阿伦·艾弗森十年

“一个人的青年永远属于他正年轻的年代,一个人的老去也永远属于他正在朽腐的年代。”十年生死两茫茫,不思量自难忘,十年,你不属于我我也不属于你,这世道谁和谁都不是永恒的朋友。一个朋友曾经跟我认真地说,所有的忠诚都期待回报。没错,从1996到2006,十年的光阴飞逝而过,阿伦·艾弗森坐在宾西法尼亚州费城某个酒吧的角落里,品味着那些曾经的岁月,曾经的故事,曾经的青春,曾经的篮球,曾经的这座城市以及曾经的76队,R＆B或者HIP-     

Effects of over-expressing resistin on glucose and lipid metabolism in mice

Resistin, a newly discovered peptide hormone mainly secreted by adipose tissues, is present at high levels in serum of obese mice and may be a potential link between obesity and insulin resistance in rodents. However, some studies of rat and mouse models have associated insulin resistance and obesity with decreased resistin expression. In humans, no relationship between resistin level and insulin resistance or adiposity was observed. This suggests that additional studies are necessary to determine the specific role of resistin in the regulation of energy metabolism and adipogenesis. In the present study, we investigated the effect of resistin in vivo on glucose and lipid metabolism by over-expressing resistin in mice by intramuscular injection of a recombinant eukaryotic expression vector pcDNA3.1-Retn encoding porcine resistin gene. After injection, serum resistin and serum glucose (GLU) levels were significantly increased in the pcDNA3.1-Retn-treated mice; there was an obvious difference in total cholesterol (TC) level between the experiment and the control groups on Day 30. In pcDNA3.1-Retn-treated mice, both free fatty acid (FFA) and high density lipoprotein (HDL) cholesterol levels were markedly lower than those of control, whereas HDL cholesterol and triglyceride (TG) levels did not differ between the two groups. Furthermore, lipase activity was expressly lower on Day 20. Our data suggest that resistin over-expressed in mice might be responsible for insulin resistance and parameters related to glucose and lipid metabolism were changed accordingly.     

The relations of parenting,effortful control,and ego control to children's emotional expressivity

The relations of observed parental warmth and positive expressivity and children's effortful control and ego control with children's high versus low emotional expressivity were examined in a 2-wave study of 180 children (M age = 112.8 months). There were quadratic relations between adults' reports of children's emotional expressivity and effortful control; moderate expressivity was associated with high effortful control. Structural equation models supported the hypothesis that children's ego overcontrol (versus undercontrol) mediated the relation between parental warmth or positive expressivity and children's emotional expressivity, although parenting at the follow-up did not uniquely predict in children's expressivity after controlling for the relations in these constructs over time. The alternative hypothesis that children's ego overcontrol elicited positive parenting and expressivity also was supported.     

王子游园记

从前,有一个自私的王子,他让人修了一座游乐场.却不让任何人进人 这里是我一个人的,不许其他人来玩!王子开心地在空荡荡的游乐场里玩耍。游乐场的大门紧紧地关着。天空听不到 笑声,花儿 看不到快乐游乐场是的人们,所聚集快乐以他们都不的地方,你开心了。却把大家都赶走了。于是,游乐场热闹起来。天空不再伤心,花儿也绽放出了笑脸。王子和朋友们开心地玩耍着。王子游园记@小Q     

寻回双腿的魅力

据说女人双腿最美的节季是 １８岁～２３岁，因为双腿在这时能呈现出最美的曲线。这一说法客观上有些道理，２０岁的女性已发育成熟，腿部显得丰满、颀长．浑圆、紧绷、可过几年结婚、怀孕，生了孩子后双腿顿显韶华尽褪，往日的风采荡然无存，让人一眼就看出生育过的印痕。在寒冷季节，这些你尚可借大衣长裤遮掩，在夏天就难免要原形毕露了。怎么办呢？     

Construction of recombinant industrial Saccharomyces cerevisiae strain with bglS gene insertion into PEP4 locus by homologous recombination

The bglS gene encoding endo-1,3-1,4-β-glucanase from Bacillus subtilis was cloned and sequenced in this study. The bglS expression cassette, including PGK1 promoter, bglS gene fused to the signal sequence of the yeast mating pheromone α-factor (MFals), and ADH1 terminator with G418-resistance as the selected marker, was constructed. Then one of the PEP4 allele of Saccharomyces cerevisiae WZ65 strain was replaced by bglS expression cassette using chromosomal integration of polymerase chain reaction (PCR)-mediated homologous recombination, and the bglS gene was expressed simultaneously. The recombinant strain S. cerevisiae (SC-βG) was preliminarily screened by the clearing hydrolysis zone formed after the barley β-glucan was hydrolyzed in the plate and no proteinase A (PrA) activity was measured in fermenting liquor. The results of PCR analysis ofgenome DNA showed that one of the PEP4 allele had been replaced and bglS gene had been inserted into the locus of PEP4 gene in recombinant strains. Different endo-1,3-1,4-β-glucanase assay methods showed that the recombinant strain SC-βG had high endo-1,3-1,4-β-glucanase expression level with the maximum of 69.3 U/(h-ml) after 60 h of incubation. Meanwhile, the Congo Red method was suitable for the determination of endo-1,3-1,4-β-glucanase activity during the actual brewing process. The current research implies that the constructed yeast strain could be utilized to improve the industrial brewing property of beer.     

产后体形恢复速成计划

女人谁不希望自己拥有一个健美窈窕的体形?所以怀孕和生产过程 令她们最担心的是怕产后会失去女性风姿,变得体态肥胖,臃肿难看。的确,绝大多数女性在未孕前身材苗条,乳房丰满,腰细如柳,十分富有曲线美,而产后很可能变得乳房松弛下坠,腹部隆起下垂,腰部粗圆,臀部宽大,一时难以恢复孕前状态。     

俏妈咪挑战有氧运动

经过了前几期的有氧练习,我们的产后美体塑身运动进入了最关键.也是最后的一步——体能训练。体能训练可以提升肌力以及肌持久力.紧缩生产时的肌肉松弛部分。恢复轻盈身材。     

Association of hypomethylation of LINE-1 repetitive element in blood leukocyte DNA with an increased risk of hepatocellular carcinoma

Global DNA hypomethylation has been associated with increased risk for cancers of the colorectum, bladder, breast, head and neck, and testicular germ cells. The aim of this study was to examine whether global hypomethylation in blood leukocyte DNA is associated with the risk of hepatocellular carcinoma (HCC). A total of 315 HCC cases and 356 age-, sex- and HBsAg status-matched controls were included. Global methylation in blood leukocyte DNA was estimated by analyzing long interspersed element-1 (LINE-1) repeats using bisulfite-polymerase chain reaction (PCR) and pyrosequencing. We observed that the median methylation level in HCC cases (percentage of 5-methylcytosine (5mC)=77.7%) was significantly lower than that in controls (79.5% 5mC) (P=0.004, Wilcoxon rank-sum test). The odds ratios (ORs) of HCC for individuals in the third, second, and first (lowest) quartiles of LINE-1 methylation were 1.1 (95% confidence interval (CI) 0.7–1.8), 1.4 (95% CI 0.8–2.2), and 2.6 (95% CI 1.7–4.1) (P for trend <0.001), respectively, compared to individuals in the fourth (highest) quartile. A 1.9-fold (95% CI 1.4–2.6) increased risk of HCC was observed among individuals with LINE-1 methylation below the median compared to individuals with higher (>median) LINE-1 methylation. Our results demonstrate for the first time that individuals with global hypomethylation measured in LINE-1 repeats in blood leukocyte DNA have an increased risk for HCC. Our data provide the evidence that global hypomethylation detected in the easily obtainable DNA source of blood leukocytes may help identify individuals at risk of HCC.