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Melinda G. Simon Ying Li Janahan Arulmoli Lisa P. McDonnell Adnan Akil Jamison L. Nourse Abraham P. Lee Lisa A. Flanagan 《Biomicrofluidics》2014,8(6)
Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem and progenitor cells owing to its ability to sort cells in a label-free manner and its biological safety. However, DEP separation devices have suffered from a low throughput preventing researchers from undertaking studies requiring large numbers of cells, such as needed for cell transplantation. We developed a microfluidic device designed for the enrichment of stem and progenitor cell populations that sorts cells at a rate of 150,000 cells/h, corresponding to an improvement in the throughput achieved with our previous device designs by over an order of magnitude. This advancement, coupled with data showing the DEP-sorted cells retain their enrichment and differentiation capacity when expanded in culture for periods of up to 2 weeks, provides sufficient throughput and cell numbers to enable a wider variety of experiments with enriched stem and progenitor cell populations. Furthermore, the sorting devices presented here provide ease of setup and operation, a simple fabrication process, and a low associated cost to use that makes them more amenable for use in common biological research laboratories. To our knowledge, this work represents the first to enrich stem cells and expand them in culture to generate transplantation-scale numbers of differentiation-competent cells using DEP. 相似文献
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Comparative international tests of students' achievements have become increasingly popular over the past decade. An outcome of this widespread practice is the tendency of various countries to evaluate their national status according to their pupils' international ranking in such achievement tests, partly due to the common belief that high ranking is an indication of the nation's future ability to compete in the global market. Using PISA 2000 and PISA 2006 data, the present study aims to show that the low achievements of Israeli pupils were to be expected, and that their average scores are a reflection of the economic and demographic characteristics of their country. Our findings suggest that the public debate in Israel on school achievements has been magnified out of all proportion. We also discuss the educational policy which derives proposals for educational reform from a conception of globalisation of education. This policy focuses on the setting of standards for educational achievements aimed at improving pupils' place on the international scale. Finally, we claim that the focus on the international grading of educational achievements deflects public attention from an understanding of the significance of inequalities within the country. 相似文献
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Geraldine Mooney Simmie Joanne Moles 《Mentoring & Tutoring: Partnership in Learning》2013,21(4):465-482
In this paper, we report on a mentoring programme which provides an accreditation pathway to a master’s level qualification. The paper served three purposes. First, informed by selected literature on education we caution against expedient reductionist models that are based solely on novice–expert mentoring relationships with limited facility for critical inquiry. Second, we present an evolving theoretical framework for productive mentoring based on our critique of a preferred academic literature and interactions with mentor teachers, school principals and teacher educators. We proactively encouraged an awareness of societal norms and traditions that can appear as counterculture to critical thinking. Lastly, we consider some implications for productive mentoring as an academic, caring and professional practice within a continuum of teacher education. 相似文献
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Stereotypes about aging and the old, both negative and positive, have significant influence upon older people themselves. Often unknowingly, older people incorporate these stereotypes into their own thinking and self-perceptions. A literature search revealed four primary hypotheses about the manner in which these self-stereotypes impact older people: stereotype threat, comparison, externalization, and internalization. Anecdotal examples of the four hypotheses are drawn from an unrelated research study that the authors are currently undertaking. Among these hypotheses, four basic characteristics of self-stereotypes are identified: (a) stereotypes can be negative or positive; (b) stereotypes have significant power, particularly if they are self-relevant; (c) stereotypes can be operative without awareness and influence physical and cognitive outcomes; and (d) negative stereotypes can be countered by positive priming. Due to their ability to significantly influence the cognitive and functional well-being of older people, further research is essential to better understand how these self-stereotypes function. 相似文献
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This study identifies qualities associated with the successful adaptation of families with a husband diagnosed with prostate cancer. Both qualitative and quantitative measures were used in this cross-sectional survey research design. Twenty-one husbands and their spouses independently completed six questionnaires and a biographical questionnaire, and they answered an open-ended question. The qualitative findings revealed the importance of intrafamilial support, spiritual/religious beliefs, and professional support and knowledge, while the quantitative results indicated that family adaptation was fostered by the family's internal strengths and durability, affirmative communication, and the experience of social support. The findings could be used to develop interventions that promote family resilience and affirm the reparative potential of families. 相似文献
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