Effects of vanadate on the activities of mice glucokinase and hexokinase

This study aimed at acquiring knowledge on the hypoglycemic mechanisms of sodium metavanadate (SMV) showed that the liver glucokinase and muscle hexokinase activities increased rapidly after oral SMV was given, and that the blood glucose level was correlated closely with the activities of the two enzymes but not with the insulin level; which indicated that SMV could improve the altered glucose phosphorylation in diabetic mice independently of stimulating insulin secretion. This was probably one of the mechanisms of hypoglycemic effects of SMV.     

Effects of breakfast with different calorigenic amounts on blood glucose, insulin and glucagon levels

This study was aimed to investigate the relationship between breakfast and serum glucose, insulin and glucagon concentrations in order to establish a model breakfast appropriate for Chinese. Twenty-four volunteers were randomly assigned to four study groups: high carbohydrate breakfast, high fat and protein breakfast, the typical breakfast and fasting. Each subject had serum and urine samples collected while fasting and at 1,2 and 3.5 hours following the meal. The concentration of serum glucose, insulin and glucagon was measured. The levels of serum glucose in group A,B and C differed significantly at 1 and 2 hour after meal compared to those at fasting (P<0.05). The serum glucose in group A increased insignificantly after meal. The serum insulin levels were in group A,B and C significant different compared with control group(P<0.05).Those peaked at 1 hour after meal ,with group C rising the furthest. Compared with the fasting group, the serum glucagons rose and maintained the increase after breakfast in group A,B and C (P<0.05).The data suggested that various diets with different calorigenic amounts increased hormone concentration to various extents. We found that a breakfast rich in carbohydrates could maintain proper blood glucose level.     

Effects of breakfast with different calorigenic amounts on blood glucose,insulin and glucagon levels

SUBJECTSANDMETHODSSubjectsTwenty four 2 1to 2 4yearsoldhealthymedi calstudentsenrolledinZhejiangUniversityCol legeofMedicinevolunteeredforthestudy .Theyconsistedof1 1malesand 1 3femalesweightsallwithinthenormalrangefortheirheightaccordingtoChinesenorms.Me…     

Effects of Hg and Cu on the activities of soil acid phosphatase

Comparative study on the activity and kinectic properties of acid phosphatase (ACPase) of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, Soil 1 was the most seriously contami- nated due to its lowest content of organic matter and the lowest pH among three samples, Soil 2 took the second place, and Soil 3 was the least contaminated. Except Soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may be a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.     

胰岛素休克小鼠血糖变化的研究

研究小鼠禁食不同时间及注射胰岛素导致小鼠休克时血糖的变化,并观察酸性生理盐水稀释胰岛素对低血糖休克出现时间及血糖值有无影响.结果表明,小鼠分别禁食12﹑18﹑22﹑24h时的血糖值之间无明显差异（P〉0.05）,在各组小鼠注射胰岛素后,血糖均明显下降（P〈0.05）,但各组下降后的血糖值之间无明显差异（P〉0.05）;不同剂量的胰岛素或是否用酸性生理盐水稀释胰岛素,注射后出现休克的时间及休克时各组小鼠血糖值之间无明显差异（P〉0.05）.     

溶剂胶束作用对葡萄糖氧化酶催化活性的影响

研究6种溶剂和表面活性剂对葡萄糖氧化酶催化活性的影响.研究表明,30%丙酮溶液对反应体系有激活作用,可使葡萄糖氧化酶测定葡萄糖的反应速率加快、灵敏度提高,线性范围达到0.00-10.00g/L,同时确定了最佳反应条件.研究结果对于优选新的激活剂具有一定的实际意义.     

蚁皇浆口服液抗衰老作用的实验研究

目的以衰老的自由基学说为依据,研究蚁皇浆口服液的抗衰老作用.方法52周龄小白鼠30只随机分为老龄实验组和老龄对照组,4周龄15只作为成年对照组,灌胃四周后分别取血测定SOD、CAT、Tch、TG和MDA等各项生化指标.结果老龄实验组SOD、CAT活性均明显升高(P＜0.01),MDA、Tch和TG含量明显降低(P＜0.01或P＜0.05).结论蚁皇浆口服液具有较强的抗氧化和抗衰老作用,是一种很有经济价值药食两用的保健品.     

