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991.
古哈尔克孜·马合苏提 《内江科技》2011,32(3):99-99,104
本文主要阐述了盐渍土的定义及在盐渍土地区出现各种病害和对这些病害采用的处理措施等。 相似文献
992.
993.
中学数学教学的目的,归根结底在于培养学生的解题能力,提高数学解题能力是数学教学中一项十分重要的任务。提高学生解题能力始终贯穿于教学始终,我们必须把它放在十分重要的位置。教学关键是教会学生用所学的知识解决实际问题,即要提高学生的解题能力。文章从培养学生“数形”整合、“方程”思维、“对应”思维、“转化”能力、增强自信等五个方面谈如何培养学生的数学解题能力。那么,如何才能提高学生的解题能力,具体方法上讲主要可以从以下几方面入手: 相似文献
994.
针对如何培养学生的数学应用意识,提高学生应用数学知识解决问题的能力进行了分析。 相似文献
995.
在水处理中,水泵是极其重要的机器设备,它的正常和高效运行对整个水处理过程起着决定性的作用。但由于一般的污水处理中,水泵都处于高压高腐蚀性等环境,因此,它的安全使用技术是非常重要的。此外,水泵属于一种高能耗设备,在使用过程中的节能问题又是一个关键。本文就将重点探讨水泵在水处理中应该注意的节能性和安全性这两方面的技术问题。 相似文献
996.
工业生产或冷却工艺过程中产生的废热,一般要用冷却水来导走。冷却塔的作用是将挟带废热的冷却水在塔内与空气进行热交换,使废热传输给空气并散人大气。结合实际,重点介绍了新技术在冷却塔系统中的运用。 相似文献
997.
Edwards TL Harper JC Polsky R Lopez DM Wheeler DR Allen AC Brozik SM 《Biomicrofluidics》2011,5(4):44115-4411514
Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 °C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices. 相似文献
998.
Found in all eukaryotic cells, linker histones H1 are known to bind to and rearrange nucleosomal linker DNA. In vitro, the fundamental nature of H1∕DNA interactions has attracted wide interest among research communities-from biologists to physicists. Hence, H1∕DNA binding processes and structural and dynamical information about these self-assemblies are of broad importance. Targeting a quantitative understanding of H1 induced DNA compaction mechanisms, our strategy is based on using small-angle x-ray microdiffraction in combination with microfluidics. The usage of microfluidic hydrodynamic focusing devices facilitates a microscale control of these self-assembly processes, which cannot be achieved using conventional bulk setups. In addition, the method enables time-resolved access to structure formation in situ, in particular, to transient intermediate states. The observed time dependent structure evolution shows that the H1∕DNA interaction can be described as a two-step process: an initial unspecific binding of H1 to DNA is followed by a rearrangement of molecules within the formed assemblies. The second step is most likely induced by interactions between the DNA and the H1's charged side chains. This leads to an increase in lattice spacing within the DNA∕protein assembly and induces a decrease in the correlation length of the mesophases, probably due to a local bending of the DNA. 相似文献
999.
We present a microfluidic approach to characterizing temperature-dependent biomolecular interactions. Solvated L-arginine vasopressin (AVP) and its immobilized RNA aptamer (spiegelmer) were allowed to achieve equilibrium binding in a microchip at a series of selected temperatures. Unbound AVP were collected and analyzed with matrix-assisted laser desorption∕ionization mass spectrometry (MALDI-MS), yielding melting curves that reveal highly temperature-dependent zones in which affinity binding (36–45 °C) or dissociation (25–33 °C and 50–65 °C) occurs. Additionally, temperature-dependent binding isotherms were constructed; from these, thermodynamic quantities involved in binding were extracted. The results illustrated a strong change in heat capacity of interaction for this system, suggesting a considerable thermodynamic influence controlling vasopressin-spiegelmer interaction. 相似文献
1000.
In this paper, we present a movement-based assay to observe adaptability in Caenorhabditis elegans locomotion behavior. The assay comprises a series of sinusoidal microchannels with a fixed wavelength and modulating (increasing or decreasing) amplitude. The channel width is comparable to the body diameter of the organism. Worms are allowed to enter the channel from the input port and migrate toward the output port. Within channel sections that closely match the worm's natural undulations, the worm movement is relatively quick and steady. As the channel amplitude increases or decreases along the device, the worm faces difficulty in generating the propulsive thrust, begins to slow down and eventually fails to move forward. A set of locomotion parameters (i.e., average forward velocity, number and duration of stops, range of contact angle, and cut-off region) is defined for worm locomotion in modulated sinusoidal channels and extracted from the recorded videos. The device is tested on wild-type C. elegans (N2) and two mutants (lev-8 and unc-38). We anticipate this passive, movement-based assay can be used to screen nematodes showing difference in locomotion phenotype. 相似文献