Computational investigations of the mixing performance inside liquid slugs generated by a microfluidic T-junction

Droplet-based microfluidics has gained extensive research interest as it overcomes several challenges confronted by conventional single-phase microfluidics. The mixing performance inside droplets/slugs is critical in many applications such as advanced material syntheses and in situ kinetic measurements. In order to understand the effects of operating conditions on the mixing performance inside liquid slugs generated by a microfluidic T-junction, we have adopted the volume of fluid method coupled with the species transport model to study and quantify the mixing efficiencies inside slugs. Our simulation results demonstrate that an efficient mixing process is achieved by the intimate collaboration of the twirling effect and the recirculating flow. Only if the reagents are distributed transversely by the twirling effect, the recirculating flow can bring in convection mechanism thus facilitating mixing. By comparing the mixing performance inside slugs at various operating conditions, we find that slug size plays the key role in influencing the mixing performance as it determines the amount of fluid to be distributed by the twirling effect. For the cases where short slugs are generated, the mixing process is governed by the fast convection mechanism because the twirling effect can distribute the fluid to the flow path of the recirculating flow effectively. For cases with long slugs, the mixing process is dominated by the slow diffusion mechanism since the twirling effect is insufficient to distribute the large amount of fluid. In addition, our results show that increasing the operating velocity has limited effects on improving the mixing performance. This study provides the insight of the mixing process and may benefit the design and operations of droplet-based microfluidics.     

Novel method of generating water-in-oil(W∕O) droplets in a microchannel with grooved walls

We present a novel method of generating and retrieving droplets stored in microfluidic grooves or cavity structures. First we designed and fabricated polydimethylsiloxane microchannels with grooves on the walls and then produced a two-phase flow of oil and aqueous phases to form aqueous phase droplets in an oil state. We propose the following three mechanisms of droplet generation: the contact line on the groove wall continues moving along the wall and descends to the bottom of the cavity, confining the aqueous phase in the cavity; once the interface between the oil and aqueous phases moves into the cavity, the interface contacts the top of the neighboring groove; and a spherical droplet forms at the corner in the cavity due to surface tension. The viscosity of the oil phase and the surface tension of the interface determine whether a droplet can be generated. Then, we could adjust the velocity of the interface and the aspect ratio of the cavity to achieve the optimal conditions for generating the single droplet. We observed that the largest droplet is stably generated without a daughter droplet at typical values of free-stream velocity (10 μl∕min) and groove pitch 110 μm for all three cases with different oil phases (20, 50, and 84 cP). This technique is expected to serve as a platform for droplet-based reaction systems, particularly with regard to monitoring cell behavior, in vitro expression, and possibly even micropolymerase chain reaction chambers.     

Temperature-induced droplet coalescence in microchannels

This paper reports a technique for temperature-induced merging of droplets in a microchannel. The multiphase system consists of water droplet and oil as the dispersed phase and the carrying continuous phase. A resistive heater provides heating in a rectangular merging chamber. The temperature of the chamber is controlled by the voltage applied to the heater. The merging process of two neighboring droplets was investigated with different applied voltage, flow rate ratio between water and oil and total flowrate. Merging is found to be effective at high flow rate ratio, high temperature, and low total flowrate. The presented technique could be used for merging and mixing in droplet-based lab-on-a-chip platforms.     

Microfluidic channel structures speed up mixing of multiple emulsions by a factor of ten

We present a novel use for channel structures in microfluidic devices, whereby two two-phase emulsions, one created on-chip, the other off-chip, are rapidly mixed with each other in order to allow for the coalescence of one emulsion with the other. This approach has been motivated by the difficulty in introducing aqueous cross linking agents into droplets by utilising conventional approaches. These conventional approaches include continuous introduction of the different aqueous reagents before droplet formation or alternatively formation of individual droplets of each reagent and subsequent droplet merging later in the microfluidic device. We show that our approach can decrease the mixing time for these fluidic systems by a factor greater than 10 times when compared to a standard microfluidic channel without structures, thereby also allowing for additional reaction time within the microfluidic device. This method shows an application for microfluidic channel structures not before demonstrated, also demonstrating an alternative method for introducing reagents such as cross linkers which link polymer chains to form particles, and provides an example where enzymes are immobilized in monodisperse particles.     