知母多糖对家兔血糖的影响

[目的]〗研究知母多糖(Anemam,AM)对家兔血糖的影响.[方法]制备家兔高血糖模型,用不同剂量组的AM和D860后,测定对照组、AM组、D860组的血糖.[结果]AM1次灌胃2mg/kg,20mg/kg可显著降低四氧嘧啶引起的家兔血糖升高;AM1次灌胃20mg/kg对正常家兔血糖无明显影响,200mg/kg可降低正常家兔血糖.[结论]AM与D860同样具有实验性降低血糖作用.     

温度、氯化钠及乙醇对离子型表面活性剂临界胶束浓度的影响

表面活性剂的一个重要性质是其临界胶束浓度(Cfitical Mcelle Concentration，简称CMC)^[1]。本文利用电导率法分别对阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)和阴离子表面活性剂十二烷基硫酸钠(SDS)的CMC进行了研究，测试了这两种离子型表面活性剂在不同温度及添加不同量的无机盐NaCl和有机物乙醇时电导率变化，从而得到温度、NaCl、乙醇对CTAB、SDS的临界胶束浓度的影响规律，并对有关实验结果作了探讨。     

芸苔素内酯对平菇菌丝生长的影响

在PDA培养基和液体培养基中,加入适宜浓度0.06mg/L或0.06mg/L～0.10mg/L的芸苔素内酯,可促进平菇菌丝体的生长,延缓菌丝体衰老,延长菌种保藏时间.     

模拟高原环境对大鼠血清高密度脂蛋白胆固醇的影响

本实验在提高血清高密度脂蛋白胆固醇的方法方面作一初步探讨,观察了在平原环境有氧运动和模拟高原环境下大鼠血清高密度脂蛋白胆固醇水平的变化,主要结果如下:1、平原有氧运动能使血清高密度脂蛋白胆固醇显著升高,P<0.05;2、模拟高原环境下不训练能使血清高密度脂蛋白胆固醇升高非常显著P<0.01;3、模拟高原环境下进行有氧运动,血清高密度脂蛋白胆固醇升高极显著P<0.001,三组相比较提示:模拟高原训练在提高血清高密度脂蛋白胆固醇水平上更有意义,高原训练应用于冠心病病人的防治理论上是有意义的。     

Effects of atorvastatin on progression of diabetic nephropathy and local RAGE and soluble RAGE expressions in rats

Objective  

Advanced glycation end-products (AGEs) exert inflammatory and oxidative stress insults to produce diabetic nephropathy mainly through the receptor for AGEs (RAGE). This study aimed to assess the effect of atorvastatin on diabetic nephropathy via soluble RAGE (sRAGE) and RAGE expressions in the rat kidney.     

褐藻多糖硫酸酯影响血小板聚集及血栓形成

研究褐藻多糖硫酸酯对实验性出血、血栓形成及血小板聚集的影响 ;比较褐藻多糖硫酸酯给药后动物实验性出血时间及出血量的变化 ,比较褐藻多糖硫酸酯给药后动物实验性动、静脉血栓形成的变化 ,血小板聚集仪测定褐藻多糖硫酸酯对AA、ADP、CaCl2 、凝血酶诱导的血小板聚集的影响 ;褐藻多糖硫酸酯能够延长动物实验性出血时间并增加出血量 ,能够抑制动物实验性动、静脉血栓的形成 ,能够抑制体外诱导的血小板聚集 ;褐藻多糖硫酸酯对血栓形成具有抑制作用 ,该作用可能与抑制血小板聚集有关。     

Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro

Objective  

To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs).     

