水提树脂吸附法提取分离柿叶黄酮

为了从柿叶中提取具有降血压功能及促进植物生长作用的柿叶黄酮,通过提取溶剂和吸附树脂的筛选,选用ｐＨ为１３的水作溶剂,成本低,工艺安全,简单。然后用ＹＦ－Ｉ树脂吸附,乙醇洗脱。提取获得的粗黄酮,其组份通过纸色谱和紫外光谱比较与有机溶剂提取法基本相同,且具有生物活性。     

一点红黄酮含量测定研究综述

通过查阅大量文献可知,国内对于一点红黄酮的提取及抗肿瘤研究尚不完善。目前一点红黄酮研究方法有:超声波提取法、大孔树脂柱吸附法、连续回流提取法、高效液相色谱法、超临界萃取、单因素实验分析法等。本文拟采用酸水解法预处理一点红样品,超声波法提取一点红黄酮,使用高效液相色谱法分离纯化、测定黄酮含量,并采用核磁共振技术鉴定黄酮结构式。为更好开发利用海南黎药一点红黄酮的药理作用,使其药理作用得到最大化,需对一点红黄酮进行深入的理论研究。     

肾叶细辛总黄酮的分离纯化工艺研究

本研究通过对大孔吸附树脂的简单介绍,讨论了几种常见化合物的研究进展。以动态和静态吸附率及解析率为指标,通过考察AB-8、LAS-10、D101和HPD-100四种大孔树脂对肾叶细辛总黄酮的吸附和解吸性能,从中筛选适用的大孔树脂类型,以期为深入挖掘肾叶细辛黄酮的分离纯化工艺提供新思路。     

大孔吸附树脂在GC-MS分析烟用加料香精挥发性组分的应用

<正>本实验以大孔吸附树脂为吸附剂,利用其对不同成分的选择性有吸附和筛选的作用,建立了通过大孔吸附树脂预处理烟用加料香精后GC-MS分析其中挥发性组分的方法。讨论了大孔吸附树脂与固相微萃取、直接进样等方法的优劣,确定了烟用香精样品经大孔吸附树脂处理后可以去除因溶剂峰过大而影响分析结果。     

超声波法提取青钱柳叶中的黄酮组分及其含量测定

本文以不同浓度的乙醇为溶剂,通过超声波法对青钱柳叶中的黄酮组分进行了提取,详细研究了乙醇浓度、体积、时间、温度对青钱柳叶中黄酮组分提取的影响;以芦丁为对照品,采用标准曲线法测定了青钱柳叶在不同提取条件下的黄酮含量。结果表明,使用超声波法提取青钱柳叶中黄酮组分的最佳条件为:乙醇浓度60%、超声时间35 min、超声温度35℃、固液比1:55。     

中药制药工业中大孔吸附树脂分离技术的应用研究

大孔吸附树脂是由吸附和筛选原理组合而成的分离材料。有机化合物所吸附力的不同和分子量的大小,在大孔吸附树脂上经过一定的溶剂洗脱而分开。这使得有机化合物尤其是水溶性化合物的提纯得以简化。但大孔吸附树脂分离效果受以下等众多因素制约。     

碱法提取仙人掌花黄酮抗肿瘤研究综述

经查阅国内外相关大量文献,仙人掌花中富含生物碱、多糖、黄酮等具有药疗价值的有机物,其中仙人掌花中黄酮类化合物在天然黄酮抗癌及临床等领域具有广泛应用。黄酮抗肿瘤作用机理主要是诱导细胞凋亡,抑制相关酶的活性等。仙人掌花中黄酮提取方法有有机溶剂提取、碱法、系统溶剂提取法等。实验拟用碱法提取仙人掌花黄酮,为后续开展仙人掌花黄酮研究打下基础。     

LSA20树脂吸附分离箭叶淫羊藿黄酮的研究

本文对LSA₂0树脂对箭叶淫羊藿黄酮的吸附分离性能进行研究。以黄酮为指标,采用分光光度法测定。浓缩液中总黄酮含量为65.21%。采用的测定方法简便、准确,可用于黄酮的含量测定。     

LSA_20树脂吸附分离箭叶淫羊藿黄酮的研究

本文采用LSA_20树脂吸附分离箭叶淫羊藿黄酮。以淫羊藿总黄酮和淫羊藿苷含量为指标研究淫羊藿的提取及LSA_20树脂分离工艺。以紫外分光光度法测定淫羊藿总黄酮的含量;以高效液相色谱法测定淫羊藿苷的含量;结果：淫羊藿总黄酮的最佳提取工艺为20,18倍量（V/W）,70%乙醇提取2次,提取时间为40,30分钟。上样浓度为6mg/mL,以流速3 BV/h上样,上样后静止一小时,用2 BV的去离子水洗去原液,然后用10倍树脂床体积的60%乙醇以3 BV/h流速洗脱,收集洗脱液。本实验方法成本低,适宜用于产业化生产。     

