等你慢慢变老

有时候想，如果麦子不是像麦粒儿那么丁点儿，也许我会爱上他。我穿着平底鞋跟麦子站一块儿，他只高我指甲盖那么点儿。     

高中数学联赛(高考)中的复数变换

介绍复数解题的常用三种方法代数恒等化简,三角恒角变换,数形结合方法.     

So cold the witer is

<正>感恩节的时候给曲和发信息。我说感谢上帝给了我们这样的生活。曲曲很快回复我说,可是这样的生活什么时候才是尽头。我知道她一样坐在窗明几净的教室里,为了数学头疼不已。或许     

折叠月光

“现在想想真不该在这样的雪夜里投递包裹。”我嘀咕着,“都是因为客人承诺付出双倍价钱啊。”雪停了,夜晚的空气却更加寒冷。今天是月中,月亮又圆又亮,孤零零地挂在夜空,有不多的几颗小星远远地望着它。连星星也畏惧这寒冷偷偷躲起来了啊!     

探究式学习的七个认识误区

目前，我国新一轮义务教育课程改革强调在各科教学中开展探究式学习活动，这是一项重大的改革举措，也是本次课程教材改革一个夺目的“亮点”。为了实现新课程的培养目标，同时针对原有基础教育课程教材中存在的弊端，《基础教育课程改革纲要(试行)》提出     

男生女生缺点一箩筐

女生喜欢在背后说别人的坏话?女生动不动就哭鼻子?女生越来越野蛮了?这些都不再新鲜, 听听男生们是怎么评价女生的吧。     

当代高校公共艺术教育课程的反思与构建

公共艺术教育具有艺术教育、艺术的表演和校外艺术教育等公共性特征。调查表明,高校公共艺术教育课程没有硬性指标,课程内容存在层次差异与类型导向,课程设置表现为非均衡与自主性。后现代课程观背景下的高校公共艺术教育应构建合理的修学机制与课程模块;关注识读课程与探究性实践课程的双向开发;整合新教育理念与技术的课程;构建开放的课程平台与网络资源课程;创设后现代课程观的新型评价模式。     

韩国“伯乐”选“码”记

1994年3月25日,韩国总统金泳三来中国访问前,途经日本提出建议,中、日、韩三国有关单位应联合起来,共同开发和制造容易掌握的汉字文字处理计算机软件,使三国汉字意思转达和信息交换简单易行,以便有效地推进三国在经济、科学技术等领域的交流合作。随后,日本报纸报道,韩国学者发表文章说,韩国经济增长率低的原因在于“排斥汉字,采用朝鲜文字”。韩国总统和经济界也支持这种说法,并已着手准备恢复使用汉字。韩国三星集团在招收职工的考试中已经列入考试汉字的内容,并出版了连载汉字的报纸……在这些背景下,1994年11月中旬,中国中文信息学会负…     

Study on the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if h TRF1 mutation is one of the factors of the activation oftelomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing.Real-time RT-PCR was used to detect the expression ofhTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of h TRF1 in 10 cell line cells. Results: h TRF1 gene, mapped to 8q 13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108～0.0749) than in normal mononuclear cells (0.0493, 0.0369～0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1,2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.