重金属镉对背角无齿蚌肝脏和肌肉过氧化氢酶活性的影响

研究了不同浓度的镉（Cd2＋）对背角无齿蚌（Anodonta woodiana woodiana）肝脏和肌肉过氧化氢酶（CAT）活性的影响.结果表明：在Cd2＋浓度低于0.7 mg·L-1时对肌肉CAT活性起诱导作用,在Cd2＋浓度低于0.5 mg·L-1时对肝脏CAT活性起诱导作用,最大值出现在0.2 mg·L-1附近;而高浓度Cd2＋（高于0.8 mg·L-1）对CAT起抑制作用.灵敏度结果显示,低浓度Cd2＋胁迫下（0.1 mg·L-1）,肝脏CAT的诱导倍数高于肌肉.背角无齿蚌肝脏CAT对水环境中的重金属反应敏感,对重金属的早期污染有指示作用.     

不同负荷运动大鼠补充胆红素后肝细胞凋亡趋势的研究

目的：观察补充胆红素对不同负荷运动造成的肝细胞凋亡的影响,为运动营养补剂的研发,减少运动性损伤提供一定的实验依据.方法：以53只健康雄性SD大鼠为研究对象,随机分成7个组,即安静对照组、中等负荷运动组、中等负荷运动＋胆红素组、大负荷运动组、大负荷运动＋胆红素组,力竭运动组、力竭运动＋胆红素组,通过实验检测肝细胞凋亡情况,测量肝组织SOD的活性和MDA含量.结果表明：力竭运动和大负荷运动能诱导大鼠肝细胞凋亡增加;补充胆红素使其肝细胞凋亡率降低,说明胆红素能提高机体抗氧化能力.     

非凝聚态粒子对超流临界速度实验值的影响

超流关键的物理量就是临界速度,MIT（麻省理工学院）实验组用激光来同扫描凝聚体的方法测出BEC超流体的临界速度,但是与理论值相比小得多．考虑到非凝聚态粒子的影响,发现MIT组测得的实验值并不是临界速度,而是玻色气体获得能量的最小速度,实际临界速度比测得的速度要大．通过修正玻色气体获得能量的速率公式从而得出临界速度与实验测量数据的关系,并发现修正后的临界速度的实验值符合理论值．     

汞污染对水稻幼苗生长及其过氧化物酶活性的影响

研究了不同浓度Hg~(2 )对水稻幼苗生长及其过氧化物酶活性的影响．结果表明,经Hg~(2 )胁迫的水稻幼苗,植株矮小,苗鲜重减少;低浓度Hg~(2 )使叶片叶绿素含量增加、POD活性升高．但随着Hg~(2 )浓度增大和处理天数增加,上述指标呈下降趋势,表明植物细胞遭受伤害．     

不同剂量美托洛尔对围术期心率和血压的作用

目的:探讨小剂量美托洛尔静脉注射时对围手术期室上性心动过速病人HR和BP的作用。方法:围手术期SBP≥110mmHg、HR≥120bpm为美托洛尔治疗指征。ASAⅠ～Ⅱ级病例90例分为组Ⅰ、组Ⅱ、组Ⅲ,分别静注美托洛尔1mg、2mg、3mg。记录每个病人用药前及用药后1、2、3、5、10min的HR、SBP、DBP、SPO_2。结果:组Ⅰ、Ⅱ、Ⅲ病人用药后1～5min HR减慢最为明显(P<0.01),平均下降的最低幅度分别达12.83％、20％和25.11％,10min后三组分别有80％、31％、18％病人心率增加至120bpm。SBP的变化基本与HR变化同步,1～3min下降最明显,5min后病人血压基奉平稳,下降的最大幅度平均为11.6％、15.74％、19.33％。各组DBP无明显变化。结论:静注美托洛尔1～3mg对围手术期心动过速有显著疗效。随着HR下降,SBP亦同步下降,而且与美托洛尔剂量成正比。对于围手术期间心动过速的病人,如果SBP≥130mmHg,建议使用3mg的剂量,用药后HR回升的现象较少;如果病人的SBP在120～130mmHg范围内,建议使用2mg;如果病人SBP在110～120mmHg,我们建议使用1mg;如果病人SBP<110mmHg,而HR>145次/min,可用0.5～1mg,并减慢注射速度,这样可避免美托洛尔所致的SBP下降过低。