Association of Elevated Serum Lipoprotein(a), Inflammation,Oxidative Stress and Chronic Kidney Disease with Hypertension in Non-diabetes Hypertensive Patients

Hypertension is the most common cardiovascular risk factor. Lipoprotein(a) [Lp(a)], inflammation, oxidative stress and chronic kidney disease (CKD) exacerbate the response to tissue injury and acts as markers of the vascular disease, especially in glomerulosclerosis. We compared the clinical characteristics of 138 non-diabetes hypertensive women (ndHT) patients with 417 non-diabetes normotensive subjects and tested the association of hypertension with Lp(a), inflammation, CKD and oxidative stress by using multiple logistic regression. BP, BMI, waist circumference, creatinine, Lp(a), inflammation and malondialdehyde levels were significantly higher and CKD state in the ndHT patients (p < 0.05). Multiple logistic regression showed hypertension associated with increased Lp(a), inflammation, ORs and 95 % CIs were 2.52 (1.33, 4.80), 2.75 (1.44, 5.27) after adjusting for their covariates. Elevated serum Lp(a) and inflammation levels concomitants with increased oxidative stress and CKD were the major risk factors associated with hypertension and implications for the increased risk of HT and vascular disease.     

The effect of extremely high glucose concentrations on 21 routine chemistry and thyroid Abbott assays: interference study

Introduction

Extremely high glucose concentrations have been shown to interfere with creatinine assays especially with Jaffe method in peritoneal dialysate. Because diabetes is the fastest growing chronic disease in the world, laboratories study with varying glucose concentrations. We investigated whether different levels of glucose spiked in serum interfere with 21 routine chemistry and thyroid assays at glucose concentrations between 17-51 mmol/L.

Materials and methods

Baseline (group I) serum pool with glucose concentration of 5.55 (5.44-5.61) mmol/L was prepared from patient sera. Spiking with 20% dextrose solution, sample groups were obtained with glucose concentrations: 17.09, 34.52, and 50.95 mmol/L (group II, III, IV, respectively). Total of 21 biochemistry analytes and thyroid tests were studied on Abbott c8000 and i2000sr with commercial reagents. Bias from baseline value was checked statistically and clinically.

Results

Creatinine increased significantly by 8.74%, 31.66%, 55.31% at groups II, III, IV, respectively with P values of < 0.001. At the median glucose concentration of 50.95 mmol/L, calcium, albumin, chloride and FT4 biased significantly clinically (-0.85%, 1.63%, 0.65%, 7.4% with P values 0.138, 0.214, 0.004, < 0.001, respectively). Remaining assays were free of interference.

Conclusion

Among the numerous biochemical parameters studied, only a few parameters are affected by dramatically increased glucose concentration. The creatinine measurements obtained in human sera with the Jaffe alkaline method at high glucose concentrations should be interpreted with caution. Other tests that were affected with extremely high glucose concentrations were calcium, albumin, chloride and FT4, hence results should be taken into consideration in patients with poor diabetic control.Key words: assay interference, glucose interference, preanalytical phase, creatinine, Jaffe kinetic assay, thyroid function tests     

Understanding researchers: findings from a focus group

In November 2006, a focus group of researchers was assembled at a conference hosted by the Society of Scholarly Publishers in Washington, DC. The panel addressed a series of prepared questions regarding current research practices, perceptions, and preferences, particularly as they applied to scientific and medical publications, in corporate, academic, and medical environments. This article highlights issues and views the panel discussed, with the aim of narrowing the gap of understanding between researchers and publishers. This information will help publishers to develop content and delivery methods that are closely aligned with the needs of researchers.     

Influence of upright versus time trial cycling position on determination of critical power and W′ in trained cyclists

Body position is known to alter power production and affect cycling performance. The aim of this study was to compare mechanical power output in two riding positions, and to calculate the effects on critical power (CP) and W′ estimates. Seven trained cyclists completed three peak power output efforts and three fixed-duration trial (3-, 5- and 12-min) riding with their hands on the brake lever hoods (BLH), or in a time trial position (TTP). A repeated-measures analysis of variance showed that mean power output during the 5-min trial was significantly different between BLH and TTP positions, resulting in a significantly lower estimate of CP, but not W′, for the TTP trial. In addition, TTP decreased the performance during each trial and increased the percentage difference between BLH and TTP with greater trial duration. There were no differences in pedal cadence or heart rate during the 3-min trial; however, TTP results for the 12-min trial showed a significant fall in pedal cadence and a significant rise in heart rate. The findings suggest that cycling position affects power output and influences consequent CP values. Therefore, cyclists and coaches should consider the cycling position used when calculating CP.     

IPv6: The next generation Internet Protocol

IPv4, the workhorse protocol of the currently popular TCP/ IP protocol suite, is fast becoming obsolete. The exponential growth of the Internet is the main reason that has required the creation of the next generation of Internet Protocol-IPv6. IPv6 is much more flexible and promises to take care of the address space and security issues in the foreseeable future. Part 1 of the paper lays the foundation of the various issues involved in designing the Internet Protocol and motivates the need for an upgraded version of this protocol.     

Coordination variability and skill development in expert triple jumpers

The aim of this study was to examine the influence of skill of expert triple jumpers on the coordination variability of lower extremity intra-limb couplings. In contrast to the traditional motor learning perspective, we hypothesized that as skill and thus performance increases, movement coordination variability will also increase. Three-dimensional kinematic and ground reaction force data were collected during the hop-step transition phase of the triple jump. Relative motion plots and a modified vector coding technique were used to quantify the coordination variability across the trials. The results were consistent with a U-shaped curve, representing coordination variability, as skill increases. The high coordination variability in less skilled athletes is present while the appropriate characteristics defining the movement coordination patterns are acquired. This coordination variability may not be beneficial to performance. As the refinement of these characteristics is achieved, coordination variability decreases, resulting in a more consistent or regulated performance. In the final stages of developing a skilled performance, a functional variability is accessed that brings flexibility to the system allowing it to cope with perturbations. This study highlights the need to address the learning effect when analysing coordination variability from a dynamical systems perspective.     

The influence of lower limb amputation level on the approach in the amputee long jump

In this study, we investigated the adjustments to posture, kinematic and temporal characteristics of performance made by lower limb amputees during the last few strides in preparation for long jump take-off. Six male unilateral trans-femoral and seven male unilateral trans-tibial amputees competing in a World Championships final were filmed in the sagittal plane using a 100-Hz digital video camera positioned so that the last three strides to take-off were visible. After digitizing using a nine-segment model, a range of kinematic variables were computed to define technique characteristics. Both the trans-femoral and trans-tibial athletes appeared to achieve their reduction in centre of mass during the flight phase between strides, and did so mainly by extending the flight time by increasing stride length, achieved by a greater flexion of the hip joint of the touch-down leg. The trans-tibial athletes appeared to adopt a technique similar to that previously reported for able-bodied athletes. They lowered their centre of mass most on their second last stride (-1.6% of body height compared with -1.4% on the last stride) and used a flexed knee at take-off on the last stride, but they were less able to control their downward velocity at touch-down (-0.4 m x s(-1)). Both this and their restricted approach speed (8.9 m x s(-1) at touch-down), rather than technique limitations, influenced their jump performance. The trans-femoral athletes lowered their centre of mass most on the last stride (-2.3% of body height compared with -1.6% on the second last stride) and, as they were unable to flex their prosthetic knee sufficiently, achieved this by abducting their prosthetic leg during the support phase, which led to a large downward velocity at touch-down (-0.6 m x s(-1)). This, combined with their slower approach velocity (7.1 m x s(-1) at touch-down), restricted their performance.