A polymeric micro-optical interface for flow monitoring in biomicrofluidics

We describe design and miniaturization of a polymeric optical interface for flow monitoring in biomicrofluidics applications based on polydimethylsiloxane technology, providing optical transparency and compatibility with biological tissues. Design and ray tracing simulation are presented as well as device realization and optical analysis of flow dynamics in microscopic blood vessels. Optics characterization of this polymeric microinterface in dynamic experimental conditions provides a proof of concept for the application of the device to two-phase flow monitoring in both in vitro experiments and in vivo microcirculation investigations. This technology supports the study of in vitro and in vivo microfluidic systems. It yields simultaneous optical measurements, allowing for continuous monitoring of flow. This development, integrating a well-known and widely used optical flow monitoring systems, provides a disposable interface between live mammalian tissues and microfluidic devices making them accessible to detection∕processing technology, in support or replacing standard intravital microscopy.     

Rapid separation of bacteriorhodopsin using a laminar-flow extraction system in a microfluidic device

A protein separation technology using the microfluidic device was developed for the more rapid and effective analysis of target protein. This microfluidic separation system was carried out using the aqueous two-phase system (ATPS) and the ionic liquid two-phase system (ILTPS) for purification method of the protein sample, and the three-flow desalting system was used for the removal of salts from the sucrose-rich sample. Partitioning of the protein sample was observed in ATPS or ILTPS with the various pHs. The microdialysis system was applied to remove small molecules, such as sucrose and salts in the microfluidic channel with the different flow rates of buffer phase. A complex purification method, which combines microdialysis and ATPS or ILTPS, was carried out for the effective purification of bacteriorhodopsin (BR) from the purple membrane of Halobacterium salinarium, which was then analyzed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and matrix-assisted laser desorption∕ionization time-of-flight. Furthermore, we were able to make a stable three-phase flow controlling the flow rate in the microfluidic channel. Our complex purification methods were successful in purifying and recovering the BR to its required value.     

Polymer stretch in two-phase microfluidics: Effect of wall wettability

Polymer stretching in two-phase microfluidics is investigated by dissipative particle dynamics. The flow patterns can be controlled by wall wettability, flowrate ratio between two phases, and Reynolds number (Re). For neutral and partially wettable walls, segmented flows are formed and polymer stretching can be controlled by Re and segment length. At high Re, stratified flows are observed and the extension ratio can be tuned by the flowrate ratio. For nonwettable walls, slug flows are formed and polymer stretching can be controlled by Re and slug length. At high Re or flowrate ratio, annular flows are observed and high extension ratio can be easily attained.     

Microfluidic conformal coating of non-spherical magnetic particles

We present the conformal coating of non-spherical magnetic particles in a co-laminar flow microfluidic system. Whereas in the previous reports spherical particles had been coated with thin films that formed spheres around the particles; in this article, we show the coating of non-spherical particles with coating layers that are approximately uniform in thickness. The novelty of our work is that while liquid-liquid interfacial tension tends to minimize the surface area of interfaces—for example, to form spherical droplets that encapsulate spherical particles—in our experiments, the thin film that coats non-spherical particles has a non-minimal interfacial area. We first make bullet-shaped magnetic microparticles using a stop-flow lithography method that was previously demonstrated. We then suspend the bullet-shaped microparticles in an aqueous solution and flow the particle suspension with a co-flow of a non-aqueous mixture. A magnetic field gradient from a permanent magnet pulls the microparticles in the transverse direction to the fluid flow, until the particles reach the interface between the immiscible fluids. We observe that upon crossing the oil-water interface, the microparticles become coated by a thin film of the aqueous fluid. When we increase the two-fluid interfacial tension by reducing surfactant concentration, we observe that the particles become trapped at the interface, and we use this observation to extract an approximate magnetic susceptibility of the manufactured non-spherical microparticles. Finally, using fluorescence imaging, we confirm the uniformity of the thin film coating along the entire curved surface of the bullet-shaped particles. To the best of our knowledge, this is the first demonstration of conformal coating of non-spherical particles using microfluidics.     

高温相变材料在重力热管外表面凝固的偶合计算

利用高温相变材料储热，同时用重力热管作为能量传输元件这一高效偶合装置在生产和生活中有广阔的应用前景。本文在实验基础上建立一个考虑自然对流影响的相变传热与热管传热相偶合的传热模型，并对该模型进行数值计算，通过与实验结果进行的对比，证实所建立的模型与所采用的计算方法均是合理可行的。     

利用三维VOF模型模拟消能池的淹没水跃

采用两相流理论中三维相间界面计算模型——VOF模型模拟计算水流进入急流槽消能池发生淹没式水跃的过程,跟踪自由水面的变化。模拟结果表明VOF模型能较为准确地实现自由水面的跟踪模拟。     

Droplet formation and shrinking in aqueous two-phase systems using a membrane emulsification method

Using a membrane emulsification method based on porous hollow-fiber membranes in combination with an aqueous two-phase system (ATPS), we are able to produce “water-in-water” droplets with narrow-dispersed size distributions. The equilibrium phases of the aqueous two-phase system polyethylene glycol-dipotassium hydrogen phosphate are used for this purpose. The droplet diameter of a given fluid system is determined by the flow rates of the continuous and disperse phase as well as the hollow fiber dimensions. When diluting the disperse phase and thus moving the ATPS system out of equilibrium, the droplet size can be further reduced in comparison to the equilibrium case. Generally, droplets formed with this method have diameters 20%–60% larger than the inner hollow fiber diameter. The new strategy of diluting the disperse phase allows the production of droplet diameter below the inner diameter of the membrane.     