甲醇提取仙人掌中黄酮的正交优化工艺

<正>采用甲醇作溶剂提取仙人掌中的黄酮。用紫外-分光光度法测定提取的黄酮含量。正交试验考察了料液比(仙人掌干粉质量与提取剂体积之比,g·mL)、提取温度、提取时间和甲醇浓度对仙人掌中黄酮提取的影响。结果表明提取的最佳工艺条件为:料液比1:30(g/mL),提取温度60℃,提取时间3.5 h,甲醇浓度95%。上述条件下仙人掌中黄酮的提取率为0.741%。-1黄酮的功效是多方面的,近年来上市的保健产品中,很大一部分其中主要功效成分都属于黄酮类化合物,涉及功能食品的许多方面,其中以蜂胶、银杏、山楂、沙棘、荞麦、柑橘     

灵芝中总黄酮的含量测定

为了测定灵芝中总黄酮的含量,利用黄酮类化合物与金属离子形成的螯合物在510nm处有最大吸收度,用分光光度法进行测定,测得总黄酮含量为0.385%.效果满意,方法简便可靠.     

Response surface methodology based optimization for degradation of align in Laminaria japonica feedstuff via fermentation by Bacillus in Apostichopus japonicas farming

BackgroundThe alga Laminaria japonica is the most economically important brown seaweed cultured in China, which is used as food and aquatic animal feedstuff. However, the use of L. japonica as a feedstuff in Apostichopus japonicas farming is not ideal because A. japonicas does not produce enough enzyme activity for degrading the large amount of algin present in L. japonica. In this study, semi solid fermentation of the L. japonica feedstuff employing a Bacillus strain as the microbe was used to as a mean to degrade the algin content in L. japonica feedstuff.ResultsThe Bacillus strain, Bacillus amyloliquefaciens WB1, was isolated by virtue of its ability to utilize sodium alginate as the sole carbon source. Eight factors affecting growth and algin-degrading capacity of WB1 were investigated. The results of Plackett–Burman design indicated that fermentation time, beef extract, and solvent to solid ratio were the significant parameters. Furthermore, the mutual interaction between the solvent to solid ratio and beef extract concentration was more significant than the other pairs of parameters on algin degradation. Optimal values obtained from Central-Composite Design were 113.94 h for fermentation time, 0.3% (w/v) beef extract and 44.87 (v/w) ratio of solvent to feedstuff. Under optimal conditions, 56.88% of the algin was degraded when a 50-fold scale-up fermentation was carried out, using a 5-L fermenter.ConclusionsThis study provides an alternative and economical way to reduce the algin content in L. japonica through degradation by WB1, making it a promising potential source of feed for cultured L. japonica.     

部分冠折外伤性脱位牙的再植

为保存因外伤所致的伴有部分冠折的离体脱位牙,在对患儿体外根管充填治疗后,用螺纹钉加光因化复合树脂修复其缺损的牙冠,同时用ＰＨ为１．０的枸椽酸处理牙根表面,对１８例２１只患牙进行了即刻再植,结果表明,以往常被扫弃的伴有冠折的完全性脱位牙,在经过适当的治疗处理后可以再植成功。     

Photolysis of the d-glycosides: α benzylfructofuranoside, β benzylfructopyranoside,and α and β phenyl,benzyl and β phenylethyl glucosides; and the bearing of the data upon the transfer of energy within molecules

Direct absorption of light by glycosides dissolved in water has been discovered to produce changes in optical activities and formation of material which reduces the Shaffer, Hartmann, Somogyi reagent. Comparison of these measurements indicates in many cases the same products as are formed by hydrolysis in acid. The β glucosides and α fructoside, however, apparently give optically inactive photolytic products.Absorption spectra were obtained for α benzylfructofuranoside and β benzylfructopyranoside, and were found to be the same in the near ultraviolet as those previously obtained for benzyl glucosides and benzyl alcohol.The absorption spectrum for freshly purified fructose gives a deeper minimum about λ240 mμ than the previously reported values. The latter, however, were approached when the dry crystals had stood in air for several months although no change occurred in their sp.rot., m.p. or general apperance.All the glycosides studied were white crystalline compounds. Less than five per cent. of the ultraviolet light of λ254 mμ they absorb produces reaction; nevertheless, the apparatus and procedures available were adequate to study the reaction conveniently in monochromatic light of this wave-length.The absorption spectra, the products of photolysis, and the quantum yields, the latter based on the reducing material formed, lead to the hypothesis that the photochemical reaction is produced by an intramolecular transfer of absorbed energy from the aglycone to the hemiacetal oxygen bridge which is the reactive center. The efficiency of this transfer is, as expected, greater for benzyl than for phenylethyl glucosides but phenyl glucosides, contrariwise, are the least efficient.The structure and energy possessed by the glycoside outside the aglycone have no measurable effect on the efficiency of the photochemical reaction when followed by measuring the reducing material produced although they change up to one hundred thousand fold the rates of the thermal hydrolyses in acid.     

