deterioration was observed in HCC patients. Calcium significantly increased in mild (Child A) and moderate (Child B) but not in Child C cirrhosis and HCC patients. FXIII level did not show any significant changes in cirrhotic patients compared to healthy group. Some of the haemostatic variables we investigated were correlated with serum albumin and bilirubin but not with aminotransferases (ALT and AST). The results indicated that the haemostatic abnormalities in fibrinogen, calcium and PT (but not FXIII) were deteriorated in parallel with the gradual regression of the constitutional function of liver.     

Comparison of Urinary Total Proteins by Four Different Methods

The total proteins in human urine have been compared by sulfosalicylic acid, sulfosalicylic acid with sodium sulphate and trichloroacetic acid methods with pyrogallol red molybdate method as there are no studies found quantifying imprecision and bias components. Fresh urine of 36 patients was analyzed by four methods. Imprecision and inaccuracy were determined by repeated analysis and method comparison studies using correlation plots, Bland and Altman, and Passing and Bablok regression analyses respectively. The coefficient of variation was 5.07 % for pyrogallol red molybdate; 6.84 % for sulfosalicylic acid; 3.97 % for sulfosalicylic acid with sodium sulphate and 5.93 % for trichloroacetic acid methods. Bland and Altman analysis showed a bias of 5.8, 1.7 and ?5.4 for pyrogallol red molybdate versus sulfosalicylic acid, sulfosalicylic acid with sodium sulphate and trichloroacetic acid methods respectively. Passing and Bablok regression revealed a constant bias for pyrogallol red molybdate versus all turbidimetric methods but a proportional bias only with trichloroacetic acid method. Sulfosalicylic acid with sodium sulphate method is preferred to sulfosalicylic acid and trichloroacetic acid methods.     

三种百合线状病毒的外壳蛋白抗血清制备

设计3对表达引物分别扩增侵染了百合无症病毒（lily symptomless virus,LSV）．百合斑驳病毒（lily mottle virus,LMoV）和百合X病毒（lily virus X,LVX）的外壳蛋白基因,并在大肠杆菌BL21（DE3）plysS中原核表达。目的蛋白切胶后免疫小鼠制备多克隆抗体。Western blot检测表明3种病毒抗血清分别与目的蛋白起特异性反应．但与阴性对照无反应。3种病毒间无交叉血清学反应。对田间栽种的百合样品间接ELISA检测表明．LMoV和LSV均有发生,但没有检测到LVX。     

New Technique Developed to Identify Clusters of Proteins on the Plasma Membrane of Immune Cells

<正>When our bodies are under attack from foreign organisms,such as bacteria and viruses,our immune system orchestrates a complex fight-back involving many separate parts.One important component of this response is a type of cell called the B-lymphocyte–it is this cell that is at the forefront of our     

Structural and Functional Studies of Ribosome-inactivating Proteins and Ribosomal RNA

A plant's ribosome-inactivating proteins (RIPs) are a group of toxic proteins. Theoretically, they can be employed as a tool enzyme in the exploration of the structure and function of the ribosomal RNA; in practical application, they can be used as an insecticide in agriculture, for preparation of immuno-toxic protein to kill cancer cells or against viral infection in medicine.     

分子水平的非随机进化──相互作用蛋白质间的达尔文选择

通过分析13种生物120余种蛋白质的分子进化,首次揭示:配基-受体、蛋白酶-酶抑制剂、亚基-亚基等相互作用蛋白质间在纵向进化(物种间)、横向进化(不同功能蛋白质间)中均存在协同进化的规律.此结果表明,相互作用蛋白质其分子进化普遍为非随机过程,由于其相互作用为功能发挥所必需,因而彼此间以典型的达尔文选择方式进化.本文结果既弥补了中性理论中分子进化与功能方式无关的不足,亦指出达尔文选择适用于分子进化。     

CAS Scientists Succeed in Expressing Key Proteins in SARS Virus

In cooperation with collea]gesfrom other labs, researchers ofthe CAS Shanghai Institutes ofMateria MedicaSIMMand theCAS Insti0tute of Biochemistry andCell Biol[(o)(gy\()IBCB) have madeprogress] in gene cloning], proteinexpression, structural analysis, anddrug design in regard to SARS virus.This was announced May by PeiGang, dire0(c)]tor of the CAS Sha[(n)(g)]-hai Insti0t]utes for Biol[(o)(g)]i[(cal Scien[(c)0(e)]s,parent nstit]u[(tion of the twoinstitutes.The scienti]sts have ful…     

A biochemical study on the effect of Riboflavin on cataractous rat lenses. II Proteins and Glutathione

Proteins and glutathione (GSH) levels were estimated in the experimental groups. Levels of both proteins and glutathione in the control and the treated groups were found to be significantly decreased compared to the normal group. The results show that supplementation of riboflavin has beneficial effect by alleviating the oxidant injuries to GSH and proteins, which are involved in the maintenance of transparency and protection of thiol groups of the lens. Thus, riboflavin has an active role in the lens metabolism and has a potential to improve the metabolic activity of the cataractous lenses.     

三种三尖杉属植物越冬期光合器的功能和膜蛋白成分的比较研究

本文对三尖杉属中三种植物即：三尖杉Cephalotaxus fortunei，粗榧C.sinensis 和柱   冠日本粗榧C.harringtonia cv．Fastigiata越冬期的光合器功能进行了研究。研究结果表明   这三种常绿三尖杉植物越冬时叶绿体的光合放氧活性受到抑制，但光系统II中心的活性和镁   离子调节光能分配的功能存在。说明其越冬特性与松柏科植物类似。     叶绿体膜的低温荧光测定结果表明三种植物的F685,F695和F735的比率各不相同。用SDS—   聚丙烯酰胺凝胶电泳分析叶绿体膜蛋白，发现柱冠日本粗榧的多肽数与三尖杉和粗榧有明显差异。以上特征可能有助于分类或系统位置的研究。