混合层中四涡合并过程的数值研究

用拟谱方法对混合层四涡合并的规律进行了数值研究。分析了其波和次谐波之间相位差对涡合并过程的影响,并引入旋转点和拉伸点的概念对种影响进行了解释。计算结果表明：四涡的合并存在６种形式,因此,可以通过施加多级次谐波并改变它们之间的相位差主动控制拟序涡的发展和演变。     

Application of “stability index” to assess the stability of multi-machine systems

A new stability index is presented in this paper which may easily be calculated using the information obtained from the load flow study and the fault location and time. No information from previous transient stability studies of the system is required.The conventional methods of studying transient stability require long computing time whereas the use of stability index is a very fast method of assessing the system's stability. Application of the index provides a very quick insight into the behaviour of the generator under a three-phase fault and identifies the critical situations.Results of the calculation of this index for a sample power system are included and within a reasonable accuracy agree with the results of a full transient stability analysis.     

A two phase investment game for competitive opinion dynamics in social networks

We propose a setting for two-phase opinion dynamics in social networks, where a node’s final opinion in the first phase acts as its initial biased opinion in the second phase. In this setting, we study the problem of two camps aiming to maximize adoption of their respective opinions, by strategically investing on nodes in the two phases. A node’s initial opinion in the second phase naturally plays a key role in determining the final opinion of that node, and hence also of other nodes in the network due to its influence on them. However, more importantly, this bias also determines the effectiveness of a camp’s investment on that node in the second phase. In order to formalize this two-phase investment setting, we propose an extension of Friedkin–Johnsen model, and hence formulate the utility functions of the camps. We arrive at a decision parameter which can be interpreted as two-phase Katz centrality. There is a natural tradeoff while splitting the available budget between the two phases. A lower investment in the first phase results in worse initial biases in the network for the second phase. On the other hand, a higher investment in the first phase spares a lower available budget for the second phase, resulting in an inability to fully harness the influenced biases. We first analyze the non-competitive case where only one camp invests, for which we present a polynomial time algorithm for determining an optimal way to split the camp’s budget between the two phases. We then analyze the case of competing camps, where we show the existence of Nash equilibrium and that it can be computed in polynomial time under reasonable assumptions. We conclude our study with simulations on real-world network datasets, in order to quantify the effects of the initial biases and the weightage attributed by nodes to their initial biases, as well as that of a camp deviating from its equilibrium strategy. Our main conclusion is that, if nodes attribute high weightage to their initial biases, it is advantageous to have a high investment in the first phase, so as to effectively influence the biases to be harnessed in the second phase.     

Mixing enhancement in microfluidic channel with a constriction under periodic electro-osmotic flow

We present a new approach to enhance mixing in T-type micromixers by introducing a constriction in the microchannel under periodic electro-osmotic flow. Two sinusoidal ac electric fields with 180° phase difference and similar dc bias are applied at the two inlets. The out of phase ac electric field induces oscillation of fluid interface at the junction of the two inlet channels and the constriction. Due to the constriction introduced at the junction, fluids from these two inlets form alternative plugs at the constricted channel. These plugs of fluids radiate downstream from the constriction into the large channel and form alternate thin crescent-shaped layers of fluids. These crescent-shaped layers of fluids increase tremendously the contact surface area between the two streams of fluid and thus enhance significantly the mixing efficiency. Experimental results and mixing mechanism analysis show that amplitude and frequency of the ac electric field and the length of the constriction govern the mixing efficiency.     

用ERDAS IMAGINE空间建模工具实现IHS变换融合

ERDAS IMAGINE是美国ERDAS公司开发的遥感图像处理系统,为遥感及相关应用领域的用户提供了内容丰富、功能强大的图像处理工具。特别是给用户提供的一个空间建模工具,借用这个工具可以根据用户的需要,定制并实现特定的图像处理的操作功能。IHS变换属于色彩空间的变换,由于灵活实用的优点而被广泛使用,成为图像融合技术的一个成熟的方法。运用ERDAS IMAGINE提供的空间建模工具可以简单准确的实现图像的IHS变换融合．并有广泛的应用价值。     

Programmed sample delivery on a pressurized paper

This paper reports a method to control the fluid flow in paper-based microfluidic devices simply by pressing over the channel surface of paper, thereby decreasing the pore size and permeability of a non-woven polypropylene sheet. As a result, fluid resistance is increased in the pressed region and causes flow rate to decrease. We characterize the decrease of flow rate with respect to different amounts of pressure applied, and up to 740% decrease in flow velocity was achieved. In addition, we demonstrate flow rate control in a Y-shaped merging paper and sequential delivery of multiple color dyes in a three-branched paper. Furthermore, sequential delivery of multiple fluid samples is performed to demonstrate its application in multi-step colorimetric immunoassay, which shows a 4.3-fold signal increase via enhancement step.     

Examining the effectiveness of real-time query expansion

Interactive query expansion (IQE) (c.f. [Efthimiadis, E. N. (1996). Query expansion. Annual Review of Information Systems and Technology, 31, 121–187]) is a potentially useful technique to help searchers formulate improved query statements, and ultimately retrieve better search results. However, IQE is seldom used in operational settings. Two possible explanations for this are that IQE is generally not integrated into searchers’ established information-seeking behaviors (e.g., examining lists of documents), and it may not be offered at a time in the search when it is needed most (i.e., during the initial query formulation). These challenges can be addressed by coupling IQE more closely with familiar search activities, rather than as a separate functionality that searchers must learn. In this article we introduce and evaluate a variant of IQE known as Real-Time Query Expansion (RTQE). As a searcher enters their query in a text box at the interface, RTQE provides a list of suggested additional query terms, in effect offering query expansion options while the query is formulated. To investigate how the technique is used – and when it may be useful – we conducted a user study comparing three search interfaces: a baseline interface with no query expansion support; an interface that provides expansion options during query entry, and a third interface that provides options after queries have been submitted to a search system. The results show that offering RTQE leads to better quality initial queries, more engagement in the search, and an increase in the uptake of query expansion. However, the results also imply that care must be taken when implementing RTQE interactively. Our findings have broad implications for how IQE should be offered, and form part of our research on the development of techniques to support the increased use of query expansion.     

A numerical study on the dynamics of droplet formation in a microfluidic double T-junction

In this study, droplet formations in microfluidic double T-junctions (MFDTD) are investigated based on a two-dimensional numerical model with volume of fluid method. Parametric ranges for generating alternating droplet formation (ADF) are identified. A physical background responsible for the ADF is suggested by analyzing the dynamical stability of flow system. Since the phase discrepancy between dispersed flows is mainly caused by non-symmetrical breaking of merging droplet, merging regime becomes the alternating regime at appropriate conditions. In addition, the effects of channel geometries on droplet formation are studied in terms of relative channel width. The predicted results show that the ADF region is shifted toward lower capillary numbers when channel width ratio is less than unity. The alternating droplet size increases with the increase of channel width ratio. When this ratio reaches unity, alternating droplets can be formed at very high water fraction (wf = 0.8). The droplet formation in MFDTD depends significantly on the viscosity ratio, and the droplet size in ADF decreases with the increase of the viscosity ratio. The understanding of underlying physics of the ADF phenomenon is useful for many applications, including nanoparticle synthesis with different concentrations, hydrogel bead generation, and cell transplantation in biomedical therapy.     

Enhanced H-filter based on F?hr?us-Lindqvist effect for efficient and robust dialysis without membrane

A novel microfluidic device for highly efficient and robust dialysis without membrane is highly desired for the development of portable or wearable microdialyzer. Here we report an enhanced H-filter with pillar array based on Fåhræus-Lindqvist effect (F-L effect) for highly efficient and robust membraneless dialysis of simplified blood for the first time. The H-filter employs two fluids laminarly flowing in the microchannel for continuously membraneless dialysis. With pillar array in the microchannel, the two laminar flows, with one containing blood cells and small molecules and another containing dialyzate solution, can form a cell-free layer at the interface as selective zones for separation. This provides enhanced mixing yet extremely low shear for extraction of small molecules from the blood-cell-containing flow into the dialyzate flow, resulting in robust separation with reduced cell loss and improved efficiency. We demonstrate this by first using Chlorella pyrenoidosa as model cells to quantitatively study the separation performances, and then using simplified human blood for dialysis. The advanced H-filter, with highly efficient and robust performance for membraneless dialysis, shows great potential as promising candidate for rapid blood analysis/separation, and as fundamental structure for portable dialyzer.