蔷薇科植物果核油性提取物体内外抗呼吸道病毒效果的观察

目的观察蔷薇科植物果核油性提取物体内外抗呼吸道病毒的效果。方法分别采用红细胞吸附抑制试验和细胞病变作用(CPE)检查,观察不同浓度油性提取物体外抗流感病毒A3株、冠状病毒OC43株和呼吸道合胞病毒E6株的作用。采用小鼠感染模型,观察不同浓度油性提取物滴鼻预防后,抵抗流感病毒A3株和冠状病毒OC43株致死性感染的效果。结果0.5%油性提取物作用1min、0.12%作用5min、0.06%作用10min均可完全抑制流感病毒A3株引起的鸡红细胞吸附现象;0.25%油性提取物作用1min、0.06%作用5min、0.03%作用10min均可完全抑制冠状病毒OC43株引起的小鼠红细胞吸附现象。1.0%油性提取物作用1min、0.25%作用5min、0.12%作用10min均可完全抑制呼吸道合胞病毒E6株引起的CPE。0.12%、0.25%和0.5%油性提取物滴鼻预防后,流感病毒A3株攻击小鼠的存活率分别为30%、90%和100%。0.06%、0.12%和0.25%油性提取物滴鼻预防后,冠状病毒OC43株攻击小鼠的存活率分别为30%、80%和100%。结论较低浓度的蔷薇科植物果核油性提取物即可显示出较强的体内外抗常见呼吸道病毒作用,但体内抗病毒作用有明显的有效剂量阈值。     

正交试验法确定黄芩的最佳水提工艺研究

确定黄芩的最佳水提工艺。方法:以出膏率、黄芩总黄酮和黄芩苷为指标,采用加水量、煎煮时间、煎煮次数和煎煮温度四因素、三水平L9(34)正交试验法,优选白芍的最佳水提工艺。结果:白芍的最佳水提工艺为:每次加10倍水,提取3次,每次提取2.0h。结论:确定的黄芩水提工艺使黄芩苷和黄芩苷的转移率分别达到117.1674mg/g和97.80%以上,说明确定的黄芩的水提工艺稳定可行。     

微波辅助提取虎杖中白藜芦醇的工艺研究

目的：优选微波辅助提取虎杖中白藜芦醇的最佳工艺。方法：用紫外分光光度法测定白藜芦醇的含量,并以白藜芦醇的提取率为参考指标,通过单因素实验及正交实验方法对微波辅助提取法（MAE）提取虎杖中白藜芦醇的最佳工艺进行研究。结果：经过正交实验得到从虎杖白藜芦醇的最佳工艺为：微波辅助作用时间15 min以70%的乙醇为提取剂,料液比1：30,微波辅助功率为500W。结论：该实验确定的最佳提取工艺简便、快速、准确,灵敏度高和重现性好等优点。     

基于显著区域选择和尺度空间主结构的视觉关注区域提取方法

基于显著区域选择和尺度空间主结构,提出了一种新颖的关注区域提取方法。模型中,关注区域提取方法分为三步：首先使用基于显著区域选择方法,利用对目标显著度贡献最大的特征估计图像中目标的大概位置;然后利用尺度空间主结构方法获得当前位置图像的重要结构区域范围以及合适的观测尺度;最后,将前两步中获得的区域范围合并起来作为最后的关注区域。实验结果和比较证明本文提出的模型能够获得较好的目标区域提取结果,更好地为识别模块服务。     

Antidyslipidemic Effect of Ocimum sanctum Leaf Extract in Streptozotocin Induced Diabetic Rats

The antidyslipidemic activity of Ocimum sanctum leaf extract was studied in streptozotocin induced diabetic rats. In this model, there was significant increase in plasma markers of diabetic-dyslipidemia following diminution of lipid metabolizing enzymes. Oral administration of leaf extract (500 mg/kg b.w.p.o.) for 15 days resulted in significant decrease in diabetogenic and dyslipidemia parameters; namely blood glucose, glycosylated hemoglobin, lipid peroxide, free fatty acids, small dense low density lipoprotein, lipid and protein components of plasma lipoproteins, adipose and liver. The regulation of lipids was accompanied by stimulation of postheparin lipolytic activity, reactivation of lecithin cholesterol acyl transferase and hepatic lipoprotein lipase enzymes. The results of the present study demonstrated antidyslipidemic and antioxidant activities in leaf extract of O. sanctum which could be used in prevention of diabetic-dyslipidemia and related complications.