Electromagnetic field exposure as a plausible approach to enhance the proliferation and differentiation of mesenchymal stem cells in clinically relevant scenarios

Mesenchymal stem/stromal cell(MSC)-based therapy has been regarded as one of the most revolutionary breakthroughs in the history of modern medicine owing to its myriad of immunoregulatory and regenerative properties.With the rapid progress in the fields of osteo-and musculoskeletal therapies,the demand for MSC-based treatment modalities is becoming increasingly prominent.In this endeavor,researchers around the world have devised new and innovative techniques to support the proliferation of MSCs while minimizing the loss of hallmark features of stem cells.One such example is electromagnetic field(EMF)exposure,which is an alternative approach with promising potential.In this review,we present a critical discourse on the efficiency,practicability,and limitations of some of the relevant methods,with insurmountable evidence backing the implementation of EMF as a feasible strategy for the clinically relevant expansion of MSCs.     

Finding the next ‘wave’ in internationalisation of higher education: focus on Malaysia

This article presents a case study of Malaysia’s inroad in internationalising its higher education system for the past three decades and proposes recommendations and the way forward in internationalisation. Internationalisation is one of the critical agenda in Malaysia’s higher education transformation with an end target of becoming an international hub of higher education excellence by 2020. The country is no stranger in internationalisation as efforts in student mobility, academic programmes and international collaboration have started since the 1980s and 1990s. As with other higher education systems globally, it was the private sector that initiated and sustained efforts in internationalisation of Malaysian higher education. With the growth in international student enrolment, the country has established itself as a student hub; however, greater focus and clarity in direction should be set forward in accelerating Malaysia’s progress in internationalisation, with research and development as a potential catalyst. The article also questions Malaysia’s current standing with regard to internationalisation and the need in facilitating higher education institutions to build their capacity in internationalisation, highlighting the important roles of individual institutions at both public and private sector that drive the country’s internationalisation agenda.     

Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Infectious bursal disease virus （IBDV） was inactivated by two different chemicals-formaldehyde and binary ethylenimine （BEI）. Formaldehyde was used at 0. 1% and 0.2%, while BEI was used at concentrations of 0.001 and 0.002 mol/L. These four vaccines were tested for their efficiency in generating humoral immune response in different groups of broiler chicks. Both BEI-inactivated vaccines gave relatively higher antibody titers and were almost twice as efficient as formaldehyde-inactivated ones.     

Characterization and Classification of Mesenchymal Stem Cells in Several Species Using Surface Markers for Cell Therapy Purposes

Mesenchymal stem cells are multipotent cells capable of replicating as undifferentiated cells, and have the potential of differentiating into mesenchymal tissue lineages such as osteocytes, adipocytes and chondrocytes. Such lineages can then be used in cell therapy. The aim of present study was to characterize bone marrow derived mesenchymal stem cells in four different species, including: sheep, goat, human and mouse. Human bone-marrow mesenchymal stem cells were purchased, those of sheep and goat were isolated from fetal bone marrow, and those of mouse were collected by washing bone cavity of femur and tibia with DMEM/F12. Using flow-cytometry, they were characterized by CD surface antigens. Furthermore, cells of third passage were examined for their osteogenic and adipogenic differentiation potential by oil red and alizarin red staining respectively. According to the results, CD markers studied in the four groups of mesenchymal stem cells showed a different expression. Goat and sheep expressed CD44 and CD166, and weakly expressed CD34, CD45, CD105 and CD90. Similarly, human and mouse mesenchymal cells expressed CD44, CD166, CD105 and CD90 whereas the expression of CD34 and CD45 was negative. In conclusion, although all mesenchymal stem cells display plastic adherence and tri-lineage differentiation, not all express the same panel of surface antigens described for human mesenchymal stem cells. Additional panel of CD markers are necessary to characterize regenerative potential and possible application of these stem cells in regenerative medicine and implantology.     

Using Active Learning Methods to Strategically Select Essays for Automated Scoring

Research on Automated Essay Scoring has become increasing important because it serves as a method for evaluating students’ written responses at scale. Scalable methods for scoring written responses are needed as students migrate to online learning environments resulting in the need to evaluate large numbers of written-response assessments. The purpose of this study is to describe and evaluate three active learning methods that can be used to minimize the number of essays that must be scored by human raters while still providing the data needed to train a modern Automated Essay Scoring system. The three active learning methods are the uncertainty-based, the topological-based, and the hybrid method. These three methods were used to select essays included in the Automated Student Assessment Prize competition that were then classified using a scoring model that was trained with the bidirectional encoder representations from a transformer language model. All three active learning methods produced strong results, with the topological-based method producing the most efficient classification. Growth rate accuracy was also evaluated. The active learning methods produced different levels of efficiency under different sample size allocations but, overall, all three methods were highly efficient and produced classifications that were similar to one another.     

‘Cracks’ in the scholarly communications system: Insights from a longitudinal international study of early career researchers

• 170 early career researchers interviewed three times over 2 years, have uniquely contributed towards a stress test of scholarly communications and cracks have been identified.
• The perfect storm created by the convergence of millennial values and the pandemic appears to have fast-forwarded the cracking process, perhaps, for the good.
• The cracks in question are: (1) peer review; (2) reputational assessment; (3) unethical/questionable practices; (4) collaboration; (5